TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2404858.2.1
(958 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BF255425.2|BF255425 HVSMEf0006N05f Hordeum vulgare seedl... 149 6e-052
gb|BG299290.1|BG299290 HVSMEa0019D17f Hordeum vulgare seedl... 145 1e-046
gb|BG367721.1|BG367721 HVSMEi0013G05f Hordeum vulgare 20 DA... 52 1e-025
gb|BF618753.2|BF618753 HVSMEc0007K17f Hordeum vulgare seedl... 57 1e-024
gb|CA020535.1|CA020535 HZ36J17r HZ Hordeum vulgare subsp. v... 52 4e-021
>gb|BF255425.2|BF255425 HVSMEf0006N05f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0006N05f, mRNA sequence
Length = 587
Score = 149 bits (321), Expect(2) = 6e-052
Identities = 61/91 (67%), Positives = 70/91 (76%)
Frame = +1 / +2
Query: 1 RVCADLGAGAASYVHCDVTSEXXXXXXXXXXXXTFGALDIMFNNAGVSGPACYSVRESGK 180
R+CA+LG A+SYV CDVTSE FG LDIMFNNAG+ G AC+S+R+S K
Sbjct: 158 RLCAELGPAASSYVRCDVTSEEDVAAAVDHTVARFGKLDIMFNNAGIGGAACHSIRDSTK 337
Query: 181 ADFERVLAVNLVGPFLGTKHAARVMVPSRRG 273
DFERVLAVNLVGPFLGTKHAARVMVP+R+G
Sbjct: 338 EDFERVLAVNLVGPFLGTKHAARVMVPARQG 430
Score = 111 bits (236), Expect(2) = 1e-043
Identities = 54/92 (58%), Positives = 57/92 (61%)
Frame = -3 / -3
Query: 281 MQPPRREGTITRAACLVPRNGPTRFTASTRSKSALPLSRTL*HAGPLTPALLNMMSSAPN 102
M P R GT+TRAACLVPR GPTR TASTRSKS+L LSR L HA P PALLNMMSS PN
Sbjct: 438 MMQPCRAGTMTRAACLVPRKGPTRLTASTRSKSSLVLSRMLWHAAPPMPALLNMMSSFPN 259
Query: 101 VXXXXXXXXXXXXXXXXSQWT*LAAPAPRSAQ 6
+ S T*L A P SAQ
Sbjct: 258 LATV*STAAATSSSLVTSHRT*LEAAGPSSAQ 163
Score = 85.8 bits (181), Expect(2) = 1e-043
Identities = 38/54 (70%), Positives = 39/54 (72%)
Frame = -3 / -3
Query: 428 AGETQFTRMPCWPSSAAAFSVRVTIARFAHAYAYDDTPVTAAATLDVPMMQPPR 267
AGE Q TR+PCWPSS AAFSV T AR AH A D TP TA A LDVPMMQP R
Sbjct: 582 AGEMQLTRIPCWPSSVAAFSVSPTRARLAHV*ACDATPPTADARLDVPMMQPCR 421
Score = 83.1 bits (175), Expect(2) = 1e-025
Identities = 36/57 (63%), Positives = 41/57 (71%)
Frame = +3 / +1
Query: 111 AGHHVQQRGRQRAGVLQRPGERQG*LRARAGRESGGPVPRHQARGAGDGALAPRWLH 281
AGHHVQQR +R GV Q PG+ QG LRARAGR+ G +P +QARGAG GA A R H
Sbjct: 268 AGHHVQQRRHRRRGVPQHPGQHQGGLRARAGRQPRGALPGYQARGAGHGARAARLHH 438
Score = 74.8 bits (157), Expect(2) = 6e-052
Identities = 31/54 (57%), Positives = 33/54 (61%)
Frame = +1 / +2
Query: 268 RGGCIIXXXXXXXXXXXXXXXXXXCAKRAMVTLTENAAAELGQHGIRVNCVSPA 429
R GCII CAKRA+V LTENAA ELGQHGIRVNC+SPA
Sbjct: 422 RQGCIIGTSSLASAVGGVASHAYTCAKRALVGLTENAATELGQHGIRVNCISPA 583
Score = 53.3 bits (110), Expect(2) = 1e-025
Identities = 25/52 (48%), Positives = 29/52 (55%)
Frame = +3 / +1
Query: 276 LHHRDVERGGGSDWXXXXXXXXXEARDGDSDGERGCGARPARHSRELRLPRG 431
LHHRDVE G G +AR G +DG+RG GA PA R+L LP G
