TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3064721.2.2
(595 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Aven... 203 2e-053
gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Aven... 191 8e-050
gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Aven... 162 5e-041
gb|CN815983.1|CN815983 HRO4515_F02_L03ZS5 Lib AA071E1X Aven... 91 6e-021
gb|CN814825.1|CN814825 HRO4502_H06_O12ZS5 Lib AA071E1X Aven... 90 8e-021
>gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_A06_B12, mRNA sequence
Length = 570
Score = 203 bits (438), Expect = 2e-053
Identities = 75/123 (60%), Positives = 94/123 (76%)
Frame = +3 / +1
Query: 3 NMFDLGDWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWNRTFPLDRSWHQLGLGY 182
NMFDL +W+K++IT +YH WQ LNENRLLWKLGTLP GL+TF+ T PLD+SWH LGLGY
Sbjct: 112 NMFDLEEWKKKDITGIYHRWQTLNENRLLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGY 291
Query: 183 NPNVNVKDIRRAAVIHYNGNLKPWLEIGLPKYRKYWSAHVNYDQVFLRECNINP*TANSG 362
NP V +I AAVIHYNGN+KPWLEI + KYR YW+ ++NY+ ++ C I+ *T
Sbjct: 292 NPTVEHSEIDSAAVIHYNGNMKPWLEIAMTKYRPYWTKYINYEHSYVHGCKISQ*TVARE 471
Query: 363 AWF 371
A+F
Sbjct: 472 AFF 480
Score = 125 bits (267), Expect = 8e-030
Identities = 64/122 (52%), Positives = 73/122 (59%)
Frame = -1 / -2
Query: 367 HAPLFAVQGFMLHSRRNT*S*LTCADQYLRYFGNPISSQGFRFPL*WMTAARLISLTLTF 188
+A L V +LH S*L Q RYF ISS GF FPL*WMTAA IS T
Sbjct: 476 NASLATVY*LILHPWT*ECS*LMYFVQ*GRYFVIAISSHGFMFPL*WMTAALSISECSTV 297
Query: 187 GLYPSPN*CHERSRGNVRFQKVTRPAGRVPSFHNSLFSFNFCQVW*TSVMFCFLQSPKSN 8
GLYP+P+ CH+ S G V KV RP G VPSFH+SLFSF C +W* VM F S +SN
Sbjct: 296 GLYPNPSTCHDLSSGCVSL*KVIRPGGSVPSFHSSLFSFRVCHLW*IPVMSFFFHSSRSN 117
Query: 7 MF 2
+F
Sbjct: 116 IF 111
>gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4423_D02_H03, mRNA
sequence
Length = 733
Score = 191 bits (412), Expect = 8e-050
Identities = 66/119 (55%), Positives = 91/119 (76%)
Frame = +3 / +1
Query: 3 NMFDLGDWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFWNRTFPLDRSWHQLGLGY 182
N+FDL W+ ++IT +YH WQ +NE+R+LWKLGTLP GL+TF+ T PLD+SWH LGLGY
Sbjct: 223 NIFDLKQWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGY 402
Query: 183 NPNVNVKDIRRAAVIHYNGNLKPWLEIGLPKYRKYWSAHVNYDQVFLRECNINP*TANS 359
NP+++ +I AAV HYNGN+KPWLE+ + KYR YW+ ++ YD ++R CN++ * S
Sbjct: 403 NPSIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGCNLSD*KQES 579
Score = 95.9 bits (203), Expect = 5e-021
Identities = 52/111 (46%), Positives = 59/111 (53%)
Frame = -1 / -3
Query: 334 LHSRRNT*S*LTCADQYLRYFGNPISSQGFRFPL*WMTAARLISLTLTFGLYPSPN*CHE 155
