TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4695478.2.1
(1201 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818757.1|CN818757 HRO4468_C07_E14ZS5 Lib AA070E1X Aven... 111 1e-056
gb|CN820523.1|CN820523 HRO4413_D02_G03ZS5 Lib AA069E1X Aven... 174 2e-054
gb|CN819392.1|CN819392 HRO4405_E01_I01ZS5 Lib AA069E1X Aven... 140 9e-041
gb|CN819665.1|CN819665 HRO4402_G05_M10ZS5 Lib AA069E1X Aven... 125 2e-036
gb|CN816401.1|CN816401 HRO4520_C12_F24ZS5 Lib AA071E1X Aven... 66 1e-021
>gb|CN818757.1|CN818757 HRO4468_C07_E14ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4468_C07_E14, mRNA sequence
Length = 805
Score = 111 bits (237), Expect(4) = 1e-056
Identities = 40/62 (64%), Positives = 49/62 (79%)
Frame = -2 / +3
Query: 681 DWPKDKIKDENCWSDKEFCRNEENWYSVAKISSEEAALEYAKQTGLDVVTVNPAVVFGPL 502
DWP DKIKDE+CWSDKE C+ ENWYS+AK ++EE ALEY + GL + T+ P +VFGPL
Sbjct: 129 DWPPDKIKDESCWSDKELCKENENWYSLAKTTAEEMALEYGLKNGLHIATLLPGLVFGPL 308
Query: 501 LQ 496
LQ
Sbjct: 309 LQ 314
Score = 86.8 bits (183), Expect(4) = 1e-056
Identities = 35/71 (49%), Positives = 50/71 (70%)
Frame = -2 / +3
Query: 489 LNTSCQFLVYFLKGGPDRMRDKLWHIVDVRDTADALLLVYETPQASGRHICAPHFISARD 310
+NT+ L+Y LKGGPD M +K + +VDVRD A+ALLL+Y +S R+IC+ + +D
Sbjct: 324 VNTTSNVLIYILKGGPDTMNNKFYPMVDVRDVAEALLLLYNKAGSSERYICSLDQMDLKD 503
Query: 309 LLELLKTMYPD 277
LL +LK MYP+
Sbjct: 504 LLGILKNMYPN 536
Score = 79.9 bits (168), Expect(4) = 2e-027
Identities = 39/62 (62%), Positives = 40/62 (64%)
Frame = +2 / -1
Query: 497 CRRGPKTTAGLTVTTSSPVCFAYSSAASSDEILATEYQFSSFLQNSLSDQQFSSLILSLG 676
C RGPKT G V SP YS A SS +LA EYQFS L NSLSDQQ SSLILS G
Sbjct: 313 CNRGPKTRPGNNVAMCSPFFSPYSRAISSAVVLAREYQFSFSLHNSLSDQQLSSLILSGG 134
Query: 677 QS 682
QS
Sbjct: 133 QS 128
Score = 48.7 bits (100), Expect(4) = 1e-056
Identities = 18/52 (34%), Positives = 33/52 (63%)
Frame = -2 / +3
Query: 255 ESIYDMEHPAPMTSDKLKKLGWKVRPLKEPIAETVEFCQHAGFFEDVEGFRF 100
+ + D+++ +TSDKLK LGWK R L+E + ++++ + AG + +G F
Sbjct: 549 DKMVDVDYKVIVTSDKLKNLGWKPRKLEETLEDSIKSYEKAGLLQSSDGKPF 704
Score = 38.2 bits (77), Expect(4) = 2e-027
Identities = 22/53 (41%), Positives = 28/53 (52%)
Frame = +2 / -1
Query: 341 MCLPEACGVSYTSRRASAVSLTSTMCQSLSLIRSGPPFRK*TRN*QLVLSVGC 499
M L + + Y ASA S TST+ +L I SGPPF * R +VL+ C
Sbjct: 472 MYLSDDPALLYNRSNASATSRTSTIG*NLLFIVSGPPFSM*MRTLLVVLTATC 314
Score = 28.1 bits (55), Expect(4) = 2e-027
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = +2 / -1
