TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3023309.2.1
(1096 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815937.1|CN815937 HRO4515_B04_D07ZS5 Lib AA071E1X Aven... 206 1e-082
gb|CK780282.1|CK780282 umn815 UMN cDNA from oat Avena sativ... 192 9e-051
>gb|CN815937.1|CN815937 HRO4515_B04_D07ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_B04_D07, mRNA sequence
Length = 809
Score = 206 bits (444), Expect(3) = 1e-082
Identities = 85/97 (87%), Positives = 90/97 (92%)
Frame = +2 / +1
Query: 185 SLTAVFSQFGKILDVLAFKTLKHKGQAWVVFEDVASATEALKRMQGFPFYDKTMRIQYAK 364
SL AVFSQFGKIL+VLAFKTLKHKGQAWVVFEDVASAT+ALK MQ FPFYDK M+IQYAK
Sbjct: 22 SLNAVFSQFGKILEVLAFKTLKHKGQAWVVFEDVASATKALKGMQDFPFYDKPMKIQYAK 201
Query: 365 TKSDIIAKADGTFVPRERRKRTDEKSEKKQKREHHHD 475
TKSD++AKADGTFVPRERRKR DEK EKKQKRE HHD
Sbjct: 202 TKSDVVAKADGTFVPRERRKRNDEKPEKKQKREQHHD 312
Score = 192 bits (414), Expect(2) = 6e-058
Identities = 81/130 (62%), Positives = 93/130 (71%)
Frame = +2 / +1
Query: 419 RKRTDEKSEKKQKREHHHDAGASQIGMGVTAYPGVYGAPQLTQIPIAGGQRVMMPEIIVP 598
R+R EK +K++ G+ AYPG YGAP L+Q+P G RVMMPEIIVP
Sbjct: 247 RERRKRNDEKPEKKQKREQHHDVSQAGGMNAYPGTYGAPPLSQLPFGAGPRVMMPEIIVP 426
Query: 599 NNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEAKPGIAFVEYGDETQATAAMNNLQS 778
N+ILFVQNLPH+TT M+LQM FCQY GFKEVR IE KPGIAFVEYGDE QATAAMN LQ
Sbjct: 427 NSILFVQNLPHDTTAMVLQMFFCQYQGFKEVRTIETKPGIAFVEYGDEGQATAAMNALQG 606
Query: 779 FKITKENQMV 808
FKI + +V
Sbjct: 607 FKIKETPMLV 636
Score = 158 bits (340), Expect(4) = 1e-081
Identities = 70/104 (67%), Positives = 78/104 (75%)
Frame = -1 / -3
Query: 478 SIMVMLALLLLFRFFISPLSSFSRYKGAISLCYNI*FGLGILNSHSFVIEGKALHPLQSL 299
+I+V+L LLLLFRFFI PL SFSRY+GAIS CYN+* GLGILN H FVIEGK LHPLQSL
Sbjct: 315 NIVVLLTLLLLFRFFIIPLPSFSRYEGAISFCYNV*LGLGILNFHRFVIEGKILHPLQSL 136
Query: 298 SS*CDIFEDNPSLTFMLQSLESKHIKNFPXXXXXXXXXXXKFFE 167
*C+IF+ NP L FMLQSL+SKH KNF KFFE
Sbjct: 135 GC*CNIFKHNPCLPFMLQSLKSKHFKNFAKLRENGVEGLLKFFE 4
Score = 158 bits (339), Expect(3) = 6e-048
Identities = 74/102 (72%), Positives = 80/102 (78%)
Frame = +3 / +2
Query: 171 KNLRSP*QPFFPSLGKFLMCLLSRL*SIKVKLGLSSKMSHQLLRL*RGCRAFPSMTKL*E 350
KNLRSP PF SL KFL CLL RL*SIK + GL KM HQ RL*RGC+ FPSMT L*+
Sbjct: 8 KNLRSPSTPFSLSLAKFLKCLLLRL*SIKGRHGLCLKMLHQQPRL*RGCKIFPSMTNL*K 187
Query: 351 FNMPRPNQIL*QRLMAPLYLEKEERGLMKNLKRSRSASITMM 476
FNMPRP+Q L*Q+LMAP YLEKE RG+MKNLKRSRS S T M
Sbjct: 188 FNMPRPSQTL*QKLMAPSYLEKEGRGMMKNLKRSRSVSSTTM 313
Score = 150 bits (323), Expect(4) = 1e-081
