TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 4534747.2.1
(628 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF033096.1|AF033096 Avena sativa nonphototropic hypocoty... 103 5e-039
gb|AF033097.1|AF033097 Avena sativa nonphototropic hypocoty... 103 5e-039
gb|CN819189.1|CN819189 HRO4451_B10_C19ZS5 Lib AA070E1X Aven... 104 7e-039
gb|CN820364.1|CN820364 HRO4418_E06_I12ZS5 Lib AA069E1X Aven... 104 7e-039
gb|CN820184.1|CN820184 HRO4412_B04_D08ZS5 Lib AA069E1X Aven... 103 9e-035
>gb|AF033096.1|AF033096 Avena sativa nonphototropic hypocotyl 1 (NPH1-1) mRNA, complete cds
Length = 3467
Score = 103 bits (220), Expect(2) = 5e-039
Identities = 37/64 (57%), Positives = 48/64 (75%)
Frame = +2 / +2
Query: 71 STSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQP 250
S SFVGT EY+APEI+ G GH SAVDWW GI LYE+L+G TPF+G + + T N++ +
Sbjct: 2735 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 2914
Query: 251 LRFP 262
+RFP
Sbjct: 2915 IRFP 2926
Score = 72.1 bits (151), Expect(2) = 5e-039
Identities = 29/57 (50%), Positives = 37/57 (64%)
Frame = +2 / +2
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDP 448
+ VS AR LI LL +DP R+ + G+ EIK+HPFF G+NWALVR PP + P
Sbjct: 2933 ISVSLPARQLIYRLLHRDPSNRLGSYEGSNEIKEHPFFRGINWALVRGTAPPKLDAP 3103
>gb|AF033097.1|AF033097 Avena sativa nonphototropic hypocotyl 1 (NPH1-2) mRNA, complete cds
Length = 3422
Score = 103 bits (220), Expect(2) = 5e-039
Identities = 37/64 (57%), Positives = 48/64 (75%)
Frame = +2 / +2
Query: 71 STSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQP 250
S SFVGT EY+APEI+ G GH SAVDWW GI LYE+L+G TPF+G + + T N++ +
Sbjct: 2672 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 2851
Query: 251 LRFP 262
+RFP
Sbjct: 2852 IRFP 2863
Score = 72.1 bits (151), Expect(2) = 5e-039
Identities = 29/57 (50%), Positives = 37/57 (64%)
Frame = +2 / +2
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDP 448
+ VS AR LI LL +DP R+ + G+ EIK+HPFF G+NWALVR PP + P
Sbjct: 2870 ISVSLPARQLIYRLLHRDPSNRLGSYEGSNEIKEHPFFRGINWALVRGTAPPKLDAP 3040
>gb|CN819189.1|CN819189 HRO4451_B10_C19ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4451_B10_C19, mRNA sequence
Length = 791
Score = 104 bits (221), Expect(2) = 7e-039
Identities = 36/68 (52%), Positives = 49/68 (72%)
Frame = +2 / +3
Query: 71 STSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQP 250
S SFVGT EY+APE++ G GH SA+DWW GI LYE+L+G TPF+G + + T N++ +
Sbjct: 30 SNSFVGTEEYIAPEVITGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRKKTFYNILHKD 209
Query: 251 LRFPDATP 274
L FP + P
Sbjct: 210 LTFPSSIP 233
Score = 71.2 bits (149), Expect(2) = 7e-039
Identities = 27/57 (47%), Positives = 37/57 (64%)
Frame = +2 / +3
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDP 448
+PVS A+ LI GLL +DP RI + GA +IK+HPFF+ + W L+R PP + P
Sbjct: 228 IPVSLEAKQLIHGLLQRDPSARIGSNTGANDIKEHPFFEDIYWPLIRCMSPPELDVP 398
>gb|CN820364.1|CN820364 HRO4418_E06_I12ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4418_E06_I12, mRNA
sequence
Length = 699
Score = 104 bits (221), Expect(2) = 7e-039
Identities = 36/68 (52%), Positives = 49/68 (72%)
Frame = +2 / +3
Query: 71 STSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQP 250
S SFVGT EY+APE++ G GH SA+DWW GI LYE+L+G TPF+G + + T N++ +
Sbjct: 39 SNSFVGTEEYIAPEVITGAGHTSAIDWWALGILLYEMLYGRTPFRGKNRKKTFYNILHKD 218
Query: 251 LRFPDATP 274
L FP + P
Sbjct: 219 LTFPSSIP 242
Score = 71.2 bits (149), Expect(2) = 7e-039
Identities = 27/57 (47%), Positives = 37/57 (64%)
Frame = +2 / +3
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNWALVRDAHPPSVPDP 448
+PVS A+ LI GLL +DP RI + GA +IK+HPFF+ + W L+R PP + P
Sbjct: 237 IPVSLEAKQLIHGLLQRDPSARIGSNTGANDIKEHPFFEDIYWPLIRCMSPPELDVP 407
>gb|CN820184.1|CN820184 HRO4412_B04_D08ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4412_B04_D08, mRNA
sequence
Length = 610
Score = 103 bits (220), Expect(2) = 9e-035
Identities = 37/64 (57%), Positives = 48/64 (75%)
Frame = +2 / +2
Query: 71 STSFVGTHEYLAPEIVRGEGHGSAVDWWTFGIFLYELLHGTTPFKGDSNRATLCNVVDQP 250
S SFVGT EY+APEI+ G GH SAVDWW GI LYE+L+G TPF+G + + T N++ +
Sbjct: 176 SNSFVGTEEYIAPEIITGAGHTSAVDWWALGILLYEMLYGYTPFRGKTRQRTFANILHKD 355
Query: 251 LRFP 262
+RFP
Sbjct: 356 IRFP 367
Score = 57.9 bits (120), Expect(2) = 9e-035
Identities = 22/43 (51%), Positives = 29/43 (67%)
Frame = +2 / +2
Query: 278 VPVSTVARDLIRGLLVKDPQKRIATRRGAAEIKQHPFFDGVNW 406
+ VS AR LI LL +DP R+ + G+ EIK+HPFF G+NW
Sbjct: 374 ISVSLPARQLIYRLLHRDPSNRLGSHEGSNEIKEHPFFRGINW 502
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,494,731
Number of Sequences: 8143
Number of extensions: 91070
Number of successful extensions: 1411
Number of sequences better than 1.0e-020: 6
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 203429846
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)