LH R *S QY RY S+ GF FPL* TAA IS GLYP+P CH+
Sbjct: 554 LHPRI*G*SYFIYLVQYGRYLVIASSNHGFIFPL**ATAALSISERSMLGLYPNPRTCHD 375
Query: 154 RSRGNVRFQKVTRPAGRVPSFHNSLFSFNFCQVW*TSVMFCFLQSPKSNMF 2
SRG V V RP G VPSF +L SF FC +W* V+ F +S MF
Sbjct: 374 LSRGCVSL*NVMRPGGSVPSFQRTLSSFMFCHLW*IPVISLFFHCFRSKMF 222
>gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_D01_H01, mRNA sequence
Length = 653
Score = 162 bits (348), Expect = 5e-041
Identities = 56/94 (59%), Positives = 78/94 (82%)
Frame = +3 / +1
Query: 63 QKLNENRLLWKLGTLPAGLVTFWNRTFPLDRSWHQLGLGYNPNVNVKDIRRAAVIHYNGN 242
Q NENRLLWKLGTLP GL+TF++ T PLD+SWH LGLGYNP++++++IR AAV+H+NGN
Sbjct: 331 QTQNENRLLWKLGTLPPGLITFYSTTKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGN 510
Query: 243 LKPWLEIGLPKYRKYWSAHVNYDQVFLRECNINP 344
+KPWL+IG+ ++R W+ +V+Y F+R+CN P
Sbjct: 511 MKPWLDIGMNQFRHLWTKYVDYGDSFIRQCNFAP 612
>gb|CN815983.1|CN815983 HRO4515_F02_L03ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_F02_L03, mRNA sequence
Length = 668
Score = 90.9 bits (192), Expect(2) = 6e-021
Identities = 33/77 (42%), Positives = 52/77 (67%)
Frame = +3 / +1
Query: 117 LVTFWNRTFPLDRSWHQLGLGYNPNVNVKDIRRAAVIHYNGNLKPWLEIGLPKYRKYWSA 296
L++F + +PLD GLGY+ ++ + R +A +HYNGN+KPWLE+G+ +Y+KYW
Sbjct: 385 LLSFQHLIYPLDERLILSGLGYDYGIDPEVARSSASLHYNGNMKPWLELGILEYKKYWRR 564
Query: 297 HVNYDQVFLRECNINP* 347
+ + F+ ECN+NP*
Sbjct: 565 FLTREDRFMDECNVNP* 615
Score = 24.0 bits (46), Expect(2) = 6e-021
Identities = 7/17 (41%), Positives = 11/17 (64%)
Frame = +3 / +1
Query: 3 NMFDLGDWRKQNITEVY 53
N+ +L W+K +TE Y
Sbjct: 265 NVINLDKWKKHKVTEKY 315
>gb|CN814825.1|CN814825 HRO4502_H06_O12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4502_H06_O12, mRNA sequence
Length = 837
Score = 89.5 bits (189), Expect(2) = 8e-021
Identities = 33/77 (42%), Positives = 51/77 (66%)
Frame = +3 / +1
Query: 117 LVTFWNRTFPLDRSWHQLGLGYNPNVNVKDIRRAAVIHYNGNLKPWLEIGLPKYRKYWSA 296
L++F + +PLD GLGY+ ++ + R +A +HYNGN+KPWLE+G+ +Y+KYW
Sbjct: 322 LLSFQHLIYPLDERLTLSGLGYDYGIDPEVARSSASLHYNGNMKPWLELGILEYKKYWRR 501
Query: 297 HVNYDQVFLRECNINP* 347
+ + F+ ECN NP*
Sbjct: 502 FLTREDRFMDECNANP* 552
Score = 24.9 bits (48), Expect(2) = 8e-021
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +3 / +1
Query: 3 NMFDLGDWRKQNITEVY 53
N+ +L WRK +TE Y
Sbjct: 202 NVINLDKWRKHKVTENY 252
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,256,325
Number of Sequences: 8143
Number of extensions: 104580
Number of successful extensions: 1370
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 189949555
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)