TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= QAC22h02.sg.3.6
(2204 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819783.1|CN819783 HRO4404_A07_B14ZS5 Lib AA069E1X Aven... 333 6e-092
>gb|CN819783.1|CN819783 HRO4404_A07_B14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4404_A07_B14, mRNA
sequence
Length = 662
Score = 333 bits (722), Expect = 6e-092
Identities = 141/156 (90%), Positives = 150/156 (96%)
Frame = +1 / +3
Query: 1393 TARINGLLGNIDANTGDPQVGWDTDQFMTDIAEATLVMSSVVKNGGLAPGGFNFDAKLRR 1572
+AR NGLLGNIDANTGDPQVGWDTD+F+TDI+EATLVMSSVVKNGGLAPGGFNFDAKLRR
Sbjct: 3 SARGNGLLGNIDANTGDPQVGWDTDEFLTDISEATLVMSSVVKNGGLAPGGFNFDAKLRR 182
Query: 1573 ESTDVEDMFLAHISGMDTLARGLRNVAKLIEDGSLDDLVRKRYQSFDSEIGALIEAGKGD 1752
ESTDVEDMF+AHISGMDT+ARGL NVAKLIEDGSLD+LVRKRYQSFDSEIGA+IEAGKGD
Sbjct: 183 ESTDVEDMFIAHISGMDTMARGLHNVAKLIEDGSLDELVRKRYQSFDSEIGAMIEAGKGD 362
Query: 1753 FETLEKKVLEWGEPTVASGKQELAEILFQSAL*MMV 1860
FETLEKKVLEWGEP V SGKQELAE+LFQSAL* V
Sbjct: 363 FETLEKKVLEWGEPIVPSGKQELAEMLFQSAL*TTV 470
Score = 280 bits (606), Expect(2) = 9e-077
Identities = 125/155 (80%), Positives = 130/155 (83%)
Frame = -1 / -2
Query: 1859 TIIYRADWNRISASSCLPDATVGSPHSRTFFSSVSKSPFPASIKAPISLSKLWYRLRTRS 1680
T++YRADWN ISASSCLPD T+GSPHS+TFFSSVSKSPFPASI APISLSKLW RLRT S
Sbjct: 469 TVVYRADWNSISASSCLPDGTIGSPHSKTFFSSVSKSPFPASIMAPISLSKLW*RLRTSS 290
Query: 1679 SREPSSISLATLRRPRARVSIPEIWARNMSSTSVLSLRNLASKLKPPGASPPFLTTLDIT 1500
SREPSSISLATL RPRA VSIPEI A NMSSTSVLSLRN ASKLKPPGASPPFLTTLDIT
Sbjct: 289 SREPSSISLATLWRPRAMVSIPEI*AINMSSTSVLSLRNFASKLKPPGASPPFLTTLDIT 110
Query: 1499 KXXXXXXXXXXXXSQPT*GSPVFASIFPSSPLIRA 1395
K SQPT GSPVFAS+FPS PL RA
Sbjct: 109 KVASEISVRNSSVSQPTCGSPVFASMFPSRPLPRA 5
Score = 266 bits (576), Expect = 8e-072
Identities = 105/151 (69%), Positives = 121/151 (80%)
Frame = +3 / +2
Query: 1407 WAAWKYRCKHW*SSSWLGHRSVHDRHCRGYFGYVKCS*EWWTCTWWFQL*CQIAEGKY*C 1586
W+AWK+ KHW*S+SWLGHR V R+ RGYFGYVKC *EWW C WW QL +IAEG++*C
Sbjct: 17 WSAWKH*RKHW*STSWLGHR*VPHRYFRGYFGYVKCG*EWWACAWWLQLRREIAEGEH*C 196
Query: 1587 *GHVSCPYLRNGHPGTWPP*RCQAD*GWFPR*SCPQAVPEL***DWCLDRGWERRL*DAR 1766
* HV C YLR+GH G+WPP CQA+*GWFP *+CPQA+PEL***D C D GWERRL*D R
Sbjct: 197 *RHVYCSYLRDGHHGSWPPQCCQAN*GWFP**ACPQALPEL***DRCHD*GWERRL*DTR 376
Query: 1767 KEGSRVG*AHCCIRQTGTGGDSVPVCPIDDG 1859
KEG VG* +C I QTGTG D+VP+C +DDG
Sbjct: 377 KEGLGVG*TNCSIWQTGTG*DAVPICSVDDG 469
Score = 223 bits (482), Expect = 7e-059
Identities = 106/149 (71%), Positives = 111/149 (74%)
Frame = +2 / +1
