TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAN11a10.yg.3.1
(1341 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818669.1|CN818669 HRO4467_F09_K17ZS5 Lib AA070E1X Aven... 513 e-146
gb|CN817711.1|CN817711 HRO4461_F03_L05ZS5 Lib AA070E1X Aven... 171 2e-043
>gb|CN818669.1|CN818669 HRO4467_F09_K17ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4467_F09_K17, mRNA sequence
Length = 885
Score = 513 bits (1114), Expect = e-146
Identities = 204/233 (87%), Positives = 215/233 (92%)
Frame = +2 / +3
Query: 356 CNDAGRSVMERYTKMSNAMKTYGKGIFFSLCEWGRQNPATWAGGMGNSWRTTDDIADNWG 535
CNDAGRSVM+RYTKMS AMK YGK IFFSLCEWG +NPATWA GMGNSWRTT DIADNWG
Sbjct: 12 CNDAGRSVMDRYTKMSKAMKKYGKNIFFSLCEWGNENPATWARGMGNSWRTTGDIADNWG 191
Query: 536 SMTSRADQNDRWASYAGPGGWNDPDMLEVGNGGMSEAEYRSHFSIWALAKAPLLIGCDVR 715
SMTSRADQNDRWASYAGPGGWNDPDMLEVGNGGM+EAEYRSHFSIWALAKAPLLIGCDVR
Sbjct: 192 SMTSRADQNDRWASYAGPGGWNDPDMLEVGNGGMTEAEYRSHFSIWALAKAPLLIGCDVR 371
Query: 716 AMSQQTMGILSNSEVIAVNQDSLGVQGKKVQSSNGLEVWAGPLSNNRKAVVLWNRQGYQA 895
+MSQQT I+SN EVI VNQD LGVQGKKVQS+NGLEVWAGPL+ NRKAVVLWNRQGYQA
Sbjct: 372 SMSQQTKEIISNWEVIGVNQDGLGVQGKKVQSNNGLEVWAGPLTGNRKAVVLWNRQGYQA 551
Query: 896 TITAQWSSIGLPSSTAVTARDLWAHSSFSAQGQLSASVPPHDCKMYVLTPN*L 1054
TITA WSS+GLP+S AVT RDLWAHSS SAQGQ+SASV PHDCKMY+LTP *L
Sbjct: 552 TITAYWSSVGLPASAAVTVRDLWAHSSSSAQGQISASVAPHDCKMYILTPK*L 710
Score = 429 bits (931), Expect = e-121
Identities = 186/242 (76%), Positives = 198/242 (81%)
Frame = -1 / -3
Query: 1080 HGTIRSCLLS*FGVKTYILQS*GGTEADSWP*AENDECAHRSRAVTAVEEGSPMLDHCAV 901
+G + + S FGV+ YILQS*G TEAD P*AE+DECAHRSR VTA + GSP LD AV
Sbjct: 736 YGLHSAFVYSYFGVRMYILQS*GATEADICP*AEDDECAHRSRTVTAADAGSPTLDQYAV 557
Query: 900 MVAWYPCRFQRTTAFLLLLSGPAQTSKPLLDCTFFPCTPRLSWFTAMTSELLSMPIVCWL 721
MVAWYPC F RTTAFL + GPA TSKPLLDCTFFP TPR SW T MTS+LL + VCWL
Sbjct: 556 MVAWYPCLFHRTTAFLFPVRGPAHTSKPLLDCTFFPWTPRPSWLTPMTSQLLIISFVCWL 377
Query: 720 IARTSHPIRSGALASAQMLK*ERYSASDMPPLPTSSMSGSFHPPGPA*EAHLSFWSARDV 541
I RTSHPIR GALASAQMLK ERYSAS +PP PTSSMSGSFHPPGPA*EAHLSFWSARDV
Sbjct: 376 IERTSHPIRRGALASAQMLKCERYSASVIPPFPTSSMSGSFHPPGPA*EAHLSFWSARDV 197
Query: 540 MLPQLSAISSVVLQLFPMPPAQVAGFCLPHSHSEKKMPFPYVFIALLIFVYLSMTLLPAS 361
MLPQLSA+S VVLQL PMP A VAGF PHS SEKKM FPY F+ALLI VYLSMTLLPAS
Sbjct: 196 MLPQLSAMSPVVLQLLPMPRAHVAGFSFPHSQSEKKMFFPYFFMALLILVYLSMTLLPAS 17
Query: 360 LQ 355
LQ
Sbjct: 16 LQ 11
Score = 281 bits (608), Expect = 2e-076
Identities = 131/241 (54%), Positives = 154/241 (63%)
Frame = -3 / -2
Query: 1078 WNNPLLSSELVRRQDIHLAVVRWHRS**LALSRE*RVRP*ITSSDSSGRREPDARPLRGD 899
