TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2430806.2.1
(1380 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AL507045.1|AL507045 AL507045 Hordeum vulgare Barke devel... 173 2e-085
gb|AW982442.3|AW982442 HVSMEg0003F01f Hordeum vulgare pre-a... 285 5e-076
gb|BU974530.1|BU974530 HB28D11r BC Hordeum vulgare subsp. v... 240 5e-069
gb|CB881091.1|CB881091 HM08L24w HM Hordeum vulgare subsp. v... 254 9e-067
gb|AV916696.1|AV916696 AV916696 K. Sato unpublished cDNA li... 219 3e-064
gb|AL502019.1|AL502019 AL502019 Hordeum vulgare Barke roots... 236 3e-061
gb|BE455881.2|BE455881 HVSMEg0017B06f Hordeum vulgare pre-a... 107 2e-056
gb|BU989150.1|BU989150 HF19P04r HF Hordeum vulgare subsp. v... 128 3e-056
gb|BU994960.1|BU994960 HM08L24r HM Hordeum vulgare subsp. v... 210 9e-055
gb|BE060861.3|BE060861 HVSMEg0013K01f Hordeum vulgare pre-a... 170 3e-051
gb|AV921988.1|AV921988 AV921988 K. Sato unpublished cDNA li... 188 9e-047
gb|BE602462.1|BE602462 HVSMEh0098P18f Hordeum vulgare 5-45 ... 105 7e-042
gb|BF628143.2|BF628143 HVSMEb0003E01f Hordeum vulgare seedl... 105 8e-040
gb|CK125868.1|CK125868 BES1824111A1e12 BES1824 Hordeum vulg... 162 5e-039
gb|BJ469249.1|BJ469249 BJ469249 K. Sato unpublished cDNA li... 113 3e-024
gb|BJ474454.1|BJ474454 BJ474454 K. Sato unpublished cDNA li... 113 3e-024
>gb|AL507045.1|AL507045 AL507045 Hordeum vulgare Barke developing caryopsis (3.-15.DAP)
Hordeum vulgare subsp. vulgare cDNA clone HY05A15T 5',
mRNA sequence
Length = 659
Score = 173 bits (372), Expect(5) = 2e-085
Identities = 69/92 (75%), Positives = 81/92 (88%)
Frame = +2 / +1
Query: 209 GRVPLVMAFLSCLAWLYVAGRLWQDAQTRAILSGLLEKSSTSVPKVLSVEDKLRNLGCKA 388
GRVPLVMAFL+CLAW+YVAGRLWQDAQTRAILSGLLEK++ ++PK +SV+DKLR+LGC
Sbjct: 34 GRVPLVMAFLACLAWVYVAGRLWQDAQTRAILSGLLEKNTGNLPKEISVDDKLRDLGCTG 213
Query: 389 IGRKIVEAEMDLTKAKSEGYLWGNRTAAVDSD 484
IGRKI EAEMD+ KAKSEGYLWGN T ++
Sbjct: 214 IGRKIAEAEMDIAKAKSEGYLWGNGTGGTGTE 309
Score = 158 bits (339), Expect(2) = 1e-059
Identities = 66/107 (61%), Positives = 80/107 (74%)
Frame = +2 / +1
Query: 455 GNRTAAVDSDKKQQLLAVIGVYTGFGSRLKRNVFRGSWMPRGDALKKLEEKGVAIRFVIG 634
G + ++++LAVIGVYTGFGSRLKRN FRGSWMPRGDALKKLEEKGV IRFVIG
Sbjct: 286 GTGGTGTEGSDRKKILAVIGVYTGFGSRLKRNTFRGSWMPRGDALKKLEEKGVVIRFVIG 465
Query: 635 RSANRGDSLDRNIDDENRQTKDFLILESHEEAAEELPSKAKIFFSAA 775
RS NRGDS DRNI++E+R+T DFLILE + + K+ +F +
Sbjct: 466 RSPNRGDSXDRNINEESRKTNDFLILERSRGSCRGIT*KS*VFLQCS 606
Score = 149 bits (319), Expect(4) = 1e-073
Identities = 64/86 (74%), Positives = 70/86 (81%)
Frame = -1 / -1
Query: 465 VLFPHRYPSLLALVRSISASTIFLPIALHPRFLSLSSTESTFGTLVELFSRRPDRIARVC 286
V FPH+YPSL AL SISAS IFLPI +HPR LSLSSTE + G L FSRRP+R+ARVC
Sbjct: 290 VPFPHKYPSLFALAMSISASAIFLPIPVHPRSLSLSSTEISLGRLPVFFSRRPERMARVC 111
Query: 285 ASCHKRPAT*SQARQERKAMTRGTLP 208
ASCH RPAT +QARQ RKAMTRGTLP
Sbjct: 110 ASCHSRPATYTQARQARKAMTRGTLP 33
Score = 143 bits (307), Expect(2) = 1e-065
Identities = 60/92 (65%), Positives = 70/92 (76%)
Frame = -3 / -3
Query: 484 VGVHSCSPVPPQVPLALGLGQVHLRLNNFPSNRLAPKVPKLVLHREHLRDAGRALLEEAG 305
+G + SP+PPQVPLAL LG VHLRL + P N A +VP+LV+HR+ L + LLEEAG
Sbjct: 309 LGTSTASPIPPQVPLALRLGNVHLRLGDLPPNPSASEVPQLVVHRDLLGEIAGVLLEEAG 130
Query: 304 QDRPGLRVLPQTAGDVEPGEAGEEGHDERDPA 209
+D PGLRVLP AGDV PGEAGEEGHDE DPA
Sbjct: 129 EDGPGLRVLP*PAGDVHPGEAGEEGHDEGDPA 34
Score = 132 bits (283), Expect(4) = 1e-073
Identities = 59/71 (83%), Positives = 60/71 (84%)
Frame = -1 / -1
Query: 711 RIKKSFVCRFSSSILRSKLSPRFALRPITKRIATPFSSSFFRASPLGIQEPRKTFLLRRE 532
RIKKSFV SS ILRS LSPR LRPITKRI TPFSSSFF+ASPLGIQEPRK FLL RE
Sbjct: 542 RIKKSFVFLLSSLILRSXLSPRLGLRPITKRITTPFSSSFFKASPLGIQEPRKVFLLSRE 363
Query: 531 PNPV*TPMTAR 499
P PV*TPMTAR
Sbjct: 362 PKPV*TPMTAR 330
Score = 127 bits (272), Expect(2) = 1e-065
Identities = 55/75 (73%), Positives = 62/75 (82%)
Frame = -3 / -3
Query: 715 FKNQEVLCLPIFIINITVQAITSICTPTNNKTNSYTLFLKLLQSITSWHPGATEDVPLET 536
F+NQE++CL F INITV AITS+ TP NNKTN++TLFLKLL SITS HPGATE VPLE
Sbjct: 546 FQNQEIVCLSAFFINITVXAITSVRTPANNKTNNHTLFLKLL*SITSGHPGATEGVPLEP 367
Query: 535 GAKPSVNSNDGKKLL 491
GA+ SVNSND K LL
Sbjct: 366 GAEASVNSNDRKDLL 322
Score = 127 bits (271), Expect(5) = 2e-085
Identities = 55/79 (69%), Positives = 60/79 (75%)
Frame = +1 / +3
Query: 502 CRHWSLHWVWLPSQEERLPWLLDAKR*CSEEA*GKGCSYSFCYWSECKSR**LGP*Y*** 681
C HWSLHW+ LP+QEE LPWLLDA+R*C +EA*GKGC YSFCYW E R**LGP*Y**
Sbjct: 333 CGHWSLHWLRLPAQEEHLPWLLDAQR*CFKEA*GKGCGYSFCYWPES*PR**LGP*Y**R 512
Query: 682 KSADKGLLDS*KS*RGCRR 738
K DK LDS K R +R
Sbjct: 513 KQKDKRFLDSGKVTRKLQR 569
Score = 93.2 bits (197), Expect(2) = 1e-059
Identities = 40/64 (62%), Positives = 47/64 (73%)
Frame = +1 / +3
Query: 274 VAGRADPGDPVRPPREELYQRPEGALGGGQA*EPWVQGDWKENC*GGDGPDQGQERGVPV 453
+AGRADPG P+RPPREE Q P+G LGG QA P + DW+E+ GGDG QG+ERGV V
Sbjct: 99 MAGRADPGHPLRPPREEHRQSPQGDLGGRQAEGPRMHWDWEEDRRGGDGHCQGEERGVLV 278
Query: 454 GEQD 465
GE D
Sbjct: 279 GEWD 290
Score = 40.5 bits (82), Expect(5) = 2e-085
Identities = 16/21 (76%), Positives = 17/21 (80%)
Frame = +2 / +2