Query: 1397 HGLMGCLEISMQTLVILKLVGTQISS*QTLQRLLWLCQV*LRMVDLHLVVSTLMPNCGGK 1576
H M CLE QTLVI KLVGTQ+SS Q QRLLWLCQV LRMV L LV ST NCGG+
Sbjct: 7 HEAMVCLETLTQTLVIHKLVGTQMSSSQIFQRLLWLCQVWLRMVGLRLVASTSTRNCGGR 186
Query: 1577 VLMLRTCFLPISPEWTPWHVASVTLPS*LRMVP*MILSASGTRALIVRLVP*SRLGKETL 1756
LML+TC L IS WTPW VAS LPS*LRMVP M LSAS TRALIVR VP* RLGKETL
Sbjct: 187 ALMLKTCLLLISQGWTPWLVASTMLPS*LRMVPLMSLSASATRALIVRSVP*LRLGKETL 366
Query: 1757 RR*KRRF*SGVSPLLHPANRNWRRFCSSL 1843
R *KRR SGV+ L H ANRNW R CS+L
Sbjct: 367 RH*KRRSWSGVNQLFHLANRNWLRCCSNL 453
Score = 197 bits (425), Expect(2) = 9e-053
Identities = 85/120 (70%), Positives = 96/120 (80%)
Frame = -3 / -1
Query: 1755 KVSFPSLDQGTNLTIKALVPLADKII*GTILNQLGNVTEATCQGVHSGDMGKKHVLNIST 1576
KVSFPSL+ GT+LTIKALV LADK+I GTILN LGN+ EAT GVH DM KHV NIS
Sbjct: 365 KVSFPSLNHGTDLTIKALVALADKLIKGTILN*LGNIVEATSHGVHP*DMSNKHVFNISA 186
Query: 1575 FPPQFGIKVETTRCKSTILNYT*HNQSSLCNVCHELICVPTNLRITSVCIDISKQPINPC 1396
PPQF ++VE TR K TILN+T*HNQSSL N+C ELICVPTNL ITSVC+++SKQ I C
Sbjct: 185 LPPQFRVEVEATRRKPTILNHT*HNQSSL*NICEELICVPTNLWITSVCVNVSKQTIASC 6
Score = 168 bits (362), Expect(2) = 9e-053
Identities = 69/99 (69%), Positives = 74/99 (74%)
Frame = -2 / -3
Query: 1858 PSSIGQTGTESPPVPVCRMQQWAHPTLEPSFLASQSLLSQPRSRHQSHYQSSGTACGQDH 1679
PSS Q GT S PVPVC+M+Q HPT PSFL SQSLLSQP+S H+SHYQSSG+ACGQ H
Sbjct: 468 PSSTEQIGTASQPVPVCQMEQLVHPTPRPSFLVSQSLLSQPQSWHRSHYQSSGSACGQAH 289
Query: 1678 LGNHPQSAWQRYGGHVPGCPFRRYGQETCPQHQYFPSAI 1562
GNHPQ AWQ GGH P CP RY Q TC QHQ PSAI
Sbjct: 288 QGNHPQLAWQHCGGHEPWCPSLRYEQ*TCLQHQCSPSAI 172
Score = 55.1 bits (114), Expect(2) = 9e-053
Identities = 24/34 (70%), Positives = 26/34 (76%)
Frame = -2 / -3
Query: 1507 T*PK*PLQCLS*TDLCPNQLEDHQCLHRYFQAAH 1406
T*PK*PL+ L T LCPNQL DHQCL + FQA H
Sbjct: 117 T*PK*PLKYL*GTHLCPNQLVDHQCLRQCFQADH 16
Score = 26.3 bits (51), Expect(2) = 9e-053
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = -3 / -1
Query: 1842 RLEQNLRQFLFAGCNS 1795
RLEQ+L QFLFA N+
Sbjct: 452 RLEQHLSQFLFARWNN 405
Score = 23.5 bits (45), Expect(2) = 9e-077
Identities = 7/8 (87%), Positives = 8/8 (100%)
Frame = -3 / -2
Query: 1887 CLFLDLWT 1864
CLFL+LWT
Sbjct: 490 CLFLELWT 467
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 27,926,203
Number of Sequences: 8143
Number of extensions: 457886
Number of successful extensions: 2005
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 46
effective length of database: 1,192,909
effective search space used: 820721392
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)