TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2440419.2.1
(616 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BG344802.2|BG344802 HVSMEg0016C08f Hordeum vulgare pre-a... 285 1e-076
gb|BF260181.3|BF260181 HVSMEf0021F04f Hordeum vulgare seedl... 248 1e-065
gb|CA026428.1|CA026428 HZ55O06r HZ Hordeum vulgare subsp. v... 235 1e-061
gb|CA030032.1|CA030032 HX05O06r HX Hordeum vulgare subsp. v... 117 3e-047
gb|CA031878.1|CA031878 HX11G06r HX Hordeum vulgare subsp. v... 120 4e-045
gb|BF259968.2|BF259968 HVSMEf0020M01f Hordeum vulgare seedl... 109 1e-044
gb|CB859738.1|CB859738 HI11H04w HI Hordeum vulgare subsp. v... 171 3e-042
gb|CA020103.1|CA020103 HV14F14r HV Hordeum vulgare subsp. v... 99 1e-041
gb|BI956646.1|BI956646 HVSMEn0004J02f Hordeum vulgare rachi... 158 8e-041
gb|AL505263.1|AL505263 AL505263 Hordeum vulgare Barke roots... 117 3e-037
gb|BI959125.1|BI959125 HVSMEn0018E15f Hordeum vulgare rachi... 140 7e-033
gb|BI779064.2|BI779064 EBro01_SQ002_M23_R root, 3 week, hyd... 118 3e-026
gb|AL502543.1|AL502543 AL502543 Hordeum vulgare Barke roots... 117 4e-026
>gb|BG344802.2|BG344802 HVSMEg0016C08f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0016C08f, mRNA sequence
Length = 794
Score = 285 bits (617), Expect = 1e-076
Identities = 116/158 (73%), Positives = 121/158 (76%)
Frame = -3 / +2
Query: 581 ETFSREYDVTLPPPNPNTTAGDNVYVLAHNTTVDVLLQNANALSRNVSEVHPWHLHGHDF 402
+TF R YDVT P NPNTT GDNVYVL HNTTVDV+LQNANAL NVSEVHPWHLHGHDF
Sbjct: 17 DTFGRAYDVTRPTHNPNTTTGDNVYVLRHNTTVDVVLQNANALQHNVSEVHPWHLHGHDF 196
Query: 401 WVLXXXXXXXXXXXXXXXRLNLRDPPLRNTAVIFPYGWTMLRFVADNPGVWAFHCHIEPH 222
WVL RLNL +PPLRNTAVIFPYGWT LRFVADNPGVWAFH HIEPH
Sbjct: 197 WVLGYGEGAYRGDAADAARLNLVNPPLRNTAVIFPYGWTALRFVADNPGVWAFH*HIEPH 376
Query: 221 LHMGMGVIFAEAVDLVAKVPNEAVSCGAXATALMAGGH 108
LHMGMGVIFAE ++ V KVP EAV C A ATALM H
Sbjct: 377 LHMGMGVIFAEVIERVGKVPKEAVFCSATATALMNCDH 490
Score = 219 bits (472), Expect = 1e-056
Identities = 100/151 (66%), Positives = 104/151 (68%)
Frame = +1 / -3
Query: 145 QDTASLGTLATRSTASAKMTPMPMWRCGSIWQWKAHTPGLSATNRSIVHPYGKMTAVFRS 324
Q TASLGT TRS SAKMTPMPMWRCGS+ QW AHTPGLSATNRS VHPYGK+TAVFRS
Sbjct: 453 QKTASLGTFPTRSMTSAKMTPMPMWRCGSMCQWNAHTPGLSATNRSAVHPYGKITAVFRS 274
Query: 325 GGXXXXXXXXXXXXXXXXXXXXXXTQKSWPWRCHGCTSLTLRDSALAFWSSTSTVVLCAR 504
GG TQKS P RCHGCTSLTL SALAF S+TSTVVL
