TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3230260.2.1
(863 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN820523.1|CN820523 HRO4413_D02_G03ZS5 Lib AA069E1X Aven... 349 3e-097
gb|CN819665.1|CN819665 HRO4402_G05_M10ZS5 Lib AA069E1X Aven... 261 1e-070
gb|CN818757.1|CN818757 HRO4468_C07_E14ZS5 Lib AA070E1X Aven... 108 1e-027
gb|CN819392.1|CN819392 HRO4405_E01_I01ZS5 Lib AA069E1X Aven... 115 9e-027
>gb|CN820523.1|CN820523 HRO4413_D02_G03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4413_D02_G03, mRNA
sequence
Length = 704
Score = 349 bits (757), Expect = 3e-097
Identities = 142/209 (67%), Positives = 168/209 (80%)
Frame = -2 / +3
Query: 862 KACYEAKAKRXXXXXXXXXVFNNPDWPKGEAFTEDSWSDGEYCRKNEEWYFLSKTLAEHE 683
KACYEAK KR V+NNP+WPKG+ ED+WSD + CRKN++WY LSKTLAE E
Sbjct: 75 KACYEAKVKRVVVVSSVSAVYNNPNWPKGKVLDEDNWSDEDLCRKNKDWYDLSKTLAERE 254
Query: 682 AFAYAAKTGLDIVTICPSLVIGPLLQPAVNTSVKVFLSYIKGDQEIVKDGPKNLVDVRDV 503
AFAYAAKTGLDIVTICPSLVIGPLLQ VNTS KV LSY KG+ E V++ +NLVDVRDV
Sbjct: 255 AFAYAAKTGLDIVTICPSLVIGPLLQSTVNTSSKVLLSYFKGEHETVENKYRNLVDVRDV 434
Query: 502 AGALLLAYENLQASGRYLCSSPAIRVSDIVNILKTEYPTYTYPQKFVEAEGSNTYNTEKL 323
A A+LLAYEN +ASGRY+CSS I+ SD+++ILKT YPTY YP+ F E E S TY++EKL
Sbjct: 435 ADAILLAYENAEASGRYICSSSPIKASDLISILKTLYPTYPYPKNFAEVEHSTTYSSEKL 614
Query: 322 RKLGWTSRPMEDTLRDSIECYRAMGILN* 236
+KLGWT RP+E+TLRDS+E YRA G+LN*
Sbjct: 615 QKLGWTFRPIEETLRDSVESYRASGVLN* 701
Score = 219 bits (472), Expect = 6e-058
Identities = 113/210 (53%), Positives = 136/210 (64%)
Frame = -1 / +2
Query: 863 ESLL*GQSEESCCGIFSGGCVQ*S*LA*GRGLY*G*LVRWGILQKK*GMVFPF*NIGRT* 684
E LL G+S SC G+F CVQ*S*LA*G+ L * LVR G LQK+*G+V PF N R *
Sbjct: 74 EGLLRGKS*ASCRGVFCFCCVQ*S*LA*GQSLG*RQLVRRGPLQKE*GLV*PFQNACRA* 253
Query: 683 GFCLCSQNWARHCNYLPIIGNWTFVATCSKYKC*SLPQLYQRRSGDS*RWTQKSCGCS*C 504
GFCLCS+NW +CNYLPII NW A SK++ *S P L+ RR+ D + Q+ GCS C
Sbjct: 254 GFCLCSKNWTGYCNYLPIISNWPLAAVYSKHEQ*SPP*LF*RRTRDCRK*IQEPSGCSRC 433
Query: 503 RWCPSVGLRKSAGIRTIPLQFTSNKSL*YCKHTEDRISNIHLPSKIR*SGR*QHIQHGEA 324
C S G+ K G+ T+ +QF +N SL* KHTED ISN+ L K+ SG H+Q E
Sbjct: 434 CRCYSFGI*KCRGVWTLHMQFFTN*SL*SDKHTEDLISNVPLSQKLCRSGTQYHLQF*ET 613
Query: 323 SEARVDL*AHGGHPPRQHRMLQSYGHPELS 234
SE VDL AH G+P Q R+LQS PELS
Sbjct: 614 SETGVDLQAHRGNPQGQRRVLQSIWRPELS 703
Score = 188 bits (404), Expect = 1e-048
Identities = 105/209 (50%), Positives = 128/209 (61%)
Frame = -3 / +1
