TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 1804898.2.1
(739 letters)
Database: Hordeum_nucl_with_EST.fasta
312,970 sequences; 175,134,539 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AV836790.1|AV836790 AV836790 K. Sato unpublished cDNA li... 78 8e-030
gb|BM817329.1|BM817329 HC105E06_T3.ab1 HC Hordeum vulgare s... 79 4e-027
gb|BF628874.2|BF628874 HVSMEb0009A12f Hordeum vulgare seedl... 70 4e-025
gb|BJ449787.1|BJ449787 BJ449787 K. Sato unpublished cDNA li... 73 9e-024
emb|X77467.1|HVOMET H.vulgare L. mRNA for flavonoid 7-O-met... 65 1e-022
gb|BJ457207.1|BJ457207 BJ457207 K. Sato unpublished cDNA li... 73 2e-022
gb|BF259471.1|BF259471 HVSMEf0019D06f Hordeum vulgare seedl... 70 3e-022
gb|AV917258.1|AV917258 AV917258 K. Sato unpublished cDNA li... 58 2e-021
gb|AV922427.1|AV922427 AV922427 K. Sato unpublished cDNA li... 58 2e-021
gb|AL503735.1|AL503735 AL503735 Hordeum vulgare Barke roots... 56 3e-021
gb|AV918157.1|AV918157 AV918157 K. Sato unpublished cDNA li... 56 3e-021
gb|BJ466406.1|BJ466406 BJ466406 K. Sato unpublished cDNA li... 56 3e-021
gb|AV930320.1|AV930320 AV930320 K. Sato unpublished cDNA li... 54 5e-021
gb|AV929534.1|AV929534 AV929534 K. Sato unpublished cDNA li... 56 6e-021
>gb|AV836790.1|AV836790 AV836790 K. Sato unpublished cDNA library: Hordeum vulgare subsp.
vulgare seedling leaves second leaf stage Hordeum
vulgare subsp. vulgare cDNA clone basd3c22, mRNA
sequence
Length = 696
Score = 77.6 bits (163), Expect(3) = 2e-026
Identities = 35/59 (59%), Positives = 43/59 (72%)
Frame = -2 / +1
Query: 297 DPSTVSIIITFPPASLPPIASLQRSIILMHLSSSQSCRIHLSMSASADGMCSNMSPPWK 121
+P+T+S+IITFPPASL +A L ILM SS QSC++HLS + SA GM SNMSP K
Sbjct: 268 EPTTISMIITFPPASLTGMAFLH*RSILMQSSSGQSCKMHLSKTVSAGGMDSNMSPGTK 444
Score = 72.5 bits (152), Expect(3) = 8e-030
Identities = 30/53 (56%), Positives = 38/53 (71%)
Frame = +2 / -2
Query: 332 RETYVLDLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP* 490
+E VL + +++G +REEHEWR+IF AGF DYKIT GI SIIEV+P*
Sbjct: 239 KEAEVLFDVFMMYIDGIQREEHEWRKIFFEAGFSDYKITPVTGIRSIIEVYP* 81
Score = 63.4 bits (132), Expect(3) = 8e-030
Identities = 26/59 (44%), Positives = 33/59 (55%)
Frame = +2 / -2
Query: 122 FHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERCXXXXXXXXXXXXXXXXDTVLGS 298
F GDMFE IP AD ++LK ILHDW ++ CIKI+ +C D V+GS
Sbjct: 443 FVPGDMFESIPPADTVLLKCILHDWPDEDCIKILRQCKKAIPVRDAGGKVIIMDMVVGS 267
Score = 48.7 bits (100), Expect(3) = 2e-026
Identities = 22/37 (59%), Positives = 28/37 (75%)
Frame = -2 / +1
Query: 486 GKTSMMDGMPRVCVIL*SRNPAARKILLHSCSSRSAP 376
G TS+++ +P VIL*S NPA++KI LHSCSSR P
Sbjct: 85 G*TSIIERIPVTGVIL*SLNPASKKIFLHSCSSRWIP 195
Score = 32.2 bits (64), Expect(3) = 8e-030
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +2 / -2
Query: 56 RCTVMDLPHVVAEAPA 103
+CTV+DLP VVA APA
Sbjct: 506 KCTVLDLPRVVAGAPA 459
Score = 29.9 bits (59), Expect(3) = 2e-026
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = -2 / +1
