TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 8010496.2.1
(1046 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821108.1|CN821108 HRO4427_F02_L03ZS5 Lib AA069E1X Aven... 261 4e-078
gb|CN821143.1|CN821143 HRO4428_A02_B04ZS5 Lib AA069E1X Aven... 173 3e-047
gb|CN821037.1|CN821037 HRO4422_G12_M24ZS5 Lib AA069E1X Aven... 106 2e-030
gb|CN821305.1|CN821305 HRO4423_G12_N23ZS5 Lib AA069E1X Aven... 102 3e-029
gb|CN820388.1|CN820388 HRO4418_G08_M16ZS5 Lib AA069E1X Aven... 101 4e-029
>gb|CN821108.1|CN821108 HRO4427_F02_L03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_F02_L03, mRNA
sequence
Length = 714
Score = 261 bits (564), Expect(3) = 4e-078
Identities = 99/151 (65%), Positives = 120/151 (79%)
Frame = -3 / +2
Query: 747 KGGMALFNHGRGQPLQYVVANSFLAALYADYMEAVNVPGWYCGPNFMTTNDLREFARSQI 568
KGG+ NHGR QPLQYVV +FLA+LYADY+++ + PGWYCGPNF T+ LR+FA+SQI
Sbjct: 98 KGGLIQLNHGRPQPLQYVVNAAFLASLYADYLDSADTPGWYCGPNFYKTDVLRKFAKSQI 277
Query: 567 NYILGDNPKKMSYVVGFGSKYPRHVHHRGASTPHNGVKYSCTGGYKWRDSKKADPNLLGG 388
+YILG NP+KMSYVVG+G KYP+ VHHRGAS PHNGV Y C GG+KWR+SKKA+PN+L G
Sbjct: 278 DYILGKNPQKMSYVVGYGKKYPKRVHHRGASIPHNGVHYGCKGGFKWRESKKANPNILVG 457
Query: 387 AMVGGPDKNDGFKDSRNTSGRTSRRWSGNAG 295
AMV GPD++DGFKD R T + NAG
Sbjct: 458 AMVAGPDRHDGFKDIRTNYNYTEPTLAANAG 550
Score = 138 bits (296), Expect(3) = 2e-037
Identities = 71/136 (52%), Positives = 77/136 (56%)
Frame = +2 / -1
Query: 338 LRESLKPSFLSGPPTMAPPSRLGSAFLLSRHL*PPVHEYLTPLCGVDAPRWCTCRGYLLP 517
+R SLKPS LSGP T+AP G AFL S HL PP+H TPL G+DAP T GY P
Sbjct: 507 VRISLKPSCLSGPATIAPTRIFGFAFLDSLHLKPPLHP*WTPL*GIDAPL**TRLGYFFP 328
Query: 518 NPTT*LIFLGLSPRM*LI*DLANSRRSLVVMKFGPQYQPGTLTXXXXXXXXXXRKELATT 697
PTT*LIF G PRM* I D AN RR+ V+ K GPQY PG R TT
Sbjct: 327 *PTT*LIFCGFLPRM*SICDFANLRRTSVL*KLGPQYHPGVSALSR*SAYKEARNAALTT 148
Query: 698 YCSGCPLPWLKSAIPP 745
Y SG PWL PP
Sbjct: 147 Y*SG*GRPWLSCINPP 100
Score = 35.0 bits (70), Expect(3) = 4e-078
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = -2 / +2
Query: 823 LAGYHNATSLNMCMYFPGFAAFNFTXG 743
L +HN T MC Y P + +FNFT G
Sbjct: 23 LRTFHNQTDNVMCSYLPVYNSFNFTKG 103
Score = 29.9 bits (59), Expect(3) = 4e-078
Identities = 11/17 (64%), Positives = 14/17 (82%)
Frame = -2 / +2
Query: 244 TAVDKNTMFSAVPHNVP 194
T++DKNT+FSAVP P
Sbjct: 599 TSIDKNTIFSAVPPMFP 649
Score = 25.8 bits (50), Expect(3) = 2e-037
Identities = 11/17 (64%), Positives = 12/17 (70%)
Frame = +3 / -1
Query: 195 GTLCGTAENMVFLSTAV 245
G + GTAE MVFLS V
Sbjct: 648 GNIGGTAEKMVFLSIEV 598
Score = 24.9 bits (48), Expect(3) = 2e-037
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +3 / -1
Query: 750 VKLKAAKPGKYMHMLRLVALW 812
VKL GKY HM V LW
Sbjct: 96 VKLNELYTGKYEHMTLSVWLW 34
>gb|CN821143.1|CN821143 HRO4428_A02_B04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4428_A02_B04, mRNA
sequence
Length = 636
Score = 173 bits (373), Expect(2) = 3e-047
Identities = 66/99 (66%), Positives = 79/99 (79%)
Frame = -3 / +2
Query: 591 REFARSQINYILGDNPKKMSYVVGFGSKYPRHVHHRGASTPHNGVKYSCTGGYKWRDSKK 412
R+FA+SQI+YILG NP+KMSYVVG+G KYP+ VHHRGAS PHNGV Y C GG+KWR+SKK
Sbjct: 11 RKFAKSQIDYILGKNPQKMSYVVGYGKKYPKRVHHRGASIPHNGVHYGCKGGFKWRESKK 190
Query: 411 ADPNLLGGAMVGGPDKNDGFKDSRNTSGRTSRRWSGNAG 295
A+PN+L GAMV GPD++DGFKD R T + NAG
Sbjct: 191 ANPNILVGAMVAGPDRHDGFKDIRTNYNYTEPTLAANAG 307
Score = 99.1 bits (210), Expect(2) = 2e-024
Identities = 51/85 (60%), Positives = 55/85 (64%)
Frame = +2 / -1
