TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3071418.2.1
(1432 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815034.1|CN815034 HRO4505_B06_C11ZS5 Lib AA071E1X Aven... 164 3e-041
gb|CN816101.1|CN816101 HRO4517_A05_A09ZS5 Lib AA071E1X Aven... 160 7e-040
gb|CN816873.1|CN816873 HRO4525_G05_M09ZS5 Lib AA071E1X Aven... 138 3e-036
gb|CN815850.1|CN815850 HRO4511_B09_D17ZS5 Lib AA071E1X Aven... 137 5e-033
gb|CN814875.1|CN814875 HRO4503_D10_H19ZS5 Lib AA071E1X Aven... 136 7e-033
gb|CN821053.1|CN821053 HRO4427_A04_B07ZS5 Lib AA069E1X Aven... 135 1e-032
gb|CN814789.1|CN814789 HRO4502_E05_I10ZS5 Lib AA071E1X Aven... 112 1e-025
>gb|CN815034.1|CN815034 HRO4505_B06_C11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4505_B06_C11, mRNA sequence
Length = 629
Score = 164 bits (353), Expect = 3e-041
Identities = 63/133 (47%), Positives = 90/133 (67%)
Frame = -1 / +1
Query: 643 PHLKCSVLDLPHVVAGAPSDGNVQFVAGNMFESIPPATAVFLKKTLHDWGDDECVKILKN 464
PH+KC+VLDLP V+ PSDG V +VAG++F S+P + AV LK LH W DD+CVKIL
Sbjct: 58 PHIKCTVLDLPKVIEKVPSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDDDCVKILSQ 237
Query: 463 CKQAISPRDAGGKVIILDVVVGYKQSNIKHQETQVMFDLYMMAVNGVERDEQEWKKIFTE 284
C+ AI R+ GGKVII+D++VG I + +M ++ G +RDE +W+++F +
Sbjct: 238 CRSAIPSREEGGKVIIIDILVGPSLGPIMFEAQILMDMAMLVNTRGRQRDENDWRQLFMK 417
Query: 283 AGFKDYKILPVIG 245
AGF DYK++ +G
Sbjct: 418 AGFSDYKVVKKLG 456
>gb|CN816101.1|CN816101 HRO4517_A05_A09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_A05_A09, mRNA sequence
Length = 652
Score = 160 bits (343), Expect = 7e-040
Identities = 64/139 (46%), Positives = 93/139 (66%)
Frame = -1 / +3
Query: 646 FPHLKCSVLDLPHVVAGAPSDGNVQFVAGNMFESIPPATAVFLKKTLHDWGDDECVKILK 467
+PHLKC+VLDLP V+ AP + +VAG++F ++PP+ AV LK LH W D++CVKIL
Sbjct: 21 YPHLKCTVLDLPKVIEKAPPHDVINYVAGDLFHTVPPSQAVMLKLVLHFWSDEDCVKILA 200
Query: 466 NCKQAISPRDAGGKVIILDVVVGYKQSNIKHQETQVMFDLYMMAVNGVERDEQEWKKIFT 287
C++AI R+ GGKVII+++VV I + + L M+ G +RDE+ W+++F
Sbjct: 201 QCRKAIPSREEGGKVIIIEIVVEPSLGPIMFEAQLLFDMLMMVNTRGGQRDEKHWRELFM 380
Query: 286 EAGFKDYKILPVIGDVSVI 230
+AGF DYKI+ +G SVI
Sbjct: 381 QAGFTDYKIVKKLGARSVI 437
>gb|CN816873.1|CN816873 HRO4525_G05_M09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4525_G05_M09, mRNA sequence
Length = 662
Score = 138 bits (297), Expect(2) = 3e-036
Identities = 54/121 (44%), Positives = 79/121 (65%)
Frame = -1 / +2
Query: 607 VVAGAPSDGNVQFVAGNMFESIPPATAVFLKKTLHDWGDDECVKILKNCKQAISPRDAGG 428
V+ PSDG V +VAG++F S+P + AV LK LH W DD+CVKIL C+ AI R+ GG
Sbjct: 41 VIEKVPSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDDDCVKILSQCRSAIPSREEGG 220
Query: 427 KVIILDVVVGYKQSNIKHQETQVMFDLYMMAVNGVERDEQEWKKIFTEAGFKDYKILPVI 248
KVII+D++VG I + +M ++ G +RDE +W+++F +AGF DYK++ +
Sbjct: 221 KVIIIDILVGPSLGPIMFEAQILMDMAMLVNTRGRQRDENDWRQLFMKAGFSDYKVVKKL 400
Query: 247 G 245
G
Sbjct: 401 G 403
Score = 29.0 bits (57), Expect(2) = 3e-036
