TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440806.2.1
(1136 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BU995294.1|BU995294 HM09M17r HM Hordeum vulgare subsp. v... 201 6e-080
gb|BI960587.1|BI960587 HVSMEn0025E05f Hordeum vulgare rachi... 271 5e-072
gb|BU981349.1|BU981349 HA23F03r HA Hordeum vulgare subsp. v... 268 4e-071
gb|BM442096.2|BM442096 EBan01_SQ002_C23_R anther, yellow st... 207 8e-053
gb|CB881433.1|CB881433 HM09M17w HM Hordeum vulgare subsp. v... 173 2e-042
gb|AV915392.1|AV915392 AV915392 K. Sato unpublished cDNA li... 163 1e-039
gb|CA001634.1|CA001634 HS05C08r HS Hordeum vulgare subsp. v... 158 6e-038
gb|BI959076.1|BI959076 HVSMEn0018A17f Hordeum vulgare rachi... 101 9e-028
>gb|BU995294.1|BU995294 HM09M17r HM Hordeum vulgare subsp. vulgare cDNA clone HM09M17
5-PRIME, mRNA sequence
Length = 585
Score = 201 bits (433), Expect(2) = 6e-080
Identities = 78/96 (81%), Positives = 85/96 (88%)
Frame = -3 / +1
Query: 744 KDREMQVTVAFNHFGKGLVQRMPRCRHGFFHVVNNDYTHWLMYAIGGSRNPTIISQGNRF 565
+DR MQVTVAFNHFGKGLVQRMPRCR GFFHVVNNDYTHW MYAIGG+R+PTIISQGNRF
Sbjct: 298 QDRMMQVTVAFNHFGKGLVQRMPRCRFGFFHVVNNDYTHWKMYAIGGNRDPTIISQGNRF 477
Query: 564 RAVDDSRFKEVTKREYTQYSEYKNWVWKSQDDLFLN 457
A DD KEVTKREYT Y+EYK+WVWKSQ D+ +N
Sbjct: 478 IAPDDPNAKEVTKREYTPYNEYKDWVWKSQGDVMMN 585
Score = 172 bits (370), Expect(2) = 3e-075
Identities = 74/91 (81%), Positives = 76/91 (83%)
Frame = +2 / -2
Query: 470 SSCDFHTQFLYSLYCVYSRLVTSLNLLSSTARKRLPWLMMVGFRLPPMAYMSQCV*SLFT 649
S CDFHTQ LYSL VYSRLVTSL L SS A R PWLMMVG RLPPMAY+ QCV*SLFT
Sbjct: 572 SPCDFHTQSLYSL*GVYSRLVTSLALGSSGAMNRFPWLMMVGSRLPPMAYIFQCV*SLFT 393
Query: 650 TWKKP*RQRGIRCTSPLPKWLKATVTCISLS 742
TWKKP RQRG+RCT PLPKWLKATVTCI LS
Sbjct: 392 TWKKPKRQRGMRCTRPLPKWLKATVTCIILS 300
Score = 130 bits (279), Expect(2) = 3e-075
Identities = 56/86 (65%), Positives = 59/86 (68%)
Frame = +2 / -2
Query: 752 ASLAPNSITWSCLVKWPLETVMAVEPSTASTSPSAQLLMDRWSIQISLDPITETPSPSLS 931
A LAP S+TWS LVKW LETVMAVEP T S PSAQ+ MD WSI L+ ETPSPSL
Sbjct: 287 ALLAPKSMTWSWLVKWLLETVMAVEPLTTSMRPSAQVDMDTWSIHTLLEERMETPSPSLR 108
Query: 932 PRTPQWCCESRIAPPPCAAASWTCRL 1009
R P WC ESRI PP C A WTC L
Sbjct: 107 VRRP*WCLESRIMPPECGAEWWTCTL 30
Score = 117 bits (250), Expect(2) = 6e-080
