TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QCH17d04.yg.2.1
(637 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818044.1|CN818044 HRO4472_D08_G16ZS5 Lib AA070E1X Aven... 388 e-109
gb|CN817274.1|CN817274 HRO4458_D09_H18ZS5 Lib AA070E1X Aven... 268 8e-073
>gb|CN818044.1|CN818044 HRO4472_D08_G16ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4472_D08_G16, mRNA sequence
Length = 842
Score = 388 bits (842), Expect = e-109
Identities = 157/191 (82%), Positives = 173/191 (90%)
Frame = -1 / +1
Query: 574 FQXXVXGSIPVVVGAPNIQEFXPGEGAILHIXXLDDVASVAKTMKNIASNPDAFNQSLRW 395
FQ V G IPVVVGAPNIQEF PGEGAILHI LDDV SVA+TMK+IASNPDAFNQSLRW
Sbjct: 1 FQSLVTGVIPVVVGAPNIQEFSPGEGAILHIKELDDVTSVARTMKHIASNPDAFNQSLRW 180
Query: 394 KYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERTPKFTNRPCSCSTKKGTIYHLF 215
KYDGPSDSFKALIDMAAVHSSCRLCIHIATKIH KEERTPKF NR C+CS+KKG +YHLF
Sbjct: 181 KYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHEKEERTPKFMNRTCNCSSKKGIVYHLF 360
Query: 214 IRERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRI 35
+RERGRFK+ESIY+RS +LTL ALE+AV FRS+NHVP+WKDERP SIRGGD+LK+YRI
Sbjct: 361 VRERGRFKTESIYLRSNKLTLQALETAVHATFRSINHVPIWKDERPSSIRGGDELKVYRI 540
Query: 34 YPVGLTQRQAL 2
YPVGLT+R+AL
Sbjct: 541 YPVGLTEREAL 573
Score = 288 bits (624), Expect = 5e-079
Identities = 132/184 (71%), Positives = 141/184 (76%)
Frame = +3 / -1
Query: 12 RCVRPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSD 191
R VRPTG*ILY F FH GTWL DLN+ TA SKA +VN DL *MLS
Sbjct: 563 RSVRPTG*ILYTFNSSPPRMLDGLSSFHIGTWLMDLNVACTAVSKACKVNLFDLR*MLSV 384
Query: 192 LNRPLSRINKW*IVPFLVEQLQGRFVNFGVLSSFKWILVAICIQRRQDE*TAAMSIRALK 371
LN PLS NKW +PFL+EQL RF+NFGVLSSF I VAIC+QRR DE*TAAMSI ALK
Sbjct: 383 LNLPLSLTNKWYTIPFLLEQLHVRFINFGVLSSFS*IFVAICMQRRHDE*TAAMSISALK 204
Query: 372 ESDGPSYFHLKD*LKASGFEAIFFIVLATEATSSSXLICNIAPSPGXNS*IFGAPTTTGI 551
ESDGPSYFHLKD*LKASGF+AI FIVLATE TSSS L+CNIAPSPG NS*IFGAPTTTG+
Sbjct: 203 ESDGPSYFHLKD*LKASGFDAICFIVLATEVTSSSSLMCNIAPSPGENS*IFGAPTTTGM 24
Query: 552 EPXT 563
P T
Sbjct: 23 TPVT 12
Score = 244 bits (528), Expect = 8e-066
Identities = 108/169 (63%), Positives = 125/169 (73%)
Frame = +2 / -3
Query: 23 TDWVNSV*FQVIPTSNARWPFILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPP 202
T+W+NSV Q+IPTS A WPFILPYRN+V+G K + Q S* *S +NA LKPP