Identities = 69/97 (71%), Positives = 74/97 (76%)
Frame = -2 / -1
Query: 789 VILKL*RLFMAAVAWVSSPYSTKAIPGFASIIRTSLKPGYWQKSIWSIMGVVSCGKFWTN 610
+ILK *R FMAAVA SSPYSTKAIPGF SI+ TSL P YWQK+I M VVSCG+FWTN
Sbjct: 617 LILKP*RAFMAAVACPSSPYSTKAIPGFVSIVLTSLNPWYWQKNICRTMAVVSCGRFWTN 438
Query: 609 KMLFGTIISGIMTLCPPAIGIWVSCGAP*TPG*AVTP 499
+MLFGTIISGIMTL P G S GAP* PG*A P
Sbjct: 437 RMLFGTIISGIMTLGPAPKGS*ESGGAP*VPG*AFMP 327
Score = 138 bits (296), Expect(2) = 4e-067
Identities = 58/117 (49%), Positives = 78/117 (66%)
Frame = -1 / -3
Query: 793 FCNFEALKVVHGSSGLGLVSILHESNPRLRLDHPNLLEARVLAEEHLEHHGRRLVWQVLD 614
F +FEALK VHG L LVSILHES+ RL D P+ LE VLAEEHL++HG R+VW++LD
Sbjct: 621 FFDFEALKSVHGCGRLPLVSILHESDSRLCFDRPHFLEPLVLAEEHLQNHGGRVVWEILD 442
Query: 613 KQDVVWYDNLWHHDPLSSCYRYLGELWSPINTRVSSYTHANLTSTSIMVMLALLLLF 443
+Q+ VW++NLWHHDP R L + SPI+TR+ + + ++ +L L F
Sbjct: 441 EQNAVWHNNLWHHDPRPRTKR*LRKRRSPISTRIGIHATSLANIVVLLTLLLLFRFF 271
Score = 132 bits (282), Expect(2) = 4e-067
Identities = 62/76 (81%), Positives = 64/76 (84%)
Frame = -2 / -1
Query: 411 RGTKVPSAFAIISDLVLAY*ILIVLS*KGKPCILFRASVADATSSKTTQA*PLCFKVLKA 232
RGTKVPSAFA SDLVLAY*I I LS*KGK CI F+A VADATSS TT A PLCFKVLKA
Sbjct: 248 RGTKVPSAFATTSDLVLAY*IFIGLS*KGKSCIPFKALVADATSSNTTHACPLCFKVLKA 69
Query: 231 STSRIFPNWEKTAVRD 184
STSRI PN EKTA+RD
Sbjct: 68 STSRILPN*EKTALRD 21
Score = 127 bits (273), Expect(3) = 1e-044
Identities = 62/103 (60%), Positives = 68/103 (66%)
Frame = -3 / -2
Query: 476 HHGDARXXXXXXXXXXXXXXFLEVQRCHQPLL*YLIWSWHIEFS*FCHRRESPASSSEPQ 297
H G A FLEV+RCHQ LL L WSWHIEFS* CHRRE+ AS S+P
Sbjct: 313 HRGAAHASASFQVFHHSSSFFLEVRRCHQLLLQRLTWSWHIEFS*VCHRRENLASPSKPW 134
Query: 296 *LMRHLRRQPKLDLYASKS*KQAHQEFSQTGKKRLLGTS*VLR 168
LM+HL+ QP LYASKS*KQA QEF QT +KR GTS*VLR
Sbjct: 133 LLMQHLQTQPMPALYASKS*KQALQEFCQTERKRR*GTS*VLR 5
Score = 111 bits (237), Expect(3) = 1e-082
Identities = 48/101 (47%), Positives = 56/101 (55%)
Frame = +1 / +3
Query: 508 CLPWCLWGSTAHPDTYSRRTKGHDARDYRTKQHLVCPKLATRXXXXXXXXXXXXVPWLQG 687
CL W LWGS+A T+ +GHDARDY KQH V PK TR VP +QG
Sbjct: 336 CLSWYLWGSSAFSATFWCGAEGHDARDYCAKQHSVRPKSPTRHDRHGSADVLLPVPRVQG 515
Query: 688 GSDDRGEAWDCFRGVWRRDPGHCCHEQPSKLQNYKRKPDGH 810
DDR +AW+ FRGVWRR G HE+ S LQN + H
Sbjct: 516 SEDDRNKAWNRFRGVWRRGAGDRSHERSSGLQNQRNSDACH 638
Score = 50.6 bits (104), Expect(2) = 6e-058
Identities = 21/28 (75%), Positives = 22/28 (78%)
Frame = +1 / +3
Query: 169 RRT*EVPNSRFFPVWENS*CACFQDFEA 252
RRT*EVP RF VW+NS* ACF DFEA
