TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2192420.2.1
(738 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|BJ466853.1|BJ466853 BJ466853 K. Sato unpublished cDNA li... 322 1e-087
>gb|BJ466853.1|BJ466853 BJ466853 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags34i14 3', mRNA sequence
Length = 687
Score = 322 bits (698), Expect = 1e-087
Identities = 135/146 (92%), Positives = 143/146 (97%)
Frame = +1 / -3
Query: 10 KIVMVLAATNFPWDIDEALRRRLEKRIYIPLPDFESRKALININLRTVQIAADVNIDEVA 189
KIVMVLAATNFPWDIDEALRRRLEKRIYIPLP FESRK+LI+INLRTV++A DVNIDEVA
Sbjct: 634 KIVMVLAATNFPWDIDEALRRRLEKRIYIPLPSFESRKSLISINLRTVEVATDVNIDEVA 455
Query: 190 RRTEGYSGDDLTNVCRDASMNGMRRKIAGKTRDEIKNMSKDEIAKDPVAMCDFVEALVKV 369
RRTEGYSGDDLTNVCRDASMNGMRRKIAGKTRDEIKNMSKD+I+KDPVAMCDF EALVKV
Sbjct: 454 RRTEGYSGDDLTNVCRDASMNGMRRKIAGKTRDEIKNMSKDDISKDPVAMCDFEEALVKV 275
Query: 370 QKSVSPSDIEKHEKWMAEFGSA*DLG 447
QKSVSPSDIE+HEKWMAEFGSA*+LG
Sbjct: 274 QKSVSPSDIERHEKWMAEFGSA*NLG 197
Score = 219 bits (473), Expect = 1e-056
Identities = 92/145 (63%), Positives = 106/145 (73%)
Frame = -1 / +2
Query: 444 EVSGRPKLSHPLLMLLYV*RRDTLLNLHQSLHKVAHGHRVLGYLVL*HVLDLVAGLACYL 265
+VS R +L HPLL+ V TLL+L QS KVAHGHRVL ++L*HVLDLV GLAC L
Sbjct: 200 KVSSRSELGHPLLVAFDVRG*HTLLDLDQSFFKVAHGHRVL*DVIL*HVLDLVTGLACDL 379
Query: 264 APHAIH*GIAANVCQIISTIAFCPPSNLVDVNICGYLNCSKINVDKCLSTFKIWKWNINT 85
+PH IH I AN+CQ+I TI FCP N VDVNI YLN SKI D+ LS FK WKWNI+T
Sbjct: 380 SPHTIHRSITANICQVIPTIPFCPSCNFVDVNISSYLNRSKIYADE*LSAFKTWKWNIDT 559
Query: 84 LLQPSPQCLINIPWKICSSQNHNNF 10
L QP P+C INIPW++ S QNHNNF
Sbjct: 560 LFQPPPECFINIPWEVGSCQNHNNF 634
Score = 199 bits (429), Expect = 1e-050
Identities = 88/145 (60%), Positives = 104/145 (71%)
Frame = +3 / -2
Query: 9 KNCYGSGCYKFSMGY**GTEAKAGEAYLYSTSRF*K*KGTYQH*S*NSSDSRRC*HRRGC 188
KNCYGSG Y+ MGY** T+A+AG+AYLYSTS+F*K K T+QH S*N S C*HRR C
Sbjct: 635 KNCYGSGSYQLPMGY**STQAEAGKAYLYSTSKF*KQKVTHQHKS*NG*GSY*C*HRRSC 456
Query: 189 SEDRRL*WR*SDKRLPRCLNEWHEAQDSRQDPRRDQEHVKGRDSQGPGGHVRLCXXXXXX 368
++DRR+*W * DK LP C +EW+EA+D RQDP RDQEHVKG +GPGGHVRL
Sbjct: 455 TKDRRV*WG*PDKCLP*CFDEWYEAKDRRQDP*RDQEHVKG*HLKGPGGHVRL*RSFGQG 276
Query: 369 XXXCLAFRHREA*EVDG*VWVCLRP 443
C+ HR EVDG V +CL+P
Sbjct: 275 PEECVTL*HRTPREVDGRVRICLKP 201
Score = 198 bits (426), Expect(2) = 4e-053
Identities = 92/113 (81%), Positives = 98/113 (86%)
