TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440716.2.1
(774 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF257303.2|BF257303 HVSMEf0012H19f Hordeum vulgare seedl... 136 2e-031
gb|AJ462135.1|AJ462135 AJ462135 S00002 Hordeum vulgare subs... 127 7e-029
gb|BM816261.1|BM816261 HC113F04_SK.ab1 HC Hordeum vulgare s... 112 1e-025
gb|AV833284.1|AV833284 AV833284 K. Sato unpublished cDNA li... 116 1e-025
gb|BJ463253.1|BJ463253 BJ463253 K. Sato unpublished cDNA li... 116 1e-025
gb|BM816260.1|BM816260 HC113E04_SK.ab1 HC Hordeum vulgare s... 112 2e-024
gb|BQ768447.1|BQ768447 EBro08_SQ011_B19_R root, 3 week, dro... 107 6e-023
gb|CA018416.1|CA018416 HV08I24r HV Hordeum vulgare subsp. v... 107 6e-023
gb|AV927784.1|AV927784 AV927784 K. Sato unpublished cDNA li... 107 8e-023
gb|AV929760.1|AV929760 AV929760 K. Sato unpublished cDNA li... 107 8e-023
gb|CA013515.1|CA013515 HT08L06r HT Hordeum vulgare subsp. v... 106 1e-022
gb|BQ763939.1|BQ763939 EBro03_SQ005_G23_R root, 3 week, wat... 104 6e-022
gb|BF260942.2|BF260942 HVSMEf0023F14f Hordeum vulgare seedl... 104 8e-022
gb|BQ660136.1|BQ660136 HI01C06w HI Hordeum vulgare subsp. v... 103 1e-021
gb|BF622731.2|BF622731 HVSMEa0008E02f Hordeum vulgare seedl... 100 1e-020
>gb|BF257303.2|BF257303 HVSMEf0012H19f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0012H19f, mRNA sequence
Length = 847
Score = 136 bits (291), Expect = 2e-031
Identities = 57/95 (60%), Positives = 62/95 (65%)
Frame = -2 / +3
Query: 773 PLRVVVRPNLIRGFESGALPDGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNST 594
P VVRP L+ LPDG E GRG+VV WAPQEEVL HPAV GF TH GWNST
Sbjct: 291 PFLWVVRPGLVAADGLTRLPDGFEAATSGRGMVVEWAPQEEVLRHPAVAGFWTHGGWNST 470
Query: 593 VEAISEGVPMVCCPRHGDQFGNMRYVCDVWKVGTE 489
E++ EGVPM+C P DQ GN R V VWKVG E
Sbjct: 471 TESVCEGVPMLCRPHLXDQMGNARDVKHVWKVGLE 575
>gb|AJ462135.1|AJ462135 AJ462135 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200058E12F1, mRNA sequence
Length = 360
Score = 127 bits (272), Expect = 7e-029
Identities = 49/70 (70%), Positives = 54/70 (77%)
Frame = -2 / +2
Query: 689 GRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAISEGVPMVCCPRHGDQFGNMRYVCD 510
GRG+VV WAPQEEVL HPAV GF TH GWNST E++ EGVPM+C P GDQ GN RYV
Sbjct: 8 GRGMVVEWAPQEEVLRHPAVAGFWTHGGWNSTTESMCEGVPMLCRPHFGDQMGNARYVEH 187
Query: 509 VWKVGTELVG 480
VWKVG E+ G
Sbjct: 188 VWKVGFEVAG 217
Score = 100 bits (214), Expect = 7e-021
Identities = 41/65 (63%), Positives = 47/65 (72%)
Frame = +1 / -1
Query: 472 SCSPTSSVPTFHTSHTYLMLPNWSPCRGQQTMGTPSEMASTVEFQPLWVRKPPTAGCAST 651
S +P +S PTFHT TYL P WSP G+Q++GTPS M S VEFQP V+ P TAGC ST
Sbjct: 225 SSAPATSKPTFHTCSTYLAFPIWSPKCGRQSIGTPSHMLSVVEFQPPCVQNPATAGCRST 46
Query: 652 SSCGA 666
SSCGA
Sbjct: 45 SSCGA 31