Query: 861 KLAMRPKRRELLWYLQWRLCSIILTGLRARPLLRIAGQMGNIAEKMRNGISFLKHWQNMR 682
+LA R K EL W L + LC+IILTGLRA+ ++ GQ AE++R G++F K Q++R
Sbjct: 76 RLATRQKLSELSWCLLFLLCTIILTGLRAKSWMKTTGQTRTSAERIRIGMTFPKRLQSVR 255
Query: 681 LLLMQPKLG*TL*LFAHHW*LDLCCNLQ*IQVLKSSSVISKEIRR*LKMDPKILWMFVMS 502
LLLMQ KL L*LFAHH *L CC+LQ* + +KSS VI KE R K++ WMF M
Sbjct: 256 LLLMQQKLDWIL*LFAHH**LAPCCSLQ*TRAVKSSLVILKENTRL*KINTGT*WMFEML 435
Query: 501 LVPFCWLTKICRHQDDTSAVHQQ*ESLIL*TY*RPNIQHTLTLKNSLKRKVATHTTRRSF 322
+ F W K+ R D T AV Q + LI * Y*RP IQ TL K K T R+F
Sbjct: 436 PMLFFWHMKMQRRLDATYAVLHQLKPLI**AY*RPYIQRTLIPKTLQKWNTVPLTVLRNF 615
Query: 321 GS*GGPLGPWRTPSETASNATELWAS*TE 235
+ GGP GP R PS TAS+ TE AS*TE
Sbjct: 616 RNWGGPSGP*RKPSGTASSPTEHLAS*TE 702
Score = 185 bits (398), Expect = 1e-047
Identities = 107/208 (51%), Positives = 119/208 (57%)
Frame = +1 / -3
Query: 235 LSSGCP*LCSIRCCLGGCPPWA*RSTLASEASPCCMCCYLPLQRIFEGKCMLDIRSSVCL 414
LSSG LC R C G P WA*RST SE S C +PL + F K LDIRSSVCL
Sbjct: 702 LSSGRQMLCRTRRCP*GFPLWA*RSTPVSEVSQNCKWYCVPLLQSFWDKGTLDIRSSVCL 523
Query: 415 QYQRLLLLVNCRGIVLMPADFRKPTEGHQRHHEHPQDFWVHL*LSPDLL*YN*GRL*HLY 594
QRL L+ NC V P F P E H++H EHP ++ S LL* N*G L
Sbjct: 522 SDQRL*LVKNCICSVQTPLHFHMPKE*HRQHLEHPLGSCIYFLQSRVLL*NN*GGLYCSC 343
Query: 595 LLQVATKVQLPMMGK*LQCLAQFWLHKQKPHVLPMF*KGNTIPHFFCNIPHLTSYPQ*RP 774
LL A + QL MMGK*LQ QF LHKQKPH L F KG+T P+ FC P LTS R
Sbjct: 342 LL*TAARGQLLMMGK*LQYPVQFLLHKQKPHALQAFWKGHTNPYSFCRGPRLTSCLHPRL 163
Query: 775 RP*ASQDY*TQPPLKIPQQLSSLWPHSK 858
P*AS DY TQ K P+QL+ L P SK
Sbjct: 162 CP*AS*DYCTQQKQKTPRQLA*LLPRSK 79
Score = 127 bits (272), Expect(2) = 4e-055
Identities = 61/97 (62%), Positives = 65/97 (67%)
Frame = +3 / -2
Query: 573 RKTLTLVFTAGCNKGPITNDGQIVTMSSPVLAA*AKASCSANVLERKYHSSFFLQYSPSD 752
R+TL LVFT C+KGPITNDGQIVT+SSPV AA*AKAS SA+VLER Y S FFLQ S SD
Sbjct: 364 RRTLLLVFTVDCSKGPITNDGQIVTISSPVFAA*AKASRSASVLERSYQSLFFLQRSSSD 185
Query: 753 QLSSVKASPLGQSGXXXXXXXXXXXXXXXXXXS*QAF 863
QLSS K PLGQ G S*QAF
Sbjct: 184 QLSSSKTLPLGQLGLLYTAETEDTTTTRLTFAS*QAF 74
Score = 123 bits (264), Expect(3) = 6e-039
Identities = 60/119 (50%), Positives = 74/119 (62%)
Frame = +2 / -1
Query: 353 FRFNEFLRVSVCWIFGLQYVYNIRDSYCW*TAEVSS*CLQIFVSQQKGTSDITNIHKIFG 532
F F + + V WI GLQY Y IR TA V+S L IF+ Q+ +I+NIH +
Sbjct: 584 FHFCKVFGIRVRWI*GLQYAYQIRGFNW*RTAYVASRRLCIFICQKNSIGNISNIH*VPV 405
Query: 533 SIFNYLLISFDITEEDFNTCIYCRLQQRSNYQ*WANSYNV*PSFGCISKSLMFCQCFRK 709
