TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 5704580.2.1
         (721 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN820882.1|CN820882  HRO4421_A06_A11ZS5 Lib AA069E1X Aven...   213   3e-056

>gb|CN820882.1|CN820882 HRO4421_A06_A11ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
           leaf Avena sativa cDNA clone HRO4421_A06_A11, mRNA
           sequence
          Length = 788

 Score =  213 bits (459), Expect = 3e-056
 Identities = 89/102 (87%), Positives = 97/102 (95%)
 Frame = -3 / +2

                                                                       
Query: 692 VEACIEETIVVYVDHLLSQKNYIKEETIERMRLDEEVLMDFFREHINVTKVESRVRILAD 513
           VE+C+EETIVVYVDHLLSQKNYIKEE +ERMRLDEEVLMDFFRE+ NVTKVE+RVR+LAD
Sbjct: 482 VESCLEETIVVYVDHLLSQKNYIKEEMVERMRLDEEVLMDFFREYTNVTKVENRVRVLAD 661

                                                     
Query: 512 MRELASAESLDSFTLIYTNILEHQPDCPPEVFYKLVAMREGI 387
           +RELASAESLDSFTLIYTNILEHQPDCP EV  KLVA+REGI
Sbjct: 662 LRELASAESLDSFTLIYTNILEHQPDCPSEVVEKLVALREGI 787




 Score =  166 bits (358), Expect = 3e-042
 Identities = 68/102 (66%), Positives = 80/102 (78%)
 Frame = +3 / -2

                                                                       
Query: 387 NAFPHCHKLIKNLRRAIRLMFQYISVNESKAIQTLS*S*LPHICQNPHSAFNFCHVDVFP 566
           N+FP C+KL+ NLRRAIRL+FQ I +NES+ IQT S*S  P I QNP+S  NFCH+ VF 
Sbjct: 787 NSFPQCNKLLNNLRRAIRLVFQNICINESETIQTFS*SKFP*ISQNPYSVLNFCHISVFS 608

                                                     
Query: 567 EEIHKHLFI*PHPLYRFFFYVVFLRKEMVNIDHNCFLYASLH 692
           EEIHKH FI*PHPL  FFFYVVFL ++M+NI + CFL A LH
Sbjct: 607 EEIHKHFFI*PHPLNHFFFYVVFLTEKMINIYNYCFL*ARLH 482




 Score =  111 bits (237), Expect(2) = 7e-031
 Identities = 49/73 (67%), Positives = 54/73 (73%)
 Frame = -1 / +3

                                                                       
Query: 604 G*G*MKRCLWISSGNTST*QKLKAECGFWQI*GS*LQLRVWIALLSFTLIYWNINLIARL 425
           G*G*MK+CLWISS NT  *QKL+ E GFW I*G+ LQL+VWI  LSF  I+WN N IARL
Sbjct: 570 G*G*MKKCLWISSENTLM*QKLRTEYGFWLI*GNLLQLKVWIVSLSFIQIFWNTNRIARL 749

                        
Query: 424 RFFISLWQCGKAF 386
           R   SL  CGK F
Sbjct: 750 RLLRSLLHCGKEF 788




 Score = 37.3 bits (75), Expect(2) = 7e-031
 Identities = 16/24 (66%), Positives = 17/24 (70%)
 Frame = -2 / +1

                                   
Query: 693 CGGLHRGNNCGLC*PSPFSEKLHK 622
           CG L RGNN  +C* S  SEKLHK
Sbjct: 481 CGVLPRGNNSCIC*SSSQSEKLHK 552



  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 8,470,211
Number of Sequences: 8143
Number of extensions: 121218
Number of successful extensions: 1191
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 238598248
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)