Query: 713 ESHEEAAEELPSKAKIFFSAA 775
+ HEEAAEELP K K FFSAA
Sbjct: 545 KGHEEAAEELPKKVKFFFSAA 607
Score = 30.9 bits (61), Expect(4) = 1e-073
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = -3 / -2
Query: 775 CSTEENFCFAR*FFCSLFMTF 713
C TEE F R*F CS +TF
Sbjct: 607 CCTEEKLNFFR*FLCSFLVTF 545
Score = 30.4 bits (60), Expect(5) = 2e-085
Identities = 10/11 (90%), Positives = 11/11 (100%)
Frame = +2 / +3
Query: 776 VEAWDAEFYVK 808
+EAWDAEFYVK
Sbjct: 609 IEAWDAEFYVK 641
Score = 22.6 bits (43), Expect(4) = 1e-073
Identities = 7/10 (70%), Positives = 8/10 (80%)
Frame = -3 / -1
Query: 808 FDIKFCIPGF 779
FDI+ CIP F
Sbjct: 641 FDIELCIPSF 612
Score = 22.1 bits (42), Expect(5) = 2e-085
Identities = 10/18 (55%), Positives = 10/18 (55%)
Frame = +1 / +3
Query: 178 LLLCFLRPRLGQGPSRHG 231
LLL R QGP RHG
Sbjct: 3 LLLLLRRRLRRQGPPRHG 56
>gb|AW982442.3|AW982442 HVSMEg0003F01f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0003F01f, mRNA sequence
Length = 589
Score = 285 bits (616), Expect = 5e-076
Identities = 113/125 (90%), Positives = 118/125 (94%)
Frame = +2 / +3
Query: 800 YVKVEDNINLDLAGLIEMLEGRRGSQGLYMGCMKSGMVISEEGQQWYEPEWWKFGDSKTY 979
Y+KV+DNINLDLAGLIEML GRRGS GLYMGCMKSG V+SEE QQWYEPEWWKFGDS+TY
Sbjct: 3 YIKVDDNINLDLAGLIEMLAGRRGSHGLYMGCMKSGAVVSEEEQQWYEPEWWKFGDSRTY 182
Query: 980 FRHASGSLFILSKNLARYININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCCSSSRQ 1159
FRHASGSLFILS NLARYININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCC SS Q
Sbjct: 183 FRHASGSLFILSNNLARYININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCCLSSVQ 362
Query: 1160 EKICS 1174
EK+CS
Sbjct: 363 EKVCS 377
Score = 248 bits (537), Expect = 4e-065
Identities = 104/125 (83%), Positives = 108/125 (86%)
Frame = -1 / -1
Query: 1206 RPAATPSAQALEQIFSCLELLQHNRSSST*VAFSPIIHEPTDISSCA*LCSDALFMLM*R 1027
R AA PSA +E FSC EL QH RSSST*VA SPIIHEPTD+SSCA*LCSDALFMLM*R
Sbjct: 409 RGAAGPSAHPMEHTFSCTELRQHRRSSST*VALSPIIHEPTDMSSCA*LCSDALFMLM*R 230
Query: 1026 AKFFERINKEPEACRKYVFESPNFHHSGSYHCWPSSLMTIPDFIQPMYNPWLPRRPSSIS 847
AK FERINKEPEA RKYV ESPNFHHSGSYHC SSL T PDF+QPMYNPWLPRRP+SIS
Sbjct: 229 AKLFERINKEPEAWRKYVLESPNFHHSGSYHCCSSSLTTAPDFMQPMYNPWLPRRPASIS 50
Query: 846 INPAK 832
INPAK
Sbjct: 49 INPAK 35
Score = 194 bits (419), Expect = 7e-049
Identities = 90/136 (66%), Positives = 104/136 (76%)
Frame = +1 / +2
Query: 799 LCQS*R*H*S*LSWVN*DARRPSR*PRIVHGLYEIRDGHK*RGPTMV*T*VVEIWRFKNV 978
L QS*R*+*S*L WVN*DA PSR*P +VHGL+EIR *RG TMV*T*+VEIWRFK+V
Sbjct: 2 LYQS*R*Y*S*LGWVN*DACWPSR*PWVVHGLHEIRSCR**RGATMV*T*MVEIWRFKDV 181
Query: 979 FSTRFWFFVYPLKKFGSLHQHK*CVTAELCTR*YISRFMDDGTECNLRG**PVVLQQFQT 1158
FS FWFF+YPLK+FGSLHQHK C+ A+LC +I FMDDG C+LR * VL QF T
Sbjct: 182 FSPCFWFFIYPLKQFGSLHQHKQCIAAKLCA*RHIGWFMDDGA*CHLR**RSSVLPQFST 361
Query: 1159 RENLFQCLSGRRGCWP 1206
RE++F +SGR C P
Sbjct: 362 RESMFHRMSGRTSCPP 409
Score = 190 bits (409), Expect = 2e-047
Identities = 90/137 (65%), Positives = 105/137 (76%)
Frame = -3 / -3
Query: 1210 QQASSHAFRSSIGTDFLLSGTAAAQPVIIHVSCIQSHHP*TY*YIIVCITLQ*RTIYVDV 1031
Q+ S +FRSS GT FLL T A Q ++I+VS I+ HHP*T Y+++ I LQ T+YVDV
Sbjct: 413 QKGGSWSFRSSDGTYFLLY*TEATQTIVINVSGIKPHHP*TNRYVVMRIALQRCTVYVDV 234
Query: 1030 ASQIF*EDKQRTRSVSKIRF*ISKFPPLRFIPLLALFTYDHP*FHTTHVQSLATSTAFEH 851
ASQI *EDK RTRS++KIR *ISKFPP RFIPLL LFT D *FH HVQ +ATSTA +H
Sbjct: 233 ASQIV*EDK*RTRSMAKIRP*ISKFPPFRFIPLLLLFTNDSS*FHAAHVQPMATSTASKH 54
Query: 850 LN*PS*VKINVIFNFDI 800
LN*PS VKIN+I NFDI
Sbjct: 53 LN*PSQVKINIIVNFDI 3
Score = 173 bits (372), Expect = 2e-042
Identities = 85/136 (62%), Positives = 91/136 (66%)
Frame = -2 / -2
Query: 1205 GQQPRLPLKHWNRFSLVWNCCSTTGHHPRKLHSVPSSMNLLIYHRVHNSAVTHYLC*CSE 1026
G Q LPL WN SLV N +T H RK H PSSMN I H+ A H LC*CSE
Sbjct: 408 GGQLVLPLIRWNILSLVLN*GNTDDRHQRKWH*APSSMNQPICRHAHSFAAMHCLC*CSE 229
Query: 1025 PNFLRG*TKNQKRVENTFLNLQISTTQVHTIVGPLHL*PSLISYNPCTILGYLDGLRASQ 846
PN LRG* KNQK ENT LNLQIST QVHTIV PLH LIS +PCT GYLDG +ASQ
Sbjct: 228 PNCLRG*IKNQKHGENTSLNLQISTIQVHTIVAPLH*RQLLISCSPCTTHGYLDGQQASQ 49
Query: 845 LTQLSQD*CYLQL*HK 798
LTQ SQD* Y QL*++
Sbjct: 48 LTQPSQD*YYRQL*YR 1
Score = 153 bits (329), Expect = 2e-036
Identities = 73/114 (64%), Positives = 78/114 (68%)
Frame = +3 / +1
Query: 849 RCSKAVEVAKDCTWVV*NQGWS*VKRANNGMNLSGGNLEIQKRIFDTLLVLCLSSQKIWL 1028
RC AVEVA CTW *NQ S VKR+NNGMNL+GGNLEIQ RIF LLVL LSSQ IWL
Sbjct: 52 RCLLAVEVAMGCTWAA*NQELSLVKRSNNGMNLNGGNLEIQGRIFAMLLVLYLSSQTIWL 231
Query: 1029 ATST*IVRHCRVMHTMIYQ*VHG*WD*MQLTWMMTGCAAAVPDKRKSVPMLERK 1190
ATST* V C+ M Y+ VHG*W M LT M C A+V KRK VP ERK
Sbjct: 232 ATST*TVHRCKAMRMTTYRLVHG*WGLMPLTLMTIVCVASVQYKRKYVPSDERK 393
>gb|BU974530.1|BU974530 HB28D11r BC Hordeum vulgare subsp. vulgare cDNA clone HB28D11
5-PRIME, mRNA sequence
Length = 467
Score = 240 bits (519), Expect(2) = 5e-069
Identities = 99/127 (77%), Positives = 110/127 (86%)
Frame = +2 / +3
Query: 455 GNRTAAVDSDKKQQLLAVIGVYTGFGSRLKRNVFRGSWMPRGDALKKLEEKGVAIRFVIG 634
G + ++++LAVIGVYTGFGSRLKRN FRGSWMPRGDALKKLEEKGV I FVIG
Sbjct: 87 GTGGTGTEGSDRKKILAVIGVYTGFGSRLKRNTFRGSWMPRGDALKKLEEKGVVICFVIG 266
