TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3070703.2.1
(1214 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819880.1|CN819880 HRO4407_C04_F07ZS5 Lib AA069E1X Aven... 375 e-119
gb|CN814897.1|CN814897 HRO4503_F11_L20ZS5 Lib AA071E1X Aven... 143 5e-035
>gb|CN819880.1|CN819880 HRO4407_C04_F07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4407_C04_F07, mRNA
sequence
Length = 741
Score = 375 bits (814), Expect(2) = e-119
Identities = 155/178 (87%), Positives = 161/178 (90%)
Frame = -1 / +1
Query: 848 KQSNMAVMILRYFNVIGSDPEGRLGEAPRPELREHGRISGACFDAALGIIPGLKVRGTDY 669
K SNMAVMILRYFNVIGSDPEG LGEAPRPELREHGRISGACFDAA G IPGLKVRGTDY
Sbjct: 115 KNSNMAVMILRYFNVIGSDPEGLLGEAPRPELREHGRISGACFDAASGTIPGLKVRGTDY 294
Query: 668 STADGTCVRDYIDVTDLVDAHVKALGKAQPGKVGIYNVGTGQGRSVTEFVEACKKATGAS 489
TADGTC+RDYIDVTDLVDAHVKALGKA+P KVGIYNVGTGQGRSV EFVEACK ATGA+
Sbjct: 295 PTADGTCIRDYIDVTDLVDAHVKALGKAEPSKVGIYNVGTGQGRSVKEFVEACKAATGAT 474
Query: 488 IKVEYLARRPGDYAEVYSDPSKILMDLNWTAQYTDLGQSLAQAWKWQKAHPNGYASA* 315
IKVEYL RRPGDYAEVYS+PSKI +LNWTAQ TDL +SLA AWKWQK HP GY SA*
Sbjct: 475 IKVEYLTRRPGDYAEVYSNPSKIRDELNWTAQRTDLRESLATAWKWQKTHPGGYGSA* 648
Score = 315 bits (683), Expect(2) = 1e-095
Identities = 144/179 (80%), Positives = 150/179 (83%)
Frame = +1 / -2
Query: 313 NQADAYPFGCAFCHFHAWARLWPRSVYWAVQLRSMRIFDGSLYTSA*SPGLRARYSTLIE 492
NQAD YP GC FCHFHA ARL RSV WAVQ S RI DG L TSA*SPGLR RYSTL+
Sbjct: 650 NQADPYPPGCVFCHFHAVARLSRRSVRWAVQFSSSRILDGLL*TSA*SPGLRVRYSTLMV 471
Query: 493 APVAFLQASTNSVTDLPWPVPTL*IPTLPG*ALPRAFT*ASTRSVTSM*SLTQVPSAVE* 672
APVA L ASTNS TDLP PVPTL*IPTL G ALPRA T*ASTRSVTSM*SL QVPSAV *
Sbjct: 470 APVAALHASTNSFTDLPCPVPTL*IPTLLGSALPRALT*ASTRSVTSM*SLIQVPSAVG* 291
Query: 673 SVPRTFSPGMIPKAASKQAPDIRPCSRSSGLGASPSRPSGSDPITLKYLKIMTAIFDCF 849
SVPRTFSPGM+P AASKQAP+I PCSR+SGLGASP PSGSDPITLKYLKIMTA+F+ F
Sbjct: 290 SVPRTFSPGMVPDAASKQAPEILPCSRNSGLGASPRSPSGSDPITLKYLKIMTAMFEFF 114
Score = 224 bits (483), Expect(2) = 9e-063
Identities = 108/177 (61%), Positives = 127/177 (71%)
Frame = +3 / -1
Query: 318 G*RISVRVRLLPFPRLGKALAKIRVLGCPVEVHEDFRRIAVYLGIISRSTGKIFDLDRSS 497
G +S V LLPFP +A AKIR LGCPV++ D R+AV I+S S GK+FDLD SS
Sbjct: 645 GRSVSPGVCLLPFPCRREAFAKIRALGCPVQLVADLGRVAVNFSIVSWSPGKVFDLDGSS 466
Query: 498 RCLLASFYKLSH*STLACSDIVDSNFAWLSFTKSFYVSIYKICDIDVVSNASSICSGVIG 677
LA FYKL +* TL ++IVDSNFA LSFTKSF +SI +ICDI VV N SSI S V+
Sbjct: 465 CRRLARFYKLLY*PTLPRANIVDSNFARLSFTKSFDMSIDEICDIYVVPNTSSISSRVVR 286