>gb|BM816261.1|BM816261 HC113F04_SK.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC113F04_SK.ab1 similar to
Galactosylgalactosylxylosylprotein
3-beta-glucuronosyltransferase, mRNA sequence
Length = 935
Score = 112 bits (240), Expect(2) = 1e-025
Identities = 43/91 (47%), Positives = 58/91 (63%)
Frame = -2 / +3
Query: 758 VRPNLIRGFESGALPDGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAIS 579
VRP+L++G ++ LP R ++ TW PQE+VL H A+G FLTH+GWNS++E I
Sbjct: 33 VRPDLVKGHDNAVLPPEFSAATEDRSMLSTWCPQEKVLEHEAIGVFLTHSGWNSSLEGIC 212
Query: 578 EGVPMVCCPRHGDQFGNMRYVCDVWKVGTEL 486
GVPMVC P +Q N RY C W +G E+
Sbjct: 213 GGVPMVCWPFFTEQQTNCRYKCTEWGIGMEI 305
Score = 23.5 bits (45), Expect(2) = 1e-025
Identities = 8/30 (26%), Positives = 19/30 (63%)
Frame = -2 / +3
Query: 485 VGEQLERGQVKAAIDRLFGTKEGEEIKERM 396
+GE++ R +V+A + +G+E++ R+
Sbjct: 303 IGEEVTRTEVEAILREAMEGGKGQEMRRRV 392
>gb|AV833284.1|AV833284 AV833284 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare shoots germination Hordeum vulgare subsp.
vulgare cDNA clone bags5b03, mRNA sequence
Length = 671
Score = 116 bits (248), Expect = 1e-025
Identities = 46/91 (50%), Positives = 58/91 (63%)
Frame = -2 / +1
Query: 758 VRPNLIRGFESGALPDGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAIS 579
VRP+L++G + ALP GR ++ TW PQE+VL AVG FLTH+GWNS++E I
Sbjct: 238 VRPDLVKGDDEAALPPEFSTATEGRSMLSTWCPQEKVLEQEAVGVFLTHSGWNSSLEGIC 417
Query: 578 EGVPMVCCPRHGDQFGNMRYVCDVWKVGTEL 486
GVPMVC P DQ N RY C W +G E+
Sbjct: 418 GGVPMVCWPFFADQQTNCRYKCTEWGIGMEI 510
>gb|BJ463253.1|BJ463253 BJ463253 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags26i01 5', mRNA sequence
Length = 518
Score = 116 bits (248), Expect = 1e-025
Identities = 46/91 (50%), Positives = 58/91 (63%)
Frame = -2 / +3
Query: 758 VRPNLIRGFESGALPDGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAIS 579
VRP+L++G + ALP GR ++ TW PQE+VL AVG FLTH+GWNS++E I
Sbjct: 201 VRPDLVKGDDEAALPPEFSTATEGRSMLSTWCPQEKVLEQEAVGVFLTHSGWNSSLEGIC 380
Query: 578 EGVPMVCCPRHGDQFGNMRYVCDVWKVGTEL 486
GVPMVC P DQ N RY C W +G E+
Sbjct: 381 GGVPMVCWPFFADQQTNCRYKCTEWGIGMEI 473
>gb|BM816260.1|BM816260 HC113E04_SK.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC113E04_SK.ab1 similar to
Galactosylgalactosylxylosylprotein
3-beta-glucuronosyltransferase, mRNA sequence
Length = 842
Score = 112 bits (240), Expect = 2e-024
Identities = 43/91 (47%), Positives = 58/91 (63%)
Frame = -2 / +2
Query: 758 VRPNLIRGFESGALPDGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAIS 579
VRP+L++G ++ LP R ++ TW PQE+VL H A+G FLTH+GWNS++E I
Sbjct: 206 VRPDLVKGHDNAVLPPEFSAATEDRSMLSTWCPQEKVLEHEAIGVFLTHSGWNSSLEGIC 385
Query: 578 EGVPMVCCPRHGDQFGNMRYVCDVWKVGTEL 486
GVPMVC P +Q N RY C W +G E+