Query: 635 RSANRGDSLDRNIDDENRQTKDFLILESHEEAAEELPSKAKIFFSAAVEAWDAEFYVKVE 814
RS NRGDSLDRNI++E+R+T DFLILESHEEAAEELP K K FFSAA+EAWDAEFYVKV+
Sbjct: 267 RSPNRGDSLDRNINEESRKTNDFLILESHEEAAEELPKKVKFFFSAAIEAWDAEFYVKVD 446
Query: 815 DNINLDL 835
DNINLDL
Sbjct: 447 DNINLDL 467
Score = 188 bits (405), Expect(2) = 1e-056
Identities = 80/114 (70%), Positives = 90/114 (78%)
Frame = -3 / -1
Query: 832 VKINVIFNFDIKFCIPGFNCSTEENFCFAR*FFCSLFMTFKNQEVLCLPIFIINITVQAI 653
VKIN+I NFDI+ CIP F C TEE F R*F CS +TF+NQE++CL F INITVQAI
Sbjct: 464 VKINIIVNFDIELCIPSFYCCTEEKLNFFR*FLCSFLVTFQNQEIVCLSAFFINITVQAI 285
Query: 652 TSICTPTNNKTNSYTLFLKLLQSITSWHPGATEDVPLETGAKPSVNSNDGKKLL 491
TS+ TP NNKTN++TLFLKLL SITS HPGATE VPLE GA+ SVNSND K LL
Sbjct: 284 TSVRTPANNKTNNHTLFLKLL*SITSGHPGATEGVPLEPGAEASVNSNDRKDLL 123
Score = 171 bits (368), Expect(2) = 6e-053
Identities = 78/111 (70%), Positives = 86/111 (77%)
Frame = +1 / +2
Query: 502 CRHWSLHWVWLPSQEERLPWLLDAKR*CSEEA*GKGCSYSFCYWSECKSR**LGP*Y*** 681
C HWSLHW+ LP+QEE LPWLLDA+R*C +EA*GKGC Y FCYW E R**LGP*Y**
Sbjct: 134 CGHWSLHWLRLPAQEEHLPWLLDAQR*CFKEA*GKGCGYLFCYWPES*PR**LGP*Y**R 313
Query: 682 KSADKGLLDS*KS*RGCRRITEQSKNFLQCCS*SLGCRILCQS*R*H*S*L 834
K DK LDS KS CR IT +S FLQC + SLGCR+LCQS*R*+*S*L
Sbjct: 314 KQKDKRFLDSGKSRGSCRGIT*KS*VFLQCSNRSLGCRVLCQS*R*Y*S*L 466
Score = 133 bits (285), Expect(2) = 1e-038
Identities = 59/71 (83%), Positives = 61/71 (85%)
Frame = -1 / -2
Query: 711 RIKKSFVCRFSSSILRSKLSPRFALRPITKRIATPFSSSFFRASPLGIQEPRKTFLLRRE 532
RIKKSFV SS ILRSKLSPR LRPITK+I TPFSSSFF+ASPLGIQEPRK FLL RE
Sbjct: 343 RIKKSFVFLLSSLILRSKLSPRLGLRPITKQITTPFSSSFFKASPLGIQEPRKVFLLSRE 164
Query: 531 PNPV*TPMTAR 499
P PV*TPMTAR
Sbjct: 163 PKPV*TPMTAR 131
Score = 124 bits (265), Expect = 1e-027
Identities = 65/115 (56%), Positives = 75/115 (65%)
Frame = -2 / -3
Query: 833 SQD*CYLQL*HKILHPRLQLQH*RKFLLCSVILLQPLHDFQESRSPLSADFHHQYYGPSY 654
SQD* Y QL*H+ LHP+L L H*RK L VI LQ DF ESR+ LS F H YYGPSY
Sbjct: 465 SQD*YYRQL*HRTLHPKLLLLH*RKT*LF*VIPLQLPRDFPESRNRLSFCFLH*YYGPSY 286
Query: 653 HLDLHSDQ*QNE*LHPFPQASSEHHLLAXXXXXXXXXXXXSQTQCKLQ*RQEAAA 489
HL S Q*QN+* HPFPQAS +HHL A S++QCKLQ* Q +++
Sbjct: 285 HLG*DSGQ*QNK*PHPFPQASLKHHLWASRSHGRCSS*AGSRSQCKLQ*PQGSSS 121
Score = 57.4 bits (119), Expect(2) = 6e-053
Identities = 22/32 (68%), Positives = 25/32 (78%)
Frame = +2 / +3
Query: 389 IGRKIVEAEMDLTKAKSEGYLWGNRTAAVDSD 484
IGRKI EAEMD+ KAKSEGYLWGN T ++
Sbjct: 15 IGRKIAEAEMDIAKAKSEGYLWGNGTGGTGTE 110
Score = 52.8 bits (109), Expect(2) = 1e-056
Identities = 20/32 (62%), Positives = 24/32 (75%)
Frame = -3 / -1
Query: 484 VGVHSCSPVPPQVPLALGLGQVHLRLNNFPSN 389
+G + SP+PPQVPLAL LG VHLRL + P N
Sbjct: 110 LGTSTASPIPPQVPLALRLGNVHLRLGDLPPN 15
Score = 47.8 bits (98), Expect(2) = 1e-038
Identities = 20/26 (76%), Positives = 21/26 (80%)
Frame = -1 / -2
Query: 465 VLFPHRYPSLLALVRSISASTIFLPI 388
V FPH+YPSL AL SISAS IFLPI
Sbjct: 91 VPFPHKYPSLFALAMSISASAIFLPI 14
Score = 41.8 bits (85), Expect(2) = 5e-069
Identities = 17/26 (65%), Positives = 20/26 (76%)
Frame = +1 / +2
Query: 388 DWKENC*GGDGPDQGQERGVPVGEQD 465
DW+E+ GGDG QG+ERGV VGE D
Sbjct: 14 DWEEDRRGGDGHCQGEERGVLVGEWD 91
>gb|CB881091.1|CB881091 HM08L24w HM Hordeum vulgare subsp. vulgare cDNA clone HM08L24
3-PRIME, mRNA sequence
Length = 588
Score = 254 bits (549), Expect = 9e-067
Identities = 101/111 (90%), Positives = 104/111 (93%)
Frame = +2 / -2
Query: 842 LIEMLEGRRGSQGLYMGCMKSGMVISEEGQQWYEPEWWKFGDSKTYFRHASGSLFILSKN 1021
LIEML GRRGS GLYMGCMKSG V+SEE QQWYEPEWWKFGDS+TYFRHASGSLFILS N
Sbjct: 587 LIEMLAGRRGSHGLYMGCMKSGAVVSEEEQQWYEPEWWKFGDSRTYFRHASGSLFILSNN 408
Query: 1022 LARYININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCCSSSRQEKICS 1174
LARYININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCC SS QEK+CS
Sbjct: 407 LARYININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCCLSSVQEKVCS 255
Score = 241 bits (521), Expect = 6e-063
Identities = 101/122 (82%), Positives = 105/122 (86%)
Frame = -1 / +1
Query: 1206 RPAATPSAQALEQIFSCLELLQHNRSSST*VAFSPIIHEPTDISSCA*LCSDALFMLM*R 1027
R AA PSA +E FSC EL QH RSSST*VA SPIIHEPTD+SSCA*LCSDALFMLM*R
Sbjct: 223 RGAAGPSAHPMEHTFSCTELRQHRRSSST*VALSPIIHEPTDMSSCA*LCSDALFMLM*R 402
Query: 1026 AKFFERINKEPEACRKYVFESPNFHHSGSYHCWPSSLMTIPDFIQPMYNPWLPRRPSSIS 847
AK FERINKEPEA RKYV ESPNFHHSGSYHC SSL T PDF+QPMYNPWLPRRP+SIS
Sbjct: 403 AKLFERINKEPEAWRKYVLESPNFHHSGSYHCCSSSLTTAPDFMQPMYNPWLPRRPASIS 582
Query: 846 IN 841
IN
Sbjct: 583 IN 588
Score = 176 bits (378), Expect = 3e-043
Identities = 79/122 (64%), Positives = 92/122 (75%)
Frame = +1 / -1
Query: 841 VN*DARRPSR*PRIVHGLYEIRDGHK*RGPTMV*T*VVEIWRFKNVFSTRFWFFVYPLKK 1020
VN*DA PSR*P +VHGL+EIR *RG TMV*T*+VEIWRFK+VFS FWFF+YPLK+
Sbjct: 588 VN*DACWPSR*PWVVHGLHEIRSCR**RGATMV*T*MVEIWRFKDVFSPCFWFFIYPLKQ 409
Query: 1021 FGSLHQHK*CVTAELCTR*YISRFMDDGTECNLRG**PVVLQQFQTRENLFQCLSGRRGC 1200
FGSLHQHK C+ A+LC +I FMDDG C+LR * VL QF TRE++F +SGR C
Sbjct: 408 FGSLHQHKQCIAAKLCA*RHIGWFMDDGA*CHLR**RSSVLPQFSTRESMFHRMSGRTSC 229
Query: 1201 WP 1206
P
Sbjct: 228 PP 223
Score = 165 bits (355), Expect = 5e-040