Sbjct: 6 RRT*EVPQRRFLSVWQNS*SACF*DFEA 89
Score = 43.7 bits (89), Expect(3) = 1e-044
Identities = 23/38 (60%), Positives = 26/38 (68%)
Frame = -3 / -2
Query: 791 L*F*SFEGCSWQQWPGSRLHTPRKQSQASPRSSEPP*S 678
L*F*S E SW + P RLHTPRK+ QA RSS P*+
Sbjct: 619 L*F*SPEERSWLRSPAPRLHTPRKRFQALFRSSSLP*T 506
Score = 42.3 bits (86), Expect(3) = 1e-044
Identities = 20/37 (54%), Positives = 25/37 (67%)
Frame = -3 / -2
Query: 620 FGQTRCCLVR*SLAS*PFVLLL*VSG*AVEPHKHQGK 510
FG+T CCL +*SLAS*P V+ A EPHK+Q +
Sbjct: 448 FGRTECCLAQ*SLAS*PSAPHQKVAEKAEEPHKYQDR 338
Score = 34.1 bits (68), Expect(3) = 6e-048
Identities = 15/23 (65%), Positives = 17/23 (73%)
Frame = +3 / +2
Query: 726 WSMETRPRPLLP*TTFKASKLQK 794
WSMETR R P*T F+ASK +K
Sbjct: 554 WSMETRGRRPQP*TLFRASKSKK 622
Score = 32.7 bits (65), Expect(3) = 6e-048
Identities = 17/32 (53%), Positives = 18/32 (56%)
Frame = +3 / +2
Query: 525 MGLHSSPRYL*QEDKGS*CQRLSYQTTSCLSK 620
MGL YL +GS*CQRL QT C SK
Sbjct: 353 MGLLRFLSYLLVRGRGS*CQRLLCQTAFCSSK 448
Score = 24.0 bits (46), Expect(4) = 1e-081
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -2 / -1
Query: 900 EIYQLCKTRNTTSHTSGKH 844
+IYQLCK + T +S H
Sbjct: 707 KIYQLCKKQTTKLKSSVNH 651
Score = 23.5 bits (45), Expect(3) = 1e-082
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +2 / +1
Query: 791 KENQMVITYAKK* 829
KE M++TYAKK*
Sbjct: 616 KETPMLVTYAKK* 654
Score = 23.1 bits (44), Expect(4) = 1e-081
Identities = 8/19 (42%), Positives = 13/19 (68%)
Frame = -2 / -1
Query: 840 SGAHYFFAYVMTIWFSFVI 784
S ++FFAYV +I S ++
Sbjct: 665 SSVNHFFAYVTSIGVSLIL 609
>gb|CK780282.1|CK780282 umn815 UMN cDNA from oat Avena sativa cDNA clone JMT15 5', mRNA
sequence
Length = 555
Score = 192 bits (414), Expect(2) = 9e-051
Identities = 81/130 (62%), Positives = 93/130 (71%)
Frame = +2 / -3
Query: 419 RKRTDEKSEKKQKREHHHDAGASQIGMGVTAYPGVYGAPQLTQIPIAGGQRVMMPEIIVP 598
R+R EK +K++ G+ AYPG YGAP L+Q+P G RVMMPEIIVP
Sbjct: 553 RERRKRNDEKPEKKQKREQHHDVSQAGGMNAYPGTYGAPPLSQLPFGAGPRVMMPEIIVP 374
Query: 599 NNILFVQNLPHETTPMMLQMLFCQYPGFKEVRMIEAKPGIAFVEYGDETQATAAMNNLQS 778
N+ILFVQNLPH+TT M+LQM FCQY GFKEVR IE KPGIAFVEYGDE QATAAMN LQ
Sbjct: 373 NSILFVQNLPHDTTAMVLQMFFCQYQGFKEVRTIETKPGIAFVEYGDEGQATAAMNALQG 194
Query: 779 FKITKENQMV 808
FKI + +V
Sbjct: 193 FKIKETPMLV 164
Score = 150 bits (323), Expect(4) = 2e-046
Identities = 69/97 (71%), Positives = 74/97 (76%)
Frame = -2 / +3
Query: 789 VILKL*RLFMAAVAWVSSPYSTKAIPGFASIIRTSLKPGYWQKSIWSIMGVVSCGKFWTN 610
+ILK *R FMAAVA SSPYSTKAIPGF SI+ TSL P YWQK+I M VVSCG+FWTN
