TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192481.2.2
(1053 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BM816845.1|BM816845 HB105B04_SK.ab1 HB Hordeum vulgare s... 124 3e-046
gb|BF621527.1|BF621527 HVSMEa0011E23f Hordeum vulgare seedl... 117 2e-045
gb|BJ460652.1|BJ460652 BJ460652 K. Sato unpublished cDNA li... 169 2e-043
gb|AV934980.1|AV934980 AV934980 K. Sato unpublished cDNA li... 146 6e-043
gb|BJ476097.1|BJ476097 BJ476097 K. Sato unpublished cDNA li... 146 6e-043
gb|AL501651.1|AL501651 AL501651 Hordeum vulgare Barke roots... 111 1e-042
gb|AV919848.1|AV919848 AV919848 K. Sato unpublished cDNA li... 147 3e-040
gb|AV935828.1|AV935828 AV935828 K. Sato unpublished cDNA li... 147 3e-040
gb|BQ739853.1|BQ739853 HB04A05 HB Hordeum vulgare subsp. vu... 97 3e-038
gb|CA012646.1|CA012646 HT06A10r HT Hordeum vulgare subsp. v... 96 5e-038
gb|BU996421.1|BU996421 HM13I21r HM Hordeum vulgare subsp. v... 146 2e-037
gb|BE602191.1|BE602191 HVSMEh0102O23f Hordeum vulgare 5-45 ... 146 8e-037
gb|AV933386.1|AV933386 AV933386 K. Sato unpublished cDNA li... 88 1e-035
gb|AV933542.1|AV933542 AV933542 K. Sato unpublished cDNA li... 88 1e-035
gb|BE214410.3|BE214410 HV_CEb0003G01f Hordeum vulgare seedl... 66 4e-035
gb|BF260055.2|BF260055 HVSMEf0020P20f Hordeum vulgare seedl... 82 9e-035
gb|AV921854.1|AV921854 AV921854 K. Sato unpublished cDNA li... 147 1e-034
gb|AV936314.1|AV936314 AV936314 K. Sato unpublished cDNA li... 147 1e-034
gb|BQ663284.1|BQ663284 HU01O19w HU Hordeum vulgare subsp. v... 147 1e-034
gb|AV935981.1|AV935981 AV935981 K. Sato unpublished cDNA li... 146 2e-034
gb|BQ464865.1|BQ464865 HU01O19T HU Hordeum vulgare subsp. v... 84 1e-033
gb|AV915236.1|AV915236 AV915236 K. Sato unpublished cDNA li... 79 5e-033
gb|AV934062.1|AV934062 AV934062 K. Sato unpublished cDNA li... 79 5e-033
gb|BF621761.2|BF621761 HVSMEa0012H13f Hordeum vulgare seedl... 73 3e-032
gb|BJ475625.1|BJ475625 BJ475625 K. Sato unpublished cDNA li... 127 7e-029
gb|BF258180.2|BF258180 HVSMEf0015A11f Hordeum vulgare seedl... 63 2e-028
gb|CA017090.1|CA017090 HV14O23u HV Hordeum vulgare subsp. v... 124 9e-028
gb|CA011285.1|CA011285 HT06A10u HT Hordeum vulgare subsp. v... 123 2e-027
gb|BQ662063.1|BQ662063 HR01G10u HR Hordeum vulgare subsp. v... 122 5e-027
gb|BG300201.1|BG300201 HVSMEb0010O04f Hordeum vulgare seedl... 111 1e-026
gb|BM375339.2|BM375339 EBem06_SQ003_A21_R embryo, 21 DPA, n... 62 2e-026
gb|CK122919.1|CK122919 BES1824101e05 BES1824 Hordeum vulgar... 115 4e-025
gb|BE214395.2|BE214395 HV_CEb0003F06f Hordeum vulgare seedl... 58 3e-024
gb|AV932136.1|AV932136 AV932136 K. Sato unpublished cDNA li... 61 2e-023
gb|BJ472055.1|BJ472055 BJ472055 K. Sato unpublished cDNA li... 61 2e-023
gb|AV932651.1|AV932651 AV932651 K. Sato unpublished cDNA li... 61 2e-023
gb|BQ467934.1|BQ467934 HR01G10r HR Hordeum vulgare subsp. v... 61 2e-023
gb|AJ432736.1|AJ432736 AJ432736 S00002 Hordeum vulgare subs... 107 1e-022
gb|AV912065.1|AV912065 AV912065 K. Sato unpublished cDNA li... 103 2e-021
gb|CV059100.1|CV059100 BNEL44d1 Barley EST endosperm librar... 43 8e-021
>gb|BM816845.1|BM816845 HB105B04_SK.ab1 HB Hordeum vulgare subsp. vulgare cDNA clone
HB105B04_SK.ab1 similar to (AF370531) Unknown protein
[Arabidopsis thaliana], mRNA sequence
Length = 887
Score = 124 bits (265), Expect(3) = 3e-046
Identities = 51/125 (40%), Positives = 72/125 (57%)
Frame = -2 / +2
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+
Sbjct: 419 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAE 598
Query: 416 NDTTTPPKEVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMV 237
D +PP+ V F VL + + KIF GV HG+ AVK+AE AL M+
Sbjct: 599 TDVMSPPELVKEFEQVLSSNSGIAHXVKIFPGVSHGWTVXIYGEXAAAVKSAEGALTDMI 778
Query: 236 NWFNK 222
+W N+
Sbjct: 779 DWVNR 793
Score = 97.7 bits (207), Expect(2) = 2e-022
Identities = 52/119 (43%), Positives = 62/119 (52%)
Frame = +1 / -3
Query: 214 LRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRYTS 393
L LL Q M RA SA+ TA L +P TP KIL * L TC+N T+
Sbjct: 801 LXVLLTQSIMSVRAPSALFTAAAXSPYXLTVHP*ETPGKILTX*AIPELDDRTCSNSLTN 622
Query: 394 FGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ*PPTAT 570
GG + S AP ++GHFTSF+SS VT GC+ SL F S +F PQQ*P T
Sbjct: 621 SGGDITSVSAPSTAMGHFTSFISSTVTKEGCDITAVWISLAFANSATTFAPQQ*PAAPT 445
Score = 61.6 bits (128), Expect(3) = 3e-046
Identities = 29/59 (49%), Positives = 34/59 (57%)
Frame = -2 / +2
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG++ VFG+ AP LR+IADKVA +GYFVVVPDFL GD
Sbjct: 146 VVDSFGGIKAYVAGAQESKAAVVLISDVFGFGAPNLRKIADKVAASGYFVVVPDFLHGD 322
Score = 38.6 bits (78), Expect(3) = 3e-046
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = -2 / +2
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++G S
Sbjct: 362 WIKEHTAGKAFEEAKPVIAALKEQGAS 442
Score = 31.3 bits (62), Expect(2) = 2e-022
Identities = 15/22 (68%), Positives = 15/22 (68%)
Frame = +1 / -3
Query: 715 YPALATLSAICLSNGASYPKTS 780
YP ATLSAI L GA PKTS
Sbjct: 288 YPDAATLSAIFLRFGAPNPKTS 223
>gb|BF621527.1|BF621527 HVSMEa0011E23f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0011E23f, mRNA sequence
Length = 844
Score = 117 bits (251), Expect(3) = 2e-045
Identities = 48/120 (40%), Positives = 73/120 (60%)
Frame = -2 / +1
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPK 393
+V GYCWG K+ E+ K +++ A +SHP VT +D+KEVK PI +LGA+ D T+PP+
Sbjct: 439 TVGAAGYCWGAKVVAELAKANEIHAAVMSHPSFVTVEDIKEVKCPIAVLGAEIDKTSPPE 618
Query: 392 EVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN 213
V +F VL + ++ KIF G HG+ RY + D A K A + A+ + +K+L+
Sbjct: 619 LVKQFEQVLSSNSGIAHFVKIFPGGSHGWTVRYKSEDAGAGKNA*ESPAHWIELVSKNLH 798
Score = 61.1 bits (127), Expect(3) = 2e-045
Identities = 29/59 (49%), Positives = 33/59 (55%)
Frame = -2 / +1
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG+ VFG+EAP LR+IADKVA +GY VVVPDFL GD
Sbjct: 142 VVDSFGGITAYVSGARESKAAVVLISDVFGFEAPNLRKIADKVASSGYLVVVPDFLHGD 318