IF L+ SF IT+EDF +YCRLQQ +NY *WANSYN+ SF CISKSL C+ F K
Sbjct: 404 FIFYSLVFSFKITKEDFTARVYCRLQQGANY**WANSYNIQSSFCCISKSLTLCKRFGK 228
Score = 102 bits (218), Expect(2) = 4e-055
Identities = 55/99 (55%), Positives = 57/99 (57%)
Frame = +3 / -2
Query: 234 AQFRMPIAL*HSMLSRRVSSMGLEVHPSFRSFSVLYVLLPSASTNF*G*VYVGYSVFSMF 413
AQFR P AL* S LS RVSSMGL+VHPSF SFS L V+L S S F G* YVGY VFSM
Sbjct: 703 AQFRTPDAL*DSTLSLRVSSMGLKVHPSF*SFSEL*VVLCSTSAKFLG*GYVGYKVFSML 524
Query: 414 TISETLIAGELQRYRPDACRFXXXXXXXXXXXXXXXXFL 530
SE LI EL RPDA F FL
Sbjct: 523 IRSEALIGEELHM*RPDASAFSYAKRIASATSRTSTRFL 407
Score = 47.3 bits (97), Expect(3) = 6e-039
Identities = 20/28 (71%), Positives = 24/28 (85%)
Frame = +2 / -1
Query: 776 ALRPVRIIEHSRH*RYHNNSLRFGLIAS 859
ALRPVRII HSR+ R+H+NSL F L+AS
Sbjct: 161 ALRPVRIIVHSRNRRHHDNSLNFCLVAS 78
Score = 23.1 bits (44), Expect(3) = 6e-039
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = +2 / -1
Query: 239 VQDAHSSVAFDAVSE 283
VQDA SV DAV E
Sbjct: 698 VQDARCSVGLDAVPE 654
>gb|CN819665.1|CN819665 HRO4402_G05_M10ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4402_G05_M10, mRNA
sequence
Length = 632
Score = 261 bits (564), Expect(2) = 1e-070
Identities = 108/151 (71%), Positives = 127/151 (84%)
Frame = -2 / +2
Query: 688 HEAFAYAAKTGLDIVTICPSLVIGPLLQPAVNTSVKVFLSYIKGDQEIVKDGPKNLVDVR 509
HEAFAYAAKTGLDIVTICPSLVIGPLLQ VNTS KV LSY KG+ E V++ +NLVDVR
Sbjct: 5 HEAFAYAAKTGLDIVTICPSLVIGPLLQSTVNTSSKVLLSYFKGEHETVENKYRNLVDVR 184
Query: 508 DVAGALLLAYENLQASGRYLCSSPAIRVSDIVNILKTEYPTYTYPQKFVEAEGSNTYNTE 329
DVA A+LLAYEN +ASGRY+CSS I+ SD+++ILKT YPTY YP+ F E E S TY++E
Sbjct: 185 DVADAILLAYENAEASGRYICSSSPIKASDLISILKTLYPTYPYPKNFAEVEHSTTYSSE 364
Query: 328 KLRKLGWTSRPMEDTLRDSIECYRAMGILN* 236
KL+KLGWT RP+E+TLRDS+E YRA G+LN*
Sbjct: 365 KLQKLGWTFRPIEETLRDSVESYRASGVLN* 457
Score = 164 bits (352), Expect = 2e-041
Identities = 78/153 (50%), Positives = 96/153 (62%)
Frame = -1 / +1
Query: 683 GFCLCSQNWARHCNYLPIIGNWTFVATCSKYKC*SLPQLYQRRSGDS*RWTQKSCGCS*C 504
GFCLCS+NW +CNYLPII NW A SK++ *S P L+ RR+ D + Q+ GCS C
Sbjct: 10 GFCLCSKNWTGYCNYLPIISNWPLAAVYSKHEQ*SPP*LF*RRTRDCRK*IQEPSGCSRC 189
Query: 503 RWCPSVGLRKSAGIRTIPLQFTSNKSL*YCKHTEDRISNIHLPSKIR*SGR*QHIQHGEA 324
C S G+ K G+ T+ +QF +N SL* KHTED ISN+ L K+ SG H+Q E
Sbjct: 190 CRCYSFGI*KCRGVWTLHMQFFTN*SL*SDKHTEDLISNVPLSQKLCRSGTQYHLQF*ET 369
Query: 323 SEARVDL*AHGGHPPRQHRMLQSYGHPELSFPS 225
SE VDL AH G+P Q R+LQS PELS S
Sbjct: 370 SETGVDLQAHRGNPQGQRRVLQSIWRPELSIQS 468