Query: 102 AGASATTWGRSMTVHL 55
AGA ATT GRS TVHL
Sbjct: 460 AGAPATTRGRSSTVHL 507
>gb|BM817329.1|BM817329 HC105E06_T3.ab1 HC Hordeum vulgare subsp. vulgare cDNA clone
HC105E06_T3.ab1 similar to Hexaprenyldihydroxybenzoate
methyltransferase,(S)-scoulerine 9-O-methyltransferase,
mRNA sequence
Length = 885
Score = 79.0 bits (166), Expect(3) = 5e-024
Identities = 36/59 (61%), Positives = 42/59 (71%)
Frame = -2 / -3
Query: 297 DPSTVSIIITFPPASLPPIASLQRSIILMHLSSSQSCRIHLSMSASADGMCSNMSPPWK 121
DP+T+S+IITFPPASL +A L LM SS QSC+IHLS + SA GM SNMSP K
Sbjct: 601 DPTTISMIITFPPASLAGMAFLH*RSTLMQSSSGQSCKIHLSRTVSAGGMYSNMSPGTK 425
Score = 72.5 bits (152), Expect(3) = 4e-027
Identities = 27/59 (45%), Positives = 35/59 (59%)
Frame = +2 / +3
Query: 122 FHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERCXXXXXXXXXXXXXXXXDTVLGS 298
F GDMFE+IP AD ++LKWILHDW +D CIK++ +C D V+GS
Sbjct: 426 FVPGDMFEYIPPADTVLLKWILHDWPDDDCIKVLRQCKKAIPARDAGGKVIIMDMVVGS 602
Score = 54.2 bits (112), Expect(3) = 4e-027
Identities = 18/28 (64%), Positives = 24/28 (85%)
Frame = +2 / +3
Query: 362 LSFVNGAEREEHEWRRIFLAAGFRDYKI 445
+ +++G +REEHEWR+IFL AGF DYKI
Sbjct: 660 MMYIDGIQREEHEWRKIFLXAGFSDYKI 743
Score = 39.6 bits (80), Expect(3) = 5e-024
Identities = 17/23 (73%), Positives = 18/23 (78%)
Frame = -2 / -3
Query: 444 IL*SRNPAARKILLHSCSSRSAP 376
IL*S NPA +KI LHSCSSR P
Sbjct: 742 IL*SLNPAXKKIFLHSCSSRWIP 674
Score = 32.2 bits (64), Expect(3) = 4e-027
Identities = 12/16 (75%), Positives = 14/16 (87%)
Frame = +2 / +3
Query: 56 RCTVMDLPHVVAEAPA 103
+CTV+DLP VVA APA
Sbjct: 363 KCTVLDLPRVVAGAPA 410
Score = 29.9 bits (59), Expect(3) = 5e-024
Identities = 13/16 (81%), Positives = 13/16 (81%)
Frame = -2 / -3
Query: 102 AGASATTWGRSMTVHL 55
AGA ATT GRS TVHL
Sbjct: 409 AGAPATTRGRSSTVHL 362
>gb|BF628874.2|BF628874 HVSMEb0009A12f Hordeum vulgare seedling shoot EST library
HVcDNA0002 (Dehydration stress) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEb0009A12f, mRNA sequence
Length = 875
Score = 70.3 bits (147), Expect(2) = 4e-023
Identities = 34/55 (61%), Positives = 37/55 (67%)
Frame = -2 / -3
Query: 294 PSTVSIIITFPPASLPPIASLQRSIILMHLSSSQSCRIHLSMSASADGMCSNMSP 130
P+T+S IITFPPAS IA LQ ILM SS QSC IH + SA GM SNMSP
Sbjct: 210 PTTMSTIITFPPASFGAIALLQFFSILMQSSSLQSCIIHFRKTTSACGMLSNMSP 46
Score = 68.9 bits (144), Expect(2) = 4e-025
Identities = 25/60 (41%), Positives = 33/60 (55%)
Frame = +2 / +2
Query: 116 LSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERCXXXXXXXXXXXXXXXXDTVLG 295
+ + GDMFE IP AD + LKWI+HDW +D CIKI++ C D V+G
Sbjct: 32 VQYIAGDMFESIPQADVVFLKWIMHDWSDDDCIKILKNCNKAIAPKDAGGKVIIVDMVVG 211
Score = 65.7 bits (137), Expect(2) = 4e-025
Identities = 27/53 (50%), Positives = 38/53 (71%)
Frame = +2 / +2
Query: 332 RETYVLDLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP* 490
+ET VL + +NG ER+E EW++I +AAGF D+KIT G+ SIIE++P*
Sbjct: 236 KETQVLFDLYIMLLNGIERDEQEWKKIIMAAGFSDFKITPILGVRSIIELYP* 394
