TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 5704661.2.1
(798 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Aven... 338 8e-094
gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Aven... 245 6e-066
gb|CN815983.1|CN815983 HRO4515_F02_L03ZS5 Lib AA071E1X Aven... 89 5e-044
gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Aven... 159 5e-040
gb|CN814825.1|CN814825 HRO4502_H06_O12ZS5 Lib AA071E1X Aven... 88 9e-039
>gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4423_D02_H03, mRNA
sequence
Length = 733
Score = 338 bits (732), Expect = 8e-094
Identities = 120/186 (64%), Positives = 157/186 (84%)
Frame = -2 / +1
Query: 725 KLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCGESFHRYDRYLNFSNPIITKSFD 546
KLDK++FLDDDIVV+KDL GLW +++KGKVNGAVETCGESFHR+D+YLNFSNP I ++FD
Sbjct: 13 KLDKILFLDDDIVVQKDLTGLWDVDLKGKVNGAVETCGESFHRFDKYLNFSNPHIARNFD 192
Query: 545 PHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSLWKLGTLPLCLVTFWNKTFPLS 366
P+AC WA+GMN+FDL +W+ ++IT IYH WQ +NEDR LWKLGTLP L+TF+ T PL
Sbjct: 193 PNACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLD 372
Query: 365 RSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGLPKFRSYWSKYLDYDQSFLREC 186
+SWHVLGLGYNP ++ +I++AAV HYNGNMKPWLE+ + K+R YW++Y+ YD ++R C
Sbjct: 373 KSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGC 552
Query: 185 NINP*R 168
N++ *+
Sbjct: 553 NLSD*K 570
Score = 188 bits (405), Expect = 1e-048
Identities = 97/168 (57%), Positives = 104/168 (61%)
Frame = +1 / -3
Query: 220 YLDQ*LRNLGRPISNQGFMLPL*CMTAACSMSLLLTWGL*PRPRTCHDRLRGNVLFQNVT 399
YL Q R L SN GF+ PL* TAA S+S GL P PRTCHD RG V NV
Sbjct: 518 YLVQYGRYLVIASSNHGFIFPL**ATAALSISERSMLGLYPNPRTCHDLSRGCVSL*NVM 339
Query: 400 KHKGRVPSFQSDLSSLSFCHE**ISVMFCRLHSARSNTFIPKAQTQACGSKLLVIMGFEK 579
+ G VPSFQ LSS FCH *I V+ H RS FIP AQ QA GSK I GFEK
Sbjct: 338 RPGGSVPSFQRTLSSFMFCHLW*IPVISLFFHCFRSKMFIP*AQPQALGSKFRAI*GFEK 159
Query: 580 FRYRS*RWKLSPQVSTAPFTFPFMLIDQRPARSFFTTMSSSRKTILSS 723
RY S RWKLSPQVSTAP TFPF P +SF TTMSSSRK+ILS+
Sbjct: 158 LRYLSKRWKLSPQVSTAPLTFPFRSTSHNPVKSFCTTMSSSRKSILSN 15
Score = 121 bits (258), Expect = 2e-028
Identities = 64/181 (35%), Positives = 108/181 (59%)
Frame = +3 / -2
Query: 183 VAFPKERLVIIQILGPVAPKLG*TNLQPGLHVAIVMYDCSMLNVSAVNMGIVT*TKNMP* 362
VA R++I+ I PV P LG LQP LH+ IVM + S++ + AV +V+*+K+MP
Sbjct: 555 VASADIRMIILYIPCPVWPILGHCQLQPWLHIPIVMSNSSIVYLRAVYAWVVS*SKDMPR 376
Query: 363 PAEGKCLVPECDQTQRESTKFPE*SIFIKLLPRVINFSYVLPPPFSQVKHIHSKSPNTSM 542
+G C + E + + + + P+ +FI +LP V++ + P +K +H SP TS+
Sbjct: 375 LIKGMCQLVERHEARWKCPELPKNPVFIHVLPLVVDPGNIFVLPLL*IKDVHPISPTTSI 196
