TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3345642.2.1
(600 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Aven... 255 5e-069
gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Aven... 220 2e-058
gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Aven... 133 2e-032
gb|CN814825.1|CN814825 HRO4502_H06_O12ZS5 Lib AA071E1X Aven... 90 2e-025
gb|CN815983.1|CN815983 HRO4515_F02_L03ZS5 Lib AA071E1X Aven... 90 5e-025
>gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4423_D02_H03, mRNA
sequence
Length = 733
Score = 255 bits (551), Expect = 5e-069
Identities = 93/156 (59%), Positives = 115/156 (73%)
Frame = +1 / +1
Query: 4 RGNVIGAVETCLESFHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSL 183
+G V GAVETC ESFHR+ KYLNFS+P I+ DP+ CGWA+GMNIFDL W+ + T +
Sbjct: 91 KGKVNGAVETCGESFHRFDKYLNFSNPHIARNFDPNACGWAYGMNIFDLKQWKNKDITGI 270
Query: 184 YHYWQEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVH 363
YH WQ N D +LW+ GTLP GL+TFY L PLD+ WHVLGLGY+ ID I++AAV H
Sbjct: 271 YHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPSIDRSEIDNAAVAH 450
Query: 364 YNGNMKPWLKLAIRRYKYIWERYVNISHPYVRECML 471
YNGNMKPWL+LA+ +Y+ W RY+ HPY+R C L
Sbjct: 451 YNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNL 558
Score = 95.0 bits (201), Expect = 1e-020
Identities = 47/151 (31%), Positives = 89/151 (58%)
Frame = -1 / -2
Query: 459 SNIRV*DIHIPFPYILVTANSQLQPGFHVSIIVHNSSTLNQTIIYVYIIPETQDMPSAV* 280
++IR+ ++IP P + + QLQP H+ I++ NSS + +Y +++ ++DMP +
Sbjct: 546 ADIRMIILYIPCPVWPILGHCQLQPWLHIPIVMSNSSIVYLRAVYAWVVS*SKDMPRLIK 367
Query: 279 GLHQAIKCQKACRKSSCSPEQ*VRILLLPIMIQ*CGVCLPPSYQVKNVHSKSPSTSMWIN 100
G+ Q ++ +A K P+ V I +LP+++ + + P +K+VH SP+TS+ I
Sbjct: 366 GMCQLVERHEARWKCPELPKNPVFIHVLPLVVDPGNIFVLPLL*IKDVHPISPTTSIRIK 187
Query: 99 LGADSRMRKI*ILVIPMKRL*TSFHCSNDIA 7
+ ++ R+ KI ILV ++ L T HC+ D++
Sbjct: 186 VPSNMRI*KIKILVKTVETLSTGLHCTIDLS 94
Score = 80.8 bits (170), Expect(2) = 2e-028
Identities = 38/69 (55%), Positives = 42/69 (60%)
Frame = -2 / -3
Query: 401 IASFSQGFMFPL*CTTAALSIRRSSMSTSYPRPKTCHLRSRGSIRP*NVKRPAGRVPVLQ 222
IAS + GF+FPL* TAALSI SM YP P+TCH SRG + *NV RP G VP Q
Sbjct: 488 IASSNHGFIFPL**ATAALSISERSMLGLYPNPRTCHDLSRGCVSL*NVMRPGGSVPSFQ 309
Query: 221 SNRSEFCSC 195
S F C
Sbjct: 308 RTLSSFMFC 282
Score = 59.3 bits (123), Expect(2) = 2e-028
Identities = 29/53 (54%), Positives = 31/53 (58%)
Frame = -2 / -3
Query: 164 FLQAIKSKMFIPKAHPQVCGSILELIVGCEKFRYL*YR*NDSKQVSTAPMTLP 6
F +SKMFIP A PQ GS I G EK RYL R S QVSTAP+T P
Sbjct: 251 FFHCFRSKMFIP*AQPQALGSKFRAI*GFEKLRYLSKRWKLSPQVSTAPLTFP 93
>gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_A06_B12, mRNA sequence
Length = 570
Score = 220 bits (475), Expect = 2e-058
Identities = 77/142 (54%), Positives = 103/142 (72%)
Frame = +1 / +1
Query: 46 FHRYHKYLNFSHPTISSKIDPHTCGWAFGMNIFDLIAWRKANATSLYHYWQEQNSDLLLW 225
