TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCK13d03.yg.3.1
(2611 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816985.1|CN816985 HRO4527_A03_B05ZS5 Lib AA071E1X Aven... 106 2e-046
gb|CN820177.1|CN820177 HRO4412_A07_B14ZS5 Lib AA069E1X Aven... 118 9e-042
gb|BE439135.1|BE439135 CDO465_WHE2G0092F ITEC CDO Oat cDNA ... 102 2e-022
>gb|CN816985.1|CN816985 HRO4527_A03_B05ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4527_A03_B05, mRNA sequence
Length = 608
Score = 106 bits (227), Expect(2) = 2e-046
Identities = 41/74 (55%), Positives = 54/74 (72%)
Frame = +2 / +1
Query: 1997 VVVLISGRPLVVEPYLGDMDALVAAWLPGSEGQGVADVLFGDYGFTGKLPRTWFKSVDQL 2176
V+V+ ++ + + +DALVAAWLPGSEG G+ D +FGD+ F G LP TWF+S DQL
Sbjct: 385 VIVISGRPLVIDQQVMEKIDALVAAWLPGSEGTGITDCIFGDHDFVGTLPVTWFRSSDQL 564
Query: 2177 PMNVGDAHYDPLFP 2218
P+N+GDA YD LFP
Sbjct: 565 PINIGDAKYDLLFP 606
Score = 99.6 bits (211), Expect(2) = 2e-046
Identities = 38/79 (48%), Positives = 53/79 (67%)
Frame = +2 / +1
Query: 1625 KNGKSSYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTAGTTILSGIEA 1804
++GK+ LPL K A +ILVAG+HA+D+G QCGGWTI W G SG T GT+IL I+
Sbjct: 7 EDGKNLKETFLPLAKNAKRILVAGTHADDIGYQCGGWTIAWHGDSGKITLGTSILEAIQE 186
Query: 1805 TVDPSTQVVYSESPDSGVL 1861
+V T+VV+ + P ++
Sbjct: 187 SVGVETEVVHEQCPTDAII 243
Score = 65.2 bits (136), Expect(3) = 3e-024
Identities = 30/56 (53%), Positives = 34/56 (60%)
Frame = -2 / -1
Query: 2217 GKSGS*CASPTFMGSWSTDLNHVRGSFPVNP*SPNSTSATPCPSDPGSHAATSASM 2050
G S ASP F+G+ S DLNHV G+ P SP S P PS PGSHAAT AS+
Sbjct: 605 GNKRSYLASPIFIGN*SEDLNHVTGNVPTKSWSPKMQSVIPVPSLPGSHAATRASI 438
Score = 44.6 bits (91), Expect(3) = 3e-024
Identities = 27/53 (50%), Positives = 30/53 (56%)
Frame = -2 / -1
Query: 2034 STTSGLPEMRTTTHLAAPHTDWITLGPGAGIVRFRLSPNVSAYGGSPTTTIA* 1876
S T GLP+M T+ T I P GI+R LSPN SA G SPTTT A*
Sbjct: 419 SITKGLPDMTITSVGTLLATRVIRSEPLKGILRSVLSPNHSA*GTSPTTTTA* 261
Score = 42.8 bits (87), Expect(3) = 3e-024
Identities = 20/40 (50%), Positives = 22/40 (55%)
Frame = -2 / -1
Query: 1749 CHVIVHPPHWLPRSLAWLPATRILPAFFGRGSNGA*EDLP 1630
C IVHPPH P S A +PAT I AF G N + LP
Sbjct: 131 CQAIVHPPH*YPISSACVPATSIRFAFLANGRNVSFRFLP 12
>gb|CN820177.1|CN820177 HRO4412_A07_B14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4412_A07_B14, mRNA
sequence
Length = 675
Score = 118 bits (252), Expect(2) = 9e-042