Sbjct: 430 LHHRDVEPGVGGGRRGVARLHVRQARPGRADGKRGDGAWPAWDPRQLHLPGG 585
>gb|BG299290.1|BG299290 HVSMEa0019D17f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0019D17f, mRNA sequence
Length = 627
Score = 145 bits (312), Expect(2) = 1e-046
Identities = 60/91 (65%), Positives = 68/91 (74%)
Frame = +1 / +3
Query: 1 RVCADLGAGAASYVHCDVTSEXXXXXXXXXXXXTFGALDIMFNNAGVSGPACYSVRESGK 180
R+CA+LG A+SYV CD TSE FG LDIMFNNAG+ G AC+S R+S K
Sbjct: 177 RLCAELGPAASSYVRCDFTSEEDITAAVYHAVARFGKLDIMFNNAGIGGAACHSFRDSTK 356
Query: 181 ADFERVLAVNLVGPFLGTKHAARVMVPSRRG 273
DFERVLAVNLVGPFLGTKHAARVMVP+R+G
Sbjct: 357 EDFERVLAVNLVGPFLGTKHAARVMVPARQG 449
Score = 101 bits (215), Expect(2) = 3e-037
Identities = 45/61 (73%), Positives = 48/61 (78%)
Frame = -3 / -3
Query: 281 MQPPRREGTITRAACLVPRNGPTRFTASTRSKSALPLSRTL*HAGPLTPALLNMMSSAPN 102
M P R GT+TRAACLVPR GPTR TASTRSKS+L LSR L HA P PALLNMMSS PN
Sbjct: 457 MMQPCRAGTMTRAACLVPRKGPTRLTASTRSKSSLVLSRKLWHAAPPMPALLNMMSSFPN 278
Query: 101 V 99
+
Sbjct: 277 L 275
Score = 74.4 bits (156), Expect(2) = 3e-037
Identities = 33/60 (55%), Positives = 37/60 (61%)
Frame = -3 / -3
Query: 446 SGVATPAGETQFTRMPCWPSSAAAFSVRVTIARFAHAYAYDDTPVTAAATLDVPMMQPPR 267
+ V +GE TR+PCWPSS AA V T AR AH A+ P TA A LDVPMMQP R
Sbjct: 619 TSVTATSGEINLTRIPCWPSSVAAVFVSQTSARLAHV*AWXAKPPTADARLDVPMMQPCR 440
Score = 61.6 bits (128), Expect(2) = 1e-046
Identities = 27/58 (46%), Positives = 30/58 (51%)
Frame = +1 / +3
Query: 268 RGGCIIXXXXXXXXXXXXXXXXXXCAKRAMVTLTENAAAELGQHGIRVNCVSPAGVAT 441
R GCII CAKRA+V LT+ AA ELGQHGIRV +SP T
Sbjct: 441 RQGCIIGTSSLASAVGGLASHAYTCAKRALVWLTKTAATELGQHGIRVKLISPLVAVT 614
>gb|BG367721.1|BG367721 HVSMEi0013G05f Hordeum vulgare 20 DAP spike EST library HVcDNA0010
(20 DAP) Hordeum vulgare subsp. vulgare cDNA clone
HVSMEi0013G05f, mRNA sequence
Length = 785
Score = 51.9 bits (107), Expect(4) = 1e-025
Identities = 22/39 (56%), Positives = 26/39 (66%)
Frame = +1 / -2
Query: 511 LKGVGALRVHDVAAAVRFLASDDARYISGHNLFVDGGIS 627
L G L DVA +LASDDA Y+SGHNL VDGG++
Sbjct: 307 LLGKATLEPGDVADPAVYLASDDAEYVSGHNLVVDGGLT 191
Score = 51.0 bits (105), Expect(4) = 1e-025
Identities = 22/65 (33%), Positives = 33/65 (50%)
Frame = +1 / -2
Query: 268 RGGCIIXXXXXXXXXXXXXXXXXXCAKRAMVTLTENAAAELGQHGIRVNCVSPAGVATPL 447
R G I+ +K A++ + ++ AA+L Q+GIRVNC+SP V TP
Sbjct: 562 RTGSILCTASATGMMGGIAPQTYSVSKSAVIVIVKSMAAQLCQYGIRVNCISPFAVPTPF 383
Query: 448 TMRYM 462
T+ M
Sbjct: 382 TLDQM 368
Score = 35.9 bits (72), Expect(4) = 1e-025
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = +1 / -2
Query: 205 VNLVGPFLGTKHAARVMVPSRRGGCI 282
+N+ G G KHA+RVM+PSR G +
Sbjct: 622 INVRGVLAGIKHASRVMIPSRTGSIL 545
Score = 32.7 bits (65), Expect(4) = 1e-025
