TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAW3a11.yg.2.1
(969 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Aven... 423 e-119
gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Aven... 332 4e-092
gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Aven... 172 8e-044
gb|CN815983.1|CN815983 HRO4515_F02_L03ZS5 Lib AA071E1X Aven... 87 4e-032
gb|CN814825.1|CN814825 HRO4502_H06_O12ZS5 Lib AA071E1X Aven... 88 2e-029
>gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4423_D02_H03, mRNA
sequence
Length = 733
Score = 423 bits (917), Expect = e-119
Identities = 161/185 (87%), Positives = 166/185 (89%)
Frame = +1 / +1
Query: 25 KLXXXLFXXXDIVVXXDLTXXWDVDLNGKVNXXXXXCGXSFHRFDKYLNFSNPHIARNFD 204
KL LF DIVV DLT WDVDL GKVN CG SFHRFDKYLNFSNPHIARNFD
Sbjct: 13 KLDKILFLDDDIVVQKDLTGLWDVDLKGKVNGAVETCGESFHRFDKYLNFSNPHIARNFD 192
Query: 205 PNACGWAYGMNIFDLREWKKKDITGIYHKWQNMNEDRVLWKLGTLPPGLLTFYKLTHPLD 384
PNACGWAYGMNIFDL++WK KDITGIYHKWQNMNEDRVLWKLGTLPPGL+TFYKLTHPLD
Sbjct: 193 PNACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLD 372
Query: 385 KSWHVLGLGYNPSIDRSEIDNAAVVHYNGNMKPWLELAMTKYRPYWTKYIKYDHPYIRGC 564
KSWHVLGLGYNPSIDRSEIDNAAV HYNGNMKPWLELAMTKYRPYWT+YIKYDHPYIRGC
Sbjct: 373 KSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGC 552
Query: 565 NLSE* 579
NLS+*
Sbjct: 553 NLSD* 567
Score = 313 bits (677), Expect = 4e-086
Identities = 138/183 (75%), Positives = 144/183 (78%)
Frame = -2 / -3
Query: 575 SLKLHPRM*G*SYLIYLVQYGRYLVIASSSHGFMFPL**TTAALSISERSMLGLYPNPRT 396
SLKLHPR+*G*SY IYLVQYGRYLVIASS+HGF+FPL** TAALSISERSMLGLYPNPRT
Sbjct: 563 SLKLHPRI*G*SYFIYLVQYGRYLVIASSNHGFIFPL**ATAALSISERSMLGLYPNPRT 384
Query: 395 CHDLSSGCVNL*KVKRPGGSVPSFQRTLSSFIFCHLW*IPVISFFFHSLRSKMFIP*AQP 216
CHDLS GCV+L* V RPGGSVPSFQRTLSSF+FCHLW*IPVIS FFH RSKMFIP*AQP
Sbjct: 383 CHDLSRGCVSL*NVMRPGGSVPSFQRTLSSFMFCHLW*IPVISLFFHCFRSKMFIP*AQP 204
Query: 215 HAFGSKFRAMCGFEXXXXXXXXXXXXPQXXXXXLTFPLRSTSHXXVKSXXTTMSXXXKXX 36
A GSKFRA+ GFE PQ LTFP RSTSH VKS TTMS K
Sbjct: 203 QALGSKFRAI*GFEKLRYLSKRWKLSPQVSTAPLTFPFRSTSHNPVKSFCTTMSSSRKSI 24
Query: 35 XSN 27
SN
Sbjct: 23 LSN 15
Score = 285 bits (616), Expect = 1e-077
Identities = 123/187 (65%), Positives = 142/187 (75%)
Frame = +3 / +3
Query: 27 VGXXXLPXX*HSCXXXFNXXVGC*S*WKGQXXXXXLWXEFSPF*QIS*FFKSTHCSEL*S 206
VG P *HSC F+ +GC*S KGQ LW EF PF*Q+S*FFKS++CSEL*S
Sbjct: 15 VG*DTFPR**HSCAERFDRIMGC*SKRKGQWCSGDLWREFPPF*QVS*FFKSSYCSEL*S 194