Score = 42.8 bits (87), Expect(3) = 2e-045
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = -2 / +1
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
WL+ H+P KA E+AKP+ AALK++G S
Sbjct: 358 WLKEHAPGKAFEEAKPVIAALKEQGVS 438
>gb|BJ460652.1|BJ460652 BJ460652 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak41l02 3', mRNA sequence
Length = 742
Score = 169 bits (363), Expect(2) = 2e-043
Identities = 67/127 (52%), Positives = 89/127 (70%)
Frame = -2 / -3
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A K+ V VGGYCWG K++VE+ K+ +++ V +SHP VT DDMKEVK PIEILGA+
Sbjct: 599 ALKKQGVSEVGVGGYCWGAKVAVELSKSEEIQVVVISHPSQVTVDDMKEVKHPIEILGAE 420
Query: 416 NDTTTPPKEVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMV 237
D +PP V++F L + +++ + KIF GV HGFACRY+ D FAVK AE A A M+
Sbjct: 419 LDQASPPPIVHQFEQALDQNNKIDRFVKIFPGVAHGFACRYDANDAFAVKNAEEARADML 240
Query: 236 NWFNKHL 216
+WFNK+L
Sbjct: 239 SWFNKYL 219
Score = 71.6 bits (150), Expect(2) = 1e-021
Identities = 36/66 (54%), Positives = 40/66 (60%)
Frame = +1 / +1
Query: 373 CTNRYTSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ 552
C+N T GG S APRIS+G TSFMSS VT GCE T S F +ST +F PQQ
Sbjct: 376 CSNWCTIGGGEA*SSSAPRISMGCLTSFMSSTVTCEGCEMTTT*ISSDFDSSTATFAPQQ 555
Query: 553 *PPTAT 570
PPT T
Sbjct: 556 YPPTPT 573
Score = 51.9 bits (107), Expect(2) = 1e-021
Identities = 25/39 (64%), Positives = 28/39 (71%)
Frame = +1 / +1
Query: 217 RCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKI 333
R LLNQL+M ARA SA LTA S+ YLQA P +TP KI
Sbjct: 220 RYLLNQLSMSARASSAFLTANASLASYLQAKPWATPGKI 336
Score = 27.2 bits (53), Expect(2) = 2e-043
Identities = 13/20 (65%), Positives = 14/20 (70%)
Frame = -2 / -3
Query: 629 VKAAEDAKPLFAALKKEGKS 570
V AAE K L AALKK+G S
Sbjct: 635 VVAAEKTKVLIAALKKQGVS 576
>gb|AV934980.1|AV934980 AV934980 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal2g20 3', mRNA sequence
Length = 701
Score = 146 bits (313), Expect(3) = 6e-043
Identities = 57/122 (46%), Positives = 83/122 (68%)
Frame = -2 / -3
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPK 393
+V GYCWG K+ E+ K +++ A +SHP VT +D+KEVK PI +LGA+ D T+PP+
Sbjct: 549 TVGAAGYCWGAKVVAELAKANEIHAAVMSHPSFVTVEDIKEVKCPIAVLGAEIDKTSPPE 370
Query: 392 EVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN 213
V +F VL + ++ KIF GV HG+ RY + D AVK+AE AL++M++WF+K+L
Sbjct: 369 LVKQFEQVLSSNSGIAHFVKIFPGVSHGWTVRYKSEDAGAVKSAEEALSHMIDWFSKNLQ 190
Query: 212 *I 207
*I
Sbjct: 189 *I 184
Score = 106 bits (226), Expect = 2e-022
Identities = 56/121 (46%), Positives = 69/121 (57%)
Frame = +1 / +2
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
I R LLNQ + RA SA+ TA S +LYL +P TP IL * L TC+N
Sbjct: 185 IHCRFLLNQSIICDRASSALFTAPASSLLYLTVHP*ETPGNILTK*AIPELEDRTCSNCL 364
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ*PPTA 567
T+ GG V+S AP ++GHFTS +SS VT GC+ A SL F +S +F PQQ*P
Sbjct: 365 TNSGGDVLSISAPSTAMGHFTSLISSTVTKEGCDITAAWISLAFASSATTFAPQQ*PAAP 544
Query: 568 T 570
T
Sbjct: 545 T 547
Score = 42.8 bits (87), Expect(3) = 6e-043
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = -2 / -3
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
WL+ H+P KA E+AKP+ AALK++G S
Sbjct: 630 WLKEHAPGKAFEEAKPVIAALKEQGVS 550
Score = 24.0 bits (46), Expect(3) = 6e-043
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = -2 / -3
Query: 710 VVVPDFLKGD 681
VVVPDFL GD
Sbjct: 699 VVVPDFLHGD 670
>gb|BJ476097.1|BJ476097 BJ476097 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal31o24 3', mRNA sequence
Length = 690
Score = 146 bits (313), Expect(3) = 6e-043
Identities = 57/122 (46%), Positives = 83/122 (68%)
Frame = -2 / -2
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPK 393
+V GYCWG K+ E+ K +++ A +SHP VT +D+KEVK PI +LGA+ D T+PP+
Sbjct: 539 TVGAAGYCWGAKVVAELAKANEIHAAVMSHPSFVTVEDIKEVKCPIAVLGAEIDKTSPPE 360
Query: 392 EVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN 213
V +F VL + ++ KIF GV HG+ RY + D AVK+AE AL++M++WF+K+L
Sbjct: 359 LVKQFEQVLSSNSGIAHFVKIFPGVSHGWTVRYKSEDAGAVKSAEEALSHMIDWFSKNLQ 180
Query: 212 *I 207
*I
Sbjct: 179 *I 174
Score = 106 bits (226), Expect = 2e-022
Identities = 56/121 (46%), Positives = 69/121 (57%)
Frame = +1 / +1
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
I R LLNQ + RA SA+ TA S +LYL +P TP IL * L TC+N
Sbjct: 175 IHCRFLLNQSIICDRASSALFTAPASSLLYLTVHP*ETPGNILTK*AIPELEDRTCSNCL 354
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ*PPTA 567
T+ GG V+S AP ++GHFTS +SS VT GC+ A SL F +S +F PQQ*P
Sbjct: 355 TNSGGDVLSISAPSTAMGHFTSLISSTVTKEGCDITAAWISLAFASSATTFAPQQ*PAAP 534
Query: 568 T 570
T
Sbjct: 535 T 537
Score = 42.8 bits (87), Expect(3) = 6e-043
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = -2 / -2
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
WL+ H+P KA E+AKP+ AALK++G S
Sbjct: 620 WLKEHAPGKAFEEAKPVIAALKEQGVS 540
Score = 24.0 bits (46), Expect(3) = 6e-043
Identities = 9/10 (90%), Positives = 9/10 (90%)
Frame = -2 / -2
Query: 710 VVVPDFLKGD 681
VVVPDFL GD
Sbjct: 689 VVVPDFLHGD 660
>gb|AL501651.1|AL501651 AL501651 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW04O13u 3', mRNA sequence
Length = 490
Score = 111 bits (238), Expect(2) = 1e-042
Identities = 44/77 (57%), Positives = 55/77 (71%)
Frame = -2 / -3
Query: 440 PIEILGAQNDTTTPPKEVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTA 261
P +GA+ND TPP V + V L +R+++ YYAKIF V HGFACRYN T+PFAVKTA
Sbjct: 353 P*RFIGAENDAVTPPALVDQLVETLHQRNQIQYYAKIFPEVAHGFACRYNVTNPFAVKTA 174
Query: 260 ETALAYMVNWFNKHLN* 210
E AL M+ WF+K+L *
Sbjct: 173 EQALGLMLGWFHKYLK* 123
Score = 81.7 bits (172), Expect(2) = 1e-042
Identities = 30/45 (66%), Positives = 38/45 (84%)
Frame = -2 / -2
Query: 563 VGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEI 429
+G YCWGGK +VEV KTS+V+A+ +SHP +VT DDMKE+K PIEI
Sbjct: 477 LGXYCWGGKFAVEVAKTSEVEAIVISHPSAVTTDDMKEIKSPIEI 343
>gb|AV919848.1|AV919848 AV919848 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags7o08 3', mRNA sequence