Identities = 45/79 (56%), Positives = 57/79 (72%)
Frame = +2 / +1
Query: 1997 VVVLISGRPLVVEPYLGDMDALVAAWLPGSEGQGVADVLFGDYGFTGKLPRTWFKSVDQL 2176
V+V+ ++ L +DALVAAWLPGSEG G+ D +FGD+ F G LP TWF+ DQ
Sbjct: 373 VIVISGRPLVIDPQVLEKIDALVAAWLPGSEGNGITDCIFGDHDFVGTLPVTWFRFSDQF 552
Query: 2177 PMNVGDAHYDPLFPFGFGL 2233
P+N+GDA YDPLFPFG+GL
Sbjct: 553 PINIGDAKYDPLFPFGYGL 609
Score = 73.0 bits (153), Expect(2) = 9e-042
Identities = 28/47 (59%), Positives = 33/47 (70%)
Frame = +2 / +3
Query: 1634 KSSYAPLLPLPKKAGKILVAGSHANDLGNQCGGWTITWQGSSGNTTA 1774
K+ LPL K A +ILVAG+HA+D+G QCGGWTI W G SG TA
Sbjct: 3 KNQKEMFLPLAKNAKRILVAGTHADDIGYQCGGWTIAWHGDSGKITA 143
Score = 72.5 bits (152), Expect(3) = 3e-027
Identities = 32/63 (50%), Positives = 39/63 (61%)
Frame = -2 / -2
Query: 2238 VVSPKPNGKSGS*CASPTFMGSWSTDLNHVRGSFPVNP*SPNSTSATPCPSDPGSHAATS 2059
+ +P P G GS ASP F+G+ S +LNHV G+ P SP S P PS PGSHAAT
Sbjct: 614 IFNP*PKGNKGSYLASPIFIGN*SENLNHVTGNVPTKSWSPKMQSVIPLPSLPGSHAATR 435
Query: 2058 ASM 2050
AS+
Sbjct: 434 ASI 426
Score = 48.7 bits (100), Expect(3) = 3e-027
Identities = 30/65 (46%), Positives = 35/65 (53%)
Frame = -2 / -2
Query: 2070 AATSASMSPRYGSTTSGLPEMRTTTHLAAPHTDWITLGPGAGIVRFRLSPNVSAYGGSPT 1891
A ++ S GS T GLP+M T+ T I P GI+R LSPN SA G SPT
Sbjct: 443 ATRASIFSSTCGSITKGLPDMTITSVGTLLATRVIRSEPLKGILRSVLSPNHSA*GTSPT 264
Query: 1890 TTIA* 1876
TT A*
Sbjct: 263 TTTA* 249
Score = 41.8 bits (85), Expect(3) = 3e-027
Identities = 18/33 (54%), Positives = 19/33 (57%)
Frame = -2 / -3
Query: 1749 CHVIVHPPHWLPRSLAWLPATRILPAFFGRGSN 1651
C IVHPPH P S A +PAT I AF G N
Sbjct: 118 CQAIVHPPH*YPISSACVPATSIRFAFLANGRN 20
>gb|BE439135.1|BE439135 CDO465_WHE2G0092F ITEC CDO Oat cDNA Library Avena sativa cDNA clone
CDO465_WHE2G0092, mRNA sequence
Length = 284
Score = 102 bits (218), Expect = 2e-022
Identities = 39/47 (82%), Positives = 42/47 (89%)
Frame = +2 / -3
Query: 2117 GDYGFTGKLPRTWFKSVDQLPMNVGDAHYDPLFPFGFGLTTKGTK*V 2257
GDYGF+GKL RTW KSVDQLPMNVGD HYDPLFPFGFGLTT+ K*+
Sbjct: 282 GDYGFSGKLARTWLKSVDQLPMNVGDKHYDPLFPFGFGLTTEAKK*M 142
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 29,352,997
Number of Sequences: 8143
Number of extensions: 467618
Number of successful extensions: 4271
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 46
effective length of database: 1,192,909
effective search space used: 981764107
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)