Query: 543 WVKTLGDNGI*KIQVSIIAMETLSTSLNSTVYLPLHVD*PKASQILLYYDVIIKKDHLVK 722
+K + I*KI++ + +ETLST L+ T+ L ++ P + QI L+ VII++ +L++
Sbjct: 195 RIKVPSNMRI*KIKILVKTVETLSTGLHCTIDLSF*INIP*SCQIFLHNYVIIEEKYLIQ 16
Query: 723 L 725
L
Sbjct: 15 L 13
>gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_A06_B12, mRNA sequence
Length = 570
Score = 245 bits (530), Expect = 6e-066
Identities = 89/143 (62%), Positives = 115/143 (80%)
Frame = -2 / +1
Query: 605 FHRYDRYLNFSNPIITKSFDPHACVWAFGMNVFDLAEWRRQNITEIYHSWQKLNEDRSLW 426
F R+D+YLNFSNP I ++FD +AC WA+GMN+FDL EW++++IT IYH WQ LNE+R LW
Sbjct: 22 FPRFDKYLNFSNPNIAQNFDANACGWAYGMNMFDLEEWKKKDITGIYHRWQTLNENRLLW 201
Query: 425 KLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGLP 246
KLGTLP L+TF+ T PL +SWHVLGLGYNP V +I+ AAVIHYNGNMKPWLEI +
Sbjct: 202 KLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPTVEHSEIDSAAVIHYNGNMKPWLEIAMT 381
Query: 245 KFRSYWSKYLDYDQSFLRECNIN 177
K+R YW+KY++Y+ S++ C I+
Sbjct: 382 KYRPYWTKYINYEHSYVHGCKIS 450
Score = 124 bits (265), Expect = 2e-029
Identities = 73/153 (47%), Positives = 82/153 (53%)
Frame = +1 / -2
Query: 142 SEGTKSTRFLHGLMLHSRRKDWS*SRYLDQ*LRNLGRPISNQGFMLPL*CMTAACSMSLL 321
S+ S ++ L+LH + S* Y Q* R IS+ GFM PL* MTAA S+S
Sbjct: 485 SQKNASLATVY*LILHPWT*ECS*LMYFVQ*GRYFVIAISSHGFMFPL*WMTAALSISEC 306
Query: 322 LTWGL*PRPRTCHDRLRGNVLFQNVTKHKGRVPSFQSDLSSLSFCHE**ISVMFCRLHSA 501
T GL P P TCHD G V V + G VPSF S L S CH *I VM HS+
Sbjct: 305 STVGLYPNPSTCHDLSSGCVSL*KVIRPGGSVPSFHSSLFSFRVCHLW*IPVMSFFFHSS 126
Query: 502 RSNTFIPKAQTQACGSKLLVIMGFEKFRYRS*R 600
RSN FIP AQ A SK I GFEK RY S R
Sbjct: 125 RSNIFIP*AQPHALASKFWAIFGFEKLRYLSNR 27
Score = 103 bits (220), Expect = 3e-023
Identities = 50/130 (38%), Positives = 82/130 (63%)
Frame = +3 / -1
Query: 204 LVIIQILGPVAPKLG*TNLQPGLHVAIVMYDCSMLNVSAVNMGIVT*TKNMP*PAEGKCL 383
++II +L PV P L +LQP HV++V+ D ++ +N +V+*+K++P* + C
Sbjct: 423 MLIIDVLCPVRPVLCHRDLQPWFHVSVVVDDSGAVDFRVLNGWVVS*SKHVP*FVQRVCQ 244
Query: 384 VPECDQTQRESTKFPE*SIFIKLLPRVINFSYVLPPPFSQVKHIHSKSPNTSMWVKTLGD 563
+ E +T + +FP+ + I+ LP ++N S VL PF Q+KHIHS SP T + +K LG+
Sbjct: 243 LVESHKTWWKCPQFPQQPVLIQGLPSMVNPSNVLLLPFFQIKHIHSISPTTCISIKVLGN 64
Query: 564 NGI*KIQVSI 593
+*KI+V +
Sbjct: 63 IWV*KIEVLV 34
>gb|CN815983.1|CN815983 HRO4515_F02_L03ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_F02_L03, mRNA sequence
Length = 668
Score = 89.0 bits (188), Expect(3) = 5e-044
Identities = 31/79 (39%), Positives = 52/79 (65%)
Frame = -2 / +1
Query: 407 LCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGLPKFRSYW 228
+ L++F + +PL + GLGY+ ++ +A +HYNGNMKPWLE+G+ +++ YW