Query: 338 LRESLKPSFLSGPPTMAPPSRLGSAFLLSRHL*PPVHEYLTPLCGVDAPRWCTCRGYLLP 517
+R SLKPS LSGP T+AP G AFL S HL PP+H TPL G+DAP T GY P
Sbjct: 264 VRISLKPSCLSGPATIAPTRIFGFAFLDSLHLKPPLHP*WTPL*GIDAPL**TRFGYFFP 85
Query: 518 NPTT*LIFLGLSPRM*LI*DLANSR 592
PTT*LIF G PRM* I D AN R
Sbjct: 84 *PTT*LIFCGFLPRM*SICDFANLR 10
Score = 30.4 bits (60), Expect(2) = 3e-047
Identities = 12/17 (70%), Positives = 14/17 (82%)
Frame = -2 / +2
Query: 244 TAVDKNTMFSAVPHNVP 194
T+VDKNT+FSAVP P
Sbjct: 356 TSVDKNTIFSAVPPMFP 406
Score = 28.6 bits (56), Expect(2) = 2e-024
Identities = 12/17 (70%), Positives = 13/17 (76%)
Frame = +3 / -1
Query: 195 GTLCGTAENMVFLSTAV 245
G + GTAE MVFLST V
Sbjct: 405 GNIGGTAEKMVFLSTEV 355
>gb|CN821037.1|CN821037 HRO4422_G12_M24ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4422_G12_M24, mRNA
sequence
Length = 784
Score = 106 bits (226), Expect(2) = 2e-030
Identities = 45/100 (45%), Positives = 58/100 (58%)
Frame = -3 / +2
Query: 768 SPPSTSPKGGMALFNHGRGQPLQYVVANSFLAALYADYMEAVNVPGWYCGPNFMTTNDLR 589
SP +T + L LQ+ +FLA +Y+DYM C + + D+R
Sbjct: 44 SPSATGSRTSGGLVWISGWNSLQHATNAAFLAVVYSDYMLTSQTAAVECSGKYFSPTDIR 223
Query: 588 EFARSQINYILGDNPKKMSYVVGFGSKYPRHVHHRGASTP 469
FA SQ NYILGDNP K+SY+VG+GS YP+ VHHRGAS P
Sbjct: 224 NFAISQANYILGDNPMKLSYLVGYGSNYPQQVHHRGASIP 343
Score = 41.4 bits (84), Expect(2) = 2e-030
Identities = 16/39 (41%), Positives = 25/39 (64%)
Frame = -3 / +2
Query: 438 GYKWRDSKKADPNLLGGAMVGGPDKNDGFKDSRNTSGRT 322
G+ + S +PN+ GA+VGGP +ND F D R+ + +T
Sbjct: 368 GFDYLHSTSPNPNIAMGALVGGPFQNDSFVDLRDNAVQT 484
>gb|CN821305.1|CN821305 HRO4423_G12_N23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4423_G12_N23, mRNA
sequence
Length = 815
Score = 102 bits (217), Expect(2) = 3e-029
Identities = 44/100 (44%), Positives = 57/100 (57%)
Frame = -3 / +1
Query: 768 SPPSTSPKGGMALFNHGRGQPLQYVVANSFLAALYADYMEAVNVPGWYCGPNFMTTNDLR 589
SP +T + L LQ+ +FLA +Y+DYM C + + +R
Sbjct: 19 SPSATGSRTSGGLVWISGWNSLQHATNAAFLAVVYSDYMLTSQTAAVECSGKYFSPTYIR 198
Query: 588 EFARSQINYILGDNPKKMSYVVGFGSKYPRHVHHRGASTP 469
FA SQ NYILGDNP K+SY+VG+GS YP+ VHHRGAS P
Sbjct: 199 NFAISQANYILGDNPMKLSYLVGYGSNYPQQVHHRGASIP 318
Score = 41.4 bits (84), Expect(2) = 3e-029
Identities = 16/39 (41%), Positives = 25/39 (64%)
Frame = -3 / +1
Query: 438 GYKWRDSKKADPNLLGGAMVGGPDKNDGFKDSRNTSGRT 322
G+ + S +PN+ GA+VGGP +ND F D R+ + +T
Sbjct: 343 GFDYLHSTSPNPNIAMGALVGGPFQNDSFVDLRDNAVQT 459
>gb|CN820388.1|CN820388 HRO4418_G08_M16ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4418_G08_M16, mRNA
sequence
Length = 679
Score = 101 bits (216), Expect(2) = 4e-029
Identities = 39/72 (54%), Positives = 49/72 (68%)
Frame = -3 / +3
Query: 684 SFLAALYADYMEAVNVPGWYCGPNFMTTNDLREFARSQINYILGDNPKKMSYVVGFGSKY 505
+FLA +Y+DYM C + + D+R FA SQ NYILGDNP K+SY+VG+GS Y
Sbjct: 36 AFLAVVYSDYMLTSQTAAVECSGKYFSPTDIRNFAISQANYILGDNPMKLSYLVGYGSNY 215
Query: 504 PRHVHHRGASTP 469
P+ VHHRGAS P
Sbjct: 216 PQQVHHRGASIP 251
Score = 41.4 bits (84), Expect(2) = 4e-029
Identities = 16/39 (41%), Positives = 25/39 (64%)
Frame = -3 / +3
Query: 438 GYKWRDSKKADPNLLGGAMVGGPDKNDGFKDSRNTSGRT 322
G+ + S +PN+ GA+VGGP +ND F D R+ + +T
Sbjct: 276 GFDYLHSTSPNPNIAMGALVGGPFQNDSFVDLRDNAVQT 392
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,440,878
Number of Sequences: 8143
Number of extensions: 103449
Number of successful extensions: 830
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 367595280
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)