Frame = -2 / +3
Query: 452 RWPRSQADPNSAIHFSCFSMSEGETLF*TFTRASTKSHMATGSLAISSFDMFLISSRVLP 273
R PR QADPNSAIHFS SMSEG+TLF T T+AS+KSHMATGS +SSFDMFLISSRVLP
Sbjct: 192 REPRFQADPNSAIHFSWRSMSEGDTLFWTLTKASSKSHMATGSFEMSSFDMFLISSRVLP 371
Query: 272 AILRLMPFIEASRQTFVRSSPL*PSVLRATSSMLTSAAI*TVLRLMLISAFLL 114
AI RL+PFIEASRQTFVRSSPL PSVLRATSSMLTS A TVLR ML+S FLL
Sbjct: 372 AIFRLIPFIEASRQTFVRSSPLYPSVLRATSSMLTSVATSTVLRFMLMSDFLL 530
Score = 166 bits (358), Expect = 9e-041
Identities = 80/146 (54%), Positives = 90/146 (61%)
Frame = +2 / -1
Query: 5 AKKLLWFWLLQIFHGILMRH*GEGWRSVFIFHFQILKVERHLSTLILEQFR*PQMLTSTR 184
++KLLWFW L HGILM+H G GW+SV IFHFQ+LK E H S ILE+ R* MLTST+
Sbjct: 639 SQKLLWFWQLPTSHGILMKHSGGGWKSVSIFHFQVLKAESHSSA*ILERLR*LLMLTSTK 460
Query: 185 LLGGQKAIVEMI*QTFAAMPQ*MA*GAR*QARPAXXXXXXXXXXXXXXXWPCATLWRLW* 364
L GQK IV M *Q FA M +*M *G R QARP WPCATL +LW
Sbjct: 459 LHEGQKGIVGMT*QMFAVMLR*MV*GERSQARPVTRSRTCQRMTSQRTRWPCATLKKLWS 280
Query: 365 RFRRVSRLQT*RSMRSGWLSLGLPET 442
R RRV T + RSGW S L ET
Sbjct: 279 RSRRVCHPLTSNATRSGWPSSDLLET 202
Score = 87.2 bits (184), Expect(2) = 1e-025
Identities = 44/78 (56%), Positives = 48/78 (61%)
Frame = -3 / +1
Query: 244 RHRGKRXXXXXXXXXXXSEQPRRC*HLRLSELF*D*C**VPFYFQNLEVEYKYASPAFAS 65
+H GK Q RRC*H L + F*D C**V F FQNLEVEY+YA PA A
Sbjct: 400 KHHGKHLSGHPHYTLLSFVQLRRC*HQ*LPQPF*DLC**VTFCFQNLEVEYRYAFPASA* 579
Query: 64 VPHQYPMENL*QPEP*QF 11
V HQYPM + * PEP*QF
Sbjct: 580 VLHQYPMGSW*LPEP*QF 633
Score = 49.2 bits (101), Expect(2) = 1e-025
Identities = 22/67 (32%), Positives = 32/67 (47%)
Frame = -3 / +1
Query: 502 GMPCQPTQNECLNRTQADGRGLRQTQTQPSTSHASLCLKARHXXXXXXXXXXSRTWPPGP 323
G+ + T + + + +G +Q +T+PSTS C + H SRTWPPGP
Sbjct: 142 GIKHKQTSMDKIQQALNGNQGFKQIRTRPSTSRGVRCQRVTHSSGP*PKLLQSRTWPPGP 321
Query: 322 WLSRPLT 302
PLT
Sbjct: 322 LRCHPLT 342
Score = 30.4 bits (60), Expect(2) = 4e-053
Identities = 12/13 (92%), Positives = 12/13 (92%)
Frame = -2 / +3
Query: 47 HGKFVAARTITIF 9
HGK VAARTITIF
Sbjct: 597 HGKLVAARTITIF 635
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 344,276,688
Number of Sequences: 312970
Number of extensions: 5226559
Number of successful extensions: 54224
Number of sequences better than 1.0e-020: 1
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8228841546
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)