Identities = 79/123 (64%), Positives = 93/123 (75%)
Frame = -3 / +3
Query: 1210 QQASSHAFRSSIGTDFLLSGTAAAQPVIIHVSCIQSHHP*TY*YIIVCITLQ*RTIYVDV 1031
Q+ S +FRSS GT FLL T A Q ++I+VS I+ HHP*T Y+++ I LQ T+YVDV
Sbjct: 219 QKGGSWSFRSSDGTYFLLY*TEATQTIVINVSGIKPHHP*TNRYVVMRIALQRCTVYVDV 398
Query: 1030 ASQIF*EDKQRTRSVSKIRF*ISKFPPLRFIPLLALFTYDHP*FHTTHVQSLATSTAFEH 851
ASQI *EDK RTRS++KIR *ISKFPP RFIPLL LFT D *FH HVQ +ATSTA +H
Sbjct: 399 ASQIV*EDK*RTRSMAKIRP*ISKFPPFRFIPLLLLFTNDSS*FHAAHVQPMATSTASKH 578
Query: 850 LN* 842
LN*
Sbjct: 579 LN* 587
Score = 155 bits (332), Expect = 7e-037
Identities = 75/121 (61%), Positives = 79/121 (65%)
Frame = -2 / +2
Query: 1205 GQQPRLPLKHWNRFSLVWNCCSTTGHHPRKLHSVPSSMNLLIYHRVHNSAVTHYLC*CSE 1026
G Q LPL WN SLV N +T H RK H PSSMN I H+ A H LC*CSE
Sbjct: 224 GGQLVLPLIRWNILSLVLN*GNTDDRHQRKWH*APSSMNQPICRHAHSFAAMHCLC*CSE 403
Query: 1025 PNFLRG*TKNQKRVENTFLNLQISTTQVHTIVGPLHL*PSLISYNPCTILGYLDGLRASQ 846
PN LRG* KNQK ENT LNLQIST QVHTIV PLH LIS +PCT GYLDG +ASQ
Sbjct: 404 PNCLRG*IKNQKHGENTSLNLQISTIQVHTIVAPLH*RQLLISCSPCTTHGYLDGQQASQ 583
Query: 845 L 843
L
Sbjct: 584 L 586
Score = 153 bits (329), Expect = 2e-036
Identities = 73/114 (64%), Positives = 78/114 (68%)
Frame = +3 / -3
Query: 849 RCSKAVEVAKDCTWVV*NQGWS*VKRANNGMNLSGGNLEIQKRIFDTLLVLCLSSQKIWL 1028
RC AVEVA CTW *NQ S VKR+NNGMNL+GGNLEIQ RIF LLVL LSSQ IWL
Sbjct: 580 RCLLAVEVAMGCTWAA*NQELSLVKRSNNGMNLNGGNLEIQGRIFAMLLVLYLSSQTIWL 401
Query: 1029 ATST*IVRHCRVMHTMIYQ*VHG*WD*MQLTWMMTGCAAAVPDKRKSVPMLERK 1190
ATST* V C+ M Y+ VHG*W M LT M C A+V KRK VP ERK
Sbjct: 400 ATST*TVHRCKAMRMTTYRLVHG*WGLMPLTLMTIVCVASVQYKRKYVPSDERK 239
>gb|AV916696.1|AV916696 AV916696 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19f02 5', mRNA sequence
Length = 652
Score = 219 bits (473), Expect(2) = 3e-064
Identities = 84/147 (57%), Positives = 111/147 (75%)
Frame = +2 / +3
Query: 599 EEKGVAIRFVIGRSANRGDSLDRNIDDENRQTKDFLILESHEEAAEELPSKAKIFFSAAV 778
+EKG+A+RF++GRSANRGD DR IDDENR T DF+IL+ H E+ EELP K K +F+ A
Sbjct: 210 DEKGIAVRFIVGRSANRGDRFDREIDDENRSTNDFVILDDHTESDEELPKKTKRYFANAA 389
Query: 779 EAWDAEFYVKVEDNINLDLAGLIEMLEGRRGSQGLYMGCMKSGMVISEEGQQWYEPEWWK 958
E +DAEFY KV D+I +++ L MLE +Y+GCMKSG V SE +WYEP+WWK
Sbjct: 390 ETFDAEFYAKVNDDIYINVDTLSAMLETHWDKPRVYIGCMKSGEVFSEATHKWYEPDWWK 569
Query: 959 FGDSKTYFRHASGSLFILSKNLARYIN 1039
FGD K+YFRHASG +FI+S+ +A++I+
Sbjct: 570 FGDGKSYFRHASGEMFIVSRAVAQFIS 650
Score = 107 bits (229), Expect = 1e-022
Identities = 48/132 (36%), Positives = 75/132 (56%)
Frame = -3 / -3
Query: 1009 DKQRTRSVSKIRF*ISKFPPLRFIPLLALFTYDHP*FHTTHVQSLATSTAFEHLN*PS*V 830
+K TRS+SK+ F ++KFPP+ FIPL+ F D P FHT +V + +F+H +
Sbjct: 620 NKHFTRSMSKV*FPVTKFPPIWFIPLMGRFRKDLPRFHTAYVDTRFVPVSFKHCTQSVNI 441
Query: 829 KINVIFNFDIKFCIPGFNCSTEENFCFAR*FFCSLFMTFKNQEVLCLPIFIINITVQAIT 650
+ +I + IKF I ++ FC R F L M +N +++C I +I+ V+ I+
Sbjct: 440 YVYIIIDLSIKFSIKCLCSISKVTFCLLRKLFIRLCMIIQNHKIICTSILVIDFPVKTIS 261
Query: 649 SICTPTNNKTNS 614
SICT +N +T S
Sbjct: 260 SICTSSNYETYS 225
Score = 80.8 bits (170), Expect(2) = 2e-025
Identities = 35/61 (57%), Positives = 40/61 (65%)
Frame = -1 / -1
Query: 1026 AKFFERINKEPEACRKYVFESPNFHHSGSYHCWPSSLMTIPDFIQPMYNPWLPRRPSSIS 847
A + IN PEACRKY F SPNFH SGSYH W +S T PDFIQPM L + SSI+
Sbjct: 637 ATALDTINISPEACRKYDFPSPNFHQSGSYHLWVASEKTSPDFIQPM*TRGLSQ*VSSIA 458
Query: 846 I 844
+
Sbjct: 457 L 455
Score = 58.8 bits (122), Expect(2) = 2e-025
Identities = 29/44 (65%), Positives = 31/44 (70%)
Frame = -1 / -1
Query: 711 RIKKSFVCRFSSSILRSKLSPRFALRPITKRIATPFSSSFFRAS 580
+I KSFV FSSSI SK SPRFAL P KR A PFSSS F +S
Sbjct: 322 KITKSFVLLFSSSISLSKRSPRFALLPTMKRTAMPFSSSSFFSS 191
Score = 46.9 bits (96), Expect(2) = 3e-064
Identities = 18/40 (45%), Positives = 27/40 (67%)
Frame = +2 / +3
Query: 488 KQQLLAVIGVYTGFGSRLKRNVFRGSWMPRGDALKKLEEK 607
+++ L VIG+ T FG + R+ R SW+P G LKKLE++
Sbjct: 96 RKKPLVVIGIMTSFGRKNYRDAVRKSWLPTGSLLKKLEDE 215
>gb|AL502019.1|AL502019 AL502019 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW06D11u 3', mRNA sequence
Length = 526
Score = 236 bits (509), Expect = 3e-061
Identities = 92/102 (90%), Positives = 96/102 (94%)
Frame = +2 / -1
Query: 869 GSQGLYMGCMKSGMVISEEGQQWYEPEWWKFGDSKTYFRHASGSLFILSKNLARYININS 1048
G+ GLYMGCMKSG V+SEE QQWYEPEWWKFGDS+TYFRHASGSLFILS NLARYININS
Sbjct: 523 GNHGLYMGCMKSGAVVSEEEQQWYEPEWWKFGDSRTYFRHASGSLFILSNNLARYININS 344
Query: 1049 ASLQSYAHDDISVGSWMMGLNATYVDDDRLCCSSSRQEKICS 1174
ASLQSYAHDDISVGSWMMGLNATYVDDDRLCC SS QEK+CS
Sbjct: 343 ASLQSYAHDDISVGSWMMGLNATYVDDDRLCCLSSVQEKVCS 218
Score = 223 bits (481), Expect(2) = 2e-057
Identities = 93/113 (82%), Positives = 96/113 (84%)
Frame = -1 / +3
Query: 1206 RPAATPSAQALEQIFSCLELLQHNRSSST*VAFSPIIHEPTDISSCA*LCSDALFMLM*R 1027
R AA PSA +E FSC EL QH RSSST*VA SPIIHEPTD+SSCA*LCSDALFMLM*R
Sbjct: 186 RGAAGPSAHPMEHTFSCTELRQHRRSSST*VALSPIIHEPTDMSSCA*LCSDALFMLM*R 365
Query: 1026 AKFFERINKEPEACRKYVFESPNFHHSGSYHCWPSSLMTIPDFIQPMYNPWLP 868
AK FERINKEPEA RKYV ESPNFHHSGSYHC SSL T PDF+QPMYNPWLP
Sbjct: 366 AKLFERINKEPEAWRKYVLESPNFHHSGSYHCCSSSLTTAPDFMQPMYNPWLP 524
Score = 161 bits (347), Expect = 6e-039
Identities = 71/112 (63%), Positives = 84/112 (75%)
Frame = +1 / -3
Query: 871 *PRIVHGLYEIRDGHK*RGPTMV*T*VVEIWRFKNVFSTRFWFFVYPLKKFGSLHQHK*C 1050
*P +VHGL+EIR *RG TMV*T*+VEIWRFK+VFS FWFF+YPLK+FGSLHQHK C
Sbjct: 521 *PWVVHGLHEIRSCR**RGATMV*T*MVEIWRFKDVFSPCFWFFIYPLKQFGSLHQHKQC 342
Query: 1051 VTAELCTR*YISRFMDDGTECNLRG**PVVLQQFQTRENLFQCLSGRRGCWP 1206
+ A+LC +I FMDDG C+LR * VL QF TRE++F +SGR C P
Sbjct: 341 IAAKLCA*RHIGWFMDDGA*CHLR**RSSVLPQFSTRESMFHRMSGRTSCPP 186
Score = 149 bits (320), Expect = 3e-035
Identities = 71/114 (62%), Positives = 84/114 (73%)
Frame = -3 / +2
Query: 1210 QQASSHAFRSSIGTDFLLSGTAAAQPVIIHVSCIQSHHP*TY*YIIVCITLQ*RTIYVDV 1031
Q+ S +FRSS GT FLL T A Q ++I+VS I+ HHP*T Y+++ I LQ T+YVDV
Sbjct: 182 QKGGSWSFRSSDGTYFLLY*TEATQTIVINVSGIKPHHP*TNRYVVMRIALQRCTVYVDV 361
Query: 1030 ASQIF*EDKQRTRSVSKIRF*ISKFPPLRFIPLLALFTYDHP*FHTTHVQSLAT 869
ASQI *EDK RTRS++KIR *ISKFPP RFIPLL LFT D *FH HVQ + T
Sbjct: 362 ASQIV*EDK*RTRSMAKIRP*ISKFPPFRFIPLLLLFTNDSS*FHAAHVQPMVT 523
Score = 141 bits (302), Expect = 1e-032
Identities = 67/107 (62%), Positives = 72/107 (67%)
Frame = +3 / -2
Query: 870 VAKDCTWVV*NQGWS*VKRANNGMNLSGGNLEIQKRIFDTLLVLCLSSQKIWLATST*IV 1049
V CTW *NQ S VKR+NNGMNL+GGNLEIQ RIF LLVL LSSQ IWLATST* V
Sbjct: 522 VTMGCTWAA*NQELSLVKRSNNGMNLNGGNLEIQGRIFAMLLVLYLSSQTIWLATST*TV 343
Query: 1050 RHCRVMHTMIYQ*VHG*WD*MQLTWMMTGCAAAVPDKRKSVPMLERK 1190
C+ M Y+ VHG*W M LT M C A+V KRK VP ERK
Sbjct: 342 HRCKAMRMTTYRLVHG*WGLMPLTLMTIVCVASVQYKRKYVPSDERK 202
Score = 140 bits (300), Expect = 2e-032
Identities = 68/112 (60%), Positives = 71/112 (63%)
Frame = -2 / +1
Query: 1205 GQQPRLPLKHWNRFSLVWNCCSTTGHHPRKLHSVPSSMNLLIYHRVHNSAVTHYLC*CSE 1026
G Q LPL WN SLV N +T H RK H PSSMN I H+ A H LC*CSE
Sbjct: 187 GGQLVLPLIRWNILSLVLN*GNTDDRHQRKWH*APSSMNQPICRHAHSFAAMHCLC*CSE 366
Query: 1025 PNFLRG*TKNQKRVENTFLNLQISTTQVHTIVGPLHL*PSLISYNPCTILGY 870
PN LRG* KNQK ENT LNLQIST QVHTIV PLH LIS +PCT GY
Sbjct: 367 PNCLRG*IKNQKHGENTSLNLQISTIQVHTIVAPLH*RQLLISCSPCTTHGY 522
Score = 23.5 bits (45), Expect(2) = 2e-057
Identities = 8/11 (72%), Positives = 8/11 (72%)
Frame = -1 / +2
Query: 1380 FFFFFFLMEKI 1348
FFFFFF E I
Sbjct: 14 FFFFFFFFESI 46
>gb|BE455881.2|BE455881 HVSMEg0017B06f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0017B06f, mRNA sequence
Length = 818
Score = 107 bits (229), Expect(4) = 2e-056
Identities = 42/83 (50%), Positives = 60/83 (72%)
Frame = +2 / +1
Query: 719 HEEAAEELPSKAKIFFSAAVEAWDAEFYVKVEDNINLDLAGLIEMLEGRRGSQGLYMGCM 898
H E EL SK +I+F+AAV WDA FYVKV+D+++++L L L R + +Y+GCM
Sbjct: 205 HVEGYHELSSKTRIYFAAAVATWDAAFYVKVDDDVHVNLGMLTNRLARYRTTPRVYVGCM 384
Query: 899 KSGMVISEEGQQWYEPEWWKFGD 967
KSG V+S++G +++EPE WKFGD
Sbjct: 385 KSGPVLSQKGVKYHEPESWKFGD 453
Score = 75.8 bits (159), Expect(4) = 2e-056
Identities = 26/67 (38%), Positives = 44/67 (65%)
Frame = +2 / +1
Query: 950 WWKFGDSKTYFRHASGSLFILSKNLARYININSASLQSYAHDDISVGSWMMGLNATYVDD 1129
W + YFRHA+ ++ + ++LA YI IN L +A++D+S+G+W++GL +VDD
Sbjct: 439 WKFGDEGNKYFRHATXQIYAIXRDLAFYIXINQPILHRFANEDVSLGAWLIGLEVEHVDD 618
Query: 1130 DRLCCSS 1150
LCC++
Sbjct: 619 RSLCCAT 639
Score = 54.2 bits (112), Expect(4) = 2e-056
Identities = 23/42 (54%), Positives = 30/42 (71%)
Frame = +2 / +1
Query: 599 EEKGVAIRFVIGRSANRGDSLDRNIDDENRQTKDFLILESHE 724
+EKGV +RFVIG SA G +LDR ID E +T+DF+ L+ E
Sbjct: 88 KEKGVVVRFVIGHSATPGGALDRAIDVEEAETRDFMRLDHVE 213
Score = 39.6 bits (80), Expect(4) = 2e-056
Identities = 13/31 (41%), Positives = 23/31 (74%)
Frame = +2 / +1
Query: 515 VYTGFGSRLKRNVFRGSWMPRGDALKKLEEK 607
+ T F S+ +R+ R +W+PRGD L++LE++
Sbjct: 1 INTAFSSKKRRDSLRDTWVPRGDKLRQLEKE 93
>gb|BU989150.1|BU989150 HF19P04r HF Hordeum vulgare subsp. vulgare cDNA clone HF19P04
5-PRIME, mRNA sequence
Length = 657
Score = 128 bits (275), Expect(2) = 3e-056
Identities = 51/87 (58%), Positives = 66/87 (75%)
Frame = +2 / +1
Query: 599 EEKGVAIRFVIGRSANRGDSLDRNIDDENRQTKDFLILESHEEAAEELPSKAKIFFSAAV 778
+EKG+A+RF++GRSANRGD DR IDDENR T DF+IL+ H E+ EELP K K +F+ A
Sbjct: 394 DEKGIAVRFIVGRSANRGDRFDREIDDENRSTNDFVILDDHTESDEELPKKTKRYFANAA 573
Query: 779 EAWDAEFYVKVEDNINLDLAGLIEMLE 859
E +DAEFY KV D+I +++ L MLE
Sbjct: 574 ETFDAEFYAKVNDDIYINVDTLSAMLE 654
Score = 111 bits (236), Expect(2) = 3e-056
Identities = 47/129 (36%), Positives = 77/129 (59%)
Frame = +2 / +1
Query: 221 LVMAFLSCLAWLYVAGRLWQDAQTRAILSGLLEKSSTSVPKVLSVEDKLRNLGCKAIGRK 400
+++A + +A YVAGRLWQDAQ R L L++ + +SV+D L+ + C+ G++
Sbjct: 13 MMLAMFATMASFYVAGRLWQDAQNRVYLIKELDRRTGQGRSAISVDDTLKVVSCRQQGKR 192
Query: 401 IVEAEMDLTKAKSEGYLWGNRTAAVDSDKKQQLLAVIGVYTGFGSRLKRNVFRGSWMPRG 580
+ EM+L AK EG++ + + +++ L VIG+ T FG + R+ R SW+P G
Sbjct: 193 LASLEMELAAAKHEGFVGKYTSETNATHSRKKPLVVIGIMTSFGRKNYRDAVRKSWLPTG 372
Query: 581 DALKKLEEK 607
LKKLE++
Sbjct: 373 SLLKKLEDE 399
>gb|BU994960.1|BU994960 HM08L24r HM Hordeum vulgare subsp. vulgare cDNA clone HM08L24
5-PRIME, mRNA sequence
Length = 582
Score = 210 bits (453), Expect(2) = 9e-055
Identities = 83/95 (87%), Positives = 87/95 (91%)
Frame = +2 / +1
