TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3923331.2.1
(496 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818044.1|CN818044 HRO4472_D08_G16ZS5 Lib AA070E1X Aven... 194 9e-051
gb|CN817274.1|CN817274 HRO4458_D09_H18ZS5 Lib AA070E1X Aven... 124 4e-039
>gb|CN818044.1|CN818044 HRO4472_D08_G16ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4472_D08_G16, mRNA sequence
Length = 842
Score = 194 bits (418), Expect = 9e-051
Identities = 78/100 (78%), Positives = 91/100 (91%)
Frame = -3 / +1
Query: 491 RERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIY 312
RERGRFK+ESIY+RS +LTL ALE+AV FRS+NHVP+WKDERP SIRGGD+LK+YRIY
Sbjct: 364 RERGRFKTESIYLRSNKLTLQALETAVHATFRSINHVPIWKDERPSSIRGGDELKVYRIY 543
Query: 311 PVGLTQRQALYGFRFRDDSELEQYIKDHPCAKLEVIFV*L 192
PVGLT+R+ALY F+F DD+EL +YIKDHPCAKLEVIFV*L
Sbjct: 544 PVGLTEREALYKFKFSDDAELARYIKDHPCAKLEVIFV*L 663
Score = 116 bits (247), Expect = 3e-027
Identities = 58/102 (56%), Positives = 62/102 (60%)
Frame = +1 / -1
Query: 187 HSSYTKITSSFAQGWSLIYCXXXXXXLNLKPYKA*RCVRPTG*ILYNFRXXXXXXXXXXX 366
+ SYTKITSSFA G SL+Y LNL Y A R VRPTG*ILY F
Sbjct: 668 YRSYTKITSSFAHG*SLMYRASSASSLNLNLYNASRSVRPTG*ILYTFNSSPPRMLDGLS 489
Query: 367 XFHTGTWLRDLNLPSTADSKAPRVNWPDLM*MLSDLNRPLSL 492
FH GTWL DLN+ TA SKA +VN DL *MLS LN PLSL
Sbjct: 488 SFHIGTWLMDLNVACTAVSKACKVNLFDLR*MLSVLNLPLSL 363
Score = 110 bits (235), Expect = 2e-025
Identities = 56/100 (56%), Positives = 69/100 (69%)
Frame = -1 / +2
Query: 490 EREGGLSQRAFT*DQAS*LWEPWNPQCSVNLGPSTTFLYGRMKGPRALEVGMT*NYTEFT 311
EREGGL Q+AF *DQ +*L++ W +L PST FLYGRMKG +A EVGM+* TEF
Sbjct: 365 EREGGLRQKAFI*DQTN*LYKLWRLLYMPHLDPSTMFLYGRMKGHQAYEVGMS*RCTEFI 544
Query: 310 QSV*RNVRLCTVLDLGMILNSSNISKTIPVQSLK*FLCNY 191
Q V +N + T L+L M+LN + K I VQSL+*FL NY
Sbjct: 545 QLVLQNEKHYTNLNLVMMLNLLDTLKIIHVQSLR*FLYNY 664
Score = 108 bits (231), Expect = 6e-025
Identities = 53/100 (53%), Positives = 66/100 (66%)
Frame = +3 / -3
Query: 192 *LHKNYFKLCTGMVFDILLEFRIIPKSKTVQSLTLR*TDWVNSV*FQVIPTSNARGPFIL 371
*L+KNY KLCT M+F++ +F II K K V + T+W+NSV Q+IPTS A PFIL
Sbjct: 663 *LYKNYLKLCTWMIFNVSSKFSIITKFKFV*CFSFCKTNWINSVHLQLIPTSYA*WPFIL 484
Query: 372 PYRNVVEGPKFTEHCGFQGSQS*LA*SHVNAL*LKPPSLS 491
PYRN+V+G K + Q S* *S +NA LKPPSLS
Sbjct: 483 PYRNMVDGSKCGMYSSLQSL*S*FV*S*INAFCLKPPSLS 364
Score = 96.8 bits (205), Expect = 2e-021
Identities = 53/101 (52%), Positives = 67/101 (66%)
Frame = -2 / +3
Query: 489 RERAV*VREHLHEIRPVNSGSLGIRSAR*I*VPQPRSCMEG*KAPEH*RWG*PEIIQNLP 310
RER V* R+HL +I+ +N S G I*+ QP S MEG*KA +H RWG* E +QNL
Sbjct: 366 REREV*DRKHLSKIKQINFTSFGDCCTCHI*IHQPCSYMEG*KAIKHTRWG*VEGVQNLS 545
Query: 309 SRSNATSGFVRF*I*G*F*TRAIYQRPSLCKA*SNFCVTTM 187
S S T ++ *I* * *T +I+ R S+CKA* NFC+TT+
Sbjct: 546 SWSYRTRSIIQI*I***C*TCSIH*RSSMCKA*GNFCITTI 668
>gb|CN817274.1|CN817274 HRO4458_D09_H18ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4458_D09_H18, mRNA sequence
Length = 542
Score = 124 bits (266), Expect(3) = 4e-039
Identities = 49/70 (70%), Positives = 61/70 (87%)
Frame = -3 / +3
Query: 491 RERGRFKSESIYMRSGQLTLGALESAVLGKFRSLNHVPVWKDERPPSIRGGDDLKLYRIY 312
RERGR K+ESIY+RS +LTL ALESA+ FRS+NHVPVWKDERP SIRGGD+LK+Y+IY
Sbjct: 192 RERGRLKTESIYLRSNELTLEALESAIHATFRSINHVPVWKDERPSSIRGGDELKVYKIY 371
Query: 311 PVGLTQRQAL 282
PVGL + +++
Sbjct: 372 PVGLYRTRSI 401
Score = 42.3 bits (86), Expect(3) = 4e-039
Identities = 16/20 (80%), Positives = 17/20 (85%)
Frame = -3 / +1
Query: 260 DSELEQYIKDHPCAKLEVIF 201
D EL +YIKDHPCAKLEV F
Sbjct: 421 DDELARYIKDHPCAKLEVNF 480
Score = 26.7 bits (52), Expect(3) = 4e-039
Identities = 9/14 (64%), Positives = 12/14 (85%)
Frame = -3 / +1
Query: 299 TQRQALYGFRFRDD 258
T+R+ALY F+F DD
Sbjct: 385 TEREALYKFKFSDD 426
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,725,971
Number of Sequences: 8143
Number of extensions: 74940
Number of successful extensions: 1555
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 41
effective length of database: 1,233,624
effective search space used: 151735752
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)