TBLASTX 2.2.10 [Oct-19-2004]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= 3023930.2.1
         (544 letters)

Database: Avena_nucl_with_EST.fasta 
           8143 sequences; 4,702,463 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|CN815151.1|CN815151  HRO4506_D10_G20ZS5 Lib AA071E1X Aven...   100   3e-023
gb|CN818949.1|CN818949  HRO4474_G09_N18ZS5 Lib AA070E1X Aven...    98   9e-022

>gb|CN815151.1|CN815151 HRO4506_D10_G20ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
           sativa cDNA clone HRO4506_D10_G20, mRNA sequence
          Length = 599

 Score = 99.6 bits (211), Expect(2) = 3e-023
 Identities = 42/90 (46%), Positives = 59/90 (65%)
 Frame = +1 / +1

                                                                       
Query: 139 STYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
           +++LR+K+VNFG+  V L IS   L   +   GS  TVLTSS+ +DENSF  P KV PV+
Sbjct: 46  NSFLRVKIVNFGSDQVSLTISATGLQASINTLGSNATVLTSSNVMDENSFSNPNKVVPVT 225

                                         
Query: 319 SPMANAGQQMGASVGPYSLTSFDLLLEPSK 408
           S + NA +QM  ++  +S +SFDL L  S+
Sbjct: 226 SQLRNAAEQMQVTLPAHSFSSFDLALAQSQ 315




 Score = 23.1 bits (44), Expect(2) = 3e-023
 Identities = 9/20 (45%), Positives = 14/20 (70%)
 Frame = +1 / +1

                               
Query: 97  LVASAITWQNAKDKSTYLRI 156
           L ASAITWQ++ +    ++I
Sbjct: 10  LAASAITWQDSGNSFLRVKI 69



>gb|CN818949.1|CN818949 HRO4474_G09_N18ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
           Avena sativa cDNA clone HRO4474_G09_N18, mRNA sequence
          Length = 495

 Score = 98.2 bits (208), Expect = 9e-022
 Identities = 41/90 (45%), Positives = 59/90 (65%)
 Frame = +1 / +3

                                                                       
Query: 139 STYLRIKVVNFGNQAVDLNISVAELATGVKKSGSKQTVLTSSSPLDENSFQQPEKVAPVS 318
           +++LR+K+VNFG+  V L I+   L   +   GS  TVLTSS+ +DENSF  P KV PV+
Sbjct: 12  NSFLRVKIVNFGSDQVSLTITATGLQASINALGSNATVLTSSNVMDENSFSNPNKVVPVT 191

                                         
Query: 319 SPMANAGQQMGASVGPYSLTSFDLLLEPSK 408
           S + NA +QM  ++  +S +SFDL L  S+
Sbjct: 192 SQLRNAAEQMQVTLPAHSFSSFDLALAQSQ 281



  Database: Avena_nucl_with_EST.fasta
    Posted date:  Feb 7, 2006  2:15 PM
  Number of letters in database: 4,702,463
  Number of sequences in database:  8143
  
Lambda     K      H
   0.318    0.134    0.401 


Matrix: BLOSUM62
Number of Hits to DB: 6,490,393
Number of Sequences: 8143
Number of extensions: 88875
Number of successful extensions: 1827
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 169116378
frameshift window, decay const: 40,  0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)