TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3198841.2.2
(602 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN816396.1|CN816396 HRO4520_C05_F10ZS5 Lib AA071E1X Aven... 279 2e-077
>gb|CN816396.1|CN816396 HRO4520_C05_F10ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_C05_F10, mRNA sequence
Length = 851
Score = 279 bits (603), Expect(2) = 2e-077
Identities = 101/133 (75%), Positives = 114/133 (85%)
Frame = +3 / +2
Query: 174 INGSNPDDYEKFEKGIMHYGCPHYRRRCRIRAPCCNEIFDCRHCHNETKNSIKIDKMKRH 353
INGS+PDDYE+ EKG+M YGC HYRRRCRIRAPCCNEIFDCRHCHNE+KNSIK D ++RH
Sbjct: 29 INGSSPDDYERLEKGLMQYGCAHYRRRCRIRAPCCNEIFDCRHCHNESKNSIKTDVIRRH 208
Query: 354 ELPRHEVQQVVCSLCGTEQEVRQVCINCGVCMGKYFCGLCKLFDDDVSKQQYHCNXXXXX 533
EL RHEVQQV+CSLCGTEQEV ++CI+CGVCMGKYFC +CKLFDDD+SKQQYHC+
Sbjct: 209 ELSRHEVQQVICSLCGTEQEVGKICISCGVCMGKYFCEVCKLFDDDISKQQYHCHGCGIC 388
Query: 534 XXXXXENSFHCSK 572
EN FHCSK
Sbjct: 389 RIGGGENFFHCSK 427
Score = 204 bits (439), Expect = 1e-053
Identities = 85/114 (74%), Positives = 90/114 (78%)
Frame = -2 / -3
Query: 514 Q*YCCLETSSSKSLHKPQKYFPMQTPQLIHTCRTSCSVPHNEHTTCCTSWRGSSCLFILS 335
Q*YCC E SSSKSLH QKYFP+ TPQL+H TSCSVPHNEH TCCTS R SSC I S
Sbjct: 369 Q*YCCFEISSSKSLHTSQKYFPIHTPQLMHIFPTSCSVPHNEHMTCCTS*RESSCRLITS 190
Query: 334 ILMEFLVSLWQCRQSKISLQQGALMRHLLL*CGHP*CIIPFSNFS*SSGFEPLI 173
+L+EF SLWQCRQSKISLQ GA MRHLL *C HP CI PFS+ S*SSG EPLI
Sbjct: 189 VLIEFFDSLWQCRQSKISLQHGARMRHLLR*CAHPYCINPFSSLS*SSGLEPLI 28
Score = 168 bits (361), Expect = 8e-043
Identities = 79/133 (59%), Positives = 96/133 (72%)
Frame = -1 / -2
Query: 572 F*TVEGILPASDSTYSTSVAVILLFRDIIVEEFAQTTEVLPHANTAIDTYLSHLLFSATQ 393
F* V+ IL A++STYST++ VILLF DII++EFA TE+L H T D Y +LLFS T
Sbjct: 427 F*AVKEILAATNSTYSTTMTVILLF*DIIIKEFAHFTEILSHTYTTADAYFPYLLFSTT* 248
Query: 392 *AYNLLHFMAWKFMPLHFINFNGILSFIVAVSAIKNFIAARSSYAASPSIMWTPIVHYPF 213
*AY+LLHFM KFMP + I+FN IL FIVAV IKN +AA SYAA PS+M TPI+H
Sbjct: 247 *AYDLLHFMT*KFMPPNHISFNRIL*FIVAVPTIKNLVAAWCSYAAPPSVMCTPILHQSL 68
Query: 212 LKFLIVIRV*TID 174
L+ LI+IR ID
Sbjct: 67 LQPLIIIRARAID 29
Score = 163 bits (351), Expect = 2e-041
Identities = 77/134 (57%), Positives = 91/134 (67%)
Frame = -3 / -1
Query: 573 LLNSGRNSPCLRFYIFHIRCSDTAV*RHHRRRVCTNHRSTSPCKHRN*YILVAPPVQCHT 394
+L+S RNS +FYIFH SDTAV R+H +RVCT HR+T P H +* I P VQ H
Sbjct: 428 ILSSERNSRRHQFYIFHNHDSDTAVLRYHHQRVCTLHRNTFPYIHHS*CIFSLPLVQYHI 249
Query: 393 MSIQPAALHGVEVHASSFYQF*WNS*FHCGSVGNQKFHCSKELLCGISFYNVDTHSALSL 214
MSI PAALH V+VHA+ +QF* NS HCGS NQK CS L+CG SF NV TH+A
Sbjct: 248 MSI*PAALHDVKVHAA*SHQF*SNSLIHCGSADNQKSRCSMVLVCGTSFGNVHTHTASIP 69
Query: 213 SQISHSHQGLNH*S 172
S SH+HQG +H*S
Sbjct: 68 SPASHNHQG*SH*S 27
Score = 161 bits (347), Expect = 7e-041
Identities = 79/132 (59%), Positives = 86/132 (65%)
Frame = +1 / +3
Query: 175 SMVQTLMTMRNLRKG*CTMGVHIIEGDAA*ELLAAMKFLIADTATMKLRIPLKLIK*RGM 354
SM LM MR R+ *C+MGVHI EG AA E AA +FLI TATM RI LKL+ GM
Sbjct: 30 SMALALMIMRGWRRD*CSMGVHITEGGAAYEHHAATRFLIVGTATMNQRIRLKLM*LGGM 209
Query: 355 NFHAMKCSRLYAHCVALNRRCDKYVSIAVFAWGSTSVVCANSSTMMSLNSSITATDVEYV 534
NFH MKCSR YAH V LN+R KY S V+ W S SV CANS M+S NSSIT VEYV
Sbjct: 210 NFHVMKCSRSYAHYVVLNKR*GKYASAVVYVWESISVKCANSLMMISQNSSITVMVVEYV 389
Query: 535 ESEAGRIPSTVQ 570
E A RI T Q
Sbjct: 390 ELVAARISFTAQ 425
Score = 139 bits (298), Expect = 4e-034
Identities = 78/133 (58%), Positives = 94/133 (70%)
Frame = +2 / +1
Query: 173 DQWFKP**L*EI*ERDNALWVSTL*KEMPHKSSLLQ*NF*LPTLPQ*N*EFH*N**NEEA 352
DQW P**L*E E +A+WV TL KE+PH S++LQ +F*L LPQ* EF *N* + A
Sbjct: 28 DQWL*P**L*EAGEGIDAVWVCTLPKEVPHTSTMLQRDF*LSALPQ*IKEFD*N*CD*AA 207
Query: 353 *TSTP*SAAGCMLIVWH*TGGATSMYQLRCLHGEVLLWFVQTLRR*CL*TAVSLQRMWNM 532
*T T *SAAG MLI+W+*T G +M+QL C++G+V L VQTL * L TAVSL +WNM
Sbjct: 208 *TFTS*SAAGHMLIMWY*TRGRENMHQLWCMYGKVFL*SVQTL***YLKTAVSLSWLWNM 387
Query: 533 *NRRQGEFLPLFK 571
*N + EFL L K
Sbjct: 388 *NWWRREFLSLLK 426
Score = 24.9 bits (48), Expect(2) = 2e-077
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +2 / +2
Query: 554 FLPLFKSGCCYSIALK 601
F K GCCYS LK
Sbjct: 410 FFHCSKCGCCYSTVLK 457
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,398,222
Number of Sequences: 8143
Number of extensions: 120029
Number of successful extensions: 1748
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 193625998
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)