Identities = 17/52 (32%), Positives = 23/52 (44%)
Frame = +1 / -1
Query: 46 CDVTSEXXXXXXXXXXXXTFGALDIMFNNAGVSGPACYSVRESGKADFERVL 201
C+VT E LD+MFNN GV S+ + A F+RV+
Sbjct: 782 CNVTEESEASDGVDFTVSKHKRLDVMFNNVGVPCFTPPSIVDLDVAIFDRVM 627
>gb|BF618753.2|BF618753 HVSMEc0007K17f Hordeum vulgare seedling shoot EST library
HVcDNA0003 (Etiolated and unstressed) Hordeum vulgare
subsp. vulgare cDNA clone HVSMEc0007K17f, mRNA sequence
Length = 796
Score = 56.5 bits (117), Expect(4) = 1e-024
Identities = 25/58 (43%), Positives = 34/58 (58%)
Frame = +1 / +2
Query: 457 YMGLEAEALEQAMEGMANLKGVGALRVHDVAAAVRFLASDDARYISGHNLFVDGGISV 630
Y AE + +EG N L D+A A +LASD+A+Y++GHNL VDGG +V
Sbjct: 374 YPKASAEEHRRIVEGDINEMEGAVLEPDDIARAALYLASDEAKYVNGHNLVVDGGFTV 547
Score = 46.4 bits (95), Expect(4) = 1e-024
Identities = 19/34 (55%), Positives = 22/34 (64%)
Frame = +1 / +2
Query: 181 ADFERVLAVNLVGPFLGTKHAARVMVPSRRGGCI 282
ADF+ V+A+N G G KHAARVM P R G I
Sbjct: 86 ADFDAVMAINARGVLAGVKHAARVMAPRRSGSII 187
Score = 38.2 bits (77), Expect(4) = 1e-024
Identities = 14/40 (35%), Positives = 24/40 (60%)
Frame = +1 / +2
Query: 343 AKRAMVTLTENAAAELGQHGIRVNCVSPAGVATPLTMRYM 462
+K ++ + A E+ + G+RVN +SP + TPL MR +
Sbjct: 245 SKATVLGVVRAVAGEMARSGVRVNAISPNYIPTPLVMRIL 364
Score = 27.2 bits (53), Expect(4) = 1e-024
Identities = 10/14 (71%), Positives = 11/14 (78%)
Frame = +1 / +2
Query: 106 GALDIMFNNAGVSG 147
G LDIM NNAG+ G
Sbjct: 8 GKLDIMLNNAGIVG 49
>gb|CA020535.1|CA020535 HZ36J17r HZ Hordeum vulgare subsp. vulgare cDNA clone HZ36J17
5-PRIME, mRNA sequence
Length = 556
Score = 52.4 bits (108), Expect(3) = 4e-021
Identities = 22/48 (45%), Positives = 24/48 (50%)
Frame = +1 / +1
Query: 4 VCADLGAGAASYVHCDVTSEXXXXXXXXXXXXTFGALDIMFNNAGVSG 147
V A+LG AA Y CDVT E G LDIM NNAG+ G
Sbjct: 61 VAAELGPNAACYARCDVTDEAQVAAAVDLAVARHGKLDIMLNNAGIMG 204
Score = 46.4 bits (95), Expect(3) = 4e-021
Identities = 19/34 (55%), Positives = 22/34 (64%)
Frame = +1 / +1
Query: 181 ADFERVLAVNLVGPFLGTKHAARVMVPSRRGGCI 282
ADF+ V+A+N G G KHAARVM P R G I
Sbjct: 241 ADFDAVMAINARGVLAGVKHAARVMAPRRSGSII 342
Score = 40.0 bits (81), Expect(3) = 4e-021
Identities = 15/40 (37%), Positives = 25/40 (62%)
Frame = +1 / +1
Query: 343 AKRAMVTLTENAAAELGQHGIRVNCVSPAGVATPLTMRYM 462
+K A++ + A E+ + G+RVN +SP + TPL MR +
Sbjct: 400 SKAAVLGVVRAVAGEMARSGVRVNAISPNYIPTPLVMRIL 519
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 390,069,742
Number of Sequences: 312970
Number of extensions: 6114588
Number of successful extensions: 113087
Number of sequences better than 1.0e-020: 6
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 11199594574
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)