Score = 57.4 bits (119), Expect(2) = 4e-023
Identities = 28/53 (52%), Positives = 33/53 (62%)
Frame = -2 / -3
Query: 489 HGKTSMMDGMPRVCVIL*SRNPAARKILLHSCSSRSAPFTKLSMWRSNTYVSL 331
HG +SM+D PR+ VIL S NPAA I HSCSSRS P + + T VSL
Sbjct: 393 HG*SSMIDRTPRIGVILKSLNPAAMIIFFHSCSSRSMPLRSMM*RSNKTCVSL 235
>gb|BJ449787.1|BJ449787 BJ449787 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak26l07 5', mRNA sequence
Length = 609
Score = 72.5 bits (152), Expect(4) = 9e-024
Identities = 33/72 (45%), Positives = 40/72 (55%)
Frame = +2 / +1
Query: 83 VVAEAPAGTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERCXXXXXXXXXX 262
V +A AG LSF GDMF+ IPSADA++LK ILHDW D C+KI++ C
Sbjct: 106 VSGQAAAGDDILSFVAGDMFQSIPSADAVLLKNILHDWGHDDCVKILKCCKEAIPARNAG 285
Query: 263 XXXXXXDTVLGS 298
D V GS
Sbjct: 286 GKVIIIDMVRGS 321
Score = 36.8 bits (74), Expect(4) = 9e-024
Identities = 13/25 (52%), Positives = 17/25 (68%)
Frame = +2 / +1
Query: 362 LSFVNGAEREEHEWRRIFLAAGFRD 436
+ ++ G ER E EW+RIF AGF D
Sbjct: 373 MMYITGIERNEIEWKRIFSDAGFSD 447
Score = 30.9 bits (61), Expect(4) = 9e-024
Identities = 11/16 (68%), Positives = 12/16 (75%)
Frame = +2 / +1
Query: 56 RCTVMDLPHVVAEAPA 103
+CTVMDLPHVV A
Sbjct: 73 KCTVMDLPHVVVSGQA 120
Score = 24.4 bits (47), Expect(4) = 9e-024
Identities = 10/19 (52%), Positives = 13/19 (68%)
Frame = +2 / +1
Query: 434 DYKITHTRGIPSIIEVFP* 490
DYKI G S+IE++P*
Sbjct: 448 DYKILPVLGPYSVIEIYP* 504
>emb|X77467.1|HVOMET H.vulgare L. mRNA for flavonoid 7-O-methyltransferase
Length = 1402
Score = 64.8 bits (135), Expect(3) = 1e-022
Identities = 23/61 (37%), Positives = 35/61 (57%)
Frame = +2 / +1
Query: 116 LSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERCXXXXXXXXXXXXXXXXDTVLG 295
+ F GDM E +P A+A++LK++LH+W + C+KI+ RC DTV+G
Sbjct: 850 VEFVAGDMMEFVPPAEAVLLKYVLHNWSDQDCVKILTRCREAISHGEKAGKVIIIDTVVG 1029
Query: 296 S 298
S
Sbjct: 1030 S 1032
Score = 57.0 bits (118), Expect(3) = 1e-022
Identities = 24/48 (50%), Positives = 31/48 (64%)
Frame = +2 / +1
Query: 347 LDLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP* 490
+DL ++ NG REE W +IFL AGF YKI + G+ S+IEV P*
Sbjct: 1066 MDLSMMMLFNGKVREEQNWHKIFLEAGFSHYKIHNVLGMRSLIEVQP* 1209
Score = 22.1 bits (42), Expect(3) = 1e-022
Identities = 6/11 (54%), Positives = 10/11 (90%)
Frame = +2 / +1
Query: 56 RCTVMDLPHVV 88
+C+V+DLP V+
Sbjct: 793 KCSVLDLPQVI 825
>gb|BJ457207.1|BJ457207 BJ457207 K. Sato unpublished cDNA library, cv. Akashinriki
vegetative stage leaves Hordeum vulgare subsp. vulgare
cDNA clone baak26l07 3', mRNA sequence
Length = 565
Score = 72.5 bits (152), Expect(4) = 2e-022
Identities = 33/72 (45%), Positives = 40/72 (55%)
Frame = +2 / -3
Query: 83 VVAEAPAGTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERCXXXXXXXXXX 262
V +A AG LSF GDMF+ IPSADA++LK ILHDW D C+KI++ C
Sbjct: 482 VSGQAAAGDDILSFVAGDMFQSIPSADAVLLKNILHDWGHDDCVKILKCCKEAIPARNAG 303
Query: 263 XXXXXXDTVLGS 298
D V GS
