TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3012883.2.1
(1189 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819097.1|CN819097 HRO4468_H12_O24ZS5 Lib AA070E1X Aven... 267 2e-072
gb|CN816561.1|CN816561 HRO4522_B07_C14ZS5 Lib AA071E1X Aven... 166 7e-042
gb|CN820670.1|CN820670 HRO4416_A10_B20ZS5 Lib AA069E1X Aven... 151 2e-037
>gb|CN819097.1|CN819097 HRO4468_H12_O24ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4468_H12_O24, mRNA sequence
Length = 808
Score = 267 bits (578), Expect = 2e-072
Identities = 112/206 (54%), Positives = 146/206 (70%)
Frame = +1 / +1
Query: 388 KTKVDELERDNLSNRQKPGCGKGSAVDRSREQTTGAVKKKLKERMDDFQTLREAIRQXXX 567
K K+++++R+NL NR+KPGCGKG++VDRSR T A+KKKLKERM+DFQ LR+ I+Q
Sbjct: 19 KVKLEQMDRNNLDNRKKPGCGKGTSVDRSRMSMTIALKKKLKERMNDFQNLRQTIQQEYR 198
Query: 568 XXXXXXXFTVTGNRPDEETIDDLIETGKSEQIFKDAIQHQGRGQILDTVAEIQERHDAVR 747
FTVTG +P +E ID+LIETG SEQIF+ AIQ GRGQ++ TV EIQERHD V
Sbjct: 199 EVVERRIFTVTGTKPSDEVIDNLIETGSSEQIFEKAIQGIGRGQVMATVQEIQERHDVVM 378
Query: 748 DLERKLLELQQIFMDMAVLVEAQGDMINNIETHVSNATNHIQQGVSALQNAKKLQRNSRK 927
D+ERKL+ELQQIF+DMA LV+ QG++++NIE+ V NA NH+Q G AL++AK +Q+ SRK
Sