TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= QAD10d05.sg.3.3
         (1563 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN815423.1|CN815423  HRO4509_E05_I09ZS5 Lib AA071E1X Aven...   178   2e-045

>gb|CN815423.1|CN815423 HRO4509_E05_I09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
            sativa cDNA clone HRO4509_E05_I09, mRNA sequence
          Length = 649

 Score =  178 bits (383), Expect = 2e-045
 Identities = 72/88 (81%), Positives = 74/88 (84%)
 Frame = +2 / +2

                                                                        
Query: 935  KASNPFVNLKKEYKGIFWQEDIIPFFQNVTIPKDCDTVQKCYIYLSGQVKEKLGTIDPYF 1114
            KAS PFVNLKKEYKGIFWQEDIIPFFQ  T+ KDCDTVQKCYI LS QV+EKLG IDPYF
Sbjct: 14   KASIPFVNLKKEYKGIFWQEDIIPFFQAATLTKDCDTVQKCYIALSHQVREKLGKIDPYF 193

                                        
Query: 1115 VKLGDAMVTWIEAWDELNPSTPAAANGK 1198
             KL DAMVTWIEAWD LNP   A ANGK
Sbjct: 194  AKLADAMVTWIEAWDTLNPKEGAEANGK 277




 Score =  130 bits (279), Expect = 5e-031
 Identities = 62/90 (68%), Positives = 67/90 (74%)
 Frame = -1 / -2

                                                                        
Query: 1203 LALPFAAAGVDGFSSSQASIQVTMASPSLTK*GSIVPSFSFTCPER*M*HFWTVSQSLGM 1024
            + LP A+A   G S S ASIQVTMAS SL K*GSI+PSFS T  E  M*HF TVSQSL  
Sbjct: 282  VGLPLASAPSLGLSVSHASIQVTMASASLAK*GSILPSFSLTWCESAM*HFCTVSQSLVS 103

                                          
Query: 1023 VTFWKKGMMSSCQKMPLYSFFKLTKGLLAL 934
            V  WKKGMMSSCQKMPLYSF + T G+LAL
Sbjct: 102  VAAWKKGMMSSCQKMPLYSFLRFTNGMLAL 13



  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 15,933,341
Number of Sequences: 8143
Number of extensions: 235819
Number of successful extensions: 1258
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 570499700
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)