TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3070863.2.1
(992 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN814741.1|CN814741 HRO4502_A04_A08ZS5 Lib AA071E1X Aven... 302 8e-083
>gb|CN814741.1|CN814741 HRO4502_A04_A08ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_A04_A08, mRNA sequence
Length = 724
Score = 302 bits (653), Expect = 8e-083
Identities = 132/176 (75%), Positives = 138/176 (78%)
Frame = +1 / +2
Query: 301 KSFGHIDILVSNAAANPSVDSILEMKESVLDKLWDINVKASILLIQDAAPHLRKGXXXXX 480
+ FG IDI+VSNAAANPSV ILEMKE VLDKLWDINVKASILL+QDAAPHLR G
Sbjct: 11 QEFGQIDIVVSNAAANPSVQGILEMKEPVLDKLWDINVKASILLLQDAAPHLRNGSSVII 190
Query: 481 XXXXAGYNPEQGLTMYGVTKTALFGLTKALAGEMGPDTRVNCIAPGFVPTRFASFLTENE 660
AGYNPE L MYGVTKTALFGLTKALA EMGP+TRVNCIAPGFVPTRFA FLT NE
Sbjct: 191 ISSIAGYNPEATLGMYGVTKTALFGLTKALATEMGPNTRVNCIAPGFVPTRFAGFLTTNE 370
Query: 661 TIRKELNERTKLKRLGTVEDMXXXXXXXXXXXXXYITAETIVVAGGVQSRL*IWLK 828
T+R EL ER+ LKRLGTVEDM YITAETIVVAGG QSRL*IWLK
Sbjct: 371 TLRNELMERSTLKRLGTVEDMASAAAFLASDDASYITAETIVVAGGTQSRL*IWLK 538
Score = 247 bits (533), Expect = 3e-066
Identities = 98/176 (55%), Positives = 123/176 (69%)
Frame = +3 / +1
Query: 306 LWAHRYSCLQCCRKSFCR*HT*NERVCSR*AVGY*RQGFYPSYSGCCSSPTEGVICDYYF 485
+W Y CLQCC KSFC HT +ER S A+GY*RQ Y S SGCC++ E ++ D F
Sbjct: 16 IWTD*YCCLQCCCKSFCTRHTRDERASS*QAMGY*RQSLYSSTSGCCTTFEERIVRDNNF 195
Query: 486 FNCWLQSRTRIDNVWCHKDCSLWSHEGSCW*DGTRYSC*LYSPWFCSYTVC*FPHRK*DH 665
FNCWL S + +VWC+KDC +WSH+GSC+*DG +Y C*LYS WFCSYT+C FPH *D
Sbjct: 196 FNCWL*S*SNFRDVWCYKDCPVWSHKGSCY*DGPKYPC*LYSSWFCSYTLCWFPHD**DP 375
Query: 666 *ERA*REDQA*EIGYCGRHGCGCGFSGV*RRIIHYG*NHCCCWRGAV*AVNLAEVY 833
*+ A E+ *EIGYC RHG G+ RRI+HY *N+CCC R +* +NLA+++
Sbjct: 376 *K*AHGEEHT*EIGYC*RHGFSRRLPGIGRRILHYS*NNCCCRRNPI*TINLAKIW 543
Score = 228 bits (493), Expect = 9e-061
Identities = 113/174 (64%), Positives = 116/174 (66%)
Frame = -1 / -2
Query: 830 NFSQIYSLDCTPPATTMVSAVMYDXXXXXXXXXXXXXXSTVPNXXXXXXXXXXXXXXXXX 651
+FSQIYSLD PPATT+VSAVM D STVPN
Sbjct: 540 DFSQIYSLDWVPPATTIVSAVM*DASSDARKAAAEAMSSTVPNLLSVLLSMSSFLRVSLV 361
Query: 650 VRKLANRVGTKPGAIQLTRVSGPISPARAFVRPKRAVFVTPYIVNPCSGL*PAIEEIIIT 471
