TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH22b04.yg.2.1
(457 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN820901.1|CN820901 HRO4421_C03_E05ZS5 Lib AA069E1X Aven... 193 1e-050
gb|AF033096.1|AF033096 Avena sativa nonphototropic hypocoty... 185 3e-048
gb|AF033097.1|AF033097 Avena sativa nonphototropic hypocoty... 185 3e-048
emb|X79992.1|ASPK11 A.sativa Aspk11 mRNA 68 4e-022
>gb|CN820901.1|CN820901 HRO4421_C03_E05ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4421_C03_E05, mRNA
sequence
Length = 744
Score = 193 bits (417), Expect = 1e-050
Identities = 79/133 (59%), Positives = 99/133 (74%)
Frame = +1 / +3
Query: 13 FAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCPGGDL 192
FAMK MDK+ + +R K+ RA EREI LDHPFLPTLYT F+T CL+ +FCPGG+L
Sbjct: 141 FAMKAMDKSVMLNRNKVHRAIIEREIYSLLDHPFLPTLYTSFQTPTHICLITDFCPGGEL 320
Query: 193 HTLRQRQPGKYFSEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLSDFD 372
+QP K F E++A+FY AEV++ LEYLH LGIIYRDLKPEN+L++EDGH++L+DFD
Sbjct: 321 FAALDKQPLKIFREESARFYAAEVVIGLEYLHCLGIIYRDLKPENILLQEDGHVVLTDFD 500
Query: 373 LSLRCSVSLTVIK 411
LS S VIK
Sbjct: 501 LSFLTSSKPHVIK 539
>gb|AF033096.1|AF033096 Avena sativa nonphototropic hypocotyl 1 (NPH1-1) mRNA, complete cds
Length = 3467
Score = 185 bits (399), Expect = 3e-048
Identities = 76/126 (60%), Positives = 90/126 (71%)
Frame = +1 / +2
Query: 1 TRSYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCP 180
T YFAMK MDK + +R K+ RA EREIL LDHPFLPTLY F+T CL+ ++ P
Sbjct: 2249 TGEYFAMKAMDKNVMLNRNKVHRANAEREILDMLDHPFLPTLYASFQTKTHICLITDYYP 2428
Query: 181 GGDLHTLRQRQPGKYFSEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIML 360
GG+L L RQP K E A +FY AEV++ALEYLH GIIYRDLKPEN+L+ DGHI L
Sbjct: 2429 GGELFLLLDRQPLKVLREDAVRFYAAEVVIALEYLHCQGIIYRDLKPENILLHRDGHISL 2608
Query: 361 SDFDLS 378
+DFDLS
Sbjct: 2609 TDFDLS 2626
>gb|AF033097.1|AF033097 Avena sativa nonphototropic hypocotyl 1 (NPH1-2) mRNA, complete cds
Length = 3422
Score = 185 bits (399), Expect = 3e-048
Identities = 76/126 (60%), Positives = 90/126 (71%)
Frame = +1 / +2
Query: 1 TRSYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCP 180
T YFAMK MDK + +R K+ RA EREIL LDHPFLPTLY F+T CL+ ++ P
Sbjct: 2186 TGEYFAMKAMDKNVMLNRNKVHRANAEREILDMLDHPFLPTLYASFQTKTHICLITDYYP 2365
Query: 181 GGDLHTLRQRQPGKYFSEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIML 360
GG+L L RQP K E A +FY AEV++ALEYLH GIIYRDLKPEN+L+ DGHI L
Sbjct: 2366 GGELFLLLDRQPLKVLREDAVRFYAAEVVIALEYLHCQGIIYRDLKPENILLHRDGHISL 2545
Query: 361 SDFDLS 378
+DFDLS
Sbjct: 2546 TDFDLS 2563
Score = 94.5 bits (200), Expect = 9e-021
Identities = 48/101 (47%), Positives = 61/101 (60%)
Frame = -3 / -3
Query: 404 TVRLTEQRRDRSKSDSMMWPSSRTRTFSGLRSRYIIPSICRYSNARSTSAT*NFAACSEK 225
T L + R+D+SKS M PS ++ FSG +SR IIP C+YS+A +TSA *N A S +
Sbjct: 2589 TCGLQDVRQDKSKSVKDMCPSL*SKIFSGFKSR*IIPWQCKYSSAMTTSAA*NLTASSRR 2410
Query: 224 YFPGCLWRRV*RSPPGQNSITKHENLSVSK*VYRVGRNGWS 102
F GCL R SPPG S+ + + V Y VGRNGWS
Sbjct: 2409 TFRGCLSRSRKSSPPG**SVIRQICVFV*NDAYNVGRNGWS 2287
>emb|X79992.1|ASPK11 A.sativa Aspk11 mRNA
Length = 1987
Score = 68.0 bits (142), Expect(2) = 4e-022
Identities = 26/51 (50%), Positives = 38/51 (74%)
Frame = +1 / +2
Query: 226 FSEQAAKFYVAEVLLALEYLHMLGIIYRDLKPENVLVREDGHIMLSDFDLS 378
F E+ A+ Y AE++ A+ +LH GI++RDLKPEN+L+ GH ML+DF L+
Sbjct: 1073 FREELARIYTAEIVSAVAHLHANGIMHRDLKPENILLDARGHAMLTDFGLA 1225
Score = 50.6 bits (104), Expect(2) = 4e-022
Identities = 24/64 (37%), Positives = 34/64 (53%)
Frame = +1 / +2
Query: 1 TRSYFAMKVMDKTSLASRKKLLRAQTEREILQSLDHPFLPTLYTHFETDKFSCLVMEFCP 180
T +AMKVM K + + + ER+IL +DHPF+ L F+T LV++F
Sbjct: 854 TSEIYAMKVMRKDKILEKNHAEYMKAERDILTKVDHPFVVQLRYSFQTKYRLYLVLDFVN 1033
Query: 181 GGDL 192
GG L
Sbjct: 1034 GGHL 1045
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,002,314
Number of Sequences: 8143
Number of extensions: 91859
Number of successful extensions: 2878
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 41
effective length of database: 1,233,624
effective search space used: 135698640
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)