TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3064721.2.1
(689 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Aven... 111 3e-048
gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Aven... 102 3e-045
gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Aven... 70 5e-030
>gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_A06_B12, mRNA sequence
Length = 570
Score = 111 bits (236), Expect(3) = 3e-048
Identities = 39/52 (75%), Positives = 46/52 (88%)
Frame = +2 / +1
Query: 2 HACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFW 157
+ACGWAYGMNMFDL W+K++IT +YH WQ LNENRLLWKLGTLP GL+TF+
Sbjct: 85 NACGWAYGMNMFDLEEWKKKDITGIYHRWQTLNENRLLWKLGTLPPGLMTFY 240
Score = 75.8 bits (159), Expect(2) = 1e-024
Identities = 30/50 (60%), Positives = 35/50 (70%)
Frame = -3 / -2
Query: 153 KVTRPAGRVPSFHNSLFSFNFCQVW*TSVMFCFLHPDKSNMFMPYAQPQA 4
KV RP G VPSFH+SLFSF C +W* VM F H +SN+F+P AQP A
Sbjct: 236 KVIRPGGSVPSFHSSLFSFRVCHLW*IPVMSFFFHSSRSNIFIP*AQPHA 87
Score = 66.6 bits (139), Expect(3) = 3e-048
Identities = 24/37 (64%), Positives = 28/37 (75%)
Frame = +1 / +1
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP V +I A VIH+NGN+K
Sbjct: 247 THPLDKSWHVLGLGYNPTVEHSEIDSAAVIHYNGNMK 357
Score = 51.9 bits (107), Expect(2) = 1e-024
Identities = 23/46 (50%), Positives = 27/46 (58%)
Frame = -1 / -2
Query: 302 WAIQSQARSFKFPLKWMTVARLISFSLTFGLYPSPS*CHERSRGNV 165
+ I + F FPL WMT A IS T GLYP+PS CH+ S G V
Sbjct: 383 FVIAISSHGFMFPL*WMTAALSISECSTVGLYPNPSTCHDLSSGCV 246
Score = 47.8 bits (98), Expect(3) = 3e-048
Identities = 15/33 (45%), Positives = 23/33 (69%)
Frame = +2 / +1
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP*T 379
WLEI + KYR YW+ ++NY+ ++ C I+ *T
Sbjct: 361 WLEIAMTKYRPYWTKYINYEHSYVHGCKISQ*T 459
>gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4423_D02_H03, mRNA
sequence
Length = 733
Score = 102 bits (218), Expect(3) = 3e-045
Identities = 34/52 (65%), Positives = 45/52 (86%)
Frame = +2 / +1
Query: 2 HACGWAYGMNMFDLSGWRKQNITEVYHTWQKLNENRLLWKLGTLPAGLVTFW 157
+ACGWAYGMN+FDL W+ ++IT +YH WQ +NE+R+LWKLGTLP GL+TF+
Sbjct: 196 NACGWAYGMNIFDLKQWKNKDITGIYHKWQNMNEDRVLWKLGTLPPGLMTFY 351
Score = 65.2 bits (136), Expect(3) = 3e-045
Identities = 22/37 (59%), Positives = 29/37 (78%)
Frame = +1 / +1
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ +I A V H+NGN+K
Sbjct: 358 THPLDKSWHVLGLGYNPSIDRSEIDNAAVAHYNGNMK 468
Score = 47.3 bits (97), Expect(3) = 3e-045
Identities = 14/36 (38%), Positives = 24/36 (66%)
Frame = +2 / +1
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP*TANS 388
WLE+ + KYR YW+ ++ YD +++ CN++ * S
Sbjct: 472 WLELAMTKYRPYWTRYIKYDHPYIRGCNLSD*KQES 579
>gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_D01_H01, mRNA sequence
Length = 653
Score = 70.3 bits (147), Expect(3) = 5e-030
Identities = 24/37 (64%), Positives = 32/37 (86%)
Frame = +1 / +1
Query: 166 TFPLDRSWHQLGLGYNPNVNEKDIRRATVIHFNGNLK 276
T PLD+SWH LGLGYNP+++ ++IR A V+HFNGN+K
Sbjct: 406 TKPLDKSWHVLGLGYNPSISMEEIRNAAVVHFNGNMK 516
Score = 50.1 bits (103), Expect(3) = 5e-030
Identities = 19/29 (65%), Positives = 23/29 (79%)
Frame = +2 / +1
Query: 89 QKLNENRLLWKLGTLPAGLVTFWNRIRSL 175
Q NENRLLWKLGTLP GL+TF++ + L
Sbjct: 331 QTQNENRLLWKLGTLPPGLITFYSTTKPL 417
Score = 43.2 bits (88), Expect(3) = 5e-030
Identities = 14/39 (35%), Positives = 24/39 (61%)
Frame = +2 / +1
Query: 281 WLEIGLPKYRKYWSAHVNYDQVFLQECNINP*TANSGVC 397
WL+IG+ ++R W+ +V+Y F+++CN P S C
Sbjct: 520 WLDIGMNQFRHLWTKYVDYGDSFIRQCNFAPP*KGSRCC 636
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 8,024,336
Number of Sequences: 8143
Number of extensions: 112861
Number of successful extensions: 1056
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 229164947
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)