Query: 890 GCMKSGMVISEEGQQWYEPEWWKFGDSKTYFRHASGSLFILSKNLARYININSASLQSYA 1069
G + G V+SEE QQWYEPEWWKFGDS+TYFRHASGSLFILS NLARYININSASLQSYA
Sbjct: 43 GLHEIGAVVSEEEQQWYEPEWWKFGDSRTYFRHASGSLFILSNNLARYININSASLQSYA 222
Query: 1070 HDDISVGSWMMGLNATYVDDDRLCCSSSRQEKICS 1174
HDDISVGSWMMGLNATYVDDDRLCC SS QEK+CS
Sbjct: 223 HDDISVGSWMMGLNATYVDDDRLCCLSSVQEKVCS 327
Score = 190 bits (410), Expect(2) = 1e-048
Identities = 83/106 (78%), Positives = 85/106 (80%)
Frame = -1 / -2
Query: 1206 RPAATPSAQALEQIFSCLELLQHNRSSST*VAFSPIIHEPTDISSCA*LCSDALFMLM*R 1027
R AA PSA +E FSC EL QH RSSST*VA SPIIHEPTD+SSCA*LCSDALFMLM*R
Sbjct: 359 RGAAGPSAHPMEHTFSCTELRQHRRSSST*VALSPIIHEPTDMSSCA*LCSDALFMLM*R 180
Query: 1026 AKFFERINKEPEACRKYVFESPNFHHSGSYHCWPSSLMTIPDFIQP 889
AK FERINKEPEA RKYV ESPNFHHSGSYHC SSL T P P
Sbjct: 179 AKLFERINKEPEAWRKYVLESPNFHHSGSYHCCSSSLTTAPISCSP 42
Score = 144 bits (308), Expect = 1e-033
Identities = 62/96 (64%), Positives = 73/96 (76%)
Frame = +1 / +3
Query: 919 *RGPTMV*T*VVEIWRFKNVFSTRFWFFVYPLKKFGSLHQHK*CVTAELCTR*YISRFMD 1098
*RG TMV*T*+VEIWRFK+VFS FWFF+YPLK+FGSLHQHK C+ A+LC +I FMD
Sbjct: 72 *RGATMV*T*MVEIWRFKDVFSPCFWFFIYPLKQFGSLHQHKQCIAAKLCA*RHIGWFMD 251
Query: 1099 DGTECNLRG**PVVLQQFQTRENLFQCLSGRRGCWP 1206
DG C+LR * VL QF TRE++F +SGR C P
Sbjct: 252 DGA*CHLR**RSSVLPQFSTRESMFHRMSGRTSCPP 359
Score = 133 bits (284), Expect = 3e-030
Identities = 64/100 (64%), Positives = 76/100 (76%)
Frame = -3 / -1
Query: 1210 QQASSHAFRSSIGTDFLLSGTAAAQPVIIHVSCIQSHHP*TY*YIIVCITLQ*RTIYVDV 1031
Q+ S +FRSS GT FLL T A Q ++I+VS I+ HHP*T Y+++ I LQ T+YVDV
Sbjct: 363 QKGGSWSFRSSDGTYFLLY*TEATQTIVINVSGIKPHHP*TNRYVVMRIALQRCTVYVDV 184
Query: 1030 ASQIF*EDKQRTRSVSKIRF*ISKFPPLRFIPLLALFTYD 911
ASQI *EDK RTRS++KIR *ISKFPP RFIPLL LFT D
Sbjct: 183 ASQIV*EDK*RTRSMAKIRP*ISKFPPFRFIPLLLLFTND 64
Score = 126 bits (270), Expect = 3e-028
Identities = 60/96 (62%), Positives = 62/96 (64%)
Frame = -2 / -3
Query: 1205 GQQPRLPLKHWNRFSLVWNCCSTTGHHPRKLHSVPSSMNLLIYHRVHNSAVTHYLC*CSE 1026
G Q LPL WN SLV N +T H RK H PSSMN I H+ A H LC*CSE
Sbjct: 358 GGQLVLPLIRWNILSLVLN*GNTDDRHQRKWH*APSSMNQPICRHAHSFAAMHCLC*CSE 179
Query: 1025 PNFLRG*TKNQKRVENTFLNLQISTTQVHTIVGPLH 918
PN LRG* KNQK ENT LNLQIST QVHTIV PLH
Sbjct: 178 PNCLRG*IKNQKHGENTSLNLQISTIQVHTIVAPLH 71
Score = 125 bits (267), Expect = 7e-028
Identities = 59/91 (64%), Positives = 64/91 (70%)
Frame = +3 / +2
Query: 918 VKRANNGMNLSGGNLEIQKRIFDTLLVLCLSSQKIWLATST*IVRHCRVMHTMIYQ*VHG 1097
VKR+NNGMNL+GGNLEIQ RIF LLVL LSSQ IWLATST* V C+ M Y+ VHG
Sbjct: 71 VKRSNNGMNLNGGNLEIQGRIFAMLLVLYLSSQTIWLATST*TVHRCKAMRMTTYRLVHG 250
Query: 1098 *WD*MQLTWMMTGCAAAVPDKRKSVPMLERK 1190
*W M LT M C A+V KRK VP ERK
Sbjct: 251 *WGLMPLTLMTIVCVASVQYKRKYVPSDERK 343
Score = 24.4 bits (47), Expect(2) = 9e-055
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = +2 / +1
Query: 845 IEMLEGRRGSQG 880
IEML GRRGS G
Sbjct: 1 IEMLAGRRGSLG 36
Score = 23.5 bits (45), Expect(2) = 1e-048
Identities = 9/11 (81%), Positives = 10/11 (90%)
Frame = -1 / -2
Query: 879 PWLPRRPSSIS 847
P LPRRP+SIS
Sbjct: 35 PRLPRRPASIS 3
>gb|BE060861.3|BE060861 HVSMEg0013K01f Hordeum vulgare pre-anthesis spike EST library
HVcDNA0008 (white to yellow anther) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEg0013K01f, mRNA sequence
Length = 628
Score = 170 bits (365), Expect(3) = 3e-051
Identities = 73/126 (57%), Positives = 87/126 (69%)
Frame = +2 / +2
Query: 98 PASEISAAMETLASAMRRENRRXXXXXXXXXXXXXXXGRVPLVMAFLSCLAWLYVAGRLW 277
P+ EI+A ++ R R GRVPLVMAFL+CLAW+YVAGRLW
Sbjct: 8 PSEEIAAMETLASAMRRDNRRFKPPSSSSSSSAAASAGRVPLVMAFLACLAWVYVAGRLW 187
Query: 278 QDAQTRAILSGLLEKSSTSVPKVLSVEDKLRNLGCKAIGRKIVEAEMDLTKAKSEGYLWG 457
QDAQTRAILSGLLEK++ ++PK +SV+DKLR+LGC IGRKI EAEMD K KSEGYL G
Sbjct: 188 QDAQTRAILSGLLEKNTGNLPKEISVDDKLRDLGCTGIGRKIAEAEMDXAKGKSEGYL*G 367
Query: 458 NRTAAV 475
N T +
Sbjct: 368 NGTGGL 385
Score = 145 bits (312), Expect(4) = 4e-049
Identities = 63/86 (73%), Positives = 70/86 (81%)
Frame = -1 / -2
Query: 465 VLFPHRYPSLLALVRSISASTIFLPIALHPRFLSLSSTESTFGTLVELFSRRPDRIARVC 286
V FP++YPSLL L SISAS IFLPI +HPR LSLSSTE + G L FSRRP+R+ARVC
Sbjct: 375 VPFPYKYPSLLPLAMSISASAIFLPIPVHPRSLSLSSTEISLGRLPVFFSRRPERMARVC 196
Query: 285 ASCHKRPAT*SQARQERKAMTRGTLP 208
ASCH RPAT +QARQ RKAMTRGTLP
Sbjct: 195 ASCHSRPATYTQARQARKAMTRGTLP 118
Score = 134 bits (287), Expect(3) = 1e-038
Identities = 57/88 (64%), Positives = 65/88 (73%)
Frame = -3 / -1
Query: 472 SCSPVPPQVPLALGLGQVHLRLNNFPSNRLAPKVPKLVLHREHLRDAGRALLEEAGQDRP 293
S SP+P QVPLA G VHLRL + P N A +VP+LV+HR+ L + LLEEAG+D P
Sbjct: 382 SASPIPLQVPLAFAFGXVHLRLGDLPPNPSASEVPQLVVHRDLLGEIAGVLLEEAGEDGP 203
Query: 292 GLRVLPQTAGDVEPGEAGEEGHDERDPA 209
GLRVLP AGDV PGEAGEEGHDE DPA
Sbjct: 202 GLRVLP*PAGDVHPGEAGEEGHDEGDPA 119
Score = 90.9 bits (192), Expect(4) = 1e-039
Identities = 40/68 (58%), Positives = 47/68 (69%)
Frame = +1 / +1
Query: 274 VAGRADPGDPVRPPREELYQRPEGALGGGQA*EPWVQGDWKENC*GGDGPDQGQERGVPV 453
+AGRADPG P+RPPREE Q P+G LGG QA P + DW+E+ GGDG Q Q+RGV V
Sbjct: 184 MAGRADPGHPLRPPREEHRQSPQGDLGGRQAEGPRMHWDWEEDRRGGDGHXQRQKRGVLV 363
