TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3438088.2.1
(615 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821143.1|CN821143 HRO4428_A02_B04ZS5 Lib AA069E1X Aven... 171 1e-044
gb|CN821108.1|CN821108 HRO4427_F02_L03ZS5 Lib AA069E1X Aven... 171 2e-044
>gb|CN821143.1|CN821143 HRO4428_A02_B04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4428_A02_B04, mRNA
sequence
Length = 636
Score = 171 bits (367), Expect(2) = 1e-044
Identities = 62/77 (80%), Positives = 69/77 (89%)
Frame = -3 / +2
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVG+GK+YP+ VHHRGASIP NGVHYGCKGG+KWR++KK NPN + GAMVAGP
Sbjct: 53 NPQKMSYVVGYGKKYPKRVHHRGASIPHNGVHYGCKGGFKWRESKKANPNILVGAMVAGP 232
Query: 433 DRHDGFKDARRNYNYTE 383
DRHDGFKD R NYNYTE
Sbjct: 233 DRHDGFKDIRTNYNYTE 283
Score = 116 bits (247), Expect = 5e-027
Identities = 57/90 (63%), Positives = 63/90 (70%)
Frame = +3 / -1
Query: 345 NAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP**T 524
+AAT+PA A V SV*L*F+R SL PS SGPAT+AP + GF FL SLH PPL P* T
Sbjct: 321 SAATKPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRIFGFAFLDSLHLKPPLHP*WT 142
Query: 525 PFLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
P GI+AP *T G FP PTT*LIF GF
Sbjct: 141 PL*GIDAPL**TRFGYFFP*PTT*LIFCGF 52
Score = 23.5 bits (45), Expect(2) = 1e-044
Identities = 9/10 (90%), Positives = 10/10 (100%)
Frame = -3 / +2
Query: 313 VDKNTMFSAV 284
VDKNT+FSAV
Sbjct: 362 VDKNTIFSAV 391
>gb|CN821108.1|CN821108 HRO4427_F02_L03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_F02_L03, mRNA
sequence
Length = 714
Score = 171 bits (367), Expect(2) = 2e-044
Identities = 62/77 (80%), Positives = 69/77 (89%)
Frame = -3 / +2
Query: 613 NPLKMSYVVGFGKRYPRHVHHRGASIPKNGVHYGCKGGWKWRDAKKPNPNTIAGAMVAGP 434
NP KMSYVVG+GK+YP+ VHHRGASIP NGVHYGCKGG+KWR++KK NPN + GAMVAGP
Sbjct: 296 NPQKMSYVVGYGKKYPKRVHHRGASIPHNGVHYGCKGGFKWRESKKANPNILVGAMVAGP 475
Query: 433 DRHDGFKDARRNYNYTE 383
DRHDGFKD R NYNYTE
Sbjct: 476 DRHDGFKDIRTNYNYTE 526
Score = 117 bits (251), Expect = 1e-027
Identities = 58/90 (64%), Positives = 64/90 (71%)
Frame = +3 / -1
Query: 345 NAATRPALPASVASV*L*FLRASLNPSWRSGPATMAPAMVLGFGFLASLHFHPPLQP**T 524
+AAT+PA A V SV*L*F+R SL PS SGPAT+AP + GF FL SLH PPL P* T
Sbjct: 564 SAATKPAFAARVGSV*L*FVRISLKPSCLSGPATIAPTRIFGFAFLDSLHLKPPLHP*WT 385
Query: 525 PFLGIEAPRW*TCLG*RFPNPTT*LIFKGF 614
P GI+AP *T LG FP PTT*LIF GF
Sbjct: 384 PL*GIDAPL**TRLGYFFP*PTT*LIFCGF 295
Score = 23.1 bits (44), Expect(2) = 2e-044
Identities = 8/10 (80%), Positives = 10/10 (100%)
Frame = -3 / +2
Query: 313 VDKNTMFSAV 284
+DKNT+FSAV
Sbjct: 605 IDKNTIFSAV 634
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,839,541
Number of Sequences: 8143
Number of extensions: 124195
Number of successful extensions: 1825
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 198527922
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)