Identities = 46/79 (58%), Positives = 55/79 (69%)
Frame = -3 / +1
Query: 966 IRDSQHHWGVRXXXXXXXXXXXXXXDIWIDHLSMSSCADGLVDAVDGSTAITVSNGHFTR 787
IRDS+HH+G R ++WIDH+SMS+CADGL+D V GSTAITVSN HFT
Sbjct: 73 IRDSKHHYGRRTRSDGDGVSILSSSNVWIDHVSMSTCADGLIDVVSGSTAITVSNSHFTN 252
Query: 786 HDHVMLFGASDAASKDREM 730
HDHVMLFGAS+A + M
Sbjct: 253 HDHVMLFGASNAQEQQDRM 309
>gb|BI960587.1|BI960587 HVSMEn0025E05f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0025E05f, mRNA sequence
Length = 663
Score = 271 bits (586), Expect = 5e-072
Identities = 108/185 (58%), Positives = 133/185 (71%)
Frame = -3 / +1
Query: 888 IWIDHLSMSSCADGLVDAVDGSTAITVSNGHFTRHDHVMLFGASDAASKDREMQVTVAFN 709
+W+DH S+S+CADGL+DA+ GSTAITVSN +FT H+ VML G SD+ KD+ MQVT+AFN
Sbjct: 43 VWVDHCSLSNCADGLIDAIMGSTAITVSNNYFTHHNEVMLLGHSDSYLKDKAMQVTIAFN 222
Query: 708 HFGKGLVQRMPRCRHGFFHVVNNDYTHWLMYAIGGSRNPTIISQGNRFRAVDDSRFKEVT 529
HFG+GL+QRMPRCRHG+FHVVNNDYTHW MYAIGGS PTI SQGNR+ A + KEVT
Sbjct: 223 HFGEGLIQRMPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYLAPTNPFAKEVT 402
Query: 528 KREYTQYSEYKNWVWKSQDDLFLNGAFFNQSGGQNERKYDRLDLIQAKGGQYAESLTRYA 349
KR T + +K W W+S+ D+ LNGAFF S Y R + K ++T A
Sbjct: 403 KRVETAQTTWKAWNWRSEGDMLLNGAFFTPSRAXASAIYSRAXSLGGKSPSMVATITSGA 582
Query: 348 GALNC 334
GAL+C
Sbjct: 583 GALSC 597
Score = 164 bits (352), Expect = 1e-039
Identities = 85/155 (54%), Positives = 97/155 (62%)
Frame = +2 / -2
Query: 437 DWLKKAPLRNRSSCDFHTQFLYSLYCVYSRLVTSLNLLSSTARKRLPWLMMVGFRLPPMA 616
D +KKAP + S D Q + ++ V + VTSL AR R PWL+MVG LPPMA
Sbjct: 494 DGVKKAPFSSMSPSDLQFQAFHVVWAVSTLFVTSLAKGLVGAR*RFPWLLMVGSALPPMA 315
Query: 617 YMSQCV*SLFTTWKKP*RQRGIRCTSPLPKWLKATVTCISLSLDAASLAPNSITWSCLVK 796
Y+SQ V*SLFTTWK P GI SP PKWLKA VTCI+LSL SL P SIT VK
Sbjct: 314 YISQ*V*SLFTTWK*PCLHLGILWMSPSPKWLKAMVTCIALSLR*ESL*PRSITSLWWVK 135
Query: 797 WPLETVMAVEPSTASTSPSAQLLMDRWSIQISLDP 901
L+TVMAVEP AS PSAQL ++WS Q L P
Sbjct: 134 *LLDTVMAVEPMMASMRPSAQLEREQWSTQTWLAP 30
>gb|BU981349.1|BU981349 HA23F03r HA Hordeum vulgare subsp. vulgare cDNA clone HA23F03
5-PRIME, mRNA sequence
Length = 509
Score = 268 bits (580), Expect = 4e-071
Identities = 105/138 (76%), Positives = 116/138 (84%)
Frame = -3 / +3
Query: 726 VTVAFNHFGKGLVQRMPRCRHGFFHVVNNDYTHWLMYAIGGSRNPTIISQGNRFRAVDDS 547