Length = 671
Score = 147 bits (315), Expect(2) = 3e-040
Identities = 59/129 (45%), Positives = 81/129 (62%)
Frame = -2 / -1
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+
Sbjct: 563 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAE 384
Query: 416 NDTTTPPKEVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMV 237
D +PP+ V F VL + ++ KIF GV HG+ RYN D AVK+AE AL M+
Sbjct: 383 TDVMSPPELVKEFEQVLSSNSGIAHFVKIFPGVSHGWTVRYNGEDAAAVKSAEEALTDMI 204
Query: 236 NWFNKHLN* 210
+WFNK+L *
Sbjct: 203 DWFNKNLK* 177
Score = 111 bits (238), Expect = 5e-024
Identities = 57/121 (47%), Positives = 68/121 (56%)
Frame = +1 / +1
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
+ LR LLNQ M RA SA+ TA S LYL +P TP KIL * L TC+N
Sbjct: 175 VHLRFLLNQSIMSVRASSALFTAAASSPLYLTVHP*ETPGKILTK*AIPELDDRTCSNSL 354
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ*PPTA 567
T+ GG + S AP ++GHFTSF+SS VT GC+ SL F S +F PQQ*P
Sbjct: 355 TNSGGDITSVSAPSTAMGHFTSFISSTVTKEGCDITAVWISLAFANSATTFAPQQ*PAAP 534
Query: 568 T 570
T
Sbjct: 535 T 537
Score = 38.6 bits (78), Expect(2) = 3e-040
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = -2 / -1
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++G S
Sbjct: 620 WIKEHTAGKAFEEAKPVIAALKEQGAS 540
>gb|AV935828.1|AV935828 AV935828 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal6j21 3', mRNA sequence
Length = 648
Score = 147 bits (315), Expect(2) = 3e-040
Identities = 59/129 (45%), Positives = 81/129 (62%)
Frame = -2 / -3
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+
Sbjct: 574 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAE 395
Query: 416 NDTTTPPKEVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMV 237
D +PP+ V F VL + ++ KIF GV HG+ RYN D AVK+AE AL M+
Sbjct: 394 TDVMSPPELVKEFEQVLSSNSGIAHFVKIFPGVSHGWTVRYNGEDAAAVKSAEEALTDMI 215
Query: 236 NWFNKHLN* 210
+WFNK+L *
Sbjct: 214 DWFNKNLK* 188
Score = 111 bits (238), Expect = 5e-024
Identities = 57/121 (47%), Positives = 68/121 (56%)
Frame = +1 / +3
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
+ LR LLNQ M RA SA+ TA S LYL +P TP KIL * L TC+N
Sbjct: 186 VHLRFLLNQSIMSVRASSALFTAAASSPLYLTVHP*ETPGKILTK*AIPELDDRTCSNSL 365
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ*PPTA 567
T+ GG + S AP ++GHFTSF+SS VT GC+ SL F S +F PQQ*P
Sbjct: 366 TNSGGDITSVSAPSTAMGHFTSFISSTVTKEGCDITAVWISLAFANSATTFAPQQ*PAAP 545
Query: 568 T 570
T
Sbjct: 546 T 548
Score = 38.6 bits (78), Expect(2) = 3e-040
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = -2 / -3
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++G S
Sbjct: 631 WIKEHTAGKAFEEAKPVIAALKEQGAS 551
>gb|BQ739853.1|BQ739853 HB04A05 HB Hordeum vulgare subsp. vulgare cDNA clone HB04A05
similar to NP_566732.1| (NM_113263) expressed protein
[Arabidopsis thaliana] gi|9294516|dbj|BAB02778.1|
(AB023036) contains similarity to
endo-1,3-1,4-beta-D-glucanase gene_id:MDB19.8..., mRNA
sequence
Length = 638
Score = 97.3 bits (206), Expect(3) = 3e-038
Identities = 38/96 (39%), Positives = 55/96 (57%)
Frame = -2 / +1
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+ EVK PI +LG+
Sbjct: 349 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIXEVKCPIAVLGS* 528
Query: 416 NDTTTPPKEVYRFVHVLRERHEVPYYAKIFQGVEHG 309
D +PP+ V F VL + ++ KIF G HG
Sbjct: 529 TDVMSPPELVKEFEQVLSSNSXIAHFVKIFPGGSHG 636
Score = 61.6 bits (128), Expect(3) = 3e-038
Identities = 29/59 (49%), Positives = 34/59 (57%)
Frame = -2 / +1
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG++ VFG+ AP LR+IADKVA +GYFVVVPDFL GD
Sbjct: 76 VVDSFGGIKAYVAGAQESKAAVVLISDVFGFGAPNLRKIADKVAASGYFVVVPDFLHGD 252
Score = 38.6 bits (78), Expect(3) = 3e-038
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = -2 / +1
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++G S
Sbjct: 292 WIKEHTAGKAFEEAKPVIAALKEQGAS 372
>gb|CA012646.1|CA012646 HT06A10r HT Hordeum vulgare subsp. vulgare cDNA clone HT06A10
5-PRIME, mRNA sequence
Length = 651
Score = 96.4 bits (204), Expect(3) = 5e-038
Identities = 37/90 (41%), Positives = 54/90 (60%)
Frame = -2 / +1
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+
Sbjct: 382 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAE 561
Query: 416 NDTTTPPKEVYRFVHVLRERHEVPYYAKIF 327
D +PP+ V F VL + ++ KIF
Sbjct: 562 TDVMSPPELVKEFEQVLSSNSGIAHFVKIF 651
Score = 61.6 bits (128), Expect(3) = 5e-038
Identities = 29/59 (49%), Positives = 34/59 (57%)
Frame = -2 / +1
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG++ VFG+ AP LR+IADKVA +GYFVVVPDFL GD
Sbjct: 109 VVDSFGGIKAYVAGAQESKAAVVLISDVFGFGAPNLRKIADKVAASGYFVVVPDFLHGD 285
Score = 38.6 bits (78), Expect(3) = 5e-038
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = -2 / +1
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++G S
Sbjct: 325 WIKEHTAGKAFEEAKPVIAALKEQGAS 405
>gb|BU996421.1|BU996421 HM13I21r HM Hordeum vulgare subsp. vulgare cDNA clone HM13I21
5-PRIME, mRNA sequence
Length = 641
Score = 146 bits (313), Expect(2) = 2e-037
Identities = 57/122 (46%), Positives = 83/122 (68%)
Frame = -2 / +3
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPK 393
+V GYCWG K+ E+ K +++ A +SHP VT +D+KEVK PI +LGA+ D T+PP+
Sbjct: 222 TVGAAGYCWGAKVVAELAKANEIHAAVMSHPSFVTVEDIKEVKCPIAVLGAEIDKTSPPE 401
Query: 392 EVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN 213
V +F VL + ++ KIF GV HG+ RY + D AVK+AE AL++M++WF+K+L
Sbjct: 402 LVKQFEQVLSSNSGIAHFVKIFPGVSHGWTVRYKSEDAGAVKSAEEALSHMIDWFSKNLQ 581
Query: 212 *I 207
*I
Sbjct: 582 *I 587
Score = 106 bits (226), Expect = 2e-022
Identities = 56/121 (46%), Positives = 69/121 (57%)
Frame = +1 / -2
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
I R LLNQ + RA SA+ TA S +LYL +P TP IL * L TC+N
Sbjct: 586 IHCRFLLNQSIICDRASSALFTAPASSLLYLTVHP*ETPGNILTK*AIPELEDRTCSNCL 407
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ*PPTA 567
T+ GG V+S AP ++GHFTS +SS VT GC+ A SL F +S +F PQQ*P