Sbjct: 273 GGFTRFSLAASAASPLYAPSPYPSTQKSCPCRCHGCTSLTLCCSALAF*STTSTVVLWRS 94
Query: 505 TYTLSPAVVLGFGGGSVTSYSRLNVSAXRAA 597
TYTLSP VV G G VTS +R NVSA RAA
Sbjct: 93 TYTLSPVVVFGLCVGRVTS*ARPNVSAGRAA 1
Score = 90.0 bits (190), Expect(3) = 1e-036
Identities = 37/61 (60%), Positives = 44/61 (72%)
Frame = +3 / -2
Query: 99 QSQVTAGHERGGRGATGYSLVGHLGDEVDSLSEDDAHAHVEVRLDMAVEGPHARVVRHEP 278
+S V HERGGR A L+GHL D D L +DD HAHVEVRLD++VE PHA VVRH+P
Sbjct: 499 RSGVITVHERGGRSAAENGLLGHLPDAFDDLGKDDPHAHVEVRLDVSVERPHAGVVRHKP 320
Query: 279 E 281
+
Sbjct: 319 Q 317
Score = 61.6 bits (128), Expect(3) = 1e-036
Identities = 26/37 (70%), Positives = 29/37 (78%)
Frame = +3 / -2
Query: 393 QHPEVVAVEVPRVHLADVARQRVGVLEQHVDGGVVRQ 503
QHPEVV V+VPRVHLA V QRVGVL+ HV GVV +
Sbjct: 205 QHPEVVPVQVPRVHLAHVVLQRVGVLKHHVHRGVVAE 95
Score = 39.6 bits (80), Expect(3) = 1e-036
Identities = 15/19 (78%), Positives = 16/19 (84%)
Frame = +2 / -1
Query: 506 RTRCPPPSCWGSAAAASRR 562
RTRCPP SC G A+AASRR
Sbjct: 92 RTRCPPSSCLGCASAASRR 36
>gb|BF260181.3|BF260181 HVSMEf0021F04f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0021F04f, mRNA sequence
Length = 650
Score = 248 bits (537), Expect = 1e-065
Identities = 99/130 (76%), Positives = 103/130 (79%)
Frame = -3 / +2
Query: 497 HNTTVDVLLQNANALSRNVSEVHPWHLHGHDFWVLXXXXXXXXXXXXXXXRLNLRDPPLR 318
HNTTVDV+LQNANAL NVSEVHPWHLHGHDFWVL RLNL +PPLR
Sbjct: 2 HNTTVDVVLQNANALQHNVSEVHPWHLHGHDFWVLNNGEGAYRGDAADAARLNLVNPPLR 181
Query: 317 NTAVIFPYGWTMLRFVADNPGVWAFHCHIEPHLHMGMGVIFAEAVDLVAKVPNEAVSCGA 138
NTA IFPYGWT LRFVADNPGVWAFHCHIEPHLHMGMGVIFAEA++ V KVP EAV CGA
Sbjct: 182 NTAEIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVIFAEAIERVGKVPKEAVFCGA 361
Query: 137 XATALMAGGH 108
ATALM G H
Sbjct: 362 TATALMNGDH 391
Score = 193 bits (415), Expect = 1e-048
Identities = 80/117 (68%), Positives = 85/117 (72%)
Frame = +1 / -3
Query: 145 QDTASLGTLATRSTASAKMTPMPMWRCGSIWQWKAHTPGLSATNRSIVHPYGKMTAVFRS 324
Q TASLGT TRS ASAKMTPMPMWRCGS+WQW AHTPGLSATNRS VHPYGK++AVFR+
Sbjct: 354 QKTASLGTFPTRSMASAKMTPMPMWRCGSMWQWNAHTPGLSATNRSAVHPYGKISAVFRN 175
Query: 325 GGXXXXXXXXXXXXXXXXXXXXXXTQKSWPWRCHGCTSLTLRDSALAFWSSTSTVVL 495
GG TQKS P RCHGCTSLTL SALAFWS+TSTVVL
Sbjct: 174 GGFTRFSLAASAASPLYAPSPLFSTQKSCPCRCHGCTSLTLCCSALAFWSTTSTVVL 4
Score = 96.8 bits (205), Expect(2) = 5e-032