Sbjct: 302 GKVIIIDMVRGS 267
Score = 34.1 bits (68), Expect(4) = 2e-022
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = +2 / -3
Query: 362 LSFVNGAEREEHEWRRIFLAAGFRD 436
+ ++ G ER E EW+ IF AGF D
Sbjct: 215 MMYITGIERNEIEWKXIFSDAGFSD 141
Score = 30.9 bits (61), Expect(4) = 2e-022
Identities = 11/16 (68%), Positives = 12/16 (75%)
Frame = +2 / -3
Query: 56 RCTVMDLPHVVAEAPA 103
+CTVMDLPHVV A
Sbjct: 515 KCTVMDLPHVVVSGQA 468
Score = 22.1 bits (42), Expect(4) = 2e-022
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = +2 / -3
Query: 434 DYKITHTRGIPSIIEVFP* 490
DY I G S+IE++P*
Sbjct: 140 DYXIXPVLGPYSVIEIYP* 84
>gb|BF259471.1|BF259471 HVSMEf0019D06f Hordeum vulgare seedling root EST library HVcDNA0007
(Etiolated and unstressed) Hordeum vulgare subsp.
vulgare cDNA clone HVSMEf0019D06f, mRNA sequence
Length = 587
Score = 70.3 bits (147), Expect(3) = 3e-022
Identities = 22/39 (56%), Positives = 32/39 (82%)
Frame = +2 / +1
Query: 116 LSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
+SF GDMF++IP ADAL LKW+ HDW ++ C+KI+++C
Sbjct: 277 VSFISGDMFKYIPPADALFLKWVFHDWGDEDCVKILKKC 393
Score = 37.3 bits (75), Expect(3) = 3e-022
Identities = 13/15 (86%), Positives = 15/15 (100%)
Frame = +2 / +1
Query: 56 RCTVMDLPHVVAEAP 100
+CTV+DLPHVVAEAP
Sbjct: 220 KCTVLDLPHVVAEAP 264
Score = 35.0 bits (70), Expect(3) = 3e-022
Identities = 15/35 (42%), Positives = 21/35 (60%)
Frame = +2 / +1
Query: 332 RETYVLDLHILSFVNGAEREEHEWRRIFLAAGFRD 436
RET V + F+ G EREE E + +F+ AGF +
Sbjct: 481 RETQVXCDLFIMFLGGVEREEFEVKNLFMQAGFTE 585
>gb|AV917258.1|AV917258 AV917258 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags18c14 5', mRNA sequence
Length = 582
Score = 58.3 bits (121), Expect(3) = 2e-021
Identities = 20/49 (40%), Positives = 34/49 (69%)
Frame = +2 / +1
Query: 344 VLDLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP* 490
++D+ ++ G +R E++WR++F+ AGF+DYKI G +IEV+P*
Sbjct: 379 LMDMLMMVTTRGVQRSENDWRKLFMEAGFKDYKIVRKLGARCVIEVYP* 525
Score = 53.3 bits (110), Expect(3) = 2e-021
Identities = 16/39 (41%), Positives = 29/39 (74%)
Frame = +2 / +1
Query: 116 LSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
+++ GD+F +PS+ A+MLK +LH W ++ C+KI+ +C
Sbjct: 163 VNYVAGDLFHTVPSSQAVMLKLVLHFWSDEDCVKILTQC 279
Score = 28.1 bits (55), Expect(3) = 2e-021
Identities = 9/15 (60%), Positives = 13/15 (86%)
Frame = +2 / +1
Query: 56 RCTVMDLPHVVAEAP 100
+CTV+DLP V+ +AP
Sbjct: 106 KCTVLDLPKVIDKAP 150
>gb|AV922427.1|AV922427 AV922427 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags18c14 3', mRNA sequence
Length = 530
Score = 58.3 bits (121), Expect(3) = 2e-021
Identities = 20/49 (40%), Positives = 34/49 (69%)
Frame = +2 / -2
Query: 344 VLDLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP* 490
++D+ ++ G +R E++WR++F+ AGF+DYKI G +IEV+P*
Sbjct: 163 LMDMLMMVTTRGVQRSENDWRKLFMEAGFKDYKIVRKLGARCVIEVYP* 17
Score = 53.3 bits (110), Expect(3) = 2e-021
Identities = 16/39 (41%), Positives = 29/39 (74%)
