TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3696537.2.1
(592 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816396.1|CN816396 HRO4520_C05_F10ZS5 Lib AA071E1X Aven... 116 4e-038
gb|CN816242.1|CN816242 HRO4518_E06_I12ZS5 Lib AA071E1X Aven... 116 4e-038
gb|CN814888.1|CN814888 HRO4503_E12_J23ZS5 Lib AA071E1X Aven... 91 5e-034
>gb|CN816396.1|CN816396 HRO4520_C05_F10ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_C05_F10, mRNA sequence
Length = 851
Score = 116 bits (247), Expect(2) = 4e-038
Identities = 38/69 (55%), Positives = 52/69 (75%)
Frame = -2 / +2
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQK 412
MHH+CP+C EYLFDS+ +SVL CGHTIH CL EM H++ CP+C +S C+MS W++
Sbjct: 485 MHHDCPICFEYLFDSIDGVSVLRCGHTIHENCLKEMEKHRKLICPLCSKSVCDMSRAWER 664
Query: 411 LDQQVAASP 385
LD+++A P
Sbjct: 665 LDREIARLP 691
Score = 56.5 bits (117), Expect(2) = 4e-038
Identities = 20/42 (47%), Positives = 29/42 (69%)
Frame = -2 / +2
Query: 384 MPAIYQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQ 259
+P Y +MV ILCN+C + V+F+++A KC C SYNTR+
Sbjct: 686 LPDSYDGRMVRILCNECEAIAEVRFNLIALKCQSCQSYNTRR 811
>gb|CN816242.1|CN816242 HRO4518_E06_I12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4518_E06_I12, mRNA sequence
Length = 723
Score = 116 bits (247), Expect(2) = 4e-038
Identities = 38/69 (55%), Positives = 52/69 (75%)
Frame = -2 / +3
Query: 591 MHHNCPVCMEYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQK 412
MHH+CP+C EYLFDS+ +SVL CGHTIH CL EM H++ CP+C +S C+MS W++
Sbjct: 42 MHHDCPICFEYLFDSIDGVSVLRCGHTIHENCLKEMEKHRKLICPLCSKSVCDMSRAWER 221
Query: 411 LDQQVAASP 385
LD+++A P
Sbjct: 222 LDREIARLP 248
Score = 56.5 bits (117), Expect(2) = 4e-038
Identities = 20/42 (47%), Positives = 29/42 (69%)
Frame = -2 / +3
Query: 384 MPAIYQKKMVWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQ 259
+P Y +MV ILCN+C + V+F+++A KC C SYNTR+
Sbjct: 243 LPDSYDGRMVRILCNECEAIAEVRFNLIALKCQSCQSYNTRR 368
>gb|CN814888.1|CN814888 HRO4503_E12_J23ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_E12_J23, mRNA sequence
Length = 751
Score = 91.3 bits (193), Expect(2) = 5e-034
Identities = 32/69 (46%), Positives = 49/69 (71%)
Frame = -2 / +1
Query: 564 EYLFDSMKAISVLHCGHTIHLECLYEMRAHQQFSCPVCLRSACNMSDIWQKLDQQVAASP 385
E LFDS+K VL CGHT+H++C EM H ++SCP+C ++A +M+ W LDQ++ A+
Sbjct: 7 EDLFDSLKGTRVLTCGHTMHMDCFSEMVEHNKYSCPICSKTALDMTHHWGMLDQEIEATI 186
Query: 384 MPAIYQKKM 358
MP +Y+ K+
Sbjct: 187 MPPVYRYKV 213
Score = 67.5 bits (141), Expect(2) = 5e-034
Identities = 22/34 (64%), Positives = 25/34 (73%)
Frame = -2 / +1
Query: 357 VWILCNDCGVTSNVQFHILAHKCPGCSSYNTRQT 256
VW+LCNDC S V FH++ HKC CSSYNTR T
Sbjct: 211 VWVLCNDCNKVSEVDFHVIGHKCSHCSSYNTRST 312
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,108,664
Number of Sequences: 8143
Number of extensions: 152948
Number of successful extensions: 2531
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 188724074
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)