Query: 207 KCMWLGLWDEHL*SEGVEEERYYWDLPQMAKYE*RQGPLEAWDTSAWPLDLL*VDAPTGQ 386
C+WLGLWDEHL*S+ VEE+RYY DLPQ+AK+E*RQG LEA DTS WP D+L D
Sbjct: 195 *CLWLGLWDEHL*SKAVEEQRYYRDLPQVAKHE*RQGSLEARDTSTWPHDVLQADTSP** 374
Query: 387 VMACSWIRIQPKH*PFRDRQCCCGSLQWEHEAMAGASNDQVPTILDQVYQV*SPLHPWMQ 566
V+AC WIRIQPKH P DRQCCC SLQWE+EAM GA NDQV ILD+VY+V*S L+P MQ
Sbjct: 375 VVACPWIRIQPKHRPLGDRQCCCCSLQWEYEAMVGAGNDQVSAILDKVYKV*SSLYPRMQ 554
Query: 567 LE*VDSR 587
LE*+++R
Sbjct: 555 LE*LETR 575
Score = 277 bits (600), Expect = 2e-075
Identities = 121/184 (65%), Positives = 128/184 (69%)
Frame = -3 / -1
Query: 577 THSSCIHGCKGDHT*YTWSNMVGTWSLLAPAMASCSHCNEPQQHCLSLNGQCLGCILIQE 398
+HSSCI G K DHT YT S+M TWSL AP MAS SHCNE QQHCLS +G CLGCILIQ
Sbjct: 565 SHSSCIRGYKDDHTLYTLSSMADTWSLPAPTMASYSHCNEQQQHCLSPSGLCLGCILIQG 386
Query: 397 HAMTCPVGASTYRRSRGQAEVSQASKGPCLHSYFAICGRSQ*YLSSSTPSDQRCSSHKPS 218
HA T S R S GQ EVS+ASK PCLHS FA CGRS+*YL SST DQRCSSHKP+
Sbjct: 385 HATTYQGDVSACRTS*GQVEVSRASKEPCLHSCFATCGRSR*YLCSSTALDQRCSSHKPN 206
Query: 217 HMHLDQSSEQCVDLKN*DICQNGENSXHRXXXXH*PFH*DQHPTXLLNXXXQLCXXXGRX 38
H H DQSSEQ DLKN*D CQNG NS HR H*PF DQHP L N QLC G+
Sbjct: 205 HKH*DQSSEQYEDLKN*DTCQNGGNSLHRSPLHH*PFLLDQHPIILSNLSAQLCHHRGKV 26
Query: 37 XXPT 26
PT
Sbjct: 25 SYPT 14
Score = 228 bits (493), Expect = 9e-061
Identities = 124/185 (67%), Positives = 128/185 (69%)
Frame = +2 / +2
Query: 26 SWXXXSSXXXT*LXXXI*QXCGMLILMERSMXXXXPVXXVFTVLTNILIFQIHTLLGTLI 205
SW S T*L I*Q GMLI ERSM PV V TVLT+ILIFQI LLGTLI
Sbjct: 14 SWIRYFSSMMT*LCRKI*QDYGMLI*KERSMVQWRPVERVSTVLTSILIFQILILLGTLI 193
Query: 206 QMHVAGLMG*TSLI*GSGRRKILLGSTTNGKI*MKTGSFGSLGHFRLAS*PSIS*RTHWT 385
M V GLMG*TSLI* SGR KIL GSTT+GK *MKTG FGS GHF LAS* S S*
Sbjct: 194 LMLVVGLMG*TSLI*SSGRTKILPGSTTSGKT*MKTGFFGSSGHFHLAS*RSTS*HIPLI 373
Query: 386 SHGMFLD*DTTQALTVQR*TMLLWFITMGT*SHGWS*Q*PSTDHIGPSISSMITLTSVDA 565
S GM LD*DTTQA T +R*TMLL ITMG *SHGWS Q*PS H G I SMI L S DA
Sbjct: 374 SRGMSLD*DTTQA*TARR*TMLLLLITMGI*SHGWSWQ*PSIGHTGQGI*SMIILISADA 553
Query: 566 T*VSR 580
T*V+R
Sbjct: 554 T*VTR 568
Score = 220 bits (475), Expect(2) = 4e-063
Identities = 92/132 (69%), Positives = 110/132 (83%)
Frame = -1 / -2
Query: 582 YLLTQVASTDVRVIILDILGPIWSVLGHC*LQPWLHVPIVMNHSSIVYL*TVNAWVVS*S 403
+L+TQVAS D+R+IIL I P+W +LGHC LQPWLH+PIVM++SSIVYL V AWVVS*S
Sbjct: 570 FLVTQVASADIRMIILYIPCPVWPILGHCQLQPWLHIPIVMSNSSIVYLRAVYAWVVS*S 391
Query: 402 KNMP*LVQWVRQLIEGQEARRKCPKLPKDPVFIHILPFVVDPSNIFLLPLPQIKDVHPIS 223
