TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3067450.2.1
(724 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN814875.1|CN814875 HRO4503_D10_H19ZS5 Lib AA071E1X Aven... 154 2e-038
gb|CN815034.1|CN815034 HRO4505_B06_C11ZS5 Lib AA071E1X Aven... 152 7e-038
gb|CN816101.1|CN816101 HRO4517_A05_A09ZS5 Lib AA071E1X Aven... 109 1e-036
gb|CN816873.1|CN816873 HRO4525_G05_M09ZS5 Lib AA071E1X Aven... 136 5e-033
gb|CN821053.1|CN821053 HRO4427_A04_B07ZS5 Lib AA069E1X Aven... 133 3e-032
gb|CN815850.1|CN815850 HRO4511_B09_D17ZS5 Lib AA071E1X Aven... 133 4e-032
gb|CN814789.1|CN814789 HRO4502_E05_I10ZS5 Lib AA071E1X Aven... 117 2e-027
gb|CN816425.1|CN816425 HRO4520_F02_L04ZS5 Lib AA071E1X Aven... 100 4e-022
gb|CN820644.1|CN820644 HRO4415_G07_N13ZS5 Lib AA069E1X Aven... 64 1e-021
>gb|CN814875.1|CN814875 HRO4503_D10_H19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4503_D10_H19, mRNA sequence
Length = 846
Score = 154 bits (330), Expect = 2e-038
Identities = 63/96 (65%), Positives = 76/96 (79%)
Frame = -2 / +1
Query: 462 GDSECVTILKNCKKAIPPRDAGGKVIIVDTVVGAGPPNLKNKETQVMSDLFFMIVNGLER 283
GD +CV ILK CK+AIP RDAGGKVIIVD VVG+GP ++ +ETQV DLF M + G+ER
Sbjct: 10 GDEDCVKILKKCKEAIPSRDAGGKVIIVDMVVGSGPNDIVTRETQVFFDLFIMYLEGIER 189
Query: 282 DEQEWRNIIFEAGFSDYIIIPVLGVRSIIELYP*NI 175
+E EW+ I EAGFS+Y II VLGVRS+IELYP* +
Sbjct: 190 EEFEWKKIFMEAGFSEYKIISVLGVRSVIELYP*TL 297
Score = 109 bits (233), Expect = 5e-025
Identities = 54/94 (57%), Positives = 60/94 (63%)
Frame = +2 / -2
Query: 182 QGYSSMIDRTPKTGMIM*SLKPASKMMFLHSCSSLSRPLTIIKKRSDMTCVSLFLRFGGP 361
QG SS+ D TP T MI+ SL PAS +F HS SS S P + KRS TCVS GP
Sbjct: 290 QG*SSITDLTPSTDMILYSLNPASMNIFFHSNSSRSIPSRYMIKRSKKTCVSRVTMSFGP 111
Query: 362 APTTVSTIITLPPASRGGIAFLQFFRIVTHSESP 463
PTT+STIIT PPAS GIA L FF I+T S SP
Sbjct: 110 DPTTISTIITFPPASLEGIASLHFFSILTQSSSP 9
>gb|CN815034.1|CN815034 HRO4505_B06_C11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4505_B06_C11, mRNA sequence
Length = 629
Score = 152 bits (326), Expect = 7e-038
Identities = 59/143 (41%), Positives = 89/143 (62%)
Frame = -2 / +1
Query: 603 IQCTVLDLAHVVATAPACAGLSFVAGDMFEAIPPANAVFLKWIMHDWGDSECVTILKNCK 424
I+CTVLDL V+ P+ +++VAGD+F ++P + AV LK ++H W D +CV IL C+
Sbjct: 64 IKCTVLDLPKVIEKVPSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDDDCVKILSQCR 243
Query: 423 KAIPPRDAGGKVIIVDTVVGAGPPNLKNKETQVMSDLFFMIVNGLERDEQEWRNIIFEAG 244
AIP R+ GGKVII+D +VG + + +M + G +RDE +WR + +AG
Sbjct: 244 SAIPSREEGGKVIIIDILVGPSLGPIMFEAQILMDMAMLVNTRGRQRDENDWRQLFMKAG 423
Query: 243 FSDYIIIPVLGVRSIIELYP*NI 175
FSDY ++ LG R + E+Y * +
