TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3838122.2.1
(1865 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816489.1|CN816489 HRO4521_C12_E23ZS5 Lib AA071E1X Aven... 99 3e-040
gb|CN821442.1|CN821442 HRO4425_D03_G05ZS5 Lib AA069E1X Aven... 147 5e-036
gb|CN820016.1|CN820016 HRO4410_B02_C04ZS5 Lib AA069E1X Aven... 145 2e-035
emb|X73601.1|PSBFRTSID A.sativa mRNA for invertase (beta-fr... 90 1e-022
>gb|CN816489.1|CN816489 HRO4521_C12_E23ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4521_C12_E23, mRNA sequence
Length = 874
Score = 98.7 bits (209), Expect(4) = 3e-040
Identities = 37/86 (43%), Positives = 54/86 (62%)
Frame = -1 / +1
Query: 1265 DRHDYYALGRYDAAANAWTPIDAGRDVGIGLRYDWGKFYASKTFYDPAKRRRVLWGWVGE 1086
D+ D Y +G YD +A+ P D + L+ D+G +YASK+F+D RR++WGW E
Sbjct: 43 DQCDKYMIGVYDLKRDAFLPDTVLDDRRLWLKIDYGNYYASKSFFDSKNSRRIIWGWTNE 222
Query: 1085 TDSERADVSKGWASLQGIPRTVLLDT 1008
+DS DV+KGWA + IPR + LD+
Sbjct: 223 SDSSSDDVAKGWAGIYAIPRAIWLDS 300
Score = 53.3 bits (110), Expect(4) = 3e-040
Identities = 20/48 (41%), Positives = 32/48 (66%)
Frame = -1 / +1
Query: 992 LLQWPVEEVETLRTNSTDLSGITIDYGSVFPLNLRRATQLDIEAEFQL 849
LLQWPV+E+E+LR N + G+ ++ G +F + TQ D+E +F+L
Sbjct: 313 LLQWPVDEIESLRGNEINHQGLELNKGDLFEIKGIDTTQADVEIDFEL 456
Score = 46.4 bits (95), Expect(4) = 3e-040
Identities = 20/35 (57%), Positives = 23/35 (65%)
Frame = -1 / +1
Query: 581 LEDEATLSLRVLVDHSIVESFAQGGRSTATSRVYP 477
LE E +SLR L+D S VESF GGR +RVYP
Sbjct: 730 LEKERKISLRTLIDRSAVESFGGGGRLCIIARVYP 834
Score = 27.6 bits (54), Expect(4) = 3e-040
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = -1 / +1
Query: 785 GAAGRGALGPFGLFVLA 735
GA+ G +GPFGL +LA
Sbjct: 529 GASVHGGVGPFGLVILA 579
>gb|CN821442.1|CN821442 HRO4425_D03_G05ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4425_D03_G05, mRNA
sequence
Length = 502
Score = 147 bits (316), Expect = 5e-036
Identities = 67/93 (72%), Positives = 73/93 (78%)
Frame = -1 / +1
Query: 632 SSATDIVKRVVGSAVPVLEDEATLSLRVLVDHSIVESFAQGGRSTATSRVYPTEAIYANA 453
SS + + + V V + D LS+RVLVDHSIVESFAQGGRST TSRVYPTEAIYANA
Sbjct: 37 SSRANDIVKRVVGNVVPVLDGEALSVRVLVDHSIVESFAQGGRSTVTSRVYPTEAIYANA 216
Query: 452 GVFLFNNATAARVTATKLVVHEMDSSYNHDYMA 354
GV+LFNNAT ARVTAT L VHEMDSSYN YMA
Sbjct: 217 GVYLFNNATGARVTATSLTVHEMDSSYNQAYMA 315
Score = 115 bits (245), Expect(2) = 3e-032
Identities = 51/62 (82%), Positives = 54/62 (87%)
Frame = +1 / -3
Query: 355 AM*SWLYDESISWTTSFVAVTRAAVALLKRKTPALA*MASVG*TRDVAVDLPPCAKLSTM 534