K+MP L++ + QL+E EAR KCP+LPK+PVFIH+LP VVDP NIF+LPL IKDVHPIS
Sbjct: 390 KDMPRLIKGMCQLVERHEARWKCPELPKNPVFIHVLPLVVDPGNIFVLPLL*IKDVHPIS 211
Query: 222 PATCIWIKVPSN 187
P T I IKVPSN
Sbjct: 210 PTTSIRIKVPSN 175
Score = 36.8 bits (74), Expect(2) = 4e-063
Identities = 18/36 (50%), Positives = 19/36 (52%)
Frame = -1 / -2
Query: 132 GXXXXIDLSIKINIPXXC*IXXHNYVXXXEEXXXQL 25
G IDLS INIP C I HNYV E+ QL
Sbjct: 120 GLHCTIDLSF*INIP*SCQIFLHNYVIIEEKYLIQL 13
>gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_A06_B12, mRNA sequence
Length = 570
Score = 332 bits (720), Expect = 4e-092
Identities = 119/147 (80%), Positives = 137/147 (93%)
Frame = +1 / +1
Query: 145 FHRFDKYLNFSNPHIARNFDPNACGWAYGMNIFDLREWKKKDITGIYHKWQNMNEDRVLW 324
F RFDKYLNFSNP+IA+NFD NACGWAYGMN+FDL EWKKKDITGIYH+WQ +NE+R+LW
Sbjct: 22 FPRFDKYLNFSNPNIAQNFDANACGWAYGMNMFDLEEWKKKDITGIYHRWQTLNENRLLW 201
Query: 325 KLGTLPPGLLTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVVHYNGNMKPWLELAMT 504
KLGTLPPGL+TFYKLTHPLDKSWHVLGLGYNP+++ SEID+AAV+HYNGNMKPWLE+AMT
Sbjct: 202 KLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPTVEHSEIDSAAVIHYNGNMKPWLEIAMT 381
Query: 505 KYRPYWTKYIKYDHPYIRGCNLSE*IV 585
KYRPYWTKYI Y+H Y+ GC +S+* V
Sbjct: 382 KYRPYWTKYINYEHSYVHGCKISQ*TV 462
Score = 223 bits (481), Expect = 4e-059
Identities = 101/137 (73%), Positives = 109/137 (79%)
Frame = -2 / -2
Query: 584 TIYSLKLHPRM*G*SYLIYLVQYGRYLVIASSSHGFMFPL**TTAALSISERSMLGLYPN 405
T+Y L LHP * S L+Y VQ GRY VIA SSHGFMFPL* TAALSISE S +GLYPN
Sbjct: 461 TVY*LILHPWT*ECS*LMYFVQ*GRYFVIAISSHGFMFPL*WMTAALSISECSTVGLYPN 282
Query: 404 PRTCHDLSSGCVNL*KVKRPGGSVPSFQRTLSSFIFCHLW*IPVISFFFHSLRSKMFIP* 225
P TCHDLSSGCV+L*KV RPGGSVPSF +L SF CHLW*IPV+SFFFHS RS +FIP*
Sbjct: 281 PSTCHDLSSGCVSL*KVIRPGGSVPSFHSSLFSFRVCHLW*IPVMSFFFHSSRSNIFIP* 102
Query: 224 AQPHAFGSKFRAMCGFE 174
AQPHA SKF A+ GFE
Sbjct: 101 AQPHALASKFWAIFGFE 51
Score = 165 bits (354), Expect = 1e-041
Identities = 75/145 (51%), Positives = 97/145 (66%)
Frame = +3 / +3
Query: 150 PF*QIS*FFKSTHCSEL*SKCMWLGLWDEHL*SEGVEEERYYWDLPQMAKYE*RQGPLEA 329
P *Q+ FFK +C EL* CMWLGLW+E++*S +EEE +YWDLP MA E* Q +E
Sbjct: 27 PI*QVPQFFKPKYCPEL*C*CMWLGLWNEYV*SGRMEEEGHYWDLP*MADPE*EQAAVET 206
Query: 330 WDTSAWPLDLL*VDAPTGQVMACSWIRIQPKH*PFRDRQCCCGSLQWEHEAMAGASNDQV 509
DTS D L D P GQ+MA +WIRIQP * +RQ C LQ +HE MAG +D+V
Sbjct: 207 GDTSTRSYDFLQADTPAGQIMARAWIRIQPNR*ALGNRQRRCHPLQRKHETMAGDRDDKV 386
Query: 510 PTILDQVYQV*SPLHPWMQLE*VDS 584
P +LD+V+Q+*+ L P +Q + +DS