W +L+ +D+HLAV+R HR L LS *RVRP*I +SD S R+P+A P+ D
Sbjct: 734 WTPLCFRLQLLWCEDVHLAVMRCHRGGYLPLSGR*RVRP*IANSDGS*CRKPNA*PICSD 555
Query: 898 GCLVSLPVPENHGLPVVAERPSPNLQAIAGLHFLSLHA*AILVHGDDFRVAEYXXXXXXX 719
GCLV LPVP NH PV +ER P LQ I LH LSL A*AILV DD VA+Y
Sbjct: 554 GCLVPLPVP*NHSFPVPSERTGPYLQTIIRLHLLSLDA*AILVDADDLPVADYFLCLLAH 375
Query: 718 XXHVAPDQKRSLGERPDAEVRAVLRLGHAAVAHLKHVRIVPPARSGVGSPPVVLVCTRRH 539
H+APDQKRSLG+ PDAEVRAVL H + + KHVRIVP RS VG P VVLV H
Sbjct: 374 RAHIAPDQKRSLGKCPDAEVRAVLSFSHPTIPNFKHVRIVPSTRSRVGGPSVVLVRAGCH 195
Query: 538 AAPVVGDIIGRSPAVPHAACPGSRVLPPPFAQREEDALPIRLHSVAHLRIPLHDAPAGVV 359
AAPVVG++ PA+ HA CP SRVL PF + EED LPI LH AHL + +HD ++
Sbjct: 194 AAPVVGNVASCPPAITHATCPCSRVLVSPFTE*EEDVLPIFLHGFAHLSVSIHDTSTSII 15
Query: 358 A 356
A
Sbjct: 14 A 12
Score = 216 bits (467), Expect = 5e-057
Identities = 100/194 (51%), Positives = 117/194 (60%)
Frame = -2 / -1
Query: 1031 TSCSREVAPKLIAGLEPRMTSAPIDHEQ*QQWKKGARCSTTAR*WLPGIPAGSREPRPSC 852
TSCS EV P+ I+ E +MTSAPID EQ*+Q + A+ T +*WLPG PA S EP+ SC
Sbjct: 687 TSCSHEVPPRRISAPERKMTSAPIDREQ*RQLMQEAQRLTNMQ*WLPGTPACSIEPQLSC 508
Query: 851 CC*AAQPKPPSHCWTALSFPARLGYLGSRR*LPSC*VCPXXXXXXXXXXXXXXAEPWRAP 672
* +P PP+H TA SFP RLG+LG RR* PSC*+ P EPW+ P
Sbjct: 507 SQ*EDRPIPPNHY*TAPSFPGRLGHLG*RR*PPSC*LFPLSAGSSSAHRTRSEEEPWQVP 328
Query: 671 RC*SESGXXXXXXXXXXPQACQDRSTRQVRRRKPTCRSGLHETSCCPSCRRYHRSFSSCS 492
RC*S SG QACQDRS QV RR+P CRSG SCCPSCR+ + SS
Sbjct: 327 RC*SASGTQLQSSHHSQLQACQDRSIHQVPRRRPICRSGPRGMSCCPSCRQCRQLSSSYY 148
Query: 491 PCRLPR*QGFASPI 450
PC +P *QG PI
Sbjct: 147 PCHVPM*QGSRFPI 106
Score = 206 bits (445), Expect = 5e-054
Identities = 105/216 (48%), Positives = 119/216 (55%)
Frame = +3 / +1
Query: 432 SSSRCANGGGKTLLPGQAAWGTAGERPMISPTTGAA*RLVQTRTTGGLPTPDLAGGTILT 611
SSS NG +TLL G AW AG + PTTGAA* +TRTT G PT DL GTILT
Sbjct: 88 SSSHSVNGETRTLLHGHVAWVIAGGQLATLPTTGAA*HPARTRTTDGPPTRDLVDGTILT 267
Query: 612 CLRWATAACPRRSTALTSASGRSPRLRF*SGATCXXXXXXXXXXXXXXKSSP*TKIA*AC 791
CL+ + STA TSASG PRL F*SGA C +SS TK+A*A
Sbjct: 268 CLKLGMVG*LKLSTARTSASGHLPRLLF*SGAMCAR*ASRQRK*SATGRSSASTKMA*AS 447
Query: 792 RERKCSPAMAWRFGLGRSATTGRPWFSGTGRDTRQPSPRSGRAXXXXXXXXXXXVIYGRT 971
RER+CS M WR+G S TG+ WF GTGR TRQPS G+A IYGRT
Sbjct: 448 RERRCSLIMVWRYGPVLSLGTGKLWFYGTGRGTRQPSLHIGQALGFLHQLPSLFAIYGRT 627
Query: 972 RHSRLKASYQLRCHLTTARCMS*RRTNSEDKSGLFH 1079
RH L+ Y R HL TARC S ++N K H
Sbjct: 628 RHLPLRGRYPPRWHLMTARCTSSHQSNCRRKQSGVH 735
Score = 108 bits (230), Expect(3) = 5e-062
Identities = 44/79 (55%), Positives = 59/79 (74%)
Frame = +1 / +2
Query: 379 HGEVYEDEQRYEDVWEGHLLLAVRMGEAKPCYLGRRHGEQLENDR*YRRQLGQHDVSCRP 558
