TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QBE1h10.xg.2.1
(463 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816688.1|CN816688 HRO4523_F05_L09ZS5 Lib AA071E1X Aven... 127 2e-030
>gb|CN816688.1|CN816688 HRO4523_F05_L09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4523_F05_L09, mRNA sequence
Length = 632
Score = 127 bits (271), Expect = 2e-030
Identities = 52/62 (83%), Positives = 56/62 (90%)
Frame = +2 / +1
Query: 194 IDRSXVESFGAGGKTCILSRVYPSIAVGKDAHLYVFNNGEXDVTXSGLTAWEMKKPLMNG 373
IDRS VESFGAGGKTCILSRVYPS+A+GKDAHL+VFNNG D+ S LTAWEMKKPLMNG
Sbjct: 4 IDRSVVESFGAGGKTCILSRVYPSMALGKDAHLHVFNNGATDIKVSRLTAWEMKKPLMNG 183
Query: 374 A* 379
A*
Sbjct: 184 A* 189
Score = 101 bits (216), Expect = 6e-023
Identities = 45/61 (73%), Positives = 47/61 (77%)
Frame = -2 / -1
Query: 375 APFIXGFFISQAVRPXXVTSXSPLLKT*R*ASFPTAMDGYTRDRMHVLPPAPKLSTXDRS 196
APFI GFFIS AVR + S +PLL T*R ASFP AMDGYT DRM V PPAPKLST DRS
Sbjct: 185 APFINGFFISHAVRRDTLISVAPLLNT*RCASFPNAMDGYTLDRMQVFPPAPKLSTTDRS 6
Query: 195 I 193
I
Sbjct: 5 I 3
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 3,374,604
Number of Sequences: 8143
Number of extensions: 29787
Number of successful extensions: 1540
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 41
effective length of database: 1,233,624
effective search space used: 138165888
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)