Sbjct: 387 PALLDKVHQL*AFLCPRVQDQSIDS 461
Score = 152 bits (326), Expect = 1e-037
Identities = 67/131 (51%), Positives = 88/131 (67%)
Frame = -1 / -1
Query: 579 LLTQVASTDVRVIILDILGPIWSVLGHC*LQPWLHVPIVMNHSSIVYL*TVNAWVVS*SK 400
LLT +A D+ ++I+D+L P+ VL H LQPW HV +V++ S V +N WVVS*SK
Sbjct: 456 LLTDLAPVDIGMLIIDVLCPVRPVLCHRDLQPWFHVSVVVDDSGAVDFRVLNGWVVS*SK 277
Query: 399 NMP*LVQWVRQLIEGQEARRKCPKLPKDPVFIHILPFVVDPSNIFLLPLPQIKDVHPISP 220
++P* VQ V QL+E + KCP+ P+ PV I LP +V+PSN+ LLP QIK +H ISP
Sbjct: 276 HVP*FVQRVCQLVESHKTWWKCPQFPQQPVLIQGLPSMVNPSNVLLLPFFQIKHIHSISP 97
Query: 219 ATCIWIKVPSN 187
TCI IKV N
Sbjct: 96 TTCISIKVLGN 64
Score = 139 bits (298), Expect = 7e-034
Identities = 70/140 (50%), Positives = 80/140 (57%)
Frame = -3 / -3
Query: 568 SCIHGCKGDHT*YTWSNMVGTWSLLAPAMASCSHCNEPQQHCLSLNGQCLGCILIQEHAM 389
SC G + H * T S+ GT S +PAM SC C+ Q+ C + Q LGCILIQ AM
Sbjct: 445 SCTRGHRNAHN*CTLSSKAGTLSSRSPAMVSCFRCSG*QRRCRFPSAQRLGCILIQARAM 266
Query: 388 TCPVGASTYRRSRGQAEVSQASKGPCLHSYFAICGRSQ*YLSSSTPSDQRCSSHKPSHMH 209
CP G S R+S EVS S C HS AI G+SQ* SSS DQ S HKP+HMH
Sbjct: 265 ICPAGVSACRKS*DLVEVSPVSTAACSHSGSAIYGKSQ*CPSSSILPDQTYSFHKPNHMH 86
Query: 208 LDQSSEQCVDLKN*DICQNG 149
QSS Q + LKN* CQ G
Sbjct: 85 *HQSSGQYLGLKN*GTCQIG 26
Score = 122 bits (262), Expect = 7e-029
Identities = 69/142 (48%), Positives = 84/142 (59%)
Frame = +2 / +2
Query: 155 LTNILIFQIHTLLGTLIQMHVAGLMG*TSLI*GSGRRKILLGSTTNGKI*MKTGSFGSLG 334
LT+ IFQ L TL+ MHV GLM * LI +GRR+ LLG T +G+ *M+TG G+ G
Sbjct: 32 LTSTSIFQTQILPRTLMLMHVVGLME*ICLIWKNGRRRTLLGFTIDGRP*MRTGCCGNWG 211
Query: 335 HFRLAS*PSIS*RTHWTSHGMFLD*DTTQALTVQR*TMLLWFITMGT*SHGWS*Q*PSTD 514
HF * S S* T WT+HG LD*DTTQ L+ ++ T L T T*+HGW +* ST
Sbjct: 212 HFHQVL*LSTS*HTRWTNHGTCLD*DTTQPLSTRKSTAPLSSTTTET*NHGWRSR*QSTG 391
Query: 515 HIGPSISSMITLTSVDAT*VSR 580
G S S M S A V+R
Sbjct: 392 LTGQSTSIMSIPMSTGARSVNR 457
>gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_D01_H01, mRNA sequence
Length = 653
Score = 172 bits (370), Expect = 8e-044
Identities = 63/93 (67%), Positives = 74/93 (79%)
Frame = +1 / +1
Query: 295 QNMNEDRVLWKLGTLPPGLLTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVVHYNGN 474
Q NE+R+LWKLGTLPPGL+TFY T PLDKSWHVLGLGYNPSI EI NAAVVH+NGN
Sbjct: 331 QTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGN 510
Query: 475 MKPWLELAMTKYRPYWTKYIKYDHPYIRGCNLS 573
MKPWL++ M ++R WTKY+ Y +IR CN +
Sbjct: 511 MKPWLDIGMNQFRHLWTKYVDYGDSFIRQCNFA 609