Sbjct: 552 TNWINSVHLQLIPTSYA*WPFILPYRNMVDGSKCGMYSSLQSL*S*FV*S*INAFCLKPP 373
Query: 203 SLSDK*VVNCSLFGGTATRTICKFWSPFLL*MDLGSNMYTKTTR*MNRCHVNKSFERIGW 382
SLS+K*VV S F GT T I KFWSPF M+L S+MY + T *MNRCHVNK ERI W
Sbjct: 372 SLSNK*VVYYSFFAGTVTCAIHKFWSPFFFFMNLRSDMYAEAT**MNRCHVNKCLERIRW 193
Query: 383 TIILPPQRLIEGIRV*SNIFHCLSN*SNIIKLXNM*YCAFSRXKLLNIW 529
TIILPPQRLI+ +R+* N+FHC SN*SN+IKL N+*YC FS KLLNIW
Sbjct: 192 TIILPPQRLIKSVRI*CNMFHCPSN*SNVIKLLNV*YCTFSWRKLLNIW 46
Score = 228 bits (493), Expect = 6e-061
Identities = 114/187 (60%), Positives = 132/187 (70%)
Frame = -3 / +3
Query: 572 SVXSXRFYSGCCWCSKYSRVXSGRRRNITY*XA**CCFSC*DNEKYCFKP*CLQSIFEVE 393
SV R +S C WC+KYSRV S RR NIT+* A** FSC*DNE YC K * SIFEVE
Sbjct: 3 SVTGNRGHSSCRWCTKYSRVFSRRRCNITH*GA**RYFSC*DNETYCIKS*RF*SIFEVE 182
Query: 392 V*WSIRFFQSSY*HGSGSFILSSLYTYCYQDPFKGGKDSKIYKSSL*LFHQKGNNLPLIY 213
V*WSI FFQ +Y*HGSGSFI+S L+TY Y+D K KDSKIY+S +*LF QK N++PLI
Sbjct: 183 V*WSI*FFQGTY*HGSGSFIMSPLHTYRYEDS*KRRKDSKIYESHM*LFQQKRNSIPLIC 362
Query: 212 PRERAV*VREHLHEIRPVNSGSLGIRSAR*I*VPQPRSCMEG*KATEH*RWG*PEIIQNL 33
RER V* R+HL +I+ +N S G I*+ QP S MEG*KA +H RWG* E +QNL
Sbjct: 363 *REREV*DRKHLSKIKQINFTSFGDCCTCHI*IHQPCSYMEG*KAIKHTRWG*VEGVQNL 542
Query: 32 PSRSNAT 12
S S T
Sbjct: 543 SSWSYRT 563
Score = 220 bits (474), Expect = 2e-058
Identities = 114/184 (61%), Positives = 129/184 (70%)
Frame = +1 / -2
Query: 22 DRLGKFCIISGHPHL*CSVAFHPSIQERG*GT*IYRALRIPRLPELTGLISCKCSLT*TA 201
D+L KFC S HPHL C +AFHPSI E G* *++ + P+L +L LI KC L+*T+
Sbjct: 553 DQLDKFCTPSTHPHLVCLMAFHPSI*EHG*WI*MWHVQQSPKLVKLICLILDKCFLS*TS 374
Query: 202 LSLG*ISGKLFPFWWNSYKDDL*ILESFPPLNGSW*QYVYKDDKMNEPLPCQ*EL*KNRM 381
LSL ISG LF F WNSY D *ILESF + S *+YV + D MNEPLPCQ* *KN+M
Sbjct: 373 LSL*QISGILFLFCWNSYMCDS*ILESFLLFHESS*RYVCRGDMMNEPLPCQ*VP*KNQM 194
Query: 382 DHHTSTSKID*RHQGLKQYFSLS*QLKQHHQAX*YVILRLLPEXTLEYLEHQQQPE*NLX 561
DHHTSTSKID*+ Q L QY SLS*QLK HQA * VIL LL E TLEYL HQ+Q E* L
Sbjct: 193 DHHTSTSKID*KRQDLMQYVSLS*QLK*RHQAP*CVILHLLLEKTLEYLVHQRQLE*PLL 14
Query: 562 LXTE 573
TE
Sbjct: 13 PVTE 2
Score = 178 bits (383), Expect(2) = 2e-048
Identities = 84/133 (63%), Positives = 98/133 (73%)
Frame = -2 / +2
Query: 411 INL*GGSMMVHPILSKLLLTWQRFIHLVVFVYILLPRSI*RRKGLQNLQIVLVAVPPKRE 232