VRK A RVGTKPGAIQLTRV GPIS ARAFVRP RAVFVTPYI SGL*PAIEEIIIT
Sbjct: 360 VRKPAKRVGTKPGAIQLTRVFGPISVARAFVRPNRAVFVTPYIPKVASGL*PAIEEIIIT 181
Query: 470 DDPFXXXXXXXXXXXIEALTLISHSLSRTDSFISSMLSTEGFAAALETRISMCP 309
DDPF IEALTLISHSLSRT SFISSM TEGFAAALET IS+CP
Sbjct: 180 DDPFLKCGAAS*SRRIEALTLISHSLSRTGSFISSMPCTEGFAAALETTISICP 19
Score = 213 bits (459), Expect = 5e-056
Identities = 102/174 (58%), Positives = 118/174 (67%)
Frame = -3 / -1
Query: 822 PDLQPRLHPSSNNNGFSRNV*CVVRRQKSRSRSHVFHSTQSLKLGPLVKLFPNGLIFCEE 643
PDL RL S NNN FS NV CVVR Q+ SHVF+STQSLK PL +L GLI EE
Sbjct: 532 PDL*SRLGSSGNNNCFSCNVGCVVRCQEGGG*SHVFNSTQSLKCAPLHELISKGLISREE 353
Query: 642 TSKPCRNKTRGYTVNTSIGSHLTSKSLRETKESSLCDTIHCQSLFWIVTSXXXXXXXX*P 463
TSK CRNKTR YTVNT I +HL+SKSL ETK+ SLC+TIH S F I+TS
Sbjct: 352 TSKACRNKTRSYTVNTGIWAHLSSKSLCETKQGSLCNTIHP*SCFRIITSN*RNYYHGRS 173
Query: 462 LP*VRSSILNKKDRSLDVNIPQLIENRLFHFKYAIYRRICGSIGDKNIYVPKAL 301
+P + SIL K+R DVNIP L++N LFH +YA+YRRIC SIGD NI + K L
Sbjct: 172 VPQMWCSILK*KNRGFDVNIP*LVKNWLFHLEYALYRRICSSIGDNNINLSKFL 11
Score = 134 bits (288), Expect(2) = 2e-042
Identities = 70/117 (59%), Positives = 79/117 (67%)
Frame = -2 / -3
Query: 658 HFL*GN*QTV*EQNQGLYS*HEYRVPSHQQEPS*DQREQSL*HHTLSILVLDCNQQLKK* 479
H *GN Q+V*EQNQ LYS*H Y PS QEP *DQ QSL*HHT L+ D NQQLKK
Sbjct: 368 H*S*GNQQSV*EQNQELYS*HGYLGPSQ*QEPL*DQTGQSL*HHTSLKLLQDYNQQLKKL 189
Query: 478 *SQMTPSVGEEQHPE*EG*KP*R*YPTAYREQTLSFQVCYLQKDLRQHWRQEYLCAQ 308
S+ S QHPE E *+ *R*YP A +E LS +VC +QKDL+QHWRQ+Y Q
Sbjct: 188 LSRTIRSSNVVQHPEVEE*RL*R*YPIACQELALSSRVCLVQKDLQQHWRQQYQSVQ 18
Score = 52.8 bits (109), Expect(2) = 2e-042
Identities = 25/43 (58%), Positives = 29/43 (67%)
Frame = -2 / -3
Query: 823 ARFTA*TAPLQQQQWFQP*CMMRRQTPEKPQPQPCLPQYPIS* 695
ARF * L+QQQ FQ *C MRR P + + +PCL QYPIS*
Sbjct: 533 ARFIV*IGFLRQQQLFQL*CRMRRPMPGRRRLKPCLQQYPIS* 405
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,251,980
Number of Sequences: 8143
Number of extensions: 129882
Number of successful extensions: 1087
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 349376006
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)