TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3115139.2.1
(1079 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819392.1|CN819392 HRO4405_E01_I01ZS5 Lib AA069E1X Aven... 89 2e-048
gb|CN820523.1|CN820523 HRO4413_D02_G03ZS5 Lib AA069E1X Aven... 73 1e-025
gb|CN818757.1|CN818757 HRO4468_C07_E14ZS5 Lib AA070E1X Aven... 68 4e-025
>gb|CN819392.1|CN819392 HRO4405_E01_I01ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4405_E01_I01, mRNA
sequence
Length = 717
Score = 89.0 bits (188), Expect(4) = 2e-048
Identities = 33/83 (39%), Positives = 53/83 (63%)
Frame = +1 / +1
Query: 424 PNVLLDESSWSSIEFCESLQIWYAVAKILAEKAAWEFAGEHRIDLVTVLPTFVVGPTLSP 603
PNV++DE+ +S E CE Q WY ++K LAE+AAW+F+ ++ ++VT+ P V+GP L P
Sbjct: 271 PNVVVDETWFSHAELCEKGQQWYVLSKTLAEEAAWKFSKDNAFEIVTINPAMVIGPLLQP 450
Query: 604 ELGPTASDVLGLFQGETGKFTTY 672
L +A +L L G + + +
Sbjct: 451 TLNTSAEAILKLINGSSSTYPNF 519
Score = 67.0 bits (140), Expect(4) = 2e-048
Identities = 27/37 (72%), Positives = 31/37 (83%)
Frame = +1 / +1
Query: 154 LEGAKERLQLVRADLLEEGSFDDAVMACEGVFHTASP 264
L+GAK+RL L +A LLEEGSFDDA+ CE VFHTASP
Sbjct: 4 LDGAKDRLHLFKASLLEEGSFDDAIDGCECVFHTASP 114
Score = 45.1 bits (92), Expect(4) = 2e-048
Identities = 18/50 (36%), Positives = 27/50 (54%)
Frame = +1 / +1
Query: 682 GYVHIDDVARCHMLAYEAAGARGRYICSAAVLDCGDLAALLARRFPAYPV 831
G++++ DVA H+LAYE A GRY V +L + + +P PV
Sbjct: 526 GWINVKDVALAHILAYEVPSANGRYCMVERVAHHSELVNFIHKMYPDIPV 675
Score = 43.2 bits (88), Expect(4) = 2e-048
Identities = 16/21 (76%), Positives = 19/21 (90%)
Frame = +1 / +1
Query: 286 KEEMLDSAINGTMNVLRSCKK 348
K E+LD A+NGT+NVLRSCKK
Sbjct: 139 KAELLDPAVNGTLNVLRSCKK 201
>gb|CN820523.1|CN820523 HRO4413_D02_G03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4413_D02_G03, mRNA
sequence
Length = 704
Score = 73.5 bits (154), Expect(2) = 1e-025
Identities = 27/77 (35%), Positives = 47/77 (61%)
Frame = +1 / +3
Query: 421 PPNVLLDESSWSSIEFCESLQIWYAVAKILAEKAAWEFAGEHRIDLVTVLPTFVVGPTLS 600
P +LDE +WS + C + WY ++K LAE+ A+ +A + +D+VT+ P+ V+GP L
Sbjct: 153 PKGKVLDEDNWSDEDLCRKNKDWYDLSKTLAEREAFAYAAKTGLDIVTICPSLVIGPLLQ 332
Query: 601 PELGPTASDVLGLFQGE 651
+ ++ +L F+GE
Sbjct: 333 STVNTSSKVLLSYFKGE 383
Score = 58.3 bits (121), Expect(2) = 1e-025
Identities = 24/61 (39%), Positives = 35/61 (57%)
Frame = +1 / +3
Query: 688 VHIDDVARCHMLAYEAAGARGRYICSAAVLDCGDLAALLARRFPAYPVPRSLPRAYGEQS 867
V + DVA +LAYE A A GRYICS++ + DL ++L +P YP P++ +
Sbjct: 417 VDVRDVADAILLAYENAEASGRYICSSSPIKASDLISILKTLYPTYPYPKNFAEVEHSTT 596
Query: 868 Y 870
Y
Sbjct: 597 Y 599
>gb|CN818757.1|CN818757 HRO4468_C07_E14ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4468_C07_E14, mRNA sequence
Length = 805
Score = 67.5 bits (141), Expect(3) = 4e-025
Identities = 29/73 (39%), Positives = 43/73 (58%)
Frame = +1 / +3
Query: 415 DLPPNVLLDESSWSSIEFCESLQIWYAVAKILAEKAAWEFAGEHRIDLVTVLPTFVVGPT 594
D PP+ + DES WS E C+ + WY++AK AE+ A E+ ++ + + T+LP V GP
Sbjct: 129 DWPPDKIKDESCWSDKELCKENENWYSLAKTTAEEMALEYGLKNGLHIATLLPGLVFGPL 308
Query: 595 LSPELGPTASDVL 633
L T S+VL
Sbjct: 309 LQHVAVNTTSNVL 347
Score = 44.6 bits (91), Expect(3) = 4e-025
Identities = 19/46 (41%), Positives = 25/46 (54%)
Frame = +1 / +3
Query: 688 VHIDDVARCHMLAYEAAGARGRYICSAAVLDCGDLAALLARRFPAY 825
V + DVA +L Y AG+ RYICS +D DL +L +P Y
Sbjct: 402 VDVRDVAEALLLLYNKAGSSERYICSLDQMDLKDLLGILKNMYPNY 539
Score = 35.4 bits (71), Expect(3) = 4e-025
Identities = 13/20 (65%), Positives = 16/20 (80%)
Frame = +1 / +3
Query: 283 SKEEMLDSAINGTMNVLRSC 342
S E MLD A+ GTMNVL++C
Sbjct: 6 STERMLDPAVKGTMNVLKAC 65
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,710,477
Number of Sequences: 8143
Number of extensions: 152733
Number of successful extensions: 1627
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 380896425
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)