VTVAFNHFGKGLVQRMPRCR GFFHVVNNDYTHW MYAIGG+R+PTIISQGNRF A DD
Sbjct: 12 VTVAFNHFGKGLVQRMPRCRFGFFHVVNNDYTHWKMYAIGGNRDPTIISQGNRFIAPDDP 191
Query: 546 RFKEVTKREYTQYSEYKNWVWKSQDDLFLNGAFFNQSGGQNERKYDRLDLIQAKGGQYAE 367
KEVTKREYT Y+EYK+WVWKSQ D+ +NGAFFNQSGGQNER Y +D I AK G+Y
Sbjct: 192 NAKEVTKREYTPYNEYKDWVWKSQGDVMMNGAFFNQSGGQNERTYGNMDFIPAKHGKYVG 371
Query: 366 SLTRYAGALNCRVGRKC* 313
LT++AG L CRVG+ C*
Sbjct: 372 QLTQFAGTLECRVGKPC* 425
Score = 238 bits (514), Expect = 5e-062
Identities = 105/154 (68%), Positives = 113/154 (73%)
Frame = +2 / -2
Query: 266 RSIANDESCSLELHTH*HFLPTRQLSAPAYLVSDSAYWPPLAWMRSSLSYLRSFWPPDWL 445
R + N ++ + H*H LPTR S PA VS Y P LA M+S L Y+RSFWPPDWL
Sbjct: 472 RQLINWQARRDAIEFH*HGLPTRHSSVPANCVSCPTYLPCLAGMKSMLPYVRSFWPPDWL 293
Query: 446 KKAPLRNRSSCDFHTQFLYSLYCVYSRLVTSLNLLSSTARKRLPWLMMVGFRLPPMAYMS 625
KKAP S CDFHTQ LYSL VYSRLVTSL L SS A R PWLMMVG RLPPMAY+
Sbjct: 292 KKAPFIMTSPCDFHTQSLYSL*GVYSRLVTSLALGSSGAMNRFPWLMMVGSRLPPMAYIF 113
Query: 626 QCV*SLFTTWKKP*RQRGIRCTSPLPKWLKATVT 727
QCV*SLFTTWKKP RQRG+RCT PLPKWLKATVT
Sbjct: 112 QCV*SLFTTWKKPKRQRGMRCTRPLPKWLKATVT 11
>gb|BM442096.2|BM442096 EBan01_SQ002_C23_R anther, yellow stage, no treatment, cv Optic,
EBan01 Hordeum vulgare subsp. vulgare cDNA clone
EBan01_SQ002_C23 5', mRNA sequence
Length = 356
Score = 207 bits (447), Expect = 8e-053
Identities = 84/116 (72%), Positives = 94/116 (81%)
Frame = -3 / +2
Query: 744 KDREMQVTVAFNHFGKGLVQRMPRCRHGFFHVVNNDYTHWLMYAIGGSRNPTIISQGNRF 565
KD+ MQVTVAFNHFG+GLVQRMPRCR GFFHVVNNDYTHWLMYAIGGS+NPTIISQGNR+
Sbjct: 8 KDQIMQVTVAFNHFGRGLVQRMPRCRWGFFHVVNNDYTHWLMYAIGGSKNPTIISQGNRY 187
Query: 564 RAVDDSRFKEVTKREYTQYSEYKNWVWKSQDDLFLNGAFFNQSGGQNERKYDRLDL 397
A + K +TKR +E+KNWVW SQDDL LN A F SGG NERK+D+ DL
Sbjct: 188 IAPPNLAAKLITKRLGASEAEWKNWVWHSQDDLLLNDAVFADSGGANERKFDKDDL 355
Score = 153 bits (328), Expect = 2e-036
Identities = 72/116 (62%), Positives = 76/116 (65%)
Frame = +2 / -3
Query: 398 RSSLSYLRSFWPPDWLKKAPLRNRSSCDFHTQFLYSLYCVYSRLVTSLNLLSSTARKRLP 577
RSSLS S PP K A +RSSC+ HTQF +S SRLV S A R P
Sbjct: 354 RSSLSNFLSLAPPLSAKTASFSSRSSCECHTQFFHSASDAPSRLVISFAARFGGAM*RFP 175
Query: 578 WLMMVGFRLPPMAYMSQCV*SLFTTWKKP*RQRGIRCTSPLPKWLKATVTCISLSL 745
WLMMVGF LPPMAYMSQ V*SLFTTWK P R GI CT PLPKWLKATVTC+ SL
Sbjct: 174 WLMMVGFLLPPMAYMSQWV*SLFTTWKNPHRHLGILCTRPLPKWLKATVTCMIWSL 7
>gb|CB881433.1|CB881433 HM09M17w HM Hordeum vulgare subsp. vulgare cDNA clone HM09M17
3-PRIME, mRNA sequence
Length = 490
Score = 173 bits (372), Expect = 2e-042
Identities = 69/101 (68%), Positives = 80/101 (79%)
Frame = -3 / -1
Query: 615 AIGGSRNPTIISQGNRFRAVDDSRFKEVTKREYTQYSEYKNWVWKSQDDLFLNGAFFNQS 436
AIGG+ +PTIISQGNRF A DD KEVTKREYT Y+EYK+WVWKSQ D+ +NGAFFNQS
Sbjct: 490 AIGGNPDPTIISQGNRFIAPDDPNAKEVTKREYTPYNEYKDWVWKSQGDVMMNGAFFNQS 311
Query: 435 GGQNERKYDRLDLIQAKGGQYAESLTRYAGALNCRVGRKC* 313
GGQNER Y +D I AK G+Y LT++AG L CRVG+ C*
Sbjct: 310 GGQNERTYGNMDFIPAKHGKYVGQLTQFAGTLECRVGKPC* 188
Score = 153 bits (329), Expect = 2e-036
Identities = 71/116 (61%), Positives = 77/116 (66%)
Frame = +2 / +3
Query: 266 RSIANDESCSLELHTH*HFLPTRQLSAPAYLVSDSAYWPPLAWMRSSLSYLRSFWPPDWL 445
R + N ++ + H*H LPTR S PA VS Y P LA M+S L Y+RSFWPPDWL
Sbjct: 141 RQLINWQARRDAIEFH*HGLPTRHSSVPANCVSCPTYLPCLAGMKSMLPYVRSFWPPDWL 320
Query: 446 KKAPLRNRSSCDFHTQFLYSLYCVYSRLVTSLNLLSSTARKRLPWLMMVGFRLPPM 613
KKAP S CDFHTQ LYSL VYSRLVTSL L SS A R PWLMMVG LPPM
Sbjct: 321 KKAPFIMTSPCDFHTQSLYSL*GVYSRLVTSLALGSSGAMNRFPWLMMVGSGLPPM 488
>gb|AV915392.1|AV915392 AV915392 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags14d11 5', mRNA sequence
Length = 554
Score = 163 bits (351), Expect = 1e-039
Identities = 67/123 (54%), Positives = 79/123 (64%)
Frame = -3 / +3
Query: 681 MPRCRHGFFHVVNNDYTHWLMYAIGGSRNPTIISQGNRFRAVDDSRFKEVTKREYTQYSE 502
MPRCRHG+FHVVNNDYTHW MYAIGGS PTI SQGNR+ A + KEVTKR T +
Sbjct: 21 MPRCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYLAPTNPFAKEVTKRVETAQTT 200
Query: 501 YKNWVWKSQDDLFLNGAFFNQSGGQNERKYDRLDLIQAKGGQYAESLTRYAGALNCRVGR 322
+K W W+S+ D+ LNGAFF SG Y R + AK ++T AGAL+C G
Sbjct: 201 WKAWNWRSEGDMLLNGAFFTPSGAGASASYSRASSLGAKSSSMVATITSGAGALSCHKGS 380
Query: 321 KC* 313
C*
Sbjct: 381 SC* 389
>gb|CA001634.1|CA001634 HS05C08r HS Hordeum vulgare subsp. vulgare cDNA clone HS05C08
5-PRIME, mRNA sequence
Length = 521
Score = 158 bits (339), Expect = 6e-038
Identities = 65/121 (53%), Positives = 77/121 (63%)
Frame = -3 / +1
Query: 675 RCRHGFFHVVNNDYTHWLMYAIGGSRNPTIISQGNRFRAVDDSRFKEVTKREYTQYSEYK 496
