TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3712941.2.1
(1473 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN820072.1|CN820072 HRO4410_G07_M14ZS5 Lib AA069E1X Aven... 408 e-114
gb|CN818277.1|CN818277 HRO4470_C12_F24ZS5 Lib AA070E1X Aven... 295 8e-084
gb|CN816332.1|CN816332 HRO4519_E08_J15ZS5 Lib AA071E1X Aven... 150 4e-037
gb|CN820263.1|CN820263 HRO4417_D05_G09ZS5 Lib AA069E1X Aven... 114 4e-026
gb|CN820119.1|CN820119 HRO4411_C12_F23ZS5 Lib AA069E1X Aven... 106 1e-023
>gb|CN820072.1|CN820072 HRO4410_G07_M14ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4410_G07_M14, mRNA
sequence
Length = 798
Score = 408 bits (885), Expect = e-114
Identities = 163/192 (84%), Positives = 183/192 (95%)
Frame = +3 / +2
Query: 396 KQTYYARFGTYLEHDIAHHTSGDHQKLLLAYMGIPRYEGPEVDPTIVTHDAKDLYKAGEK 575
+QTYY++FGTYLEHDI++ TSGDHQK+LLAY+GIPRYEGPEVDPTIVTHDAKDLYKAGEK
Sbjct: 11 RQTYYSKFGTYLEHDISYQTSGDHQKILLAYLGIPRYEGPEVDPTIVTHDAKDLYKAGEK 190
Query: 576 RLGTDEKIFIRVFTERSWAHLASVSSAYHHMYDRKLEKVIKSETSGNFEFALLTILRCAE 755
+ GTDEK FIR+FTERSWAH+A+V+SAYHHM+DR L KV+KSETSGNFE AL+TILRCAE
Sbjct: 191 KFGTDEKTFIRIFTERSWAHMAAVASAYHHMFDRSLVKVVKSETSGNFEVALVTILRCAE 370
Query: 756 NPAKYFAKLLRKAMKGLGTDDMTLIRVVVTRTEIDMQYIKAEYLKKYKKPLAEAINSETS 935
NPAKYFAK+LRK+MKGLGTDD TL+RVVVTRTEIDMQYIKAEY KKYKKPLA+AI+SETS
Sbjct: 371 NPAKYFAKVLRKSMKGLGTDDKTLVRVVVTRTEIDMQYIKAEYYKKYKKPLADAIHSETS 550
Query: 936 GNYRTFLLSLVG 971
G YRTFLLSLVG
Sbjct: 551 GGYRTFLLSLVG 586
Score = 314 bits (680), Expect = 2e-086
Identities = 146/193 (75%), Positives = 161/193 (83%)
Frame = -3 / -1
Query: 970 PTSERRNVR*FPDVSELIASANGFLYFFKYSALIYCMSISVLVTTTLISVMSSVPKPFMA 791
PTSE RNVR* PDVSE IASA+GFLYF YSA +YC+SISVLVTTTL SV+SSVP+PFM
Sbjct: 585 PTSESRNVR*PPDVSEWIASASGFLYFL*YSAFMYCISISVLVTTTLTSVLSSVPRPFMD 406
Query: 790 FRKSLAKYFAGFSAHLRIVKSANSKFPEVSLLITFSNFRSYI*W*AEETDAKCAQLRSVK 611
F +LAKYFAGFSAHLRIV SA SKFP+VSL TF+N RS + W*AE T A CAQLRSVK
Sbjct: 405 FLNTLAKYFAGFSAHLRIVTSATSKFPDVSLFTTFTNDRSNMWW*AEATAAMCAQLRSVK 226
Query: 610 TRIKIFSSVPSLFSPALYKSFASCVTIVGSTSGPS*RGIPIYASKSFW*SPDVW*AMSCS 431
R+K+FSSVP+ FSPALY+SFASCVTIVGSTSGPS*RG+P ASK F *SP V * MSCS
Sbjct: 225 IRMKVFSSVPNFFSPALYRSFASCVTIVGSTSGPS*RGMPR*ASKIF**SPLV**LMSCS 46
Query: 430 R*VPNLA**VCFI 392
R*VPNL * VC +
Sbjct: 45 R*VPNLE*YVCLV 7
Score = 217 bits (469), Expect(2) = 4e-058
Identities = 91/155 (58%), Positives = 110/155 (70%)
Frame = -2 / -3
Query: 863 HVNLGPRHHHPYKCHVISA*TFHGLS*ELSKILCWVFRASEDSQKCKFKVSRSFALDNLL 684