Sbjct: 406 TNSGGDVLSISAPSTAMGHFTSLISSTVTKEGCDITAAWISLAFASSATTFAPQQ*PAAP 227
Query: 568 T 570
T
Sbjct: 226 T 224
Score = 29.9 bits (59), Expect(2) = 2e-037
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = -2 / +3
Query: 626 KAAEDAKPLFAALKKEGKS 570
KA E+AKP+ AALK++G S
Sbjct: 165 KAFEEAKPVIAALKEQGVS 221
>gb|BE602191.1|BE602191 HVSMEh0102O23f Hordeum vulgare 5-45 DAP spike EST library
HVcDNA0009 (5 to 45 DAP) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEh0102O23f, mRNA sequence
Length = 733
Score = 146 bits (314), Expect(2) = 8e-037
Identities = 58/121 (47%), Positives = 78/121 (64%)
Frame = -2 / -3
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPK 393
SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+ D +PP+
Sbjct: 677 SVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAETDVMSPPE 498
Query: 392 EVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN 213
V F VL + ++ KIF GV HG+ RYN D AVK+AE AL M++WFNK+L
Sbjct: 497 LVKEFEQVLSSNSGIAHFVKIFPGVSHGWTVRYNGEDAAAVKSAEEALTDMIDWFNKNLK 318
Query: 212 * 210
*
Sbjct: 317 * 315
Score = 111 bits (238), Expect = 5e-024
Identities = 57/121 (47%), Positives = 68/121 (56%)
Frame = +1 / +1
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
+ LR LLNQ M RA SA+ TA S LYL +P TP KIL * L TC+N
Sbjct: 313 VHLRFLLNQSIMSVRASSALFTAAASSPLYLTVHP*ETPGKILTK*AIPELDDRTCSNSL 492
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ*PPTA 567
T+ GG + S AP ++GHFTSF+SS VT GC+ SL F S +F PQQ*P
Sbjct: 493 TNSGGDITSVSAPSTAMGHFTSFISSTVTKEGCDITAVWISLAFANSATTFAPQQ*PAAP 672
Query: 568 T 570
T
Sbjct: 673 T 675
Score = 27.6 bits (54), Expect(2) = 8e-037
Identities = 11/18 (61%), Positives = 14/18 (77%)
Frame = -2 / -3
Query: 623 AAEDAKPLFAALKKEGKS 570
A E+AKP+ AALK +G S
Sbjct: 731 AFEEAKPVIAALKNQGAS 678
>gb|AV933386.1|AV933386 AV933386 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal6j21 5', mRNA sequence
Length = 599
Score = 88.1 bits (186), Expect(3) = 1e-035
Identities = 34/77 (44%), Positives = 48/77 (62%)
Frame = -2 / +2
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+
Sbjct: 356 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAE 535
Query: 416 NDTTTPPKEVYRFVHVL 366
D +PP+ V F VL
Sbjct: 536 TDVMSPPELVKEFEQVL 586
Score = 61.6 bits (128), Expect(3) = 1e-035
Identities = 29/59 (49%), Positives = 34/59 (57%)
Frame = -2 / +2
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG++ VFG+ AP LR+IADKVA +GYFVVVPDFL GD
Sbjct: 83 VVDSFGGIKAYVAGAQESKAAVVLISDVFGFGAPNLRKIADKVAASGYFVVVPDFLHGD 259
Score = 38.6 bits (78), Expect(3) = 1e-035
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = -2 / +2
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++G S
Sbjct: 299 WIKEHTAGKAFEEAKPVIAALKEQGAS 379
>gb|AV933542.1|AV933542 AV933542 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal7f16 5', mRNA sequence
Length = 590
Score = 88.1 bits (186), Expect(3) = 1e-035
Identities = 34/77 (44%), Positives = 48/77 (62%)
Frame = -2 / +1
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+
Sbjct: 352 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAE 531
Query: 416 NDTTTPPKEVYRFVHVL 366
D +PP+ V F VL
Sbjct: 532 TDVMSPPELVKEFEQVL 582
Score = 61.6 bits (128), Expect(3) = 1e-035
Identities = 29/59 (49%), Positives = 34/59 (57%)
Frame = -2 / +1
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG++ VFG+ AP LR+IADKVA +GYFVVVPDFL GD
Sbjct: 79 VVDSFGGIKAYVAGAQESKAAVVLISDVFGFGAPNLRKIADKVAASGYFVVVPDFLHGD 255
Score = 38.6 bits (78), Expect(3) = 1e-035
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = -2 / +1
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++G S
Sbjct: 295 WIKEHTAGKAFEEAKPVIAALKEQGAS 375
>gb|BE214410.3|BE214410 HV_CEb0003G01f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0003G01f, mRNA sequence
Length = 712
Score = 65.7 bits (137), Expect(5) = 4e-035
Identities = 25/62 (40%), Positives = 38/62 (61%)
Frame = -2 / +1
Query: 518 KTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPKEVYRFVHVLRERHEVPYY 339
K ++++ +SHP VT DD+KEVK PI +LGA+ D +PP+ V F VL + ++
Sbjct: 466 KANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAETDVMSPPELVKEFEQVLSSNSXIAHF 645
Query: 338 AK 333
K
Sbjct: 646 VK 651
Score = 61.6 bits (128), Expect(5) = 4e-035
Identities = 29/59 (49%), Positives = 34/59 (57%)
Frame = -2 / +3
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG++ VFG+ AP LR+IADKVA +GYFVVVPDFL GD
Sbjct: 114 VVDSFGGIKAYVAGAQESKAAVVLISDVFGFGAPNLRKIADKVAASGYFVVVPDFLHGD 290
Score = 38.6 bits (78), Expect(5) = 4e-035
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = -2 / +3
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++G S
Sbjct: 330 WIKEHTAGKAFEEAKPVIAALKEQGAS 410
Score = 29.5 bits (58), Expect(5) = 4e-035
Identities = 10/28 (35%), Positives = 15/28 (53%)
Frame = -2 / +3
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKT 513
A ++ SV YCWG K+ E+ K+
Sbjct: 387 ALKEQGASSVGAASYCWGAKVVAELAKS 470
Score = 25.8 bits (50), Expect(5) = 4e-035
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -2 / +3
Query: 335 KIFQGVEHGFACRYN 291
+IF GV HG+ RYN
Sbjct: 648 QIFPGVSHGWTGRYN 692
>gb|BF260055.2|BF260055 HVSMEf0020P20f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0020P20f, mRNA sequence
Length = 803
Score = 81.7 bits (172), Expect(3) = 9e-035
Identities = 29/60 (48%), Positives = 42/60 (70%)
Frame = -2 / +2
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPK 393
+V GYCWG K+ E+ K +++ A +SHP VT +D+KEVK PI +LGA+ D T+PP+
Sbjct: 488 TVGAAGYCWGAKVVAELAKANEIHAAVMSHPSFVTVEDIKEVKCPIAVLGAEIDKTSPPR 667
Score = 61.1 bits (127), Expect(3) = 9e-035
Identities = 29/59 (49%), Positives = 33/59 (55%)
Frame = -2 / +2
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG+ VFG+EAP LR+IADKVA +GY VVVPDFL GD
Sbjct: 191 VVDSFGGITAYVSGARESKAAVVLISDVFGFEAPNLRKIADKVASSGYLVVVPDFLHGD 367
Score = 42.8 bits (87), Expect(3) = 9e-035
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = -2 / +2