F R+ KYLNFS+P I+ D + CGWA+GMN+FDL W+K + T +YH WQ N + LLW
Sbjct: 22 FPRFDKYLNFSNPNIAQNFDANACGWAYGMNMFDLEEWKKKDITGIYHRWQTLNENRLLW 201
Query: 226 RTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGNMKPWLKLAIR 405
+ GTLP GL+TFY L PLD+ WHVLGLGY+ ++ I+SAAV+HYNGNMKPWL++A+
Sbjct: 202 KLGTLPPGLMTFYKLTHPLDKSWHVLGLGYNPTVEHSEIDSAAVIHYNGNMKPWLEIAMT 381
Query: 406 RYKYIWERYVNISHPYVRECML 471
+Y+ W +Y+N H YV C +
Sbjct: 382 KYRPYWTKYINYEHSYVHGCKI 447
>gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_D01_H01, mRNA sequence
Length = 653
Score = 133 bits (286), Expect = 2e-032
Identities = 46/90 (51%), Positives = 66/90 (73%)
Frame = +1 / +1
Query: 196 QEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGN 375
Q QN + LLW+ GTLP GL+TFY +PLD+ WHVLGLGY+ I I +AAVVH+NGN
Sbjct: 331 QTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGN 510
Query: 376 MKPWLKLAIRRYKYIWERYVNISHPYVREC 465
MKPWL + + +++++W +YV+ ++R+C
Sbjct: 511 MKPWLDIGMNQFRHLWTKYVDYGDSFIRQC 600
>gb|CN814825.1|CN814825 HRO4502_H06_O12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_H06_O12, mRNA sequence
Length = 837
Score = 90.4 bits (191), Expect(2) = 2e-025
Identities = 38/90 (42%), Positives = 51/90 (56%)
Frame = +1 / +1
Query: 196 QEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGN 375
Q + D + R LL+F L+ PLD R + GLGYD ID + S+A +HYNGN
Sbjct: 268 QVKIKDEVALREAAFSISLLSFQHLIYPLDERLTLSGLGYDYGIDPEVARSSASLHYNGN 447
Query: 376 MKPWLKLAIRRYKYIWERYVNISHPYVREC 465
MKPWL+L I YK W R++ ++ EC
Sbjct: 448 MKPWLELGILEYKKYWRRFLTREDRFMDEC 537
Score = 39.6 bits (80), Expect(2) = 2e-025
Identities = 13/31 (41%), Positives = 18/31 (58%)
Frame = +1 / +1
Query: 94 SKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
+K DP +C W G+N+ +L WRK T Y
Sbjct: 160 AKYDPKSCAWMSGVNVINLDKWRKHKVTENY 252
>gb|CN815983.1|CN815983 HRO4515_F02_L03ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_F02_L03, mRNA sequence
Length = 668
Score = 90.0 bits (190), Expect(2) = 5e-025
Identities = 38/90 (42%), Positives = 51/90 (56%)
Frame = +1 / +1
Query: 196 QEQNSDLLLWRTGTLPAGLLTFYGLMEPLDRRWHVLGLGYDVDIDDRLIESAAVVHYNGN 375
Q + D + R LL+F L+ PLD R + GLGYD ID + S+A +HYNGN
Sbjct: 331 QVKIKDEVALREAAFSISLLSFQHLIYPLDERLILSGLGYDYGIDPEVARSSASLHYNGN 510
Query: 376 MKPWLKLAIRRYKYIWERYVNISHPYVREC 465
MKPWL+L I YK W R++ ++ EC
Sbjct: 511 MKPWLELGILEYKKYWRRFLTREDRFMDEC 600
Score = 38.6 bits (78), Expect(2) = 5e-025
Identities = 12/31 (38%), Positives = 18/31 (58%)
Frame = +1 / +1
Query: 94 SKIDPHTCGWAFGMNIFDLIAWRKANATSLY 186
+K DP +C W G+N+ +L W+K T Y
Sbjct: 223 TKYDPKSCAWKSGVNVINLDKWKKHKVTEKY 315
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,126,026
Number of Sequences: 8143
Number of extensions: 125067
Number of successful extensions: 1715
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 192400517
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)