H+NLG +HHPYKC V+S TFHG * LSKIL W+ SEDS +C FK+SR F L NL
Sbjct: 478 HINLGSGYHHPYKCLVVSTKTFHGFP*HLSKILRWILSTSEDSYQCNFKISRCFTLHNLH 299
Query: 683 *FPVIHMMVSRRNRCQVCPTAFSENTDKNLLVRTQPFLTSFVQILCIMCHNCRINLGTFI 504
* P+ H++VSR N C VCP AFSE+TD+ L+ + LTSFVQ+LCIMCH INL FI
Sbjct: 298 *RPIKHVVVSRSNSCHVCPAAFSEDTDEGFLICAELLLTSFVQVLCIMCHYSGINLRPFI 119
Query: 503 AWNPHICK*KLLVITGCVVSNVMLKISTESSIISL 399
AWN I * LL+I C+++NVMLKIST+ II L
Sbjct: 118 AWNAEIG**DLLMIAACLITNVMLKISTKLGIICL 14
Score = 209 bits (451), Expect = 9e-055
Identities = 102/193 (52%), Positives = 133/193 (68%)
Frame = +2 / +1
Query: 392 NETDLLC*IRYLS*A*HCSPHIR*SPEAFTCIYGDSTL*RSRG*SYNCDT*CKGFVQSW* 571
+E D+L + YLS*A*H + R S E T + STL*R+ G*S+ DT*CKG VQSW*
Sbjct: 7 HEADILFQVWYLS*A*H*LSNKRRSSEDLTSLSRHSTL*RA*G*SHYSDT*CKGPVQSW* 186
Query: 572 EKAGYGREDFYPCFH*TQLGTLGICFFCLPSYV*PEIREGYQERNFWKL*ICTFDYPQMR 751
E+ + E+ +P H*TQLGT G C FCLP +V* + EG +E N WK * CT +YPQM
Sbjct: 187 EEVRHR*ENLHPYLH*TQLGTHGSCCFCLPPHV*SVVSEGCEE*NIWKF*SCTGNYPQMC 366
Query: 752 GKPSKVFC*ALTKGHERFRH**HDTYKGGGDEDRD*HAIYQGRVLEEV*EAIS*GYQLRD 931
+ S+VFC* + + HER +* DT KGGG+++RD*+AI++GRVL+E+ +A S* Y R+
Sbjct: 367 *ESSEVFC*GVKEIHERSWY*RQDTCKGGGNQNRD*YAIHKGRVLQEIQKAAS*CYPFRN 546
Query: 932 IRKLSDVPSFTCW 970
I LSDVP+ CW
Sbjct: 547 IGGLSDVPALACW 585
Score = 176 bits (379), Expect = 8e-045
Identities = 95/192 (49%), Positives = 115/192 (59%)
Frame = -1 / -2
Query: 969 QQVKEGTSDSFLMSRS**PQLMASYTSSSTLP*YIACQXXXXXXXXXXXSCHQCLNLSWP 790
QQ + GTSD+ M R+ * QL A S STLP IA Q SC Q +LSW
Sbjct: 584 QQARAGTSDNPPMFRNG*HQLAAFCISCSTLPLCIAYQSRFWLPPPLQVSCRQYQDLSWI 405
Query: 789 FVRA*QNTLLGFPRI*G*SKVQIQSFQKFRS**PSLISGHTYDGKQKKQMPSVPNCVQ*K 610
+ *QNT L I*G* VQ+Q+FQ F S PSL + T GKQK+Q+P VP+CVQ*+
Sbjct: 404 SLTP*QNTSLDSQHI*G*LPVQLQNFQMFHSSQPSLTTDQTCGGKQKQQLPCVPSCVQ*R 225
Query: 609 HG*KSSRPYPAFSHQLCTNPLHHVSQL*DQPRDLHSVESPYMQVKASGDHRMCGEQCHAQ 430
+G*+ S SHQLCT PLHHVS DQP+ LHSVE V++S D R+ CHAQ
Sbjct: 224 YG*RFSHLCRTSSHQLCTGPLHHVSL*WDQPQALHSVECRDRLVRSSDDRRLFDN*CHAQ 45
Query: 429 DKYRI*HNKSVS 394
DKY+ +N S S
Sbjct: 44 DKYQTWNNMSAS 9
Score = 171 bits (367), Expect(2) = 6e-044
Identities = 90/156 (57%), Positives = 99/156 (63%)
Frame = +1 / +3
Query: 400 RLIMLDSVLILSMTLLTTHPVITRSFYLHIWGFHAMKVPRLILQL*HMMQRICTKLVRKG 579
R I+ VLILSMTL+ I R Y I FHAMK RLI *HMMQR CTKLVR+
Sbjct: 15 RHIIPSLVLILSMTLVIKQAAIIRRSY*PISAFHAMKGLRLIPL**HMMQRTCTKLVRRS 194
Query: 580 WVRTRRFLSVFSLNAVGHTWHLFLLLTIICMTGN*RRLSRAKLLETLNLHF*LSSDARKT 759