Query: 110 PSTSSKNPACWQNSTVSAMGSFSGLTFHPSFLSLSDV 220
PS + PA + +S+ S + L FHP F SLS+V
Sbjct: 694 PSEL*RRPAFS*DLMLSSSVSSNFLGFHPKFFSLSEV 584
Score = 26.3 bits (51), Expect(4) = 2e-027
Identities = 11/14 (78%), Positives = 11/14 (78%)
Frame = +2 / -1
Query: 755 LSAFMVPVTAGPSI 796
LS FMVP TAG SI
Sbjct: 58 LSTFMVPFTAGSSI 17
Score = 25.4 bits (49), Expect(4) = 1e-056
Identities = 9/16 (56%), Positives = 12/16 (75%)
Frame = -1 / +2
Query: 799 RDAGSCCYRHHKRTQG 752
++AGSC HH+ TQG
Sbjct: 14 KNAGSCREGHHECTQG 61
>gb|CN820523.1|CN820523 HRO4413_D02_G03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4413_D02_G03, mRNA
sequence
Length = 704
Score = 174 bits (375), Expect(3) = 2e-054
Identities = 67/137 (48%), Positives = 98/137 (71%)
Frame = -2 / +3
Query: 690 NPKDWPKDKIKDENCWSDKEFCRNEENWYSVAKISSEEAALEYAKQTGLDVVTVNPAVVF 511
N +WPK K+ DE+ WSD++ CR ++WY ++K +E A YA +TGLD+VT+ P++V
Sbjct: 138 NNPNWPKGKVLDEDNWSDEDLCRKNKDWYDLSKTLAEREAFAYAAKTGLDIVTICPSLVI 317
Query: 510 GPLLQPTLNTSCQFLVYFLKGGPDRMRDKLWHIVDVRDTADALLLVYETPQASGRHICAP 331
GPLLQ T+NTS + L+ + KG + + +K ++VDVRD ADA+LL YE +ASGR+IC+
Sbjct: 318 GPLLQSTVNTSSKVLLSYFKGEHETVENKYRNLVDVRDVADAILLAYENAEASGRYICSS 497
Query: 330 HFISARDLLELLKTMYP 280
I A DL+ +LKT+YP
Sbjct: 498 SPIKASDLISILKTLYP 548
Score = 43.2 bits (88), Expect(3) = 2e-054
Identities = 15/38 (39%), Positives = 27/38 (71%)
Frame = -2 / +3
Query: 243 DMEHPAPMTSDKLKKLGWKVRPLKEPIAETVEFCQHAG 130
++EH +S+KL+KLGW RP++E + ++VE + +G
Sbjct: 576 EVEHSTTYSSEKLQKLGWTFRPIEETLRDSVESYRASG 689
Score = 29.0 bits (57), Expect(3) = 2e-054
Identities = 11/23 (47%), Positives = 15/23 (65%)
Frame = -2 / +3
Query: 822 GKITDPEREMLGPAVTGTINALK 754
G + P ++ PAVTGT+N LK
Sbjct: 9 GALLQP*GRIIAPAVTGTLNELK 77
>gb|CN819392.1|CN819392 HRO4405_E01_I01ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4405_E01_I01, mRNA
sequence
Length = 717
Score = 140 bits (301), Expect(2) = 9e-041
Identities = 61/180 (33%), Positives = 95/180 (52%)
Frame = -2 / +1
Query: 816 ITDPEREMLGPAVTGTINALKXXXXXXXXXXXXXXXXXXVEINPKDWPKDKIKDENCWSD 637
+ DP+ E+L PAV GT+N L+ V N K + + DE +S
Sbjct: 127 VKDPKAELLDPAVNGTLNVLRSCKKASIKRVIVTSSMAAVGYNGKPKTPNVVVDETWFSH 306
Query: 636 KEFCRNEENWYSVAKISSEEAALEYAKQTGLDVVTVNPAVVFGPLLQPTLNTSCQFLVYF 457
E C + WY ++K +EEAA +++K ++VT+NPA+V GPLLQPTLNTS + ++
Sbjct: 307 AELCEKGQQWYVLSKTLAEEAAWKFSKDNAFEIVTINPAMVIGPLLQPTLNTSAEAILKL 486
Query: 456 LKGGPDRMRDKLWHIVDVRDTADALLLVYETPQASGRHICAPHFISARDLLELLKTMYPD 277