Identities = 41/61 (67%), Positives = 45/61 (73%)
Frame = +3 / -2
Query: 99 QSQVTAGHERGGRGATGYSLVGHLGDEVDSLSEDDAHAHVEVRLDMAVEGPHARVVRHEP 278
+S V A HERGGRGA L+GHL D D L EDD HAHVEVRLD+AVE PH VVRHEP
Sbjct: 400 RSGVIAVHERGGRGAAEDGLLGHLPDAFDGLGEDDPHAHVEVRLDVAVERPHPGVVRHEP 221
Query: 279 E 281
+
Sbjct: 220 Q 218
Score = 60.6 bits (126), Expect(2) = 5e-032
Identities = 26/34 (76%), Positives = 27/34 (79%)
Frame = +3 / -2
Query: 396 HPEVVAVEVPRVHLADVARQRVGVLEQHVDGGVV 497
HPEVV V+VPRVHLA V QRVGVLE HV GVV
Sbjct: 103 HPEVVPVQVPRVHLAHVVLQRVGVLEHHVHRGVV 2
>gb|CA026428.1|CA026428 HZ55O06r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ55O06
5-PRIME, mRNA sequence
Length = 479
Score = 235 bits (508), Expect = 1e-061
Identities = 94/123 (76%), Positives = 98/123 (79%)
Frame = -3 / +1
Query: 470 QNANALSRNVSEVHPWHLHGHDFWVLXXXXXXXXXXXXXXXRLNLRDPPLRNTAVIFPYG 291
+NANAL NVSEVHPWHLHGHDFWVL RLNL +PPLRNTAVIFPYG
Sbjct: 10 ENANALQHNVSEVHPWHLHGHDFWVLGYGEGAYRGDAADAARLNLVNPPLRNTAVIFPYG 189
Query: 290 WTMLRFVADNPGVWAFHCHIEPHLHMGMGVIFAEAVDLVAKVPNEAVSCGAXATALMAGG 111
WT LRFVADNPGVWAFHCHIEPHLHMGMGVIFAEA++ V KVP EAVSCGA ATALM G
Sbjct: 190 WTALRFVADNPGVWAFHCHIEPHLHMGMGVIFAEAIERVGKVPKEAVSCGATATALMNGD 369
Query: 110 HL* 102
HL*
Sbjct: 370 HL* 378
Score = 178 bits (383), Expect = 3e-044
Identities = 75/110 (68%), Positives = 78/110 (70%)
Frame = +1 / -1
Query: 145 QDTASLGTLATRSTASAKMTPMPMWRCGSIWQWKAHTPGLSATNRSIVHPYGKMTAVFRS 324
Q+TASLGT TRS ASAKMTPMPMWRCGS+WQW AHTPGLSATNRS VHPYGK+TAVFRS
Sbjct: 335 QETASLGTFPTRSMASAKMTPMPMWRCGSMWQWNAHTPGLSATNRSAVHPYGKITAVFRS 156
Query: 325 GGXXXXXXXXXXXXXXXXXXXXXXTQKSWPWRCHGCTSLTLRDSALAFWS 474
GG TQKS P RCHGCTSLTL SALAF S
Sbjct: 155 GGFTRFSLAASAASPLYAPSPYPSTQKSCPCRCHGCTSLTLCCSALAFSS 6
Score = 102 bits (217), Expect(2) = 1e-030
Identities = 42/61 (68%), Positives = 47/61 (77%)
Frame = +3 / -3
Query: 99 QSQVTAGHERGGRGATGYSLVGHLGDEVDSLSEDDAHAHVEVRLDMAVEGPHARVVRHEP 278
+S+V A HERGGRGA G L+GHL D D L EDD HAHVEVRLD+AVE PH VVRHEP
Sbjct: 381 RSEVIAVHERGGRGAAGDGLLGHLPDAFDGLGEDDPHAHVEVRLDVAVERPHPGVVRHEP 202
Query: 279 E 281
+
Sbjct: 201 Q 199
Score = 50.1 bits (103), Expect(2) = 1e-030
Identities = 21/26 (80%), Positives = 22/26 (84%)
Frame = +3 / -3
Query: 393 QHPEVVAVEVPRVHLADVARQRVGVL 470
QHPEVV V+VPRVHLA V QRVGVL
Sbjct: 87 QHPEVVPVQVPRVHLAHVVLQRVGVL 10