Query: 678 STNL*PWNDSQSCIKASTGYPSMLA*LWPWSFS*PSLWV*SNHVEVS*DHDSHI*LL 848
S+NL PWN S CI+ ST PS+LA LWPWSF SL V*SNH+EV *DHDSH+*+L
Sbjct: 285 SSNLQPWNGS*RCIETSTRNPSVLAQLWPWSFPEESLRV*SNHIEVP*DHDSHV*VL 115
Score = 172 bits (370), Expect(2) = 5e-053
Identities = 97/176 (55%), Positives = 109/176 (61%)
Frame = -2 / +2
Query: 841 QIWLS*S*DTST*LDQTQRDG*EKLQGQSYASMDGYPVLALMQLWESFQG*RFVEPITPL 662
Q WLS*S* TS *LDQT+RD KLQGQS AS +G+ VL MQ E FQG*RF E T L
Sbjct: 122 QTWLS*S*GTSM*LDQTRRDSSGKLQGQSCASTEGFLVLVSMQRQEPFQG*RFEERTTLL 301
Query: 661 QMELALETTSMSQIXXXXXXXXXXXLSQAKLESTMSEQAKVDQ*LSL*KLARRQRELLSR 482
MEL L TT MSQI LS AKLESTM + KV Q* SL*K ARR++EL SR
Sbjct: 302 LMELVLGTT*MSQISSMLMSKLLVKLSLAKLESTMLARGKVGQ*RSL*KRARRRQELPSR 481
Query: 481 SNILPVDLEIMPRYTAIRRKSSWTSTGQPNTRILAKALPXXXXXXXXXXTDMRQPD 314
SN LP D E M ++TA R +S+ + TGQPN RI AKA D +PD
Sbjct: 482 SNTLPGDQETMLKFTATRPRSATS*TGQPNARIFAKASRRHGNGRRHTPGDTDRPD 649
Score = 94.5 bits (200), Expect(3) = 3e-041
Identities = 43/84 (51%), Positives = 56/84 (66%)
Frame = -3 / +3
Query: 564 LQCRNRPR*ISD*VCRSLQEGNGSFYQGRISCP*TWRLCRGIQRSVENPHGPQLDSPIHG 385
LQC + R*+S VCRS+Q G+ S++QGRI P T RLC +Q+ V++P +LDSP HG
Sbjct: 399 LQCWHGAR*VSKGVCRSVQGGDRSYHQGRIPYPETRRLC*SLQQPVQDPRRAELDSPTHG 578
Query: 384 SWPKPCPGVEMAEGAPERICVSLI 313
S KP G+EMAE P I + LI
Sbjct: 579 SSRKPRDGMEMAEDTPRGIRIGLI 650
Score = 91.8 bits (194), Expect(3) = 4e-041
Identities = 43/73 (58%), Positives = 50/73 (68%)
Frame = +2 / -3
Query: 521 QTQSLIYLGLFRHCRFQLCLAELYQELLREHLQDL*HRCSL*RKFHLQWSNRFHEPLALE 700
QT L YL +HCRFQLC A+LYQEL EH +DL*H CS * KFH Q + F EP ALE
Sbjct: 442 QTPLLTYLAPCQHCRFQLC*AQLYQEL*HEHRRDL*HLCSP*YKFHQQ*GSPFLEPSALE 263
Query: 701 *FPKLHQSKHRIS 739
F LH++KH+ S
Sbjct: 262 WFLTLHRNKHQKS 224
Score = 70.3 bits (147), Expect(2) = e-119
Identities = 28/38 (73%), Positives = 32/38 (84%)
Frame = -1 / +1
Query: 947 MPIVETTPQNPINPYGKAKKMAEDIILDFTKSKKQSNM 834
MPI ETTPQ PINPYGKAKKM+EDIILDF+K+ + M
Sbjct: 25 MPITETTPQVPINPYGKAKKMSEDIILDFSKNSNMAVM 138
Score = 67.5 bits (141), Expect(3) = 3e-041
Identities = 28/54 (51%), Positives = 32/54 (59%)
Frame = -3 / +3
Query: 843 IKYGCHDLKILQRDWIRPRGXXXXXXXXXXXXAWTDIRCLL*CSFGNHSRAKGS 682
+K+GCHDLK+LQ DWIRP G A D CL CS NHSRA+GS
Sbjct: 120 LKHGCHDLKVLQCDWIRPGGTPRGSSKARVARARKDFWCLFRCSVRNHSRAEGS 281
Score = 57.9 bits (120), Expect(3) = 4e-041
Identities = 28/44 (63%), Positives = 31/44 (70%)
Frame = +1 / -2
Query: 817 LKIMTAIFDCFFDFVKSKMMSSAIFFALPYGLIGF*GVVSTIGI 948
LK + + F F KS+MMSS IFFA PYGLIG *GVVS IGI