+ R+ SV SLNA GHTW L LLLT C+ G * RL R K LE L LH+*LSSD +
Sbjct: 195 SAQMRKPSSVSSLNAAGHTWQLLLLLTTTCLIGR**RL*RVKHLEILKLHW*LSSDVLRI 374
Query: 760 QQSILLSSYERP*KV*ALMT*HL*GWW*RGPRLTCN 867
Q+SILL P*KV L T HL*GWW* PRL CN
Sbjct: 375 QRSILLRC*GNP*KVLVLTTRHL*GWW*PEPRLICN 482
Score = 23.5 bits (45), Expect(2) = 4e-058
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = -2 / -3
Query: 971 TNK*KKERPIVS*CLG 924
TNK ++ERPI C G
Sbjct: 586 TNKREQERPITPRCFG 539
Score = 22.6 bits (43), Expect(2) = 6e-044
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1 / +3
Query: 925 PRHQETIGRSFFHLLV 972
P+H+ IGRS LLV
Sbjct: 540 PKHRGVIGRSCSRLLV 587
>gb|CN818277.1|CN818277 HRO4470_C12_F24ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4470_C12_F24, mRNA sequence
Length = 620
Score = 295 bits (639), Expect(2) = 8e-084
Identities = 121/142 (85%), Positives = 134/142 (94%)
Frame = +3 / +1
Query: 546 AKDLYKAGEKRLGTDEKIFIRVFTERSWAHLASVSSAYHHMYDRKLEKVIKSETSGNFEF 725
AKDLYKAGEK+ GTDEK FIR+FTERSWAH+A+V+SAYHHM+DR L KV+KSETSGNFE
Sbjct: 55 AKDLYKAGEKKFGTDEKTFIRIFTERSWAHMAAVASAYHHMFDRSLVKVVKSETSGNFEV 234
Query: 726 ALLTILRCAENPAKYFAKLLRKAMKGLGTDDMTLIRVVVTRTEIDMQYIKAEYLKKYKKP 905
AL+TILRCAENPAKYFAK+LRK+MKGLGTDD TL+RVVVTRTEIDMQYIKAEY KKYKKP
Sbjct: 235 ALVTILRCAENPAKYFAKVLRKSMKGLGTDDKTLVRVVVTRTEIDMQYIKAEYYKKYKKP 414
Query: 906 LAEAINSETSGNYRTFLLSLVG 971
LA+AI+SETSG YRTFLLSLVG
Sbjct: 415 LADAIHSETSGGYRTFLLSLVG 480
Score = 240 bits (519), Expect(2) = 1e-065
Identities = 107/142 (75%), Positives = 120/142 (84%)
Frame = -3 / -1
Query: 970 PTSERRNVR*FPDVSELIASANGFLYFFKYSALIYCMSISVLVTTTLISVMSSVPKPFMA 791
PTSE RNVR* PDVSE IASA+GFLYF YSA +YC+SISVLVTTTL SV+SSVP+PFM
Sbjct: 479 PTSESRNVR*PPDVSEWIASASGFLYFL*YSAFMYCISISVLVTTTLTSVLSSVPRPFMD 300
Query: 790 FRKSLAKYFAGFSAHLRIVKSANSKFPEVSLLITFSNFRSYI*W*AEETDAKCAQLRSVK 611
F +LAKYFAGFSAHLRIV SA SKFP+VSL TF+N RS + W*AE T A CAQLRSVK
Sbjct: 299 FLNTLAKYFAGFSAHLRIVTSATSKFPDVSLFTTFTNDRSNMWW*AEATAAMCAQLRSVK 120
Query: 610 TRIKIFSSVPSLFSPALYKSFA 545
R+K+FSSVP+ FSPALY+SFA
Sbjct: 119 IRMKVFSSVPNFFSPALYRSFA 54
Score = 173 bits (373), Expect = 5e-044
Identities = 77/142 (54%), Positives = 101/142 (71%)
Frame = +2 / +3
Query: 545 CKGFVQSW*EKAGYGREDFYPCFH*TQLGTLGICFFCLPSYV*PEIREGYQERNFWKL*I 724
CKG VQSW*E+ + E+ +P H*TQLGT G C FCLP +V* + EG +E N WK *
Sbjct: 54 CKGPVQSW*EEVRHR*ENLHPYLH*TQLGTHGSCCFCLPPHV*SVVSEGCEE*NIWKF*S 233
Query: 725 CTFDYPQMRGKPSKVFC*ALTKGHERFRH**HDTYKGGGDEDRD*HAIYQGRVLEEV*EA 904