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
WL+ H+P KA E+AKP+ AALK++G S
Sbjct: 407 WLKEHAPGKAFEEAKPVIAALKEQGVS 487
>gb|AV921854.1|AV921854 AV921854 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags13h19 3', mRNA sequence
Length = 628
Score = 147 bits (315), Expect = 1e-034
Identities = 59/129 (45%), Positives = 81/129 (62%)
Frame = -2 / -3
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+
Sbjct: 614 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAE 435
Query: 416 NDTTTPPKEVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMV 237
D +PP+ V F VL + ++ KIF GV HG+ RYN D AVK+AE AL M+
Sbjct: 434 TDVMSPPELVKEFEQVLSSNSGIAHFVKIFPGVSHGWTVRYNGEDAAAVKSAEEALTDMI 255
Query: 236 NWFNKHLN* 210
+WFNK+L *
Sbjct: 254 DWFNKNLK* 228
Score = 111 bits (238), Expect = 5e-024
Identities = 57/121 (47%), Positives = 68/121 (56%)
Frame = +1 / +1
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
+ LR LLNQ M RA SA+ TA S LYL +P TP KIL * L TC+N
Sbjct: 226 VHLRFLLNQSIMSVRASSALFTAAASSPLYLTVHP*ETPGKILTK*AIPELDDRTCSNSL 405
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ*PPTA 567
T+ GG + S AP ++GHFTSF+SS VT GC+ SL F S +F PQQ*P
Sbjct: 406 TNSGGDITSVSAPSTAMGHFTSFISSTVTKEGCDITAVWISLAFANSATTFAPQQ*PAAP 585
Query: 568 T 570
T
Sbjct: 586 T 588
>gb|AV936314.1|AV936314 AV936314 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal10o04 3', mRNA sequence
Length = 614
Score = 147 bits (315), Expect = 1e-034
Identities = 59/129 (45%), Positives = 81/129 (62%)
Frame = -2 / -3
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+
Sbjct: 600 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAE 421
Query: 416 NDTTTPPKEVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMV 237
D +PP+ V F VL + ++ KIF GV HG+ RYN D AVK+AE AL M+
Sbjct: 420 TDVMSPPELVKEFEQVLSSNSGIAHFVKIFPGVSHGWTVRYNGEDAAAVKSAEEALTDMI 241
Query: 236 NWFNKHLN* 210
+WFNK+L *
Sbjct: 240 DWFNKNLK* 214
Score = 111 bits (238), Expect = 5e-024
Identities = 57/121 (47%), Positives = 68/121 (56%)
Frame = +1 / +2
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
+ LR LLNQ M RA SA+ TA S LYL +P TP KIL * L TC+N
Sbjct: 212 VHLRFLLNQSIMSVRASSALFTAAASSPLYLTVHP*ETPGKILTK*AIPELDDRTCSNSL 391
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ*PPTA 567
T+ GG + S AP ++GHFTSF+SS VT GC+ SL F S +F PQQ*P
Sbjct: 392 TNSGGDITSVSAPSTAMGHFTSFISSTVTKEGCDITAVWISLAFANSATTFAPQQ*PAAP 571
Query: 568 T 570
T
Sbjct: 572 T 574
>gb|BQ663284.1|BQ663284 HU01O19w HU Hordeum vulgare subsp. vulgare cDNA clone HU01O19
3-PRIME, mRNA sequence
Length = 627
Score = 147 bits (315), Expect = 1e-034
Identities = 59/129 (45%), Positives = 81/129 (62%)
Frame = -2 / -1
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+
Sbjct: 618 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAE 439
Query: 416 NDTTTPPKEVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMV 237
D +PP+ V F VL + ++ KIF GV HG+ RYN D AVK+AE AL M+
Sbjct: 438 TDVMSPPELVKEFEQVLSSNSGIAHFVKIFPGVSHGWTVRYNGEDAAAVKSAEEALTDMI 259
Query: 236 NWFNKHLN* 210
+WFNK+L *
Sbjct: 258 DWFNKNLK* 232
Score = 111 bits (238), Expect = 5e-024
Identities = 57/121 (47%), Positives = 68/121 (56%)
Frame = +1 / +2
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
+ LR LLNQ M RA SA+ TA S LYL +P TP KIL * L TC+N
Sbjct: 230 VHLRFLLNQSIMSVRASSALFTAAASSPLYLTVHP*ETPGKILTK*AIPELDDRTCSNSL 409
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ*PPTA 567
T+ GG + S AP ++GHFTSF+SS VT GC+ SL F S +F PQQ*P
Sbjct: 410 TNSGGDITSVSAPSTAMGHFTSFISSTVTKEGCDITAVWISLAFANSATTFAPQQ*PAAP 589
Query: 568 T 570
T
Sbjct: 590 T 592
>gb|AV935981.1|AV935981 AV935981 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal7f16 3', mRNA sequence
Length = 572
Score = 146 bits (314), Expect = 2e-034
Identities = 58/121 (47%), Positives = 78/121 (64%)
Frame = -2 / -2
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPK 393
SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+ D +PP+
Sbjct: 550 SVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAETDVMSPPE 371
Query: 392 EVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN 213
V F VL + ++ KIF GV HG+ RYN D AVK+AE AL M++WFNK+L
Sbjct: 370 LVKEFEQVLSSNSGIAHFVKIFPGVSHGWTVRYNGEDAAAVKSAEEALTDMIDWFNKNLK 191
Query: 212 * 210
*
Sbjct: 190 * 188
Score = 111 bits (238), Expect = 5e-024
Identities = 57/121 (47%), Positives = 68/121 (56%)
Frame = +1 / +3
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
+ LR LLNQ M RA SA+ TA S LYL +P TP KIL * L TC+N
Sbjct: 186 VHLRFLLNQSIMSVRASSALFTAAASSPLYLTVHP*ETPGKILTK*AIPELDDRTCSNSL 365
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPPQQ*PPTA 567
T+ GG + S AP ++GHFTSF+SS VT GC+ SL F S +F PQQ*P
Sbjct: 366 TNSGGDITSVSAPSTAMGHFTSFISSTVTKEGCDITAVWISLAFANSATTFAPQQ*PAAP 545
Query: 568 T 570
T
Sbjct: 546 T 548
>gb|BQ464865.1|BQ464865 HU01O19T HU Hordeum vulgare subsp. vulgare cDNA clone HU01O19
5-PRIME, mRNA sequence
Length = 613
Score = 83.5 bits (176), Expect(3) = 1e-033
Identities = 31/70 (44%), Positives = 45/70 (64%)
Frame = -2 / +1
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+
Sbjct: 400 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAE 579
Query: 416 NDTTTPPKEV 387
D +PP+ V
Sbjct: 580 TDVMSPPELV 609
Score = 59.3 bits (123), Expect(3) = 1e-033
Identities = 24/31 (77%), Positives = 27/31 (87%)
Frame = -2 / +1
Query: 773 FGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
FG+ AP LR+IADKVA +GYFVVVPDFL GD
Sbjct: 211 FGFGAPNLRKIADKVAASGYFVVVPDFLHGD 303
Score = 38.6 bits (78), Expect(3) = 1e-033
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = -2 / +1
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++G S
Sbjct: 343 WIKEHTAGKAFEEAKPVIAALKEQGAS 423
>gb|AV915236.1|AV915236 AV915236 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags13h19 5', mRNA sequence
Length = 607
Score = 79.4 bits (167), Expect(3) = 5e-033
Identities = 29/66 (43%), Positives = 42/66 (63%)
Frame = -2 / +3