>gb|CA030032.1|CA030032 HX05O06r HX Hordeum vulgare subsp. vulgare cDNA clone HX05O06
5-PRIME, mRNA sequence
Length = 654
Score = 117 bits (251), Expect(2) = 3e-047
Identities = 42/66 (63%), Positives = 48/66 (72%)
Frame = -3 / +1
Query: 341 NLRDPPLRNTAVIFPYGWTMLRFVADNPGVWAFHCHIEPHLHMGMGVIFAEAVDLVAKVP 162
NLRDP ++NT + PYGWT LRF ADNPGVWAFHCHIE H MGMGV F E ++ V K+P
Sbjct: 274 NLRDPVMKNTVAVHPYGWTALRFRADNPGVWAFHCHIEAHFFMGMGVAFEEGIERVGKLP 453
Query: 161 NEAVSC 144
E C
Sbjct: 454 EEITRC 471
Score = 90.4 bits (191), Expect(2) = 3e-047
Identities = 35/57 (61%), Positives = 39/57 (68%)
Frame = -3 / +1
Query: 563 YDVTLPPPNPNTTAGDNVYVLAHNTTVDVLLQNANALSRNVSEVHPWHLHGHDFWVL 393
YDV NPN T D +Y LA + VDV+LQNAN L+ N SE HPWHLHGHDFW L
Sbjct: 55 YDVYGVQANPNATTSDGLYRLAFGSVVDVVLQNANMLAPNNSETHPWHLHGHDFWTL 225
Score = 83.1 bits (175), Expect(2) = 9e-029
Identities = 35/58 (60%), Positives = 39/58 (67%)
Frame = +1 / -2
Query: 145 QDTASLGTLATRSTASAKMTPMPMWRCGSIWQWKAHTPGLSATNRSIVHPYGKMTAVF 318
Q S G+L TRS S+K TPMPM +C S+WQWKA TPGLSA NRS V P G VF
Sbjct: 470 QRVISSGSLPTRSMPSSKATPMPMKKCASMWQWKAQTPGLSARNRSAVQP*GCTATVF 297
Score = 63.4 bits (132), Expect(2) = 9e-029
Identities = 30/54 (55%), Positives = 32/54 (59%)
Frame = +1 / -2
Query: 400 QKSWPWRCHGCTSLTLRDSALAFWSSTSTVVLCARTYTLSPAVVLGFGGGSVTS 561
QKSWPWRCHG SL L S LAF S+TST A Y+ S V LGF TS
Sbjct: 218 QKSWPWRCHGWVSLLLGASMLAFCSTTSTTEPNASRYSPSLVVALGFACTPYTS 57
>gb|CA031878.1|CA031878 HX11G06r HX Hordeum vulgare subsp. vulgare cDNA clone HX11G06
5-PRIME, mRNA sequence
Length = 624
Score = 120 bits (257), Expect(2) = 4e-045
Identities = 42/55 (76%), Positives = 46/55 (83%)
Frame = -3 / +2
Query: 344 LNLRDPPLRNTAVIFPYGWTMLRFVADNPGVWAFHCHIEPHLHMGMGVIFAEAVD 180
LN DPPLRNT V+FP+GWT +RFVADN G WAFHCHIEPHLHMGMG +F E VD
Sbjct: 383 LNTEDPPLRNTVVVFPHGWTAIRFVADNVGAWAFHCHIEPHLHMGMGAVFVEGVD 547
Score = 90.9 bits (192), Expect(2) = 5e-033
Identities = 34/50 (68%), Positives = 36/50 (72%)
Frame = +1 / -1
Query: 181 STASAKMTPMPMWRCGSIWQWKAHTPGLSATNRSIVHPYGKMTAVFRSGG 330
ST S K P+PMWRCGS+WQW AH P LSATNR VHP G T VF SGG
Sbjct: 546 STPSTKTAPIPMWRCGSMWQWNAHAPTLSATNRIAVHPCGNTTTVFLSGG 397
Score = 80.8 bits (170), Expect(2) = 4e-045
Identities = 30/46 (65%), Positives = 32/46 (69%)
Frame = -3 / +2
Query: 530 TTAGDNVYVLAHNTTVDVLLQNANALSRNVSEVHPWHLHGHDFWVL 393