Identities = 9/11 (81%), Positives = 11/11 (100%)
Frame = -1 / +3
Query: 643 PHLKCSVLDLP 611
PH+KC+VLDLP
Sbjct: 6 PHIKCTVLDLP 38
>gb|CN815850.1|CN815850 HRO4511_B09_D17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_B09_D17, mRNA sequence
Length = 621
Score = 137 bits (293), Expect = 5e-033
Identities = 54/116 (46%), Positives = 77/116 (66%)
Frame = -1 / +2
Query: 592 PSDGNVQFVAGNMFESIPPATAVFLKKTLHDWGDDECVKILKNCKQAISPRDAGGKVIIL 413
PSDG V +VAG++F S+P + AV LK LH W D++CVKIL CK AI R+ GGKVII+
Sbjct: 2 PSDGIVYYVAGDLFHSVPKSQAVMLKLVLHRWSDEDCVKILSQCKSAIPSREEGGKVIII 181
Query: 412 DVVVGYKQSNIKHQETQVMFDLYMMAVNGVERDEQEWKKIFTEAGFKDYKILPVIG 245
D++VG I + +M ++ G +RDE +W+++F +AGF DYKI+ +G
Sbjct: 182 DILVGPSLGPIMFEAQVLMDMAMLVNTRGRQRDENDWRQLFMKAGFSDYKIVKKVG 349
>gb|CN814875.1|CN814875 HRO4503_D10_H19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_D10_H19, mRNA sequence
Length = 846
Score = 136 bits (292), Expect = 7e-033
Identities = 56/89 (62%), Positives = 72/89 (80%)
Frame = -1 / +1
Query: 496 GDDECVKILKNCKQAISPRDAGGKVIILDVVVGYKQSNIKHQETQVMFDLYMMAVNGVER 317
GD++CVKILK CK+AI RDAGGKVII+D+VVG ++I +ETQV FDL++M + G+ER
Sbjct: 10 GDEDCVKILKKCKEAIPSRDAGGKVIIVDMVVGSGPNDIVTRETQVFFDLFIMYLEGIER 189
Query: 316 DEQEWKKIFTEAGFKDYKILPVIGDVSVI 230
+E EWKKIF EAGF +YKI+ V+G SVI
Sbjct: 190 EEFEWKKIFMEAGFSEYKIISVLGVRSVI 276
>gb|CN821053.1|CN821053 HRO4427_A04_B07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_A04_B07, mRNA
sequence
Length = 644
Score = 135 bits (290), Expect = 1e-032
Identities = 53/116 (45%), Positives = 77/116 (66%)
Frame = -1 / +3
Query: 592 PSDGNVQFVAGNMFESIPPATAVFLKKTLHDWGDDECVKILKNCKQAISPRDAGGKVIIL 413
PSDG V +VAG++F S+P + AV LK LH W D++CVKIL C+ AI R+ GGKVII+
Sbjct: 24 PSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDEDCVKILSQCRNAIPSREKGGKVIII 203
Query: 412 DVVVGYKQSNIKHQETQVMFDLYMMAVNGVERDEQEWKKIFTEAGFKDYKILPVIG 245
D++VG I + +M ++ G +RDE +W+++F +AGF DYKI+ +G
Sbjct: 204 DILVGPSLGPIMFEAQILMDMAMLVNTRGRQRDENDWRQLFMKAGFSDYKIVKKLG 371
>gb|CN814789.1|CN814789 HRO4502_E05_I10ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_E05_I10, mRNA sequence
Length = 596
Score = 112 bits (240), Expect = 1e-025
Identities = 44/101 (43%), Positives = 65/101 (64%)
Frame = -1 / -3
Query: 547 SIPPATAVFLKKTLHDWGDDECVKILKNCKQAISPRDAGGKVIILDVVVGYKQSNIKHQE 368
S+P + AV LK LH W D++CVKIL C+ AI R+ GGKVII+D++VG I +
Sbjct: 591 SVPKSQAVLLKLVLHHWSDEDCVKILSQCRNAIPSREKGGKVIIIDILVGPSLGPIMFEA 412
Query: 367 TQVMFDLYMMAVNGVERDEQEWKKIFTEAGFKDYKILPVIG 245
+M ++ G +RDE +W+++F +AGF DYKI+ +G
Sbjct: 411 QILMDMAMLVNTRGRQRDENDWRQLFMKAGFSDYKIVKKLG 289
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 15,722,456
Number of Sequences: 8143
Number of extensions: 219803
Number of successful extensions: 1526
Number of sequences better than 1.0e-020: 7
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 522372240
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)