INL*GGSMMVH ILS+ LLTWQRFIH V YI L R + ++KGLQNL I V VP K+E
Sbjct: 164 INL*GGSMMVHLILSRHLLTWQRFIHHVASAYISLRRFMKKKKGLQNL*IAHVTVPAKKE 343
Query: 231 QFTTYLSEREGGLSQRAFT*DQAS*LWEPWNPQCSVNLGPSTTFLYGRMKGHRALEVGMT 52
+TTYL EREGGL Q+AF *DQ +*L++ W +L PST FLYGRMKGH+A EVGM+
Sbjct: 344 *YTTYLLEREGGLRQKAFI*DQTN*LYKLWRLLYMPHLDPSTMFLYGRMKGHQAYEVGMS 523
Query: 51 *NYTEFTQSV*RN 13
* TEF Q V +N
Sbjct: 524 *RCTEFIQLVLQN 562
Score = 29.0 bits (57), Expect(2) = 2e-048
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = -2 / +2
Query: 528 QIFKSXLREKAQYY 487
QIFKS L+EK QYY
Sbjct: 47 QIFKSFLQEKVQYY 88
>gb|CN817274.1|CN817274 HRO4458_D09_H18ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4458_D09_H18, mRNA sequence
Length = 542
Score = 268 bits (579), Expect = 8e-073
Identities = 105/133 (78%), Positives = 120/133 (90%)
Frame = -1 / +3
Query: 400 RWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHLKEERTPKFTNRPCSCSTKKGTIYH 221
RWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIH KEERTPKF NR C+CS+KKGT+YH
Sbjct: 3 RWKYDGPSDSFKALIDMAAVHSSCRLCIHIATKIHEKEERTPKFMNRTCNCSSKKGTVYH 182
Query: 220 LFIRERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLY 41
LF RERGR K+ESIY+RS +LTL ALESA+ FRS+NHVPVWKDERP SIRGGD+LK+Y
Sbjct: 183 LFARERGRLKTESIYLRSNELTLEALESAIHATFRSINHVPVWKDERPSSIRGGDELKVY 362
Query: 40 RIYPVGLTQRQAL 2
+IYPVGL + +++
Sbjct: 363 KIYPVGLYRTRSI 401
Score = 189 bits (408), Expect = 3e-049
Identities = 87/127 (68%), Positives = 93/127 (73%)
Frame = +3 / -2
Query: 21 RPTG*ILYNFRXXXXXXXXXXXXFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSDLNR 200
RPTG*ILY F FHTGTWL DLN+ ADSKA +VN DL *MLS L+
Sbjct: 382 RPTG*ILYTFNSSPPRMLDGLSSFHTGTWLMDLNVACIADSKASKVNSFDLR*MLSVLSL 203
Query: 201 PLSRINKW*IVPFLVEQLQGRFVNFGVLSSFKWILVAICIQRRQDE*TAAMSIRALKESD 380
PLS NKW VPFL+EQL RF+NFGVLSSF I VAIC+QRRQDE*TAAMSI ALKESD
Sbjct: 202 PLSLANKWYTVPFLLEQLHVRFINFGVLSSFS*IFVAICMQRRQDE*TAAMSISALKESD 23
Query: 381 GPSYFHL 401
GPSYFHL
Sbjct: 22 GPSYFHL 2
Score = 173 bits (373), Expect = 2e-044
Identities = 77/126 (61%), Positives = 89/126 (70%)
Frame = +2 / -1
Query: 23 TDWVNSV*FQVIPTSNARWPFILPYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPP 202
T+W+N V Q++P+S A WPFILPYRN+V+G K + Q S* *S +NA LKPP
Sbjct: 380 TNWINFVHLQLVPSSYA*WPFILPYRNMVDGSKCGMYSRLQSF*S*FI*S*INAFCLKPP 201