AM*+WL +ESISWT VAVTRA VALLKR TPALA*MASVG TRDV VDLPPCAKLST+
Sbjct: 314 AM*AWL*EESISWTVRLVAVTRAPVALLKRYTPALA*MASVGYTRDVTVDLPPCAKLSTI 135
Query: 535 EW 540
EW
Sbjct: 134 EW 129
Score = 39.6 bits (80), Expect(2) = 3e-032
Identities = 16/24 (66%), Positives = 20/24 (83%)
Frame = +1 / -3
Query: 595 LPTTRLTMSVALEERDSSWQKCVA 666
LPTT LTMS+A ++RDSS QKC +
Sbjct: 77 LPTTLLTMSLARDDRDSSRQKCAS 6
>gb|CN820016.1|CN820016 HRO4410_B02_C04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4410_B02_C04, mRNA
sequence
Length = 525
Score = 145 bits (311), Expect = 2e-035
Identities = 65/93 (69%), Positives = 73/93 (78%)
Frame = -1 / +2
Query: 632 SSATDIVKRVVGSAVPVLEDEATLSLRVLVDHSIVESFAQGGRSTATSRVYPTEAIYANA 453
SS + + + + V + D LS+RVLVDHSIVESF QGGRST TSRVYPTEAIYANA
Sbjct: 23 SSRANDIVKRIVGNVVPVLDGEALSVRVLVDHSIVESFVQGGRSTVTSRVYPTEAIYANA 202
Query: 452 GVFLFNNATAARVTATKLVVHEMDSSYNHDYMA 354
GV+LFNNAT ARVTAT LVVHEMDSSYN Y+A
Sbjct: 203 GVYLFNNATGARVTATSLVVHEMDSSYNQAYVA 301
Score = 111 bits (236), Expect(2) = 5e-027
Identities = 49/62 (79%), Positives = 53/62 (85%)
Frame = +1 / -1
Query: 355 AM*SWLYDESISWTTSFVAVTRAAVALLKRKTPALA*MASVG*TRDVAVDLPPCAKLSTM 534
A *+WL +ESISWTT VAVTRA VALLKR TPALA*+ASVG TRDV VDLPPC KLST+
Sbjct: 300 AT*AWL*EESISWTTRLVAVTRAPVALLKRYTPALA*IASVGYTRDVTVDLPPCTKLSTI 121
Query: 535 EW 540
EW
Sbjct: 120 EW 115
Score = 26.3 bits (51), Expect(2) = 5e-027
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +1 / -1
Query: 595 LPTTRLTMSVALEERDS 645
LPT LTMS+A E+ DS
Sbjct: 63 LPTILLTMSLAREDHDS 13
>emb|X73601.1|PSBFRTSID A.sativa mRNA for invertase (beta-fructofuranosidase) (partial)
Length = 511
Score = 90.0 bits (190), Expect(2) = 1e-022
Identities = 39/85 (45%), Positives = 51/85 (60%)
Frame = -1 / +2
Query: 1862 VIKGHAVSRDLIRWRRLPLAMVPDQWYDTNGVWTGSATTLPDGRLAMLYRGSTNASVQVQ 1683
++ GHAVS DL+ W L A+ D D NG WTGSAT LP G+ ++Y G + QVQ
Sbjct: 77 IVWGHAVSTDLVNWIILEPAIERDSPGDINGCWTGSATILPGGQPIVIYTGGDAENHQVQ 256
Query: 1682 CLAVPADDADPLLTNWTKYEGNPVL 1608
+ +P + +DP L WTK NPVL
Sbjct: 257 NIMLPKNRSDPYLREWTKAGNNPVL 331
Score = 32.2 bits (64), Expect(2) = 1e-022
Identities = 11/18 (61%), Positives = 11/18 (61%)
Frame = -1 / +2
Query: 1598 PGIGPKDFRDPTTVWIDP 1545
PG FRDPTT WI P
Sbjct: 344 PGFNSSQFRDPTTGWIGP 397
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 15,806,542
Number of Sequences: 8143
Number of extensions: 227577
Number of successful extensions: 2092
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 691805952
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)