Frame = +2 / -2
Query: 116 LSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
+++ GD+F +PS+ A+MLK +LH W ++ C+KI+ +C
Sbjct: 379 VNYVAGDLFHTVPSSQAVMLKLVLHFWSDEDCVKILTQC 263
Score = 28.1 bits (55), Expect(3) = 2e-021
Identities = 9/15 (60%), Positives = 13/15 (86%)
Frame = +2 / -2
Query: 56 RCTVMDLPHVVAEAP 100
+CTV+DLP V+ +AP
Sbjct: 436 KCTVLDLPKVIDKAP 392
>gb|AL503735.1|AL503735 AL503735 Hordeum vulgare Barke roots Hordeum vulgare subsp. vulgare
cDNA clone HW03D24T 5', mRNA sequence
Length = 700
Score = 56.1 bits (116), Expect(3) = 3e-021
Identities = 19/49 (38%), Positives = 30/49 (61%)
Frame = +2 / +1
Query: 86 VAEAPAGTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
V E +++ GD+F +P A A+MLK +LH W +D C+KI+ +C
Sbjct: 163 VIEKTPSDCVVNYVAGDLFHTVPKAQAVMLKLVLHHWSDDDCVKILTQC 309
Score = 55.6 bits (115), Expect(3) = 3e-021
Identities = 20/47 (42%), Positives = 31/47 (65%)
Frame = +2 / +1
Query: 344 VLDLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVF 484
++D+ +L F G +R E++WR +F+ AGF DYKI G +IEV+
Sbjct: 409 LMDMLMLVFTRGRQRSENDWRDLFMKAGFTDYKIIKKMGARGVIEVY 549
Score = 27.2 bits (53), Expect(3) = 3e-021
Identities = 8/16 (50%), Positives = 13/16 (81%)
Frame = +2 / +1
Query: 56 RCTVMDLPHVVAEAPA 103
+CTV+DLP V+ + P+
Sbjct: 136 KCTVLDLPKVIEKTPS 183
>gb|AV918157.1|AV918157 AV918157 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags20b03 3', mRNA sequence
Length = 676
Score = 56.1 bits (116), Expect(3) = 3e-021
Identities = 19/49 (38%), Positives = 30/49 (61%)
Frame = +2 / -1
Query: 86 VAEAPAGTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
V E +++ GD+F +P A A+MLK +LH W +D C+KI+ +C
Sbjct: 532 VIEKTPSDCVVNYVAGDLFHTVPKAQAVMLKLVLHHWSDDDCVKILTQC 386
Score = 55.6 bits (115), Expect(3) = 3e-021
Identities = 20/47 (42%), Positives = 31/47 (65%)
Frame = +2 / -1
Query: 344 VLDLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVF 484
++D+ +L F G +R E++WR +F+ AGF DYKI G +IEV+
Sbjct: 286 LMDMLMLVFTRGRQRSENDWRDLFMKAGFTDYKIIKKMGARGVIEVY 146
Score = 27.2 bits (53), Expect(3) = 3e-021
Identities = 8/16 (50%), Positives = 13/16 (81%)
Frame = +2 / -1
Query: 56 RCTVMDLPHVVAEAPA 103
+CTV+DLP V+ + P+
Sbjct: 559 KCTVLDLPKVIEKTPS 512
>gb|BJ466406.1|BJ466406 BJ466406 K. Sato unpublished cDNA library, cv. Haruna Nijo
germination shoots Hordeum vulgare subsp. vulgare cDNA
clone bags31p18 3', mRNA sequence
Length = 613
Score = 56.1 bits (116), Expect(3) = 3e-021
Identities = 19/49 (38%), Positives = 30/49 (61%)
Frame = +2 / -2
Query: 86 VAEAPAGTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
V E +++ GD+F +P A A+MLK +LH W +D C+KI+ +C
Sbjct: 546 VIEKTPSDCVVNYVAGDLFHTVPKAQAVMLKLVLHHWSDDDCVKILTQC 400
Score = 55.6 bits (115), Expect(3) = 3e-021
Identities = 20/47 (42%), Positives = 31/47 (65%)
Frame = +2 / -2
Query: 344 VLDLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVF 484
++D+ +L F G +R E++WR +F+ AGF DYKI G +IEV+
