TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3067516.2.1
(605 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN820067.1|CN820067 HRO4410_G02_M04ZS5 Lib AA069E1X Aven... 225 3e-063
>gb|CN820067.1|CN820067 HRO4410_G02_M04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4410_G02_M04, mRNA
sequence
Length = 689
Score = 225 bits (486), Expect(3) = 3e-063
Identities = 95/139 (68%), Positives = 110/139 (79%)
Frame = -2 / +1
Query: 604 GNASIEERLKSIHPDVVKQMREEVINLIPKVIYADPRSKLETLKDAFDVSIEAIINKVTK 425
GN SIE L+SIHPDVVKQMREEVINLIP+VIYADPRSKLETLKDAFDVS+ AIINKVT+
Sbjct: 46 GNVSIEGILRSIHPDVVKQMREEVINLIPRVIYADPRSKLETLKDAFDVSVGAIINKVTQ 225
Query: 424 LRRDIIAGQEDKDFIEENSWKYSLLDDGQQAIGPHKWDPFFXXXXXXXXXXXXXSAEAAK 245
+RRDI++ EDKDFIEENSWKY+LL++GQ+ IGPH+WDPFF S AAK
Sbjct: 226 MRRDIVSNSEDKDFIEENSWKYALLEEGQRTIGPHEWDPFFSKPKDKGADSSNSSVGAAK 405
Query: 244 NSWKSEQRGQD*TMSWSSL 188
NSWK EQ Q+* ++L
Sbjct: 406 NSWKIEQGDQN*IAHLAAL 462
Score = 211 bits (456), Expect(3) = 4e-058
Identities = 98/127 (77%), Positives = 103/127 (81%)
Frame = +3 / -1
Query: 225 CSLFQEFLAASADEPLESPPLSLGFEKNGSHL*GPIACCPSSNRLYFQLFSSIKSLSSWP 404
CS+FQEFLAA DE LES PLSLG EKNGSH GPI CPSSNR YFQLFSS+KSLSS
Sbjct: 425 CSIFQEFLAAPTDELLESAPLSLGLEKNGSHSCGPIVRCPSSNRAYFQLFSSMKSLSSLF 246
Query: 405 AMISLLNFVTLLMIASMETSNASFNVSSFDLGSAYITFGIRLMTSSLICLTTSG*ILFSL 584
ISLL VTLLMIA ETSNASF VSSFDLGSAYIT G+RL+TSSLICLTTSG IL S+
Sbjct: 245 ETISLLICVTLLMIAPTETSNASFRVSSFDLGSAYITLGMRLITSSLICLTTSGWILLSI 66
Query: 585 SSMLAFP 605
SML FP
Sbjct: 65 PSMLTFP 45
Score = 167 bits (360), Expect = 1e-042
Identities = 77/131 (58%), Positives = 88/131 (67%)
Frame = +2 / -3
Query: 212 ILSSLFTFPRILGSFCR*TTRISTFIFRLREEWIPFVRSNCLLSIV*QTILPAILFDKIF 391
IL SLF FPRILG R* TRI F+ LREEWIPF+RS+C LS *Q+ILPAILF++IF
Sbjct: 438 ILVSLFNFPRILGCSNR*ITRICPFVLGLREEWIPFMRSDCSLSFF*QSILPAILFNEIF 259
Query: 392 VFLACDDISSQLCHFVNDRLYGNIKCIFQCLQFRSWIGIYHLWYQIXXXXXXXXXXIWVN 571
+F DDISS LCH VND + NIK I Q Q R WI IYH WY+I IW++
Sbjct: 258 IFAV*DDISSHLCHLVNDCPHRNIKRILQGFQLRPWISIYHPWYEIDNLFPHLLDDIWMD 79
Query: 572 TL*PLLNAGIS 604
T * LNA IS
Sbjct: 78 TS*YPLNADIS 46
Score = 150 bits (323), Expect = 1e-037
Identities = 78/131 (59%), Positives = 93/131 (70%)
Frame = -3 / +2
Query: 603 EMPALRRG*RVFTQMWSSK*ERRSSI*YQR*YMPIQDRNWRH*KMHLMFP*RRSLTK*QS 424
EM ALR * V QM SS+* +R SI YQ *YM IQ R+W+ *+M LMF +SLT+*
Sbjct: 47 EMSALRGY*EVSIQMSSSR*GKRLSISYQG*YMLIQGRSWKP*RMRLMFRWGQSLTR*HR 226
Query: 423 *EEISSQAKKTKILSKRIAGSIVC*TMDSRQLDLTNGIHSSRSLKIKVEILVVHLQKLPR 244
*EEISSQ K KI KRIAGS++C* DS Q D NGIHSS S + K +ILV+HL + PR
Sbjct: 227 *EEISSQTAKIKISLKRIAGSMLC*KKDSEQSDRMNGIHSSLSPRTKGQILVIHLLEQPR 406
Query: 243 ILGKVNREDRI 211
ILGK+N+E RI
Sbjct: 407 ILGKLNKETRI 439
Score = 26.3 bits (51), Expect(3) = 3e-063
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -2 / +1
Query: 112 DPVVMVIMNIIDDLCHPAVV*SHSTKMLWKKFGR 11
D VV+VIM ++DD V + M KK+ R
Sbjct: 541 DAVVLVIMYVLDDPAPTGVRSGATKSMFEKKYDR 642
Score = 24.4 bits (47), Expect(3) = 4e-058
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = +2 / -3
Query: 74 IINDVHDHHNY 106
II ++HDH NY
Sbjct: 579 IIKNIHDHQNY 547
Score = 23.5 bits (45), Expect(3) = 3e-063
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -3 / +3
Query: 177 KGVELAILSEDALMHIGC 124
+G + AIL+E ALM I C
Sbjct: 474 EGSKRAILAEGALMEIRC 527
Score = 22.1 bits (42), Expect(3) = 4e-058
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +3 / -3
Query: 126 NLYASEHLQRV*L 164
N++ SEHLQR *L
Sbjct: 525 NVFPSEHLQRG*L 487
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 6,643,836
Number of Sequences: 8143
Number of extensions: 83809
Number of successful extensions: 994
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 194851479
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)