Sbjct: 379 ISLLSFQHLIYPLDERLILSGLGYDYGIDPEVARSSASLHYNGNMKPWLELGILEYKKYW 558
Query: 227 SKYLDYDQSFLRECNINP* 171
++L + F+ ECN+NP*
Sbjct: 559 RRFLTREDRFMDECNVNP* 615
Score = 79.9 bits (168), Expect(3) = 5e-044
Identities = 29/55 (52%), Positives = 43/55 (78%)
Frame = -2 / +1
Query: 776 YLSILNHLRFYLPEMHPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCG 612
YLS+ +H F++PE+ L K+V LDDD+V+++DL+ LW+++M KVNGAVE CG
Sbjct: 22 YLSLFSHSHFFIPEIFKDLKKVVVLDDDVVIQRDLSFLWNLDMGDKVNGAVEFCG 186
Score = 42.3 bits (86), Expect(3) = 5e-044
Identities = 12/29 (41%), Positives = 20/29 (68%)
Frame = -2 / +1
Query: 551 FDPHACVWAFGMNVFDLAEWRRQNITEIY 465
+DP +C W G+NV +L +W++ +TE Y
Sbjct: 229 YDPKSCAWKSGVNVINLDKWKKHKVTEKY 315
>gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_D01_H01, mRNA sequence
Length = 653
Score = 159 bits (342), Expect = 5e-040
Identities = 57/94 (60%), Positives = 75/94 (79%)
Frame = -2 / +1
Query: 455 QKLNEDRSLWKLGTLPLCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGN 276
Q NE+R LWKLGTLP L+TF++ T PL +SWHVLGLGYNP ++ +I +AAV+H+NGN
Sbjct: 331 QTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGN 510
Query: 275 MKPWLEIGLPKFRSYWSKYLDYDQSFLRECNINP 174
MKPWL+IG+ +FR W+KY+DY SF+R+CN P
Sbjct: 511 MKPWLDIGMNQFRHLWTKYVDYGDSFIRQCNFAP 612
>gb|CN814825.1|CN814825 HRO4502_H06_O12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_H06_O12, mRNA sequence
Length = 837
Score = 87.7 bits (185), Expect(3) = 9e-039
Identities = 31/79 (39%), Positives = 51/79 (64%)
Frame = -2 / +1
Query: 407 LCLVTFWNKTFPLSRSWHVLGLGYNPHVNSRDIEHAAVIHYNGNMKPWLEIGLPKFRSYW 228
+ L++F + +PL + GLGY+ ++ +A +HYNGNMKPWLE+G+ +++ YW
Sbjct: 316 ISLLSFQHLIYPLDERLTLSGLGYDYGIDPEVARSSASLHYNGNMKPWLELGILEYKKYW 495
Query: 227 SKYLDYDQSFLRECNINP* 171
++L + F+ ECN NP*
Sbjct: 496 RRFLTREDRFMDECNANP* 552
Score = 62.0 bits (129), Expect(3) = 9e-039
Identities = 23/40 (57%), Positives = 32/40 (80%)
Frame = -2 / +1
Query: 731 HPKLDKMVFLDDDIVVKKDLAGLWSINMKGKVNGAVETCG 612
H L K+V LDDD+V+++DL+ LW+++M KVNGAVE CG
Sbjct: 4 HEDLKKVVVLDDDVVIQRDLSFLWNLDMGDKVNGAVEFCG 123
Score = 43.7 bits (89), Expect(3) = 9e-039
Identities = 13/29 (44%), Positives = 20/29 (68%)
Frame = -2 / +1
Query: 551 FDPHACVWAFGMNVFDLAEWRRQNITEIY 465
+DP +C W G+NV +L +WR+ +TE Y
Sbjct: 166 YDPKSCAWMSGVNVINLDKWRKHKVTENY 252
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,552,391
Number of Sequences: 8143
Number of extensions: 169203
Number of successful extensions: 1151
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 270249036
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)