HG ++ DEQ +E++WE HLLL + MG+ +PCY+G HG LE++ + RQLGQHD+ P
Sbjct: 35 HG*IH*DEQSHEEIWEEHLLLTL*MGKREPCYMGTWHG**LEDNWRHCRQLGQHDIPRGP 214
Query: 559 ERQVGFLRRTWRVERS*HA 615
ERQ+G LR TW +ERS*HA
Sbjct: 215 ERQMGLLRGTWWMERS*HA 271
Score = 93.2 bits (197), Expect(3) = 5e-062
Identities = 39/53 (73%), Positives = 43/53 (81%)
Frame = +1 / +2
Query: 652 PLSLQHLGARQGSASDRVRRARDEPADDGHTQQLGSHRREPR*PRRAGKESAV 810
PL+LQHLG QGS+SDRVR A DEPAD G+ QQLG HRR+PR PRR GKE AV
Sbjct: 308 PLALQHLGTCQGSSSDRVRCALDEPADKGNNQQLGGHRRQPRWPRRPGKEGAV 466
Score = 71.2 bits (149), Expect(3) = 5e-062
Identities = 33/59 (55%), Positives = 37/59 (62%)
Frame = +1 / +2
Query: 856 EGRGSLEPAGIPGNHHRAVVEHRAPFFHCCHCS*SMGALVILGSRPAISFGATSRLQDV 1032
E GS+E AG+PGNHH +V+ A C HCS SMGALVI S I G TS LQDV
Sbjct: 512 ESCGSMEQAGVPGNHHCILVKRWASCISCRHCSRSMGALVIFRSGADIRLGGTS*LQDV 688
>gb|CN817711.1|CN817711 HRO4461_F03_L05ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4461_F03_L05, mRNA sequence
Length = 528
Score = 171 bits (368), Expect = 2e-043
Identities = 67/83 (80%), Positives = 76/83 (91%)
Frame = +2 / +2
Query: 806 QSSNGLEVWAGPLSNNRKAVVLWNRQGYQATITAQWSSIGLPSSTAVTARDLWAHSSFSA 985
+++NGLEVWAGPL+ +RKAVVLWNRQGYQATITA WSS+GLP+S AVT RDLWAHSS SA
Sbjct: 8 EANNGLEVWAGPLTGDRKAVVLWNRQGYQATITAYWSSVGLPASAAVTVRDLWAHSSSSA 187
Query: 986 QGQLSASVPPHDCKMYVLTPN*L 1054
QGQ+SASV PHDCKMY+LTP *L
Sbjct: 188 QGQISASVAPHDCKMYILTPK*L 256
Score = 138 bits (297), Expect = 1e-033
Identities = 61/90 (67%), Positives = 68/90 (75%)
Frame = -1 / -1
Query: 1080 HGTIRSCLLS*FGVKTYILQS*GGTEADSWP*AENDECAHRSRAVTAVEEGSPMLDHCAV 901
+G + + S FGV+ YILQS*G TEAD P*AE+DECAHRSR VTA + GSP LD AV
Sbjct: 282 YGLHSAFVYSYFGVRMYILQS*GATEADICP*AEDDECAHRSRTVTAADAGSPTLDQYAV 103
Query: 900 MVAWYPCRFQRTTAFLLLLSGPAQTSKPLL 811
MVAWYPC F RTTAFL + GPA TSKPLL
Sbjct: 102 MVAWYPCLFHRTTAFLSPVRGPAHTSKPLL 13
Score = 97.7 bits (207), Expect = 4e-021
Identities = 47/91 (51%), Positives = 57/91 (62%)
Frame = -3 / -3
Query: 1078 WNNPLLSSELVRRQDIHLAVVRWHRS**LALSRE*RVRP*ITSSDSSGRREPDARPLRGD 899
W +L+ +D+HLAV+R HR L LS *RVRP*I +SD S R+P+A P+ D
Sbjct: 280 WTPLCFRLQLLWCEDVHLAVMRCHRGGYLPLSGR*RVRP*IANSDGS*CRKPNA*PICSD 101
Query: 898 GCLVSLPVPENHGLPVVAERPSPNLQAIAGL 806
GCLV LPVP NH PV +ER P LQ I L
Sbjct: 100 GCLVPLPVP*NHSFPVPSERTGPYLQTIISL 8
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 16,278,060
Number of Sequences: 8143
Number of extensions: 247667
Number of successful extensions: 1565
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 486096390
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)