Score = 131 bits (281), Expect = 1e-031
Identities = 77/150 (51%), Positives = 85/150 (56%)
Frame = +1 / -1
Query: 235 LSSGCP*LCSIRCCLGGCPPWA*RSTLASEASPCCMCCYLPLQRIFEGKCMLDIRSSVCL 414
LSSG LC R C G P WA*RST SE S C +PL + F K LDIRSSVCL
Sbjct: 458 LSSGRQMLCRTRRCP*GFPLWA*RSTPVSEVSQNCKWYCVPLLQSFWDKGTLDIRSSVCL 279
Query: 415 QYQRLLLLVNCRGIVLMPADFRKPTEGHQRHHEHPQDFWVHL*LSPDLL*YN*GRL*HLY 594
QRL L+ NC V P F P E H++H EHP ++ S LL* N*G L
Sbjct: 278 SDQRL*LVKNCICSVQTPLHFHMPKE*HRQHLEHPLGSCIYFLQSRVLL*NN*GGLYCSC 99
Query: 595 LLQVATKVQLPMMGK*LQCLAQFWLHKQKP 684
LL A + QL MMGK*LQ QF LHKQKP
Sbjct: 98 LL*TAARGQLLMMGK*LQYPVQFLLHKQKP 9
Score = 130 bits (279), Expect = 3e-031
Identities = 78/150 (52%), Positives = 89/150 (59%)
Frame = -3 / +3
Query: 684 RLLLMQPKLG*TL*LFAHHW*LDLCCNLQ*IQVLKSSSVISKEIRR*LKMDPKILWMFVM 505
RLLLMQ KL L*LFAHH *L CC+LQ* + +KSS VI KE R K++ WMF M
Sbjct: 9 RLLLMQQKLDWIL*LFAHH**LAPCCSLQ*TRAVKSSLVILKENTRL*KINTGT*WMFEM 188
Query: 504 SLVPFCWLTKICRHQDDTSAVHQQ*ESLIL*TY*RPNIQHTLTLKNSLKRKVATHTTRRS 325
+ F W K+ R D T AV Q + LI * Y*RP IQ TL K K T R+
Sbjct: 189 LPMLFFWHMKMQRRLDATYAVLHQLKPLI**AY*RPYIQRTLIPKTLQKWNTVPLTVLRN 368
Query: 324 FGS*GGPLGPWRTPSETASNATELWAS*TE 235
F + GGP GP R PS TAS+ TE AS*TE
Sbjct: 369 FRNWGGPSGP*RKPSGTASSPTEHLAS*TE 458
Score = 117 bits (250), Expect(2) = 2e-028
Identities = 57/112 (50%), Positives = 71/112 (63%)
Frame = +2 / -2
Query: 353 FRFNEFLRVSVCWIFGLQYVYNIRDSYCW*TAEVSS*CLQIFVSQQKGTSDITNIHKIFG 532
F F + + V WI GLQY Y IR TA V+S L IF+ Q+ +I+NIH +
Sbjct: 340 FHFCKVFGIRVRWI*GLQYAYQIRGFNW*RTAYVASRRLCIFICQKNSIGNISNIH*VPV 161
Query: 533 SIFNYLLISFDITEEDFNTCIYCRLQQRSNYQ*WANSYNV*PSFGCISKSLM 688
IF L+ SF IT+EDF +YCRLQQ +NY *WANSYN+ SF CISKSL+
Sbjct: 160 FIFYSLVFSFKITKEDFTARVYCRLQQGANY**WANSYNIQSSFCCISKSLV 5
Score = 103 bits (220), Expect(2) = 3e-040
Identities = 55/100 (55%), Positives = 58/100 (58%)
Frame = +3 / -3
Query: 231 EAQFRMPIAL*HSMLSRRVSSMGLEVHPSFRSFSVLYVLLPSASTNF*G*VYVGYSVFSM 410
+AQFR P AL* S LS RVSSMGL+VHPSF SFS L V+L S S F G* YVGY VFSM
Sbjct: 462 DAQFRTPDAL*DSTLSLRVSSMGLKVHPSF*SFSEL*VVLCSTSAKFLG*GYVGYKVFSM 283
Query: 411 FTISETLIAGELQRYRPDACRFXXXXXXXXXXXXXXXXFL 530
SE LI EL RPDA F FL
Sbjct: 282 LIRSEALIGEELHM*RPDASAFSYAKRIASATSRTSTRFL 163
Score = 76.7 bits (161), Expect(2) = 3e-040
Identities = 32/39 (82%), Positives = 35/39 (89%)
Frame = +3 / -3
Query: 573 RKTLTLVFTAGCNKGPITNDGQIVTMSSPVLAA*AKASC 689