Sbjct: 386 GGVPMVCWPFFTEQQTNCRYKCTEWGIGMEI 478
>gb|BQ768447.1|BQ768447 EBro08_SQ011_B19_R root, 3 week, drought-stressed, cv Optic, EBro08
Hordeum vulgare subsp. vulgare cDNA clone
EBro08_SQ011_B19 5', mRNA sequence
Length = 418
Score = 107 bits (229), Expect = 6e-023
Identities = 46/92 (50%), Positives = 60/92 (65%)
Frame = -2 / +3
Query: 761 VVRPNLIRGFESGALPDGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAI 582
V+RP++I +S L + V +G+ VV WAPQ +VL H AVG FLTH GWNST+E I
Sbjct: 33 VLRPDMIGASQSAVLQEAVGAAGKGKARVVGWAPQRDVLRHRAVGCFLTHAGWNSTLEGI 212
Query: 581 SEGVPMVCCPRHGDQFGNMRYVCDVWKVGTEL 486
+EGVP+VC P DQ N R+V VW G ++
Sbjct: 213 AEGVPLVCWPFFLDQQINSRFVGAVWGAGLDM 308
>gb|CA018416.1|CA018416 HV08I24r HV Hordeum vulgare subsp. vulgare cDNA clone HV08I24
5-PRIME, mRNA sequence
Length = 647
Score = 107 bits (229), Expect = 6e-023
Identities = 40/82 (48%), Positives = 55/82 (67%)
Frame = -2 / +3
Query: 731 ESGALPDGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAISEGVPMVCCP 552
++ LP+ E GRG++ +W PQ+EVL HPAVG FLTH+GWNS +E++ GVP++ P
Sbjct: 63 DAAVLPEEFLAETAGRGLMASWCPQQEVLNHPAVGAFLTHSGWNSALESLFGGVPVISWP 242
Query: 551 RHGDQFGNMRYVCDVWKVGTEL 486
DQ N RY C+ W VG E+
Sbjct: 243 FFADQQTNCRYQCNEWGVGMEI 308
>gb|AV927784.1|AV927784 AV927784 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd3k12 3', mRNA sequence
Length = 651
Score = 107 bits (228), Expect = 8e-023
Identities = 37/80 (46%), Positives = 54/80 (67%)
Frame = -2 / -1
Query: 713 DGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAISEGVPMVCCPRHGDQF 534
D + V G+G+VV WAPQ+ VL+HP+V F++H GWNST+E + GVP +C P DQF
Sbjct: 387 DAFKRRVEGKGLVVGWAPQQRVLSHPSVACFVSHCGWNSTMEGLRHGVPFLCWPYFADQF 208
Query: 533 GNMRYVCDVWKVGTELVGEQ 474
N Y+C+VW G ++ ++
Sbjct: 207 CNQSYICNVWGTGVKIHADE 148
>gb|AV929760.1|AV929760 AV929760 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd16h10 3', mRNA sequence
Length = 669
Score = 107 bits (228), Expect = 8e-023
Identities = 37/80 (46%), Positives = 54/80 (67%)
Frame = -2 / -3
Query: 713 DGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAISEGVPMVCCPRHGDQF 534
D + V G+G+VV WAPQ+ VL+HP+V F++H GWNST+E + GVP +C P DQF
Sbjct: 400 DAFKRRVEGKGLVVGWAPQQRVLSHPSVACFVSHCGWNSTMEGLRHGVPFLCWPYFADQF 221
Query: 533 GNMRYVCDVWKVGTELVGEQ 474
N Y+C+VW G ++ ++
Sbjct: 220 CNQSYICNVWGTGVKIHADE 161
>gb|CA013515.1|CA013515 HT08L06r HT Hordeum vulgare subsp. vulgare cDNA clone HT08L06
5-PRIME, mRNA sequence
Length = 634
Score = 106 bits (227), Expect = 1e-022
Identities = 36/80 (45%), Positives = 54/80 (67%)
Frame = -2 / +1
Query: 713 DGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAISEGVPMVCCPRHGDQF 534
D + V G+G+VV WAPQ+ VL+HP++ F++H GWNST+E + GVP +C P DQF