Sbjct: 183 LILKP*RAFMAAVACPSSPYSTKAIPGFVSIVLTSLNPWYWQKNICRTMAVVSCGRFWTN 362
Query: 609 KMLFGTIISGIMTLCPPAIGIWVSCGAP*TPG*AVTP 499
+MLFGTIISGIMTL P G S GAP* PG*A P
Sbjct: 363 RMLFGTIISGIMTLGPAPKGS*ESGGAP*VPG*AFMP 473
Score = 138 bits (296), Expect = 2e-033
Identities = 58/117 (49%), Positives = 78/117 (66%)
Frame = -1 / +2
Query: 793 FCNFEALKVVHGSSGLGLVSILHESNPRLRLDHPNLLEARVLAEEHLEHHGRRLVWQVLD 614
F +FEALK VHG L LVSILHES+ RL D P+ LE VLAEEHL++HG R+VW++LD
Sbjct: 179 FFDFEALKSVHGCGRLPLVSILHESDSRLCFDRPHFLEPLVLAEEHLQNHGGRVVWEILD 358
Query: 613 KQDVVWYDNLWHHDPLSSCYRYLGELWSPINTRVSSYTHANLTSTSIMVMLALLLLF 443
+Q+ VW++NLWHHDP R L + SPI+TR+ + + ++ +L L F
Sbjct: 359 EQNAVWHNNLWHHDPRPRTKR*LRKRRSPISTRIGIHATSLANIVVLLTLLLLFRFF 529
Score = 111 bits (237), Expect(2) = 2e-034
Identities = 48/101 (47%), Positives = 56/101 (55%)
Frame = +1 / -2
Query: 508 CLPWCLWGSTAHPDTYSRRTKGHDARDYRTKQHLVCPKLATRXXXXXXXXXXXXVPWLQG 687
CL W LWGS+A T+ +GHDARDY KQH V PK TR VP +QG
Sbjct: 464 CLSWYLWGSSAFSATFWCGAEGHDARDYCAKQHSVRPKSPTRHDRHGSADVLLPVPRVQG 285
Query: 688 GSDDRGEAWDCFRGVWRRDPGHCCHEQPSKLQNYKRKPDGH 810
DDR +AW+ FRGVWRR G HE+ S LQN + H
Sbjct: 284 SEDDRNKAWNRFRGVWRRGAGDRSHERSSGLQNQRNSDACH 162
Score = 49.6 bits (102), Expect(2) = 2e-034
Identities = 19/22 (86%), Positives = 19/22 (86%)
Frame = +2 / -3
Query: 410 RERRKRTDEKSEKKQKREHHHD 475
RERRKR DEK EKKQKRE HHD
Sbjct: 553 RERRKRNDEKPEKKQKREQHHD 488
Score = 40.0 bits (81), Expect(4) = 2e-046
Identities = 17/23 (73%), Positives = 20/23 (86%)
Frame = -1 / +2
Query: 478 SIMVMLALLLLFRFFISPLSSFS 410
+I+V+L LLLLFRFFI PL SFS
Sbjct: 485 NIVVLLTLLLLFRFFIIPLPSFS 553
Score = 24.0 bits (46), Expect(4) = 2e-046
Identities = 9/19 (47%), Positives = 12/19 (63%)
Frame = -2 / +3
Query: 900 EIYQLCKTRNTTSHTSGKH 844
+IYQLCK + T +S H
Sbjct: 93 KIYQLCKKQTTKLKSSVNH 149
Score = 23.5 bits (45), Expect(2) = 9e-051
Identities = 9/13 (69%), Positives = 11/13 (84%)
Frame = +2 / -3
Query: 791 KENQMVITYAKK* 829
KE M++TYAKK*
Sbjct: 184 KETPMLVTYAKK* 146
Score = 23.1 bits (44), Expect(4) = 2e-046
Identities = 8/19 (42%), Positives = 13/19 (68%)
Frame = -2 / +3
Query: 840 SGAHYFFAYVMTIWFSFVI 784
S ++FFAYV +I S ++
Sbjct: 135 SSVNHFFAYVTSIGVSLIL 191
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 10,980,257
Number of Sequences: 8143
Number of extensions: 157531
Number of successful extensions: 921
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 386942400
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)