Query: 454 GEQDCSCG 477
GE D G
Sbjct: 364 GEWDWRTG 387
Score = 63.4 bits (132), Expect(4) = 1e-039
Identities = 26/36 (72%), Positives = 29/36 (80%)
Frame = +1 / +1
Query: 100 SVGDLRRHGDAGQRHAAREPPVQGPALLLCFLRPRL 207
+VG RRHGDAGQRHAAR+PP+Q P LLL LR RL
Sbjct: 7 AVGGDRRHGDAGQRHAARQPPLQAPILLLLLLRRRL 114
Score = 42.8 bits (87), Expect(4) = 4e-049
Identities = 17/20 (85%), Positives = 18/20 (90%)
Frame = -3 / -1
Query: 160 AVLAPHGAGQRLHGGGDLRR 101
AV+APHGAGQRLHGG LRR
Sbjct: 67 AVVAPHGAGQRLHGGDLLRR 8
Score = 39.1 bits (79), Expect(4) = 1e-039
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +1 / +3
Query: 502 CRHWSLHWVWLPSQEERLPWLLDAK 576
C HW L W+ LP+QEE L LL+A+
Sbjct: 417 CGHWRLPWLRLPAQEEHLSGLLEAQ 491
Score = 39.1 bits (79), Expect(3) = 3e-051
Identities = 16/30 (53%), Positives = 21/30 (70%)
Frame = +2 / +1
Query: 488 KQQLLAVIGVYTGFGSRLKRNVFRGSWMPR 577
++++ A IG Y GFG RLKRN F GS P+
Sbjct: 403 RKKIPAAIGGYPGFGFRLKRNTFPGS*KPK 492
Score = 36.8 bits (74), Expect(3) = 1e-038
Identities = 18/22 (81%), Positives = 18/22 (81%)
Frame = -1 / -2
Query: 168 LDRRFSRRMALASVSMAAEISD 103
L RR SRRMALASVSMAA SD
Sbjct: 75 LKRRLSRRMALASVSMAAISSD 10
Score = 33.6 bits (67), Expect(4) = 4e-049
Identities = 15/25 (60%), Positives = 16/25 (64%)
Frame = -1 / -3
Query: 576 LGIQEPRKTFLLRREPNPV*TPMTA 502
LG EP K FLL R+P P * PM A
Sbjct: 491 LGF*EPGKVFLLSRKPKPG*PPMAA 417
Score = 32.2 bits (64), Expect(3) = 3e-051
Identities = 15/46 (32%), Positives = 25/46 (54%)
Frame = +2 / +3
Query: 575 RGDALKKLEEKGVAIRFVIGRSANRGDSLDRNIDDENRQTKDFLIL 712
+ DALK L++KG V+ R+ N G++ + + + DFL L
Sbjct: 489 QSDALKSLQKKGGVFHLVMDRNPNPGEAWTGLLVRKPERQTDFLFL 626
Score = 29.9 bits (59), Expect(4) = 4e-049
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = -1 / -2
Query: 705 KKSFVCRFSSSILRSKLSPRFALRPITKRIATPFSSSFFR 586
K FV R S ++RSKL P+ I PFS+ F +
Sbjct: 618 KNRFVFRAFSLVIRSKLPLG*DFGPLPNEIPHPFSAGFLK 499
Score = 27.6 bits (54), Expect(3) = 1e-038
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = -3 / -2
Query: 565 GATEDVPLETGAKPSVNSNDGKKLLLLVGVHSCSPVP 455
GA + VPLE A+ V SN + L + + + PVP
Sbjct: 480 GARKGVPLEPEAEARVTSNGRRDFLPVRHLGTSPPVP 370
Score = 26.7 bits (52), Expect(4) = 1e-039
Identities = 15/40 (37%), Positives = 20/40 (50%)
Frame = +2 / +2
Query: 590 KKLEEKGVAIRFVIGRSANRGDSLDRNIDDENRQTKDFLI 709
KK EKG I F G + SLDR ++ R+T F +
Sbjct: 503 KKPAEKGWGISFGNGPKS*PRGSLDRITSEKARKTNRFFV 622
>gb|AV921988.1|AV921988 AV921988 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags19f02 3', mRNA sequence
Length = 680
Score = 188 bits (404), Expect = 9e-047
Identities = 72/134 (53%), Positives = 99/134 (73%)
Frame = +2 / -1
Query: 773 AVEAWDAEFYVKVEDNINLDLAGLIEMLEGRRGSQGLYMGCMKSGMVISEEGQQWYEPEW 952
A E +DAEFY KV D+I +++ L MLE +Y+GCMKSG V SE +WYEP+W
Sbjct: 674 AAETFDAEFYAKVNDDIYINVDTLSAMLETHWDKPRVYIGCMKSGEVFSEATHKWYEPDW 495
Query: 953 WKFGDSKTYFRHASGSLFILSKNLARYININSASLQSYAHDDISVGSWMMGLNATYVDDD 1132
WKFGD K+YFRHASG +FI+S+ +A++I+IN + L++YAHDD+SVGSWM+GL +V++
Sbjct: 494 WKFGDGKSYFRHASGEMFIVSRAVAQFISINRSVLRTYAHDDVSVGSWMIGLAVKHVNEA 315
Query: 1133 RLCCSSSRQEKICS 1174
+LCCSS +CS
Sbjct: 314 KLCCSSWPSGAMCS 273
Score = 111 bits (237), Expect = 9e-024
Identities = 54/100 (54%), Positives = 63/100 (63%)
Frame = -1 / +1
Query: 1143 QHNRSSST*VAFSPIIHEPTDISSCA*LCSDALFMLM*RAKFFERINKEPEACRKYVFES 964
QH+ +S T SPIIHEPT SSCA + LF+ M A + IN PEACRKY F S
Sbjct: 304 QHSFASFTCFTASPIIHEPTLTSSCAYVRRTDLFIEMN*ATALDTINISPEACRKYDFPS 483
Query: 963 PNFHHSGSYHCWPSSLMTIPDFIQPMYNPWLPRRPSSISI 844
PNFH SGSYH W +S T PDFIQPM L + SSI++
Sbjct: 484 PNFHQSGSYHLWVASEKTSPDFIQPM*TRGLSQ*VSSIAL 603
>gb|BE602462.1|BE602462 HVSMEh0098P18f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0098P18f, mRNA sequence
Length = 576
Score = 105 bits (223), Expect(2) = 7e-042
Identities = 40/83 (48%), Positives = 59/83 (71%)
Frame = +2 / +1
Query: 719 HEEAAEELPSKAKIFFSAAVEAWDAEFYVKVEDNINLDLAGLIEMLEGRRGSQGLYMGCM 898
H E L K K +F+ AV WDA+FYVKV+D++++++A L ++L + +YMGCM
Sbjct: 67 HVEGYLALSGKTKTYFATAVSLWDADFYVKVDDDVHVNIATLGQILSKQAWKPRVYMGCM 246
Query: 899 KSGMVISEEGQQWYEPEWWKFGD 967
KSG V+SE+G ++YEPE WKFG+
Sbjct: 247 KSGPVLSEKGVRYYEPEHWKFGE 315
Score = 86.8 bits (183), Expect(2) = 7e-042
Identities = 30/58 (51%), Positives = 44/58 (75%)
Frame = +2 / +1
Query: 977 YFRHASGSLFILSKNLARYININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCCSS 1150
YFRHA+G L+ +SK+LA YI+IN L Y ++D+S+GSW +GL+ ++DD RLCC +
Sbjct: 328 YFRHATGQLYAISKDLATYISINKHVLHKYINEDVSLGSWFLGLDVEHIDDRRLCCGT 501
>gb|BF628143.2|BF628143 HVSMEb0003E01f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0003E01f, mRNA sequence
Length = 499
Score = 105 bits (223), Expect(3) = 8e-040
Identities = 40/83 (48%), Positives = 59/83 (71%)
Frame = +2 / +3
Query: 719 HEEAAEELPSKAKIFFSAAVEAWDAEFYVKVEDNINLDLAGLIEMLEGRRGSQGLYMGCM 898
H E L K K +F+ AV WDA+FYVKV+D++++++A L ++L + +YMGCM
Sbjct: 132 HVEGYLALSGKTKTYFATAVSLWDADFYVKVDDDVHVNIATLGQILSKQAWKPRVYMGCM 311
Query: 899 KSGMVISEEGQQWYEPEWWKFGD 967