Sbjct: 424 FSDYKVVKKLGARGVFEVYK*RV 492
>gb|CN816101.1|CN816101 HRO4517_A05_A09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4517_A05_A09, mRNA sequence
Length = 652
Score = 109 bits (232), Expect(2) = 1e-036
Identities = 41/79 (51%), Positives = 57/79 (72%)
Frame = -2 / +3
Query: 603 IQCTVLDLAHVVATAPACAGLSFVAGDMFEAIPPANAVFLKWIMHDWGDSECVTILKNCK 424
++CTVLDL V+ AP +++VAGD+F +PP+ AV LK ++H W D +CV IL C+
Sbjct: 30 LKCTVLDLPKVIEKAPPHDVINYVAGDLFHTVPPSQAVMLKLVLHFWSDEDCVKILAQCR 209
Query: 423 KAIPPRDAGGKVIIVDTVV 367
KAIP R+ GGKVII++ VV
Sbjct: 210 KAIPSREEGGKVIIIEIVV 266
Score = 58.8 bits (122), Expect(2) = 1e-036
Identities = 22/46 (47%), Positives = 32/46 (69%)
Frame = -2 / +3
Query: 315 LFFMIVNGLERDEQEWRNIIFEAGFSDYIIIPVLGVRSIIELYP*N 178
L + G +RDE+ WR + +AGF+DY I+ LG RS+IE+YP*+
Sbjct: 318 LMMVNTRGGQRDEKHWRELFMQAGFTDYKIVKKLGARSVIEVYP*D 455
>gb|CN816873.1|CN816873 HRO4525_G05_M09ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4525_G05_M09, mRNA sequence
Length = 662
Score = 136 bits (291), Expect = 5e-033
Identities = 52/133 (39%), Positives = 81/133 (60%)
Frame = -2 / +2
Query: 573 VVATAPACAGLSFVAGDMFEAIPPANAVFLKWIMHDWGDSECVTILKNCKKAIPPRDAGG 394
V+ P+ +++VAGD+F ++P + AV LK ++H W D +CV IL C+ AIP R+ GG
Sbjct: 41 VIEKVPSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDDDCVKILSQCRSAIPSREEGG 220
Query: 393 KVIIVDTVVGAGPPNLKNKETQVMSDLFFMIVNGLERDEQEWRNIIFEAGFSDYIIIPVL 214
KVII+D +VG + + +M + G +RDE +WR + +AGFSDY ++ L
Sbjct: 221 KVIIIDILVGPSLGPIMFEAQILMDMAMLVNTRGRQRDENDWRQLFMKAGFSDYKVVKKL 400
Query: 213 GVRSIIELYP*NI 175
G R + E+Y * +
Sbjct: 401 GARGVFEVYK*RV 439
>gb|CN821053.1|CN821053 HRO4427_A04_B07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4427_A04_B07, mRNA
sequence
Length = 644
Score = 133 bits (285), Expect = 3e-032
Identities = 52/131 (39%), Positives = 79/131 (60%)
Frame = -2 / +3
Query: 579 AHVVATAPACAGLSFVAGDMFEAIPPANAVFLKWIMHDWGDSECVTILKNCKKAIPPRDA 400
A + P+ +++VAGD+F ++P + AV LK ++H W D +CV IL C+ AIP R+
Sbjct: 3 ARGIDKVPSDGIVNYVAGDLFHSVPKSQAVLLKLVLHHWSDEDCVKILSQCRNAIPSREK 182
Query: 399 GGKVIIVDTVVGAGPPNLKNKETQVMSDLFFMIVNGLERDEQEWRNIIFEAGFSDYIIIP 220
GGKVII+D +VG + + +M + G +RDE +WR + +AGFSDY I+
Sbjct: 183 GGKVIIIDILVGPSLGPIMFEAQILMDMAMLVNTRGRQRDENDWRQLFMKAGFSDYKIVK 362
Query: 219 VLGVRSIIELY 187
LG R + E+Y
Sbjct: 363 KLGARGVFEVY 395
>gb|CN815850.1|CN815850 HRO4511_B09_D17ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_B09_D17, mRNA sequence
Length = 621
Score = 133 bits (284), Expect = 4e-032
Identities = 51/121 (42%), Positives = 75/121 (61%)
Frame = -2 / +2
Query: 537 FVAGDMFEAIPPANAVFLKWIMHDWGDSECVTILKNCKKAIPPRDAGGKVIIVDTVVGAG 358