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+
Sbjct: 408 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAE 587
Query: 416 NDTTTP 399
D +P
Sbjct: 588 TDVMSP 605
Score = 61.6 bits (128), Expect(3) = 5e-033
Identities = 29/59 (49%), Positives = 34/59 (57%)
Frame = -2 / +3
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG++ VFG+ AP LR+IADKVA +GYFVVVPDFL GD
Sbjct: 135 VVDSFGGIKAYVAGAQESKAAVVLISDVFGFGAPNLRKIADKVAASGYFVVVPDFLHGD 311
Score = 38.6 bits (78), Expect(3) = 5e-033
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = -2 / +3
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++G S
Sbjct: 351 WIKEHTAGKAFEEAKPVIAALKEQGAS 431
>gb|AV934062.1|AV934062 AV934062 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baalo04 5', mRNA sequence
Length = 534
Score = 79.4 bits (167), Expect(3) = 5e-033
Identities = 29/66 (43%), Positives = 42/66 (63%)
Frame = -2 / +2
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+
Sbjct: 335 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAE 514
Query: 416 NDTTTP 399
D +P
Sbjct: 515 TDVMSP 532
Score = 61.6 bits (128), Expect(3) = 5e-033
Identities = 29/59 (49%), Positives = 34/59 (57%)
Frame = -2 / +2
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG++ VFG+ AP LR+IADKVA +GYFVVVPDFL GD
Sbjct: 62 VVDSFGGIKAYVAGAQESKAAVVLISDVFGFGAPNLRKIADKVAASGYFVVVPDFLHGD 238
Score = 38.6 bits (78), Expect(3) = 5e-033
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = -2 / +2
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++G S
Sbjct: 278 WIKEHTAGKAFEEAKPVIAALKEQGAS 358
>gb|BF621761.2|BF621761 HVSMEa0012H13f Hordeum vulgare seedling shoot EST library
HVcDNA0001 (Cold stress) Hordeum vulgare subsp. vulgare
cDNA clone HVSMEa0012H13f, mRNA sequence
Length = 908
Score = 73.0 bits (153), Expect(3) = 3e-032
Identities = 27/60 (45%), Positives = 39/60 (65%)
Frame = -2 / +1
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPK 393
+V GYCWG K+ E+ K +++ A +SHP T +D+KEVK I +LGA+ D T PP+
Sbjct: 379 TVGAAGYCWGAKVVAELAKANEIHAAVMSHPSFGTVEDIKEVKCAIAVLGAEIDKTFPPE 558
Score = 61.1 bits (127), Expect(3) = 3e-032
Identities = 29/59 (49%), Positives = 33/59 (55%)
Frame = -2 / +1
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG+ VFG+EAP LR+IADKVA +GY VVVPDFL GD
Sbjct: 82 VVDSFGGITAYVSGARESKAAVVLISDVFGFEAPNLRKIADKVASSGYLVVVPDFLHGD 258
Score = 42.8 bits (87), Expect(3) = 3e-032
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = -2 / +1
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
WL+ H+P KA E+AKP+ AALK++G S
Sbjct: 298 WLKEHAPGKAFEEAKPVIAALKEQGVS 378
>gb|BJ475625.1|BJ475625 BJ475625 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal26n14 3', mRNA sequence
Length = 545
Score = 127 bits (273), Expect = 7e-029
Identities = 52/112 (46%), Positives = 72/112 (64%)
Frame = -2 / -1
Query: 545 GGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPKEVYRFVHVL 366
G K+ E+ K ++++ +SHP VT DD+KEVK PI +LGA+ D +PP+ V F VL
Sbjct: 515 GAKVVAELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAETDVMSPPELVKEFEQVL 336
Query: 365 RERHEVPYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN* 210
+ ++ KIF GV HG+ RYN D AVK+AE AL M++WFNK+L *
Sbjct: 335 SSNSGIAHFVKIFPGVSHGWTVRYNGEDAAAVKSAEEALTDMIDWFNKNLK* 180
Score = 102 bits (217), Expect = 4e-021
Identities = 52/113 (46%), Positives = 63/113 (55%)
Frame = +1 / +1
Query: 208 IQLRCLLNQLTM*ARAVSAVLTAKGSVVLYLQANPCSTPWKILA**GTSCLSRSTCTNRY 387
+ LR LLNQ M RA SA+ TA S LYL +P TP KIL * L TC+N
Sbjct: 178 VHLRFLLNQSIMSVRASSALFTAAASSPLYLTVHP*ETPGKILTK*AIPELDDRTCSNSL 357
Query: 388 TSFGGVVVSFWAPRISIGHFTSFMSSAVTLYGCERHTALTSLVFPTSTLSFPP 546
T+ GG + S AP ++GHFTSF+SS VT GC+ SL F S +F P
Sbjct: 358 TNSGGDITSVSAPSTAMGHFTSFISSTVTKEGCDITAVWISLAFANSATTFAP 516
>gb|BF258180.2|BF258180 HVSMEf0015A11f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0015A11f, mRNA sequence
Length = 875
Score = 62.9 bits (131), Expect(3) = 2e-028
Identities = 26/56 (46%), Positives = 34/56 (60%)
Frame = -2 / +3
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTT 405
+V GYCWG K+ E+ K ++ A +SHP T +D+KEVK PI LGA D T
Sbjct: 450 TVGAAGYCWGAKVVAELAKAHEIHAGGMSHPSLGTGEDIKEVKCPIAELGAGIDET 617
Score = 58.3 bits (121), Expect(3) = 2e-028
Identities = 28/59 (47%), Positives = 32/59 (54%)
Frame = -2 / +3
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG+ VFG+EAP LR+IADKVA +GY VVVPD L GD
Sbjct: 153 VVDSFGGITAYVSGARESKAAVVLISDVFGFEAPNLRKIADKVASSGYLVVVPDILHGD 329
Score = 42.8 bits (87), Expect(3) = 2e-028
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = -2 / +3
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
WL+ H+P KA E+AKP+ AALK++G S
Sbjct: 369 WLKEHAPGKAFEEAKPVIAALKEQGVS 449
>gb|CA017090.1|CA017090 HV14O23u HV Hordeum vulgare subsp. vulgare cDNA clone HV14O23
3-PRIME, mRNA sequence
Length = 498
Score = 124 bits (265), Expect = 9e-028
Identities = 50/107 (46%), Positives = 74/107 (69%)
Frame = -2 / -1
Query: 527 EVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPKEVYRFVHVLRERHEV 348
E+ K +++ A +SHP VT +D+KEVK PI +LGA+ D T+PP+ V +F VL +
Sbjct: 495 ELAKANEIHAAVMSHPSFVTVEDIKEVKCPIAVLGAEIDKTSPPELVKQFEQVLSSNSGI 316
Query: 347 PYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN*I 207
++ KIF GV HG+ RY + D AVK+AE AL++M++WF+K+L *I
Sbjct: 315 AHFVKIFPGVSHGWTVRYKSEDAGAVKSAEEALSHMIDWFSKNLQ*I 175
>gb|CA011285.1|CA011285 HT06A10u HT Hordeum vulgare subsp. vulgare cDNA clone HT06A10
3-PRIME, mRNA sequence
Length = 568
Score = 123 bits (263), Expect = 2e-027
Identities = 50/106 (47%), Positives = 69/106 (65%)
Frame = -2 / -2
Query: 527 EVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPKEVYRFVHVLRERHEV 348
E+ K ++++ +SHP VT DD+KEVK PI +LGA+ D +PP+ V F VL +
Sbjct: 558 ELAKANEIQTAVMSHPSFVTVDDIKEVKCPIAVLGAETDVMSPPELVKEFEQVLSSNSGI 379
Query: 347 PYYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN* 210
++ KIF GV HG+ RYN D AVK+AE AL M++WFNK+L *