Sbjct: 155 LKYLKIMTAMFEFFEKSRMMSSDIFFAFPYGLIGT*GVVSVIGI 24
Score = 52.4 bits (108), Expect(3) = 4e-041
Identities = 27/56 (48%), Positives = 32/56 (57%)
Frame = +2 / -3
Query: 314 IRLTHIRSGAPSAISTPGQGFGQDPCIGLSS*GP*GFSTDRCIPRHNLQVYGQDIR 481
IR I G SAIS P +GF +DPC+GLSS G T C +H+L V G IR
Sbjct: 649 IRPIRIPRGVSSAISMPSRGFREDPCVGLSSSARRGSWTGCCKLQHSLLVSG*GIR 482
Score = 51.5 bits (106), Expect(2) = 5e-053
Identities = 20/31 (64%), Positives = 24/31 (77%)
Frame = -3 / +3
Query: 948 DAYCRNNSSESYQSIW*GKEDGGGHHLRFYK 856
+AY RNN+S SYQSIW K+D GHH RF+K
Sbjct: 24 NAYHRNNTSGSYQSIWESKKDVRGHHSRFFK 116
Score = 50.1 bits (103), Expect(2) = 1e-095
Identities = 20/29 (68%), Positives = 22/29 (75%)
Frame = +3 / -1
Query: 861 KI*DDVLRHLLCLTIWIDRILRSCFYNRH 947
KI*+DVL HL C IWIDR LR CF +RH
Sbjct: 111 KI*NDVL*HLFCFPIWIDRNLRCCFCDRH 25
Score = 40.5 bits (82), Expect(3) = 3e-041
Identities = 19/35 (54%), Positives = 25/35 (71%)
Frame = -2 / +2
Query: 949 RCLL*KQLLRILSIHMVRQRRWRRTSS*ILQNQRS 845
+CL KQ LR LSIHM +Q+R +RTS *I Q ++
Sbjct: 23 QCLSQKQHLRFLSIHMGKQKRCQRTSF*IFQRTQT 127
Score = 32.2 bits (64), Expect(2) = 9e-063
Identities = 18/28 (64%), Positives = 19/28 (67%)
Frame = +2 / -3
Query: 863 NLR*CPPPSSLPYHMD**DSEELFLQ*A 946
NL *CP S L HMD**+ E LFL *A
Sbjct: 109 NLE*CPLTSFLLSHMD**EPEVLFL**A 26
>gb|CN814897.1|CN814897 HRO4503_F11_L20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_F11_L20, mRNA sequence
Length = 610
Score = 143 bits (307), Expect = 5e-035
Identities = 54/74 (72%), Positives = 65/74 (87%)
Frame = -1 / +1
Query: 542 GRSVTEFVEACKKATGASIKVEYLARRPGDYAEVYSDPSKILMDLNWTAQYTDLGQSLAQ 363
GRSV EFV+ACKKATG IKVEYL+RRPGDYAEV+SDP+KI +LNWTAQ+TDL +SL+
Sbjct: 70 GRSVKEFVDACKKATGVDIKVEYLSRRPGDYAEVFSDPTKINSELNWTAQHTDLKESLSV 249
Query: 362 AWKWQKAHPNGYAS 321
AW+WQK+HP GY +
Sbjct: 250 AWRWQKSHPRGYGT 291
Score = 104 bits (221), Expect = 4e-023
Identities = 49/71 (69%), Positives = 53/71 (74%)
Frame = +1 / -3
Query: 331 PFGCAFCHFHAWARLWPRSVYWAVQLRSMRIFDGSLYTSA*SPGLRARYSTLIEAPVAFL 510
P GC FCH HA RL RSV AVQL S+ IF GSL TSA*SPGL RYSTL+ PVAFL
Sbjct: 281 PRGCDFCHLHATDRLSLRSVC*AVQLSSLLIFVGSLNTSA*SPGLLLRYSTLMSTPVAFL 102
Query: 511 QASTNSVTDLP 543
QASTNS+T+ P
Sbjct: 101 QASTNSLTERP 69
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 13,557,649
Number of Sequences: 8143
Number of extensions: 192895
Number of successful extensions: 1008
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 435310200
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)