T D VY L H VDV+LQNA+ L N SE HPWHLHGHDFWVL
Sbjct: 212 TRLSDRVYELPHGAVVDVVLQNADMLRHNESETHPWHLHGHDFWVL 349
Score = 69.8 bits (146), Expect(2) = 5e-033
Identities = 28/46 (60%), Positives = 33/46 (71%)
Frame = +1 / -1
Query: 397 TQKSWPWRCHGCTSLTLRDSALAFWSSTSTVVLCARTYTLSPAVVL 534
TQKSWP RCHGC SL+L S AFWS+TST C +YT S ++VL
Sbjct: 345 TQKSWPCRCHGCVSLSLCLSMSAFWSTTSTTAPCGSSYTRSLSLVL 208
>gb|BF259968.2|BF259968 HVSMEf0020M01f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020M01f, mRNA sequence
Length = 536
Score = 109 bits (232), Expect(2) = 1e-044
Identities = 39/59 (66%), Positives = 45/59 (76%)
Frame = -3 / +1
Query: 341 NLRDPPLRNTAVIFPYGWTMLRFVADNPGVWAFHCHIEPHLHMGMGVIFAEAVDLVAKV 165
NLRDP ++NT + PYGWT LRF ADNPGVWAFHCHIE H MGMGV F E ++ V K+
Sbjct: 358 NLRDPVMKNTVAVHPYGWTALRFRADNPGVWAFHCHIEAHFFMGMGVAFEEGIERVGKL 534
Score = 90.4 bits (191), Expect(2) = 1e-044
Identities = 35/57 (61%), Positives = 39/57 (68%)
Frame = -3 / +1
Query: 563 YDVTLPPPNPNTTAGDNVYVLAHNTTVDVLLQNANALSRNVSEVHPWHLHGHDFWVL 393
YDV NPN T D +Y LA + VDV+LQNAN L+ N SE HPWHLHGHDFW L
Sbjct: 139 YDVYGVQANPNATTSDGLYRLAFGSVVDVVLQNANMLAPNNSETHPWHLHGHDFWTL 309
Score = 78.5 bits (165), Expect(2) = 2e-027
Identities = 32/51 (62%), Positives = 36/51 (70%)
Frame = +1 / -1
Query: 166 TLATRSTASAKMTPMPMWRCGSIWQWKAHTPGLSATNRSIVHPYGKMTAVF 318
+L TRS S+K TPMPM +C S+WQWKA TPGLSA NRS V P G VF
Sbjct: 533 SLPTRSMPSSKATPMPMKKCASMWQWKAQTPGLSARNRSAVQP*GCTATVF 381
Score = 63.4 bits (132), Expect(2) = 2e-027
Identities = 30/54 (55%), Positives = 32/54 (59%)
Frame = +1 / -1
Query: 400 QKSWPWRCHGCTSLTLRDSALAFWSSTSTVVLCARTYTLSPAVVLGFGGGSVTS 561
QKSWPWRCHG SL L S LAF S+TST A Y+ S V LGF TS
Sbjct: 302 QKSWPWRCHGWVSLLLGASMLAFCSTTSTTEPNASRYSPSLVVALGFACTPYTS 141
>gb|CB859738.1|CB859738 HI11H04w HI Hordeum vulgare subsp. vulgare cDNA clone HI11H04
3-PRIME, mRNA sequence
Length = 514
Score = 171 bits (368), Expect = 3e-042
Identities = 67/77 (87%), Positives = 70/77 (90%)
Frame = -3 / -1
Query: 332 DPPLRNTAVIFPYGWTMLRFVADNPGVWAFHCHIEPHLHMGMGVIFAEAVDLVAKVPNEA 153
+PPLRNTAVIFPYGWT LRFVADNPGVWAFHCHIEPHLHMGMGVIFAEA++ V KVP EA
Sbjct: 466 NPPLRNTAVIFPYGWTALRFVADNPGVWAFHCHIEPHLHMGMGVIFAEAIERVGKVPKEA 287
Query: 152 VSCGAXATALMAGGHL* 102
VSCGA ATALM G HL*
Sbjct: 286 VSCGATATALMNGDHL* 236
Score = 140 bits (300), Expect = 7e-033