Query: 203 SLSDK*VVNCSLFGGTATRTICKFWSPFLL*MDLGSNMYTKTTR*MNRCHVNKSFERIGW 382
SLS K*VV CS F GT T I KFWSPF M+L S+MY +TT *MNRCHVNK ERI W
Sbjct: 200 SLSSK*VVYCSFFAGTVTCAIHKFWSPFFFFMNLCSDMYAETTG*MNRCHVNKCLERIRW 21
Query: 383 TIILPP 400
TIILPP
Sbjct: 20 TIILPP 3
Score = 166 bits (357), Expect = 3e-042
Identities = 78/126 (61%), Positives = 92/126 (73%)
Frame = -2 / +1
Query: 399 GGSMMVHPILSKLLLTWQRFIHLVVFVYILLPRSI*RRKGLQNLQIVLVAVPPKREQFTT 220
GGSMMVH ILS+ LLTWQRFIH VV YI L R + ++KGLQNL I V VP K+EQ+TT
Sbjct: 4 GGSMMVHLILSRHLLTWQRFIHPVVSAYISLQRFMKKKKGLQNL*IAHVTVPAKKEQYTT 183
Query: 219 YLSEREGGLSQRAFT*DQAS*LWEPWNPQCSVNLGPSTTFLYGRMKGHRALEVGMT*NYT 40
YL EREGGL Q+AF *DQ +*L + W+ +L PST FLYGRMKGH+A E G +* T
Sbjct: 184 YLLEREGGLRQKAFI*DQMN*L*KLWSLLYMPHLDPSTMFLYGRMKGHQAYEEGTS*RCT 363
Query: 39 EFTQSV 22
+F Q V
Sbjct: 364 KFIQLV 381
Score = 151 bits (325), Expect = 8e-038
Identities = 76/126 (60%), Positives = 91/126 (72%)
Frame = -3 / +2
Query: 398 VEV*WSIRFFQSSY*HGSGSFILSSLYTYCYQDPFKGGKDSKIYKSSL*LFHQKGNNLPL 219
VEV*WSI FFQ +Y*HGSGSFILSSL+TY Y+D K KDSKIY+S +*LF QK N++PL
Sbjct: 5 VEV*WSI*FFQGTY*HGSGSFILSSLHTYRYKDS*KRRKDSKIYESHM*LFQQKRNSIPL 184
Query: 218 IYPRERAV*VREHLHEIRPVNSGSLGIRSAR*I*VPQPRSCMEG*KATEH*RWG*PEIIQ 39
I RER * R+HL +I+ +N S G+ I*+ QP SCMEG*KA +H R G E +Q
Sbjct: 185 IC*REREA*DRKHLSKIK*INFRSFGVCYTCHI*IHQPCSCMEG*KAIKHTRRGRVEGVQ 364
Query: 38 NLPSRS 21
NL S S
Sbjct: 365 NLSSWS 382
Score = 149 bits (319), Expect = 6e-037
Identities = 74/126 (58%), Positives = 88/126 (69%)
Frame = +1 / -3
Query: 22 DRLGKFCIISGHPHL*CSVAFHPSIQERG*GT*IYRALRIPRLPELTGLISCKCSLT*TA 201
D+L KFC S P L C +AFHPSIQE G* *++ + P+L +L LI KC L+* +
Sbjct: 381 DQLDKFCTPSTRPLLVCLMAFHPSIQEHG*WI*MWHV*QTPKLLKLIHLILDKCFLS*AS 202
Query: 202 LSLG*ISGKLFPFWWNSYKDDL*ILESFPPLNGSW*QYVYKDDKMNEPLPCQ*EL*KNRM 381
LSL ISG LF F WNSY D *ILESF + S *+YV +DD+MNEPLPCQ* *KN+M
Sbjct: 201 LSL*QISGILFLFCWNSYMCDS*ILESFLLFHESL*RYVCRDDRMNEPLPCQ*VP*KNQM 22
Query: 382 DHHTST 399
DHHTST
Sbjct: 21 DHHTST 4
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,543,417
Number of Sequences: 8143
Number of extensions: 86156
Number of successful extensions: 865
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 207106289
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)