+VAGD+F ++P + AV LK ++H W D +CV IL CK AIP R+ GGKVII+D +VG
Sbjct: 23 YVAGDLFHSVPKSQAVMLKLVLHRWSDEDCVKILSQCKSAIPSREEGGKVIIIDILVGPS 202
Query: 357 PPNLKNKETQVMSDLFFMIVNGLERDEQEWRNIIFEAGFSDYIIIPVLGVRSIIELYP*N 178
+ + +M + G +RDE +WR + +AGFSDY I+ +G R + E+Y *
Sbjct: 203 LGPIMFEAQVLMDMAMLVNTRGRQRDENDWRQLFMKAGFSDYKIVKKVGARGVFEVYK*R 382
Query: 177 I 175
+
Sbjct: 383 V 385
>gb|CN814789.1|CN814789 HRO4502_E05_I10ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_E05_I10, mRNA sequence
Length = 596
Score = 117 bits (250), Expect = 2e-027
Identities = 45/109 (41%), Positives = 66/109 (60%)
Frame = -2 / -3
Query: 513 AIPPANAVFLKWIMHDWGDSECVTILKNCKKAIPPRDAGGKVIIVDTVVGAGPPNLKNKE 334
++P + AV LK ++H W D +CV IL C+ AIP R+ GGKVII+D +VG + +
Sbjct: 591 SVPKSQAVLLKLVLHHWSDEDCVKILSQCRNAIPSREKGGKVIIIDILVGPSLGPIMFEA 412
Query: 333 TQVMSDLFFMIVNGLERDEQEWRNIIFEAGFSDYIIIPVLGVRSIIELY 187
+M + G +RDE +WR + +AGFSDY I+ LG R + E+Y
Sbjct: 411 QILMDMAMLVNTRGRQRDENDWRQLFMKAGFSDYKIVKKLGARGVFEVY 265
>gb|CN816425.1|CN816425 HRO4520_F02_L04ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_F02_L04, mRNA sequence
Length = 538
Score = 100 bits (212), Expect = 4e-022
Identities = 39/93 (41%), Positives = 55/93 (59%)
Frame = -2 / +1
Query: 465 WGDSECVTILKNCKKAIPPRDAGGKVIIVDTVVGAGPPNLKNKETQVMSDLFFMIVNGLE 286
W D +CV IL C+ AIP R+ GGKVII+D +VG + + +M + G +
Sbjct: 13 WSDEDCVKILSQCRNAIPSREKGGKVIIIDILVGPSLGPIMFEAQILMDMAMLVNTRGRQ 192
Query: 285 RDEQEWRNIIFEAGFSDYIIIPVLGVRSIIELY 187
RDE +WR + +AGFSDY I+ LG R + E+Y
Sbjct: 193 RDENDWRQLFMKAGFSDYKIVKKLGARGVFEVY 291
>gb|CN820644.1|CN820644 HRO4415_G07_N13ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4415_G07_N13, mRNA
sequence
Length = 614
Score = 64.3 bits (134), Expect(2) = 1e-021
Identities = 22/40 (55%), Positives = 30/40 (75%)
Frame = -2 / +2
Query: 486 LKWIMHDWGDSECVTILKNCKKAIPPRDAGGKVIIVDTVV 367
+K ++H W D +CV IL CKKAIP R+ GGKVI++D +V
Sbjct: 11 VKLVLHHWSDEDCVKILAQCKKAIPSREEGGKVIVIDILV 130
Score = 53.3 bits (110), Expect(2) = 1e-021
Identities = 19/43 (44%), Positives = 29/43 (67%)
Frame = -2 / +2
Query: 306 MIVNGLERDEQEWRNIIFEAGFSDYIIIPVLGVRSIIELYP*N 178
++ NG +RDE +W I +AGF+ Y I+ LG R + E+YP*+
Sbjct: 188 VLTNGRQRDESDWAEIFTKAGFTGYNIVKKLGARGVFEVYP*D 316
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,001,980
Number of Sequences: 8143
Number of extensions: 139977
Number of successful extensions: 1438
Number of sequences better than 1.0e-020: 9
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 239815586
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)