Sbjct: 378 AHFVKIFPGVSHGWTVRYNGEDAAAVKSAEEALTDMIDWFNKNLK* 241
>gb|BQ662063.1|BQ662063 HR01G10u HR Hordeum vulgare subsp. vulgare cDNA clone HR01G10
3-PRIME, mRNA sequence
Length = 535
Score = 122 bits (260), Expect = 5e-027
Identities = 49/106 (46%), Positives = 73/106 (68%)
Frame = -2 / -1
Query: 524 VGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPKEVYRFVHVLRERHEVP 345
+ K +++ A +SHP VT +D+KEVK PI +LGA+ D T+PP+ V +F VL +
Sbjct: 535 LAKANEIHAAVMSHPSFVTVEDIKEVKCPIAVLGAEIDKTSPPELVKQFEQVLSSNSGIA 356
Query: 344 YYAKIFQGVEHGFACRYNTTDPFAVKTAETALAYMVNWFNKHLN*I 207
++ KIF GV HG+ RY + D AVK+AE AL++M++WF+K+L *I
Sbjct: 355 HFVKIFPGVSHGWTVRYKSEDAGAVKSAEEALSHMIDWFSKNLQ*I 218
>gb|BG300201.1|BG300201 HVSMEb0010O04f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0010O04f, mRNA sequence
Length = 531
Score = 111 bits (236), Expect(2) = 1e-026
Identities = 45/68 (66%), Positives = 54/68 (79%)
Frame = -2 / +2
Query: 773 FGYEAPLLRQIADKVAKAGYFVVVPDFLKGDYLDDKKNFMEWLEAHSPVKAAEDAKPLFA 594
+G++APLLR+IADKV +AGY+VVVPDF G D N EW AHSPVKAA+DAKP+FA
Sbjct: 263 YGFQAPLLRKIADKVGEAGYYVVVPDFFNGQPYTDGANRTEWFLAHSPVKAAQDAKPIFA 442
Query: 593 ALKKEGKS 570
ALKKE +S
Sbjct: 443 ALKKEKRS 466
Score = 29.5 bits (58), Expect(2) = 1e-026
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = -2 / +2
Query: 596 AALKKEGKSVAVGGYCWGGKL 534
A K++ V VGGYCWG ++
Sbjct: 443 ALKKEKRSYVGVGGYCWGREI 505
>gb|BM375339.2|BM375339 EBem06_SQ003_A21_R embryo, 21 DPA, no treatment, cv Optic, EBem06
Hordeum vulgare subsp. vulgare cDNA clone
EBem06_SQ003_A21 5', mRNA sequence
Length = 546
Score = 61.6 bits (128), Expect(3) = 2e-026
Identities = 29/59 (49%), Positives = 34/59 (57%)
Frame = -2 / +1
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG++ VFG+ AP LR+IADKVA +GYFVVVPDFL GD
Sbjct: 127 VVDSFGGIKAYVAGAQESKAAVVLISDVFGFGAPNLRKIADKVAASGYFVVVPDFLHGD 303
Score = 57.0 bits (118), Expect(3) = 2e-026
Identities = 20/49 (40%), Positives = 30/49 (61%)
Frame = -2 / +1
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKE 450
A ++ SV GYCWG K+ E+ K ++++ +SHP VT DD+KE
Sbjct: 400 ALKEQGASSVGAAGYCWGAKVVAELAKANEIQTAVMSHPSFVTVDDIKE 546
Score = 38.6 bits (78), Expect(3) = 2e-026
Identities = 14/27 (51%), Positives = 21/27 (77%)
Frame = -2 / +1
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++G S
Sbjct: 343 WIKEHTAGKAFEEAKPVIAALKEQGAS 423
>gb|CK122919.1|CK122919 BES1824101e05 BES1824 Hordeum vulgare subsp. vulgare cDNA clone
MPMGp2010E051 5-PRIME, mRNA sequence
Length = 531
Score = 115 bits (246), Expect = 4e-025
Identities = 47/95 (49%), Positives = 67/95 (70%)
Frame = -2 / +1
Query: 491 LSHPYSVTADDMKEVKWPIEILGAQNDTTTPPKEVYRFVHVLRERHEVPYYAKIFQGVEH 312
+SHP VT +D+KEVK PI +LGA+ D T+PP+ V +F VL + ++ KIF GV H
Sbjct: 4 MSHPSFVTVEDIKEVKCPIAVLGAEIDKTSPPELVKQFEQVLSSNSGIAHFVKIFPGVSH 183
Query: 311 GFACRYNTTDPFAVKTAETALAYMVNWFNKHLN*I 207
G+ RY + D AVK+AE AL++M++WF+K+L *I
Sbjct: 184 GWTVRYKSEDAGAVKSAEEALSHMIDWFSKNLQ*I 288
>gb|BE214395.2|BE214395 HV_CEb0003F06f Hordeum vulgare seedling green leaf EST library
HVcDNA0005 (Blumeria challenged) Hordeum vulgare subsp.
vulgare cDNA clone HV_CEb0003F06f, mRNA sequence
Length = 575
Score = 58.3 bits (121), Expect(3) = 3e-024
Identities = 24/62 (38%), Positives = 35/62 (56%)
Frame = -2 / +3
Query: 596 AALKKEGKSVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQ 417
A ++ SV Y WG K+ E+ K ++++ +SHP VT DD+KE K P +LGA
Sbjct: 387 ALKEQRASSVGAARYLWGAKVISELAKANEIQTAGMSHPSFVTGDDIKEGKCPNRVLGA* 566
Query: 416 ND 411
D
Sbjct: 567 TD 572
Score = 56.5 bits (117), Expect(3) = 3e-024
Identities = 23/31 (74%), Positives = 26/31 (83%)
Frame = -2 / +3
Query: 773 FGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
FG+ AP LR+IADKVA +GYFVV PDFL GD
Sbjct: 198 FGFGAPNLRKIADKVAASGYFVVEPDFLHGD 290
Score = 35.0 bits (70), Expect(3) = 3e-024
Identities = 13/27 (48%), Positives = 20/27 (74%)
Frame = -2 / +3
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
W++ H+ KA E+AKP+ AALK++ S
Sbjct: 330 WIKEHTAGKAFEEAKPVIAALKEQRAS 410
>gb|AV932136.1|AV932136 AV932136 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal2g20 5', mRNA sequence
Length = 622
Score = 61.1 bits (127), Expect(3) = 2e-023
Identities = 29/59 (49%), Positives = 33/59 (55%)
Frame = -2 / +2
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG+ VFG+EAP LR+IADKVA +GY VVVPDFL GD
Sbjct: 227 VVDSFGGITAYVSGARESKAAVVLISDVFGFEAPNLRKIADKVASSGYLVVVPDFLHGD 403
Score = 43.2 bits (88), Expect(3) = 2e-023
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = -2 / +2
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHP 480
+V GYCWG K+ E+ K +++ A +SHP
Sbjct: 524 TVGAAGYCWGAKVVAELAKANEIHAAVMSHP 616
Score = 42.8 bits (87), Expect(3) = 2e-023
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = -2 / +2
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
WL+ H+P KA E+AKP+ AALK++G S
Sbjct: 443 WLKEHAPGKAFEEAKPVIAALKEQGVS 523
>gb|BJ472055.1|BJ472055 BJ472055 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal31o24 5', mRNA sequence
Length = 596
Score = 61.1 bits (127), Expect(3) = 2e-023
Identities = 29/59 (49%), Positives = 33/59 (55%)
Frame = -2 / +3
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG+ VFG+EAP LR+IADKVA +GY VVVPDFL GD
Sbjct: 201 VVDSFGGITAYVSGARESKAAVVLISDVFGFEAPNLRKIADKVASSGYLVVVPDFLHGD 377
Score = 43.2 bits (88), Expect(3) = 2e-023
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = -2 / +3
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHP 480
+V GYCWG K+ E+ K +++ A +SHP
Sbjct: 498 TVGAAGYCWGAKVVAELAKANEIHAAVMSHP 590
Score = 42.8 bits (87), Expect(3) = 2e-023
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = -2 / +3
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
WL+ H+P KA E+AKP+ AALK++G S
Sbjct: 417 WLKEHAPGKAFEEAKPVIAALKEQGVS 497
>gb|AV932651.1|AV932651 AV932651 K. Sato unpublished cDNA library, cv. Haruna Nijo adult,
heading stage top three leaves Hordeum vulgare subsp.