Identities = 54/62 (87%), Positives = 57/62 (91%)
Frame = +1 / +3
Query: 145 QDTASLGTLATRSTASAKMTPMPMWRCGSIWQWKAHTPGLSATNRSIVHPYGKMTAVFRS 324
Q+TASLGT TRS ASAKMTPMPMWRCGS+WQW AHTPGLSATNRS VHPYGK+TAVFRS
Sbjct: 279 QETASLGTFPTRSMASAKMTPMPMWRCGSMWQWNAHTPGLSATNRSAVHPYGKITAVFRS 458
Query: 325 GG 330
GG
Sbjct: 459 GG 464
Score = 102 bits (217), Expect = 2e-021
Identities = 42/61 (68%), Positives = 47/61 (77%)
Frame = +3 / +2
Query: 99 QSQVTAGHERGGRGATGYSLVGHLGDEVDSLSEDDAHAHVEVRLDMAVEGPHARVVRHEP 278
+S+V A HERGGRGA G L+GHL D D L EDD HAHVEVRLD+AVE PH VVRHEP
Sbjct: 233 RSEVIAVHERGGRGAAGDGLLGHLPDAFDGLGEDDPHAHVEVRLDVAVERPHPGVVRHEP 412
Query: 279 E 281
+
Sbjct: 413 Q 415
>gb|CA020103.1|CA020103 HV14F14r HV Hordeum vulgare subsp. vulgare cDNA clone HV14F14
5-PRIME, mRNA sequence
Length = 504
Score = 99.1 bits (210), Expect(2) = 1e-041
Identities = 35/47 (74%), Positives = 38/47 (80%)
Frame = -3 / +2
Query: 341 NLRDPPLRNTAVIFPYGWTMLRFVADNPGVWAFHCHIEPHLHMGMGV 201
NLRDP ++NT + PYGWT LRF ADNPGVWAFHCHIE H MGMGV
Sbjct: 359 NLRDPVMKNTVAVHPYGWTALRFRADNPGVWAFHCHIEAHFFMGMGV 499
Score = 90.4 bits (191), Expect(2) = 1e-041
Identities = 35/57 (61%), Positives = 39/57 (68%)
Frame = -3 / +2
Query: 563 YDVTLPPPNPNTTAGDNVYVLAHNTTVDVLLQNANALSRNVSEVHPWHLHGHDFWVL 393
YDV NPN T D +Y LA + VDV+LQNAN L+ N SE HPWHLHGHDFW L
Sbjct: 140 YDVYGVQANPNATTSDGLYRLAFGSVVDVVLQNANMLAPNNSETHPWHLHGHDFWTL 310
Score = 68.9 bits (144), Expect(2) = 1e-024
Identities = 27/41 (65%), Positives = 29/41 (70%)
Frame = +1 / -1
Query: 196 KMTPMPMWRCGSIWQWKAHTPGLSATNRSIVHPYGKMTAVF 318
K TPMPM +C S+WQWKA TPGLSA NRS V P G VF
Sbjct: 504 KATPMPMKKCASMWQWKAQTPGLSARNRSAVQP*GCTATVF 382
Score = 63.4 bits (132), Expect(2) = 1e-024
Identities = 30/54 (55%), Positives = 32/54 (59%)
Frame = +1 / -1
Query: 400 QKSWPWRCHGCTSLTLRDSALAFWSSTSTVVLCARTYTLSPAVVLGFGGGSVTS 561
QKSWPWRCHG SL L S LAF S+TST A Y+ S V LGF TS
Sbjct: 303 QKSWPWRCHGWVSLLLGASMLAFCSTTSTTEPNASRYSPSLVVALGFACTPYTS 142
>gb|BI956646.1|BI956646 HVSMEn0004J02f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0004J02f, mRNA sequence
Length = 810
Score = 158 bits (340), Expect(2) = 8e-041
Identities = 66/95 (69%), Positives = 69/95 (72%)
Frame = -3 / +1
Query: 581 ETFSREYDVTLPPPNPNTTAGDNVYVLAHNTTVDVLLQNANALSRNVSEVHPWHLHGHDF 402
+TF R YDVT PP NPNTT GDNVYVL HNTTVDV+LQNANAL NVSEVHPWHLHGHDF