CT +YPQM + S+VFC* + + HER +* DT KGGG+++RD*+AI++GRVL+E+ +A
Sbjct: 234 CTGNYPQMC*ESSEVFC*GVKEIHERSWY*RQDTCKGGGNQNRD*YAIHKGRVLQEIQKA 413
Query: 905 IS*GYQLRDIRKLSDVPSFTCW 970
S* Y R+I LSDVP+ CW
Sbjct: 414 AS*CYPFRNIGGLSDVPALACW 479
Score = 158 bits (340), Expect(2) = 2e-040
Identities = 63/107 (58%), Positives = 77/107 (71%)
Frame = -2 / -3
Query: 863 HVNLGPRHHHPYKCHVISA*TFHGLS*ELSKILCWVFRASEDSQKCKFKVSRSFALDNLL 684
H+NLG +HHPYKC V+S TFHG * LSKIL W+ SEDS +C FK+SR F L NL
Sbjct: 372 HINLGSGYHHPYKCLVVSTKTFHGFP*HLSKILRWILSTSEDSYQCNFKISRCFTLHNLH 193
Query: 683 *FPVIHMMVSRRNRCQVCPTAFSENTDKNLLVRTQPFLTSFVQILCI 543
* P+ H++VSR N C VCP AFSE+TD+ L+ + LTSFVQ+LCI
Sbjct: 192 *RPIKHVVVSRSNSCHVCPAAFSEDTDEGFLICAELLLTSFVQVLCI 52
Score = 133 bits (286), Expect(2) = 7e-033
Identities = 65/108 (60%), Positives = 72/108 (66%)
Frame = +1 / +2
Query: 544 MQRICTKLVRKGWVRTRRFLSVFSLNAVGHTWHLFLLLTIICMTGN*RRLSRAKLLETLN 723
MQR CTKLVR+ + R+ SV SLNA GHTW L LLLT C+ G * RL R K LE L
Sbjct: 53 MQRTCTKLVRRSSAQMRKPSSVSSLNAAGHTWQLLLLLTTTCLIGR**RL*RVKHLEILK 232
Query: 724 LHF*LSSDARKTQQSILLSSYERP*KV*ALMT*HL*GWW*RGPRLTCN 867
LH+*LSSD + Q+SILL P*KV L T HL*GWW* PRL CN
Sbjct: 233 LHW*LSSDVLRIQRSILLRC*GNP*KVLVLTTRHL*GWW*PEPRLICN 376
Score = 126 bits (270), Expect = 8e-030
Identities = 70/142 (49%), Positives = 84/142 (59%)
Frame = -1 / -2
Query: 969 QQVKEGTSDSFLMSRS**PQLMASYTSSSTLP*YIACQXXXXXXXXXXXSCHQCLNLSWP 790
QQ + GTSD+ M R+ * QL A S STLP IA Q SC Q +LSW
Sbjct: 478 QQARAGTSDNPPMFRNG*HQLAAFCISCSTLPLCIAYQSRFWLPPPLQVSCRQYQDLSWI 299
Query: 789 FVRA*QNTLLGFPRI*G*SKVQIQSFQKFRS**PSLISGHTYDGKQKKQMPSVPNCVQ*K 610
+ *QNT L I*G* VQ+Q+FQ F S PSL + T GKQK+Q+P VP+CVQ*+
Sbjct: 298 SLTP*QNTSLDSQHI*G*LPVQLQNFQMFHSSQPSLTTDQTCGGKQKQQLPCVPSCVQ*R 119
Query: 609 HG*KSSRPYPAFSHQLCTNPLH 544
+G*+ S SHQLCT PLH
Sbjct: 118 YG*RFSHLCRTSSHQLCTGPLH 53
Score = 31.3 bits (62), Expect(2) = 8e-084
Identities = 12/16 (75%), Positives = 13/16 (81%)
Frame = +3 / +3
Query: 504 YEGPEVDPTIVTHDAK 551
YEG +VDPTIVTH K
Sbjct: 12 YEGLQVDPTIVTHVCK 59
Score = 25.8 bits (50), Expect(2) = 1e-065
Identities = 11/14 (78%), Positives = 12/14 (85%)
Frame = -3 / -2
Query: 544 SCVTIVGSTSGPS* 503
+CVTIVGST PS*
Sbjct: 52 TCVTIVGST*SPS* 11
Score = 23.5 bits (45), Expect(2) = 2e-040
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = -2 / -3
Query: 971 TNK*KKERPIVS*CLG 924
TNK ++ERPI C G
Sbjct: 480 TNKREQERPITPRCFG 433
Score = 22.6 bits (43), Expect(2) = 7e-033
Identities = 9/16 (56%), Positives = 11/16 (68%)
Frame = +1 / +2