RCRHG+FHVVNNDYTHW MYAIGGS PTI SQGNR+ A + KEVTKR T + +K
Sbjct: 13 RCRHGYFHVVNNDYTHWEMYAIGGSAEPTINSQGNRYLAPTNPFAKEVTKRVETAQTTWK 192
Query: 495 NWVWKSQDDLFLNGAFFNQSGGQNERKYDRLDLIQAKGGQYAESLTRYAGALNCRVGRKC 316
W W+S+ D+ LNGAFF SG Y R + AK ++T AGAL+C G C
Sbjct: 193 AWNWRSEGDMLLNGAFFTPSGAGASASYSRASSLGAKSSSMVATITSGAGALSCHKGSSC 372
Query: 315 * 313
*
Sbjct: 373 * 375
>gb|BI959076.1|BI959076 HVSMEn0018A17f Hordeum vulgare rachis EST library HVcDNA0015 (normal)
Hordeum vulgare subsp. vulgare cDNA clone HVSMEn0018A17f,
mRNA sequence
Length = 632
Score = 101 bits (216), Expect(2) = 9e-028
Identities = 44/101 (43%), Positives = 57/101 (56%)
Frame = -3 / +3
Query: 1053 GAQITLQNVRNVILHNLXXXXXXXXXXXAIRDSQHHWGVRXXXXXXXXXXXXXXDIWIDH 874
GA T+Q V NVI+H L +R S H+G R +W+DH
Sbjct: 324 GACTTIQYVTNVIIHGLHIHDCRPTGNAMVRSSPSHYGWRTIADGDAVXIFGASHVWVDH 503
Query: 873 LSMSSCADGLVDAVDGSTAITVSNGHFTRHDHVMLFGASDA 751
S+S+CADGL+DA+ GSTAITVSN +FT H+ ML G D+
Sbjct: 504 CSLSNCADGLIDAIMGSTAITVSNNYFTHHNEGMLLGHRDS 626
Score = 88.6 bits (187), Expect(2) = 1e-023
Identities = 48/100 (48%), Positives = 54/100 (54%)
Frame = +2 / -2
Query: 755 SLAPNSITWSCLVKWPLETVMAVEPSTASTSPSAQLLMDRWSIQISLDPITETPSPSLSP 934
SL P SI VK L+TVMAVEP AS SPSAQL ++WS Q L P T SPS
Sbjct: 622 SL*PRSIPSLWWVK*LLDTVMAVEPMMASMSPSAQLEREQWSTQTWLAPKMXTASPSAMV 443
Query: 935 RTPQWCCESRIAPPPCAAASWTCRLWRITLRTFCSVICAP 1054
R P W E R P SW R W +TL T+ V+ AP
Sbjct: 442 RHP*WLGELRTMALPVGRQSWMWRPWMMTLVTYWMVVQAP 323
Score = 42.3 bits (86), Expect(2) = 9e-028
Identities = 18/30 (60%), Positives = 21/30 (70%)
Frame = -3 / +3
Query: 1128 VELCQELIVSSDKTIDGRGAQVHIVGAQIT 1039
+ L QELI++S KTIDGRGA VHI T
Sbjct: 246 ITLSQELIMNSFKTIDGRGANVHIANGACT 335
Score = 41.8 bits (85), Expect(2) = 1e-023
Identities = 16/20 (80%), Positives = 16/20 (80%)
Frame = +2 / -2
Query: 1058 MCTCAPRPSMVLSLLTMSSW 1117
MCT APRPSMVL L MSSW
Sbjct: 316 MCTLAPRPSMVLKLFMMSSW 257
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 436,458,627
Number of Sequences: 312970
Number of extensions: 7412040
Number of successful extensions: 97257
Number of sequences better than 1.0e-020: 10
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 13581999925
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)