Sbjct: 337 DTFGRAYDVTRPPHNPNTTTGDNVYVLRHNTTVDVVLQNANALQHNVSEVHPWHLHGHDF 516
Query: 401 WVLXXXXXXXXXXXXXXXRLNLRDPPLRNTAVIFP 297
WVL RL +PPLRN AVIFP
Sbjct: 517 WVLGYGEGGVRGDAXDAARLEPVNPPLRNXAVIFP 621
Score = 122 bits (262), Expect(2) = 6e-029
Identities = 60/100 (60%), Positives = 63/100 (63%)
Frame = +1 / -2
Query: 298 GKMTAVFRSGGXXXXXXXXXXXXXXXXXXXXXXTQKSWPWRCHGCTSLTLRDSALAFWSS 477
GK+TA FRSGG TQKS P RCHGCTSLTL SALAFWS+
Sbjct: 620 GKITAXFRSGGFTGSSLAASXASPLTPPSPYPSTQKSCPCRCHGCTSLTLCCSALAFWST 441
Query: 478 TSTVVLCARTYTLSPAVVLGFGGGSVTSYSRLNVSAXRAA 597
TSTVVL TYTLSP VV G GG VTS +R NVSA RAA
Sbjct: 440 TSTVVLWRSTYTLSPVVVFGLCGGRVTS*ARPNVSAGRAA 321
Score = 28.1 bits (55), Expect(2) = 8e-041
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = -3 / +3
Query: 293 GWTMLRFVADNPGVWA 246
G LRF ADNPG WA
Sbjct: 627 GGRRLRFGADNPGGWA 674
Score = 24.0 bits (46), Expect(2) = 6e-029
Identities = 9/11 (81%), Positives = 9/11 (81%)
Frame = +1 / -3
Query: 247 AHTPGLSATNR 279
AH PGLSA NR
Sbjct: 673 AHPPGLSAPNR 641
>gb|AL505263.1|AL505263 AL505263 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07O19V 5', mRNA sequence
Length = 612
Score = 117 bits (251), Expect(2) = 3e-037
Identities = 42/66 (63%), Positives = 48/66 (72%)
Frame = -3 / +2
Query: 341 NLRDPPLRNTAVIFPYGWTMLRFVADNPGVWAFHCHIEPHLHMGMGVIFAEAVDLVAKVP 162
NLRDP ++NT + PYGWT LRF ADNPGVWAFHCHIE H MGMGV F E ++ V K+P
Sbjct: 152 NLRDPVMKNTVAVHPYGWTALRFRADNPGVWAFHCHIEAHFFMGMGVAFEEGIERVGKLP 331
Query: 161 NEAVSC 144
E C
Sbjct: 332 EEITRC 349
Score = 83.1 bits (175), Expect(2) = 2e-023
Identities = 35/58 (60%), Positives = 39/58 (67%)
Frame = +1 / -1
Query: 145 QDTASLGTLATRSTASAKMTPMPMWRCGSIWQWKAHTPGLSATNRSIVHPYGKMTAVF 318
Q S G+L TRS S+K TPMPM +C S+WQWKA TPGLSA NRS V P G VF
Sbjct: 348 QRVISSGSLPTRSMPSSKATPMPMKKCASMWQWKAQTPGLSARNRSAVQP*GCTATVF 175
Score = 57.0 bits (118), Expect(2) = 3e-037
Identities = 19/26 (73%), Positives = 21/26 (80%)
Frame = -3 / +2
Query: 470 QNANALSRNVSEVHPWHLHGHDFWVL 393
+NAN L+ N SE HPWHLHGHDFW L
Sbjct: 26 KNANMLAPNNSETHPWHLHGHDFWTL 103
Score = 45.5 bits (93), Expect(2) = 2e-023
Identities = 17/23 (73%), Positives = 17/23 (73%)
Frame = +1 / -1
Query: 400 QKSWPWRCHGCTSLTLRDSALAF 468
QKSWPWRCHG SL L S LAF
Sbjct: 96 QKSWPWRCHGWVSLLLGASMLAF 28
>gb|BI959125.1|BI959125 HVSMEn0018E15f Hordeum vulgare rachis EST library HVcDNA0015
