TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3570920.2.1
(786 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|AF155932.1| Avena sativa 1,3-beta glucanase (Oglc13) mRN... 58 6e-037
gb|AY277385.1| Avena sativa 1,3-beta glucanase (Oglc13) gen... 55 2e-036
emb|Z15131.1|ASBGLUCAN A.sativa mRNA for beta glucanase 64 3e-027
gb|CN821029.1|CN821029 HRO4422_G03_M06ZS5 Lib AA069E1X Aven... 54 3e-021
>gb|AF155932.1| Avena sativa 1,3-beta glucanase (Oglc13) mRNA, partial cds
Length = 1045
Score = 57.6 bits (120), Expect(6) = 6e-037
Identities = 21/46 (45%), Positives = 32/46 (69%)
Frame = +1 / +1
Query: 352 VIDPNTGLVYTNMFDAQVDSIFFALMALNFKTLKIMITESGWPNKG 489
V D +GL YTN+FDA VD++ AL ++K++++ESGWP +G
Sbjct: 574 VRDSKSGLTYTNLFDAMVDAVHAALEKAGAASVKVVVSESGWPKEG 711
Score = 49.0 bits (101), Expect(6) = 6e-037
Identities = 18/45 (40%), Positives = 30/45 (66%)
Frame = +1 / +1
Query: 571 GTPAKPGEEIDVYIFSLFNENRKPGIESERNWGLFFPDKSSIYSL 705
G K ++ Y+F++F+EN+K G +E+++GLF PDKS Y +
Sbjct: 775 GGTPKKRGALETYVFAMFDENQKTGAGTEKHFGLFNPDKSPAYPI 909
Score = 43.9 bits (90), Expect(6) = 6e-037
Identities = 13/42 (30%), Positives = 27/42 (64%)
Frame = +1 / +1
Query: 214 FLKPMLQFLVENQAPFMVDLYPYYAYQNSRSNVSLNYALFSP 339
++ + + L AP + ++YPY++Y+++ ++ LNYA F P
Sbjct: 439 YMTDVARLLASTGAPLLANIYPYFSYRDNPRDIQLNYATFRP 564
Score = 30.7 bits (61), Expect(6) = 6e-037
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +1 / +1
Query: 502 TGATPDNAQTYNTNLIRHV 558
TGA+ DNA+ YN LI HV
Sbjct: 715 TGASVDNARAYNQGLIDHV 771
Score = 30.3 bits (60), Expect(6) = 6e-037
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +1 / +1
Query: 127 ITISSTHSLGILSRSFPPSAGAFNSSY 207
I +S+ ++ SFPPSAG F SY
Sbjct: 361 IKVSTAIRFDAVANSFPPSAGVFAQSY 441
Score = 27.1 bits (53), Expect(6) = 6e-037
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = +1 / +1
Query: 67 VVPAMRNVHTALKKAGL 117
+VPA+RN++ AL AGL
Sbjct: 304 IVPAIRNLNAALSAAGL 354
>gb|AY277385.1| Avena sativa 1,3-beta glucanase (Oglc13) gene, complete cds
Length = 3281
Score = 55.3 bits (115), Expect(6) = 2e-036
Identities = 21/46 (45%), Positives = 30/46 (65%)
Frame = +1 / +2
Query: 352 VIDPNTGLVYTNMFDAQVDSIFFALMALNFKTLKIMITESGWPNKG 489
V D +GL YTN+FDA VD++ AL +K++++ESGWP G
Sbjct: 2819 VRDSKSGLTYTNLFDAMVDAVHAALEKAGAPGVKVVVSESGWPKTG 2956
Score = 49.0 bits (101), Expect(6) = 2e-036
Identities = 18/45 (40%), Positives = 30/45 (66%)
Frame = +1 / +2
Query: 571 GTPAKPGEEIDVYIFSLFNENRKPGIESERNWGLFFPDKSSIYSL 705
G K ++ Y+F++F+EN+K G +E+++GLF PDKS Y +
Sbjct: 3020 GGTPKKRGALETYVFAMFDENQKTGAGTEKHFGLFNPDKSPAYPI 3154
Score = 43.9 bits (90), Expect(6) = 2e-036
Identities = 13/42 (30%), Positives = 27/42 (64%)
Frame = +1 / +2
Query: 214 FLKPMLQFLVENQAPFMVDLYPYYAYQNSRSNVSLNYALFSP 339
++ + + L AP + ++YPY++Y+++ ++ LNYA F P
Sbjct: 2684 YMTDVARLLASTGAPLLANIYPYFSYRDNPRDIQLNYATFRP 2809
Score = 31.2 bits (62), Expect(6) = 2e-036
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +1 / +2
Query: 502 TGATPDNAQTYNTNLIRHV 558