Sbjct: 300 LMDMLMLVFTRGRQRSENDWRDLFMKAGFTDYKIIKKMGARGVIEVY 160
Score = 27.2 bits (53), Expect(3) = 3e-021
Identities = 8/16 (50%), Positives = 13/16 (81%)
Frame = +2 / -2
Query: 56 RCTVMDLPHVVAEAPA 103
+CTV+DLP V+ + P+
Sbjct: 573 KCTVLDLPKVIEKTPS 526
>gb|AV930320.1|AV930320 AV930320 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd22a15 3', mRNA sequence
Length = 664
Score = 54.2 bits (112), Expect(4) = 5e-021
Identities = 22/49 (44%), Positives = 31/49 (63%)
Frame = +2 / -3
Query: 344 VLDLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVFP* 490
++D+ +L G +R+E++W IF AGF DYKI G IIEV+P*
Sbjct: 320 LMDMLMLVCTKGRQRDENDWSTIFTKAGFSDYKIVKKLGARGIIEVYP* 174
Score = 53.3 bits (110), Expect(4) = 5e-021
Identities = 21/61 (34%), Positives = 31/61 (50%)
Frame = +2 / -3
Query: 116 LSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERCXXXXXXXXXXXXXXXXDTVLG 295
+++ GD+F IP A LMLK +LH W ++ CI I+ +C D V+G
Sbjct: 533 VNYVAGDLFHAIPPAQVLMLKLVLHFWSDEDCINILSQCKKAIPSREMGGKVIVIDIVVG 354
Query: 296 S 298
S
Sbjct: 353 S 351
Score = 24.9 bits (48), Expect(4) = 5e-021
Identities = 7/16 (43%), Positives = 12/16 (75%)
Frame = +2 / -3
Query: 56 RCTVMDLPHVVAEAPA 103
+C V+DLP V+ + P+
Sbjct: 590 KCNVLDLPKVIEKVPS 543
Score = 22.6 bits (43), Expect(4) = 5e-021
Identities = 8/18 (44%), Positives = 9/18 (50%)
Frame = +2 / -3
Query: 2 GGRGTXXXXXXXXFPHIQ 55
GG GT FPHI+
Sbjct: 641 GGDGTTARAIVKAFPHIK 588
>gb|AV929534.1|AV929534 AV929534 K. Sato unpublished cDNA library, cv. Haruna Nijo second
leaf stage seedling leaves Hordeum vulgare subsp.
vulgare cDNA clone basd14j15 3', mRNA sequence
Length = 625
Score = 56.1 bits (116), Expect(3) = 6e-021
Identities = 19/49 (38%), Positives = 30/49 (61%)
Frame = +2 / -1
Query: 86 VAEAPAGTAGLSFHGGDMFEHIPSADALMLKWILHDWDEDKCIKIMERC 232
V E +++ GD+F +P A A+MLK +LH W +D C+KI+ +C
Sbjct: 601 VIEKTPSDCVVNYVAGDLFHTVPKAQAVMLKLVLHHWSDDDCVKILTQC 455
Score = 55.6 bits (115), Expect(3) = 6e-021
Identities = 20/47 (42%), Positives = 31/47 (65%)
Frame = +2 / -1
Query: 344 VLDLHILSFVNGAEREEHEWRRIFLAAGFRDYKITHTRGIPSIIEVF 484
++D+ +L F G +R E++WR +F+ AGF DYKI G +IEV+
Sbjct: 355 LMDMLMLVFTRGRQRSENDWRDLFMKAGFTDYKIIKKMGARGVIEVY 215
Score = 26.3 bits (51), Expect(3) = 6e-021
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +2 / -1
Query: 59 CTVMDLPHVVAEAPA 103
CTV+DLP V+ + P+
Sbjct: 625 CTVLDLPKVIEKTPS 581
Database: Hordeum_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:16 PM
Number of letters in database: 175,134,539
Number of sequences in database: 312,970
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 297,071,069
Number of Sequences: 312970
Number of extensions: 4539877
Number of successful extensions: 45671
Number of sequences better than 1.0e-020: 22
length of database: 58,378,179
effective HSP length: 51
effective length of database: 42,416,709
effective search space used: 8228841546
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)