+ G + + ++V+D A A +L YE P A+GR+ +L+ + MYPD
Sbjct: 487 INGSSSTYPNFSFGWINVKDVALAHILAYEVPSANGRYCMVERVAHHSELVNFIHKMYPD 666
Score = 41.8 bits (85), Expect(2) = 9e-041
Identities = 16/37 (43%), Positives = 24/37 (64%)
Frame = -2 / +1
Query: 942 LENASENLKLFKADVLDYDAMAGAVAGCQGVFHVATP 832
L+ A + L LFKA +L+ + A+ GC+ VFH A+P
Sbjct: 4 LDGAKDRLHLFKASLLEEGSFDDAIDGCECVFHTASP 114
>gb|CN819665.1|CN819665 HRO4402_G05_M10ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4402_G05_M10, mRNA
sequence
Length = 632
Score = 125 bits (267), Expect(2) = 2e-036
Identities = 51/98 (52%), Positives = 72/98 (73%)
Frame = -2 / +2
Query: 573 ALEYAKQTGLDVVTVNPAVVFGPLLQPTLNTSCQFLVYFLKGGPDRMRDKLWHIVDVRDT 394
A YA +TGLD+VT+ P++V GPLLQ T+NTS + L+ + KG + + +K ++VDVRD
Sbjct: 11 AFAYAAKTGLDIVTICPSLVIGPLLQSTVNTSSKVLLSYFKGEHETVENKYRNLVDVRDV 190
Query: 393 ADALLLVYETPQASGRHICAPHFISARDLLELLKTMYP 280
ADA+LL YE +ASGR+IC+ I A DL+ +LKT+YP
Sbjct: 191 ADAILLAYENAEASGRYICSSSPIKASDLISILKTLYP 304
Score = 43.2 bits (88), Expect(2) = 2e-036
Identities = 15/38 (39%), Positives = 27/38 (71%)
Frame = -2 / +2
Query: 243 DMEHPAPMTSDKLKKLGWKVRPLKEPIAETVEFCQHAG 130
++EH +S+KL+KLGW RP++E + ++VE + +G
Sbjct: 332 EVEHSTTYSSEKLQKLGWTFRPIEETLRDSVESYRASG 445
>gb|CN816401.1|CN816401 HRO4520_C12_F24ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_C12_F24, mRNA sequence
Length = 735
Score = 66.1 bits (138), Expect(3) = 1e-021
Identities = 24/46 (52%), Positives = 34/46 (73%)
Frame = -2 / +1
Query: 579 EAALEYAKQTGLDVVTVNPAVVFGPLLQPTLNTSCQFLVYFLKGGP 442
EAA ++++ G+D+VT+NPA+V GPLL PTLNTS + Y + G P
Sbjct: 7 EAAWKFSEDNGIDLVTINPAMVLGPLLHPTLNTSAGMIKYLISGSP 144
Score = 39.1 bits (79), Expect(3) = 1e-021
Identities = 16/44 (36%), Positives = 27/44 (61%)
Frame = -2 / +1
Query: 411 VDVRDTADALLLVYETPQASGRHICAPHFISARDLLELLKTMYP 280
V+V+D A A +L E P ASGR+ A + ++++++ MYP
Sbjct: 172 VNVKDVALAHVLACEVPSASGRYCMADRVLHLSEVVKIIHEMYP 303
Score = 30.9 bits (61), Expect(3) = 1e-021
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = -2 / +1
Query: 222 MTSDKLKKLGWKVRPLKEPIAETVEFCQHAGF 127
++ DK+K LG ++ P + + ET+E + GF
Sbjct: 361 VSRDKIKSLGIELTPFEATLRETIESLKEKGF 456
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,357,784
Number of Sequences: 8143
Number of extensions: 193869
Number of successful extensions: 1351
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 429264225
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)