TGA+ DNA+ YN LI HV
Sbjct: 2960 TGASTDNARAYNQGLIDHV 3016
Score = 29.8 bits (59), Expect(6) = 2e-036
Identities = 12/27 (44%), Positives = 16/27 (59%)
Frame = +1 / +2
Query: 127 ITISSTHSLGILSRSFPPSAGAFNSSY 207
I +S+ ++ SFPPSAG F SY
Sbjct: 2606 IKVSTAIRFDAVASSFPPSAGVFAQSY 2686
Score = 27.1 bits (53), Expect(6) = 2e-036
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = +1 / +2
Query: 67 VVPAMRNVHTALKKAGL 117
+VPA+RN++ AL AGL
Sbjct: 2549 IVPAIRNLNAALSAAGL 2599
>emb|Z15131.1|ASBGLUCAN A.sativa mRNA for beta glucanase
Length = 1448
Score = 64.0 bits (134), Expect(5) = 3e-027
Identities = 28/78 (35%), Positives = 45/78 (57%)
Frame = +1 / +1
Query: 127 ITISSTHSLGILSRSFPPSAGAFNSSYAYFLKPMLQFLVENQAPFMVDLYPYYAYQNSRS 306
I ++++ S IL PPSAG+F F+ P++QFL +P M ++YPY A+ + S
Sbjct: 472 IKVTTSVSQAILGVYSPPSAGSFTGEADAFMGPVVQFLARTGSPLMANIYPYLAWAYNPS 651
Query: 307 NVSLNYALFSPESQDVID 360
+ ++YALF+ V D
Sbjct: 652 AMDMSYALFTASGTVVQD 705
Score = 41.7 bits (85), Expect(5) = 3e-027
Identities = 16/44 (36%), Positives = 26/44 (59%)
Frame = +1 / +1
Query: 379 YTNMFDAQVDSIFFALMALNFKTLKIMITESGWPNKGAVKETGA 510
Y N+FD VD+ + A+ +K++++ESGWP+ G T A
Sbjct: 718 YQNLFDTTVDAFYTAMGKHGGAGVKLVVSESGWPSAGGEAATPA 849
Score = 30.3 bits (60), Expect(5) = 3e-027
Identities = 8/19 (42%), Positives = 14/19 (73%)
Frame = +1 / +1
Query: 655 ERNWGLFFPDKSSIYSLDW 711
E+NWGLF+P+ +Y + +
Sbjct: 973 EQNWGLFYPNMQHVYPISF 1029
Score = 27.5 bits (54), Expect(5) = 3e-027
Identities = 8/14 (57%), Positives = 13/14 (92%)
Frame = +1 / +1
Query: 598 IDVYIFSLFNENRK 639
I+ Y+F++FNEN+K
Sbjct: 919 IETYVFAMFNENQK 960
Score = 24.8 bits (48), Expect(5) = 3e-027
Identities = 10/17 (58%), Positives = 13/17 (76%)
Frame = +1 / +1
Query: 67 VVPAMRNVHTALKKAGL 117
++PAM+NV AL AGL
Sbjct: 415 LLPAMQNVQGALASAGL 465
>gb|CN821029.1|CN821029 HRO4422_G03_M06ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4422_G03_M06, mRNA
sequence
Length = 508
Score = 53.5 bits (111), Expect(3) = 3e-021
Identities = 20/36 (55%), Positives = 29/36 (80%)
Frame = +1 / +3
Query: 598 IDVYIFSLFNENRKPGIESERNWGLFFPDKSSIYSL 705
I+ YIF++FNEN+K G E+E+++GLF DKS YS+
Sbjct: 240 IETYIFAMFNENKKTGDETEKHFGLFNTDKSPAYSI 347
Score = 47.6 bits (98), Expect(3) = 3e-021
Identities = 18/44 (40%), Positives = 28/44 (63%)
Frame = +1 / +3
Query: 379 YTNMFDAQVDSIFFALMALNFKTLKIMITESGWPNKGAVKETGA 510
Y N+FDA VD+ + AL + +KI+++ESGWP+ G + A
Sbjct: 39 YQNLFDALVDTFYSALGSAGAGNVKIVVSESGWPSAGGTAASAA 170
Score = 32.6 bits (65), Expect(3) = 3e-021
Identities = 13/19 (68%), Positives = 14/19 (73%)
Frame = +1 / +3
Query: 502 TGATPDNAQTYNTNLIRHV 558
T A+ NAQTYN NLI HV
Sbjct: 153 TAASAANAQTYNQNLINHV 209
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.316 0.132 0.390
Matrix: BLOSUM62
Number of Hits to DB: 9,324,085
Number of Sequences: 8143
Number of extensions: 142712
Number of successful extensions: 1531
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 262395315
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.6 bits)