(normal) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEn0018E15f, mRNA sequence
Length = 656
Score = 140 bits (300), Expect = 7e-033
Identities = 53/63 (84%), Positives = 55/63 (87%)
Frame = -3 / +1
Query: 581 ETFSREYDVTLPPPNPNTTAGDNVYVLAHNTTVDVLLQNANALSRNVSEVHPWHLHGHDF 402
+TF R YDVT PP NPNTT GDNVYVL HNTTVDV+LQNANAL NVSEVHPWHLHGHDF
Sbjct: 337 DTFGRAYDVTRPPHNPNTTTGDNVYVLRHNTTVDVVLQNANALQHNVSEVHPWHLHGHDF 516
Query: 401 WVL 393
WVL
Sbjct: 517 WVL 525
Score = 113 bits (242), Expect = 7e-025
Identities = 51/67 (76%), Positives = 53/67 (79%)
Frame = +1 / -1
Query: 397 TQKSWPWRCHGCTSLTLRDSALAFWSSTSTVVLCARTYTLSPAVVLGFGGGSVTSYSRLN 576
TQKS P RCHGCTSLTL SALAFWS+TSTVVL TYTLSP VV G GG VTSY+R N
Sbjct: 521 TQKSCPCRCHGCTSLTLCCSALAFWSTTSTVVLWRSTYTLSPVVVFGLCGGRVTSYARPN 342
Query: 577 VSAXRAA 597
VSA RAA
Sbjct: 341 VSAGRAA 321
>gb|BI779064.2|BI779064 EBro01_SQ002_M23_R root, 3 week, hydroponic grown, no treatment, cv
Optic, EBro01 Hordeum vulgare subsp. vulgare cDNA clone
EBro01_SQ002_M23 5', mRNA sequence
Length = 414
Score = 118 bits (252), Expect = 3e-026
Identities = 43/89 (48%), Positives = 56/89 (62%)
Frame = -3 / +1
Query: 407 DFWVLXXXXXXXXXXXXXXXRLNLRDPPLRNTAVIFPYGWTMLRFVADNPGVWAFHCHIE 228
DFWVL LN+RDP ++NT + P GWT +RF ADNPGVW FHCH+E
Sbjct: 1 DFWVLGHGEGKFNPAADAWRLLNVRDPIMKNTVPLHPDGWTAVRFRADNPGVWLFHCHVE 180
Query: 227 PHLHMGMGVIFAEAVDLVAKVPNEAVSCG 141
H+ MGMGV+F E V+ V ++P+ + CG
Sbjct: 181 AHVFMGMGVVFEEGVERVGRLPSSIMGCG 267
>gb|AL502543.1|AL502543 AL502543 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW07O19u 3', mRNA sequence
Length = 550
Score = 117 bits (251), Expect = 4e-026
Identities = 42/66 (63%), Positives = 48/66 (72%)
Frame = -3 / -3
Query: 341 NLRDPPLRNTAVIFPYGWTMLRFVADNPGVWAFHCHIEPHLHMGMGVIFAEAVDLVAKVP 162
NLRDP ++NT + PYGWT LRF ADNPGVWAFHCHIE H MGMGV F E ++ V K+P
Sbjct: 488 NLRDPVMKNTVAVHPYGWTALRFRADNPGVWAFHCHIEAHFFMGMGVAFEEGIERVGKLP 309
Query: 161 NEAVSC 144
E C
Sbjct: 308 EEITRC 291
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 254,766,051
Number of Sequences: 312970
Number of extensions: 3892994
Number of successful extensions: 94567
Number of sequences better than 1.0e-020: 17
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 6489756477
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)