TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAE12f02.yg.2.1
(290 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Aven... 136 1e-033
gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Aven... 129 1e-031
gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Aven... 107 7e-025
>gb|CN815813.1|CN815813 HRO4510_G06_M12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4510_G06_M12, mRNA sequence
Length = 521
Score = 136 bits (291), Expect = 1e-033
Identities = 55/94 (58%), Positives = 64/94 (68%)
Frame = +3 / +2
Query: 9 KWXXXXXXXXXXXXXNFIGVVAGVSNAINNGYESWGPXFGKLFFAFWVIXXLYPFLKGLV 188
+W N +G+VAGVS+A+NNGY SWGP FGKLFF+FWVI LYPFLKGL+
Sbjct: 11 QWTTLLIPPTTLIIINMVGIVAGVSDAVNNGYGSWGPLFGKLFFSFWVIVHLYPFLKGLM 190
Query: 189 GRQNRTPTIXIXXXILLASIFSXXXXXIXPXXAK 290
GRQNRTPTI + +LLASIFS I P AK
Sbjct: 191 GRQNRTPTIVVLWSVLLASIFSLVWVRIDPFIAK 292
Score = 107 bits (229), Expect = 5e-025
Identities = 49/92 (53%), Positives = 54/92 (58%)
Frame = -2 / -3
Query: 286 AXXGSXXXXXXEKIEASRMXXXXXIVGVLFCLPTKPLRKGYXXXITQNAKKSFPXXGPHD 107
A GS EK+EASR +VGV FCLP PLR G +TQ KKSFP GPHD
Sbjct: 288 AMKGSILTQTREKMEASRTDQSTTMVGVRFCLPMSPLRNG*RWTMTQKEKKSFPKRGPHD 109
Query: 106 SYPLLIALETPATTPMKFNRSKVVGGINKVVH 11
PLL A ETPAT P VVGGI++VVH
Sbjct: 108 P*PLLTASETPATMPTMLMMMSVVGGISRVVH 13
>gb|CN819884.1|CN819884 HRO4407_C09_F17ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C09_F17, mRNA
sequence
Length = 676
Score = 129 bits (277), Expect = 1e-031
Identities = 54/79 (68%), Positives = 59/79 (74%)
Frame = +3 / +1
Query: 54 NFIGVVAGVSNAINNGYESWGPXFGKLFFAFWVIXXLYPFLKGLVGRQNRTPTIXIXXXI 233
N +GVVAG S AIN+GY+SWGP FGKLFFAFWVI LYPFLKGL+GR NRTPTI I I
Sbjct: 49 NMVGVVAGTSYAINSGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAI 228
Query: 234 LLASIFSXXXXXIXPXXAK 290
LLASIFS I P +
Sbjct: 229 LLASIFSLLWVRIDPFTTR 285
Score = 102 bits (217), Expect = 2e-023
Identities = 48/87 (55%), Positives = 52/87 (59%)
Frame = -2 / -3
Query: 277 GSXXXXXXEKIEASRMXXXXXIVGVLFCLPTKPLRKGYXXXITQNAKKSFPXXGPHDSYP 98
GS EKIEA R+ IVGV F LP +PLR GY ITQ AKKSFP GPHD P
Sbjct: 272 GSIRTHSKEKIEARRIAQTMTIVGVRFVLPIRPLRNGYK*TITQKAKKSFPNSGPHDW*P 93
Query: 97 LLIALETPATTPMKFNRSKVVGGINKV 17
LL+A E PATTP VVGG+ V
Sbjct: 92 LLMA*EVPATTPTMLMIKIVVGGMRSV 12
>gb|CN819594.1|CN819594 HRO4401_H12_O23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4401_H12_O23, mRNA
sequence
Length = 620
Score = 107 bits (228), Expect = 7e-025
Identities = 44/65 (67%), Positives = 48/65 (73%)
Frame = +3 / +2
Query: 96 NGYESWGPXFGKLFFAFWVIXXLYPFLKGLVGRQNRTPTIXIXXXILLASIFSXXXXXIX 275
+GY+SWGP FGKLFFAFWVI LYPFLKGL+GR NRTPTI I ILLASIFS I
Sbjct: 2 SGYQSWGPLFGKLFFAFWVIVHLYPFLKGLMGRTNRTPTIVIVWAILLASIFSLLWVRID 181
Query: 276 PXXAK 290
P +
Sbjct: 182 PFTTR 196
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 2,702,223
Number of Sequences: 8143
Number of extensions: 28171
Number of successful extensions: 2759
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 39
effective length of database: 1,249,910
effective search space used: 71244870
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)