vulgare cDNA clone baal1i11 5', mRNA sequence
Length = 589
Score = 61.1 bits (127), Expect(3) = 2e-023
Identities = 29/59 (49%), Positives = 33/59 (55%)
Frame = -2 / +2
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG+ VFG+EAP LR+IADKVA +GY VVVPDFL GD
Sbjct: 194 VVDSFGGITAYVSGARESKAAVVLISDVFGFEAPNLRKIADKVASSGYLVVVPDFLHGD 370
Score = 43.2 bits (88), Expect(3) = 2e-023
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = -2 / +2
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHP 480
+V GYCWG K+ E+ K +++ A +SHP
Sbjct: 491 TVGAAGYCWGAKVVAELAKANEIHAAVMSHP 583
Score = 42.8 bits (87), Expect(3) = 2e-023
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = -2 / +2
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
WL+ H+P KA E+AKP+ AALK++G S
Sbjct: 410 WLKEHAPGKAFEEAKPVIAALKEQGVS 490
>gb|BQ467934.1|BQ467934 HR01G10r HR Hordeum vulgare subsp. vulgare cDNA clone HR01G10
5-PRIME, mRNA sequence
Length = 549
Score = 61.1 bits (127), Expect(3) = 2e-023
Identities = 29/59 (49%), Positives = 33/59 (55%)
Frame = -2 / +2
Query: 857 VHDLPGGLRXXXXXXXXXXXXXXXXXXVFGYEAPLLRQIADKVAKAGYFVVVPDFLKGD 681
V D GG+ VFG+EAP LR+IADKVA +GY VVVPDFL GD
Sbjct: 149 VVDSFGGITAYVSGARESKAAVVLISDVFGFEAPNLRKIADKVASSGYLVVVPDFLHGD 325
Score = 43.2 bits (88), Expect(3) = 2e-023
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = -2 / +2
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHP 480
+V GYCWG K+ E+ K +++ A +SHP
Sbjct: 446 TVGAAGYCWGAKVVAELAKANEIHAAVMSHP 538
Score = 42.8 bits (87), Expect(3) = 2e-023
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = -2 / +2
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
WL+ H+P KA E+AKP+ AALK++G S
Sbjct: 365 WLKEHAPGKAFEEAKPVIAALKEQGVS 445
>gb|AJ432736.1|AJ432736 AJ432736 S00002 Hordeum vulgare subsp. vulgare cDNA clone
S0000200022E11F1, mRNA sequence
Length = 301
Score = 107 bits (228), Expect = 1e-022
Identities = 41/89 (46%), Positives = 59/89 (66%)
Frame = -2 / +1
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHPYSVTADDMKEVKWPIEILGAQNDTTTPPK 393
+V GYCWG K+ E+ K +++ A +SHP VT +D+KEVK PI +LGA+ D T+PP+
Sbjct: 28 TVGAAGYCWGAKVVAELAKANEIHAAVMSHPSFVTVEDIKEVKCPIAVLGAEIDKTSPPQ 207
Query: 392 EVYRFVHVLRERHEVPYYAKIFQGVEHGF 306
V +F VL + ++AKIF GV HG+
Sbjct: 208 LVKQFEQVLSSNSGIAHFAKIFPGVAHGW 294
>gb|AV912065.1|AV912065 AV912065 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak2j18 3', mRNA sequence
Length = 470
Score = 103 bits (220), Expect = 2e-021
Identities = 43/85 (50%), Positives = 56/85 (65%)
Frame = -2 / -3
Query: 464 DDMKEVKWPIEILGAQNDTTTPPKEVYRFVHVLRERHEVPYYAKIFQGVEHGFACRYNTT 285
DD+KEVK PI +LGA+ D +PP+ V F VL + ++ KIF GV HG+ RYN
Sbjct: 468 DDIKEVKCPIAVLGAETDVMSPPELVKEFEQVLSSNSGIAHFVKIFPGVSHGWTVRYNGE 289
Query: 284 DPFAVKTAETALAYMVNWFNKHLN* 210
D AVK+AE AL M++WFNK+L *
Sbjct: 288 DAAAVKSAEEALTDMIDWFNKNLK* 214
>gb|CV059100.1|CV059100 BNEL44d1 Barley EST endosperm library Hordeum vulgare subsp.
vulgare cDNA clone BNEL44d1 5' similar to Unknown
protein, mRNA sequence
Length = 607
Score = 43.2 bits (88), Expect(4) = 8e-021
Identities = 13/31 (41%), Positives = 20/31 (64%)
Frame = -2 / +3
Query: 572 SVAVGGYCWGGKLSVEVGKTSDVKAVCLSHP 480
+V GYCWG K+ E+ K +++ A +SHP
Sbjct: 447 TVGAAGYCWGAKVVAELAKANEIHAAVMSHP 539
Score = 42.8 bits (87), Expect(4) = 8e-021
Identities = 16/27 (59%), Positives = 22/27 (81%)
Frame = -2 / +3
Query: 650 WLEAHSPVKAAEDAKPLFAALKKEGKS 570
WL+ H+P KA E+AKP+ AALK++G S
Sbjct: 366 WLKEHAPGKAFEEAKPVIAALKEQGVS 446
Score = 39.6 bits (80), Expect(4) = 8e-021
Identities = 17/28 (60%), Positives = 20/28 (71%)
Frame = -2 / +2
Query: 773 FGYEAPLLRQIADKVAKAGYFVVVPDFL 690
FG+EAP LR+IADKVA +GY FL
Sbjct: 233 FGFEAPNLRKIADKVASSGYLSCGARFL 316
Score = 29.5 bits (58), Expect(4) = 8e-021
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = -2 / +1
Query: 461 DMKEVKWPIEILGAQND 411
D+KEVK PI +LGA+ D
Sbjct: 556 DIKEVKCPIAVLGAEXD 606
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 422,966,538
Number of Sequences: 312970
Number of extensions: 6238457
Number of successful extensions: 75625
Number of sequences better than 1.0e-020: 58
length of database: 58,378,179
effective HSP length: 52
effective length of database: 42,103,739
effective search space used: 12546914222
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)