R+TL LVFT C+KGPITNDGQIVT+SSPV AA*AKASC
Sbjct: 120 RRTLLLVFTVDCSKGPITNDGQIVTISSPVFAA*AKASC 4
Score = 23.1 bits (44), Expect(2) = 1e-070
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = -3 / +3
Query: 45 AN*LSTYICKKKKKK 1
+ *++ +CKKKKKK
Sbjct: 588 ST*INNELCKKKKKK 632
Score = 23.1 bits (44), Expect(2) = 2e-028
Identities = 10/15 (66%), Positives = 10/15 (66%)
Frame = +2 / -2
Query: 239 VQDAHSSVAFDAVSE 283
VQDA SV DAV E
Sbjct: 454 VQDARCSVGLDAVPE 410
>gb|CN818757.1|CN818757 HRO4468_C07_E14ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4468_C07_E14, mRNA sequence
Length = 805
Score = 108 bits (231), Expect(2) = 1e-027
Identities = 48/120 (40%), Positives = 70/120 (58%)
Frame = -2 / +3
Query: 601 AVNTSVKVFLSYIKGDQEIVKDGPKNLVDVRDVAGALLLAYENLQASGRYLCSSPAIRVS 422
AVNT+ V + +KG + + + +VDVRDVA ALLL Y +S RY+CS + +
Sbjct: 321 AVNTTSNVLIYILKGGPDTMNNKFYPMVDVRDVAEALLLLYNKAGSSERYICSLDQMDLK 500
Query: 421 DIVNILKTEYPTYTYPQKFVEAEGSNTYNTEKLRKLGWTSRPMEDTLRDSIECYRAMGIL 242
D++ ILK YP Y K V+ + ++KL+ LGW R +E+TL DSI+ Y G+L
Sbjct: 501 DLLGILKNMYPNYRCTDKMVDVDYKVIVTSDKLKNLGWKPRKLEETLEDSIKSYEKAGLL 680
Score = 29.5 bits (58), Expect(2) = 1e-027
Identities = 17/50 (34%), Positives = 24/50 (48%)
Frame = -3 / +1
Query: 861 KLAMRPKRRELLWYLQWRLCSIILTGLRARPLLRIAGQMGNIAEKMRNGI 712
+LA K + W L ++ GLR + R AGQ + A +MR GI
Sbjct: 58 RLAQLRK*KNSSWSRL*PLVALRWIGLRIKSKTRAAGQTKSYARRMRTGI 207
>gb|CN819392.1|CN819392 HRO4405_E01_I01ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4405_E01_I01, mRNA
sequence
Length = 717
Score = 115 bits (246), Expect = 9e-027
Identities = 52/125 (41%), Positives = 72/125 (57%)
Frame = -2 / +1
Query: 763 EDSWSDGEYCRKNEEWYFLSKTLAEHEAFAYAAKTGLDIVTICPSLVIGPLLQPAVNTSV 584
E +S E C K ++WY LSKTLAE A+ ++ +IVTI P++VIGPLLQP +NTS
Sbjct: 289 ETWFSHAELCEKGQQWYVLSKTLAEEAAWKFSKDNAFEIVTINPAMVIGPLLQPTLNTSA 468
Query: 583 KVFLSYIKGDQEIVKDGPKNLVDVRDVAGALLLAYENLQASGRYLCSSPAIRVSDIVNIL 404
+ L I G + ++V+DVA A +LAYE A+GRY S++VN +
Sbjct: 469 EAILKLINGSSSTYPNFSFGWINVKDVALAHILAYEVPSANGRYCMVERVAHHSELVNFI 648
Query: 403 KTEYP 389
YP
Sbjct: 649 HKMYP 663
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,678,401
Number of Sequences: 8143
Number of extensions: 140843
Number of successful extensions: 1289
Number of sequences better than 1.0e-020: 4
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 297030472
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)