KSG V+SE+G ++YEPE WKFG+
Sbjct: 312 KSGPVLSEKGVRYYEPEHWKFGE 380
Score = 49.6 bits (102), Expect(3) = 8e-040
Identities = 18/35 (51%), Positives = 26/35 (74%)
Frame = +2 / +3
Query: 977 YFRHASGSLFILSKNLARYININSASLQSYAHDDI 1081
YFRHA+G L+ +SK+LA YI+IN L Y ++D+
Sbjct: 393 YFRHATGQLYAISKDLATYISINKHVLHKYINEDV 497
Score = 48.3 bits (99), Expect(3) = 8e-040
Identities = 22/42 (52%), Positives = 29/42 (69%)
Frame = +2 / +3
Query: 599 EEKGVAIRFVIGRSANRGDSLDRNIDDENRQTKDFLILESHE 724
EEKGV IRFVIG SA G +DR I+ E+R+ DF+ ++ E
Sbjct: 15 EEKGVIIRFVIGHSAIAGGIIDRAIEAEDRKHGDFMKIDHVE 140
>gb|CK125868.1|CK125868 BES1824111A1e12 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010E1211 5-PRIME, mRNA sequence
Length = 375
Score = 162 bits (348), Expect = 5e-039
Identities = 69/119 (57%), Positives = 83/119 (69%)
Frame = +2 / +3
Query: 86 GAESPASEISAAMETLASAMRRENRRXXXXXXXXXXXXXXXGRVPLVMAFLSCLAWLYVA 265
G P+ EI+A ++ R R GRVPLVMAFL+CLAW+YVA
Sbjct: 18 GRSEPSEEIAAMETLASAMRRDNRRFKPPSSSSSSSAAASAGRVPLVMAFLACLAWVYVA 197
Query: 266 GRLWQDAQTRAILSGLLEKSSTSVPKVLSVEDKLRNLGCKAIGRKIVEAEMDLTKAKSE 442
GRLWQDAQTRAILSGLLEK++ ++PK +SV+DKLR+LGC IGRKI EAEMD+ KAKSE
Sbjct: 198 GRLWQDAQTRAILSGLLEKNTGNLPKEISVDDKLRDLGCTGIGRKIAEAEMDIAKAKSE 374
Score = 131 bits (281), Expect(2) = 1e-036
Identities = 58/78 (74%), Positives = 63/78 (80%)
Frame = -1 / -3
Query: 441 SLLALVRSISASTIFLPIALHPRFLSLSSTESTFGTLVELFSRRPDRIARVCASCHKRPA 262
SL AL SISAS IFLPI +HPR LSLSSTE + G L FSRRP+R+ARVCASCH RPA
Sbjct: 373 SLFALAMSISASAIFLPIPVHPRSLSLSSTEISLGRLPVFFSRRPERMARVCASCHSRPA 194
Query: 261 T*SQARQERKAMTRGTLP 208
T +QARQ RKAMTRGTLP
Sbjct: 193 TYTQARQARKAMTRGTLP 140
Score = 120 bits (256), Expect(2) = 2e-031
Identities = 52/78 (66%), Positives = 59/78 (75%)
Frame = -3 / -2
Query: 442 LALGLGQVHLRLNNFPSNRLAPKVPKLVLHREHLRDAGRALLEEAGQDRPGLRVLPQTAG 263
LAL LG VHLRL + P N A +VP+LV+HR+ L + LLEEAG+D PGLRVLP AG
Sbjct: 374 LALRLGNVHLRLGDLPPNPSASEVPQLVVHRDLLGEIAGVLLEEAGEDGPGLRVLP*PAG 195
Query: 262 DVEPGEAGEEGHDERDPA 209
DV PGEAGEEGHDE DPA
Sbjct: 194 DVHPGEAGEEGHDEGDPA 141
Score = 81.3 bits (171), Expect(2) = 3e-028
Identities = 34/56 (60%), Positives = 41/56 (73%)
Frame = +1 / +2
Query: 274 VAGRADPGDPVRPPREELYQRPEGALGGGQA*EPWVQGDWKENC*GGDGPDQGQER 441
+AGRADPG P+RPPREE Q P+G LGG QA P + DW+E+ GGDG QG+ER
Sbjct: 206 MAGRADPGHPLRPPREEHRQSPQGDLGGRQAEGPRMHWDWEEDRRGGDGHCQGEER 373
Score = 64.8 bits (135), Expect(2) = 3e-028
Identities = 27/40 (67%), Positives = 31/40 (77%)
Frame = +1 / +2
Query: 88 RREPSVGDLRRHGDAGQRHAAREPPVQGPALLLCFLRPRL 207
R+ +VG RRHGDAGQRHAAR+PP+Q P LLL LR RL
Sbjct: 17 RQVGAVGGDRRHGDAGQRHAARQPPLQAPVLLLLLLRRRL 136
Score = 42.8 bits (87), Expect(2) = 1e-036
Identities = 17/20 (85%), Positives = 18/20 (90%)
Frame = -3 / -2
Query: 160 AVLAPHGAGQRLHGGGDLRR 101
AV+APHGAGQRLHGG LRR
Sbjct: 89 AVVAPHGAGQRLHGGDLLRR 30
Score = 36.8 bits (74), Expect(2) = 2e-031
Identities = 18/22 (81%), Positives = 18/22 (81%)
Frame = -1 / -3
Query: 168 LDRRFSRRMALASVSMAAEISD 103
L RR SRRMALASVSMAA SD
Sbjct: 97 LKRRLSRRMALASVSMAAISSD 32
>gb|BJ469249.1|BJ469249 BJ469249 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal13n22 5', mRNA sequence
Length = 321
Score = 113 bits (241), Expect = 3e-024
Identities = 45/48 (93%), Positives = 46/48 (95%)
Frame = +2 / +1
Query: 1031 YININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCCSSSRQEKICS 1174
YININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCC SS QEK+CS
Sbjct: 4 YININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCCLSSVQEKVCS 147
Score = 105 bits (223), Expect = 8e-022
Identities = 47/59 (79%), Positives = 49/59 (83%)
Frame = -1 / -2
Query: 1206 RPAATPSAQALEQIFSCLELLQHNRSSST*VAFSPIIHEPTDISSCA*LCSDALFMLM* 1030
R AA PSA +E FSC EL QH RSSST*VA SPIIHEPTD+SSCA*LCSDALFMLM*
Sbjct: 179 RGAAGPSAHPMEHTFSCTELRQHRRSSST*VALSPIIHEPTDMSSCA*LCSDALFMLM* 3
>gb|BJ474454.1|BJ474454 BJ474454 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal13n22 3', mRNA sequence
Length = 256
Score = 113 bits (241), Expect = 3e-024
Identities = 45/48 (93%), Positives = 46/48 (95%)
Frame = +2 / -1
Query: 1031 YININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCCSSSRQEKICS 1174
YININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCC SS QEK+CS
Sbjct: 253 YININSASLQSYAHDDISVGSWMMGLNATYVDDDRLCCLSSVQEKVCS 110
Score = 105 bits (223), Expect = 8e-022
Identities = 47/59 (79%), Positives = 49/59 (83%)
Frame = -1 / +3
Query: 1206 RPAATPSAQALEQIFSCLELLQHNRSSST*VAFSPIIHEPTDISSCA*LCSDALFMLM* 1030
R AA PSA +E FSC EL QH RSSST*VA SPIIHEPTD+SSCA*LCSDALFMLM*
Sbjct: 78 RGAAGPSAHPMEHTFSCTELRQHRRSSST*VALSPIIHEPTDMSSCA*LCSDALFMLM* 254
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 601,232,305
Number of Sequences: 312970
Number of extensions: 9967158
Number of successful extensions: 114397
Number of sequences better than 1.0e-020: 26
length of database: 58,378,179
effective HSP length: 53
effective length of database: 41,790,769
effective search space used: 16967052214
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)