>gb|CN815983.1|CN815983 HRO4515_F02_L03ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_F02_L03, mRNA sequence
Length = 668
Score = 87.2 bits (184), Expect(3) = 4e-032
Identities = 35/97 (36%), Positives = 58/97 (59%)
Frame = +1 / +1
Query: 307 EDRVLWKLGTLPPGLLTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVVHYNGNMKPW 486
+D V + LL+F L +PLD+ + GLGY+ ID ++A +HYNGNMKPW
Sbjct: 343 KDEVALREAAFSISLLSFQHLIYPLDERLILSGLGYDYGIDPEVARSSASLHYNGNMKPW 522
Query: 487 LELAMTKYRPYWTKYIKYDHPYIRGCNLSE*IVVLKN 597
LEL + +Y+ YW +++ + ++ CN++ * ++N
Sbjct: 523 LELGILEYKKYWRRFLTREDRFMDECNVNP*HT*IRN 633
Score = 43.2 bits (88), Expect(3) = 4e-032
Identities = 14/46 (30%), Positives = 25/46 (54%)
Frame = +1 / +1
Query: 1 FYLPXVYPKLXXXLFXXXDIVVXXDLTXXWDVDLNGKVNXXXXXCG 138
F++P ++ L + D+V+ DL+ W++D+ KVN CG
Sbjct: 49 FFIPEIFKDLKKVVVLDDDVVIQRDLSFLWNLDMGDKVNGAVEFCG 186
Score = 40.9 bits (83), Expect(3) = 4e-032
Identities = 12/29 (41%), Positives = 19/29 (65%)
Frame = +1 / +1
Query: 199 FDPNACGWAYGMNIFDLREWKKKDITGIY 285
+DP +C W G+N+ +L +WKK +T Y
Sbjct: 229 YDPKSCAWKSGVNVINLDKWKKHKVTEKY 315
>gb|CN814825.1|CN814825 HRO4502_H06_O12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_H06_O12, mRNA sequence
Length = 837
Score = 88.1 bits (186), Expect(3) = 2e-029
Identities = 37/110 (33%), Positives = 61/110 (55%)
Frame = +1 / +1
Query: 307 EDRVLWKLGTLPPGLLTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVVHYNGNMKPW 486
+D V + LL+F L +PLD+ + GLGY+ ID ++A +HYNGNMKPW
Sbjct: 280 KDEVALREAAFSISLLSFQHLIYPLDERLTLSGLGYDYGIDPEVARSSASLHYNGNMKPW 459
Query: 487 LELAMTKYRPYWTKYIKYDHPYIRGCNLSE*IVVLKN*QMRKSLLDRSVP 636
LEL + +Y+ YW +++ + ++ CN + * ++N + + D P
Sbjct: 460 LELGILEYKKYWRRFLTREDRFMDECNANP*HT*IRNQNI*RQFCDTHQP 609
Score = 39.6 bits (80), Expect(3) = 2e-029
Identities = 11/29 (37%), Positives = 19/29 (65%)
Frame = +1 / +1
Query: 199 FDPNACGWAYGMNIFDLREWKKKDITGIY 285
+DP +C W G+N+ +L +W+K +T Y
Sbjct: 166 YDPKSCAWMSGVNVINLDKWRKHKVTENY 252
Score = 34.5 bits (69), Expect(3) = 2e-029
Identities = 11/28 (39%), Positives = 17/28 (60%)
Frame = +1 / +1
Query: 55 DIVVXXDLTXXWDVDLNGKVNXXXXXCG 138
D+V+ DL+ W++D+ KVN CG
Sbjct: 40 DVVIQRDLSFLWNLDMGDKVNGAVEFCG 123
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,195,640
Number of Sequences: 8143
Number of extensions: 166708
Number of successful extensions: 1077
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 339637302
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)