Query: 925 PRHQETIGRSFFHLLV 972
P+H+ IGRS LLV
Sbjct: 434 PKHRGVIGRSCSRLLV 481
>gb|CN816332.1|CN816332 HRO4519_E08_J15ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4519_E08_J15, mRNA sequence
Length = 546
Score = 150 bits (323), Expect = 4e-037
Identities = 64/71 (90%), Positives = 68/71 (95%)
Frame = +3 / +3
Query: 759 PAKYFAKLLRKAMKGLGTDDMTLIRVVVTRTEIDMQYIKAEYLKKYKKPLAEAINSETSG 938
PAKYFAK+LRK+MKGLGTDD TLIRVVVTRTEIDMQYIKAEY KKYKKPLA+AI+SETSG
Sbjct: 12 PAKYFAKVLRKSMKGLGTDDKTLIRVVVTRTEIDMQYIKAEYYKKYKKPLADAIHSETSG 191
Query: 939 NYRTFLLSLVG 971
YRTFLLSLVG
Sbjct: 192 GYRTFLLSLVG 224
Score = 124 bits (266), Expect = 3e-029
Identities = 55/71 (77%), Positives = 61/71 (85%)
Frame = -3 / -3
Query: 970 PTSERRNVR*FPDVSELIASANGFLYFFKYSALIYCMSISVLVTTTLISVMSSVPKPFMA 791
PTSE RNVR* PDVSE IASA+GFLYF YSA +YC+SISVLVTTTLI V+SSVP+PFM
Sbjct: 223 PTSESRNVR*PPDVSEWIASASGFLYFL*YSAFMYCISISVLVTTTLIRVLSSVPRPFMD 44
Query: 790 FRKSLAKYFAG 758
F +LAKYFAG
Sbjct: 43 FLNTLAKYFAG 11
>gb|CN820263.1|CN820263 HRO4417_D05_G09ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4417_D05_G09, mRNA
sequence
Length = 592
Score = 114 bits (243), Expect = 4e-026
Identities = 49/155 (31%), Positives = 87/155 (56%)
Frame = +3 / +1
Query: 528 TIVTHDAKDLYKAGEKRLGTDEKIFIRVFTERSWAHLASVSSAYHHMYDRKLEKVIKSET 707
T V H L + ++ IR+ T RS A L + ++Y+ + + K +K +
Sbjct: 34 TSVAHSEAKLLHGKIQEKAYGDEEIIRILTTRSKAQLLATFNSYNDQFSHPITKDLKEDP 213
Query: 708 SGNFEFALLTILRCAENPAKYFAKLLRKAMKGLGTDDMTLIRVVVTRTEIDMQYIKAEYL 887
F L I+RC P +YF K++R A+ G+GTD+ +L R++ TR E+D++ IK YL
Sbjct: 214 KDEFLATLRAIIRCFACPDRYFEKIIRLALGGVGTDENSLTRIITTRAEVDLKLIKEAYL 393
Query: 888 KKYKKPLAEAINSETSGNYRTFLLSLVGHGH*AMF 992
K+ PL +A++ +T+ +Y +L+L+G +* ++
Sbjct: 394 KRNSVPLEKAVSKDTTRDYEDMMLALLGAEY*GVW 498
>gb|CN820119.1|CN820119 HRO4411_C12_F23ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4411_C12_F23, mRNA
sequence
Length = 480
Score = 106 bits (226), Expect = 1e-023
Identities = 45/50 (90%), Positives = 48/50 (96%)
Frame = +3 / +1
Query: 822 TLIRVVVTRTEIDMQYIKAEYLKKYKKPLAEAINSETSGNYRTFLLSLVG 971
TL+RVVVTRTEIDMQYIKAEY KKYKKPLA+AI+SETSG YRTFLLSLVG
Sbjct: 13 TLVRVVVTRTEIDMQYIKAEYYKKYKKPLADAIHSETSGGYRTFLLSLVG 162
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 18,336,603
Number of Sequences: 8143
Number of extensions: 287482
Number of successful extensions: 1497
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 534468140
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)