Sbjct: 226 DAFKRRVEGKGLVVGWAPQQRVLSHPSIACFVSHCGWNSTMEGLRHGVPFLCWPYFADQF 405
Query: 533 GNMRYVCDVWKVGTELVGEQ 474
N Y+C+VW G ++ ++
Sbjct: 406 CNQSYICNVWGTGVKIHADE 465
>gb|BQ763939.1|BQ763939 EBro03_SQ005_G23_R root, 3 week, waterlogged, cv Optic, EBro03
Hordeum vulgare subsp. vulgare cDNA clone
EBro03_SQ005_G23 5', mRNA sequence
Length = 594
Score = 104 bits (222), Expect = 6e-022
Identities = 43/79 (54%), Positives = 51/79 (64%)
Frame = -2 / +2
Query: 722 ALPDGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAISEGVPMVCCPRHG 543
ALP G + RGRG+V +W QE VL H AV FLTH+GWNSTVEA+ GVPM+C P
Sbjct: 188 ALPPGFLEATRGRGLVASWCDQEAVLRHDAVCVFLTHSGWNSTVEALCGGVPMLCWPFFA 367
Query: 542 DQFGNMRYVCDVWKVGTEL 486
+Q N RY C W V E+
Sbjct: 368 EQQTNCRYKCVEWGVAMEI 424
>gb|BF260942.2|BF260942 HVSMEf0023F14f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0023F14f, mRNA sequence
Length = 816
Score = 104 bits (221), Expect = 8e-022
Identities = 42/96 (43%), Positives = 60/96 (62%)
Frame = -2 / +3
Query: 773 PLRVVVRPNLIRGFESGALPDGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNST 594
P V+RP+++ ++ L D + + + VV WAPQ +VL H AVG FLTH GWNST
Sbjct: 108 PFLWVLRPDMVGASQNAVLQDAIRTAGKSKARVVEWAPQRDVLRHRAVGCFLTHAGWNST 287
Query: 593 VEAISEGVPMVCCPRHGDQFGNMRYVCDVWKVGTEL 486
+E+++EGVP VC P DQ N R++ VW G ++
Sbjct: 288 LESVAEGVPTVCWPFFVDQQINSRFMGAVWGSGLDM 395
>gb|BQ660136.1|BQ660136 HI01C06w HI Hordeum vulgare subsp. vulgare cDNA clone HI01C06
3-PRIME, mRNA sequence
Length = 668
Score = 103 bits (220), Expect = 1e-021
Identities = 42/78 (53%), Positives = 51/78 (65%)
Frame = -2 / -3
Query: 719 LPDGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAISEGVPMVCCPRHGD 540
LP+G GRG+VV W Q EVLAH A+GGFLTH GWNS +E++ GVPM+C P D
Sbjct: 663 LPEGFAAACAGRGLVVPWCCQVEVLAHAALGGFLTHCGWNSVLESVWAGVPMLCFPLLTD 484
Query: 539 QFGNMRYVCDVWKVGTEL 486
QF N R V W+VG +
Sbjct: 483 QFTNRRLVVREWRVGVPI 430
>gb|BF622731.2|BF622731 HVSMEa0008E02f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0008E02f, mRNA sequence
Length = 718
Score = 100 bits (213), Expect = 1e-020
Identities = 38/82 (46%), Positives = 53/82 (64%)
Frame = -2 / +2
Query: 731 ESGALPDGVEDEVRGRGIVVTWAPQEEVLAHPAVGGFLTHNGWNSTVEAISEGVPMVCCP 552
++ LP+ E GRG++ +W PQ+EVL HPAVG FLTH+GWNS +E++ GVP++ P
Sbjct: 413 DAAVLPEEFLAETAGRGLMASWCPQQEVLNHPAVGAFLTHSGWNSALESLFGGVPVISWP 592
Query: 551 RHGDQFGNMRYVCDVWKVGTEL 486
DQ N R C+ W G E+
Sbjct: 593 FFADQQTNCRSQCNEWGRGMEI 658
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 318,859,651
Number of Sequences: 312970
Number of extensions: 4423641
Number of successful extensions: 60562
Number of sequences better than 1.0e-020: 22
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 8631266495
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)