TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3184927.2.1
(1157 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819392.1|CN819392 HRO4405_E01_I01ZS5 Lib AA069E1X Aven... 64 4e-043
gb|CN820523.1|CN820523 HRO4413_D02_G03ZS5 Lib AA069E1X Aven... 60 2e-024
>gb|CN819392.1|CN819392 HRO4405_E01_I01ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4405_E01_I01, mRNA
sequence
Length = 717
Score = 64.3 bits (134), Expect(4) = 4e-043
Identities = 30/101 (29%), Positives = 50/101 (49%)
Frame = -1 / +1
Query: 779 VLTSSSSAVRIRDDGGQSSNISLDETTWSSVPLCEKMHLWYALXXXXXXXXXXXXXXENG 600
V+ +SS A + ++ N+ +DET +S LCEK WY L +N
Sbjct: 217 VIVTSSMAAVGYNGKPKTPNVVVDETWFSHAELCEKGQQWYVLSKTLAEEAAWKFSKDNA 396
Query: 599 IDLVTVLPSFVIGPSLSHELCVTASDVLGLFQGDTARFSSY 477
++VT+ P+ VIGP L L +A +L L G ++ + ++
Sbjct: 397 FEIVTINPAMVIGPLLQPTLNTSAEAILKLINGSSSTYPNF 519
Score = 62.0 bits (129), Expect(4) = 4e-043
Identities = 25/37 (67%), Positives = 29/37 (78%)
Frame = -1 / +1
Query: 1001 LPGAKERLQIVRADLLEEGSFDSAVMACEGVFHTASP 891
L GAK+RL + +A LLEEGSFD A+ CE VFHTASP
Sbjct: 4 LDGAKDRLHLFKASLLEEGSFDDAIDGCECVFHTASP 114
Score = 56.1 bits (116), Expect(4) = 4e-043
Identities = 22/53 (41%), Positives = 32/53 (60%)
Frame = -1 / +1
Query: 467 GYVHIDDVASSHILVYEAPQAAGRYLCSSVVLDNDELVSSLAKRYPIFPIPRR 309
G++++ DVA +HIL YE P A GRY V + ELV+ + K YP P+ +
Sbjct: 526 GWINVKDVALAHILAYEVPSANGRYCMVERVAHHSELVNFIHKMYPDIPVAHK 684
Score = 44.1 bits (90), Expect(4) = 4e-043
Identities = 19/35 (54%), Positives = 25/35 (71%)
Frame = -1 / +1
Query: 863 KEDTLVPAVNGTLNVLRSCKKNPFLKRVVLTSSSS 759
K + L PAVNGTLNVLRSCKK + +V +S ++
Sbjct: 139 KAELLDPAVNGTLNVLRSCKKASIKRVIVTSSMAA 243
>gb|CN820523.1|CN820523 HRO4413_D02_G03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4413_D02_G03, mRNA
sequence
Length = 704
Score = 59.7 bits (124), Expect(3) = 2e-024
Identities = 25/72 (34%), Positives = 35/72 (48%)
Frame = -1 / +3
Query: 713 LDETTWSSVPLCEKMHLWYALXXXXXXXXXXXXXXENGIDLVTVLPSFVIGPSLSHELCV 534
LDE WS LC K WY L + G+D+VT+ PS VIGP L +
Sbjct: 168 LDEDNWSDEDLCRKNKDWYDLSKTLAEREAFAYAAKTGLDIVTICPSLVIGPLLQSTVNT 347
Query: 533 TASDVLGLFQGD 498
++ +L F+G+
Sbjct: 348 SSKVLLSYFKGE 383
Score = 54.7 bits (113), Expect(3) = 2e-024
Identities = 24/74 (32%), Positives = 38/74 (51%)
Frame = -1 / +3
Query: 461 VHIDDVASSHILVYEAPQAAGRYLCSSVVLDNDELVSSLAKRYPIFPIPRRLNSPYGKQS 282
V + DVA + +L YE +A+GRY+CSS + +L+S L YP +P P+ +
Sbjct: 417 VDVRDVADAILLAYENAEASGRYICSSSPIKASDLISILKTLYPTYPYPKNFAEVEHSTT 596
Query: 281 YQLNTSKLQGLGFK 240
Y + G F+
Sbjct: 597 YSSEKLQKLGWTFR 638
Score = 30.9 bits (61), Expect(3) = 2e-024
Identities = 13/31 (41%), Positives = 21/31 (67%)
Frame = -1 / +3
Query: 851 LVPAVNGTLNVLRSCKKNPFLKRVVLTSSSS 759
+ PAV GTLN L++C + + VV++S S+
Sbjct: 39 IAPAVTGTLNELKACYEAKVKRVVVVSSVSA 131
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,295,987
Number of Sequences: 8143
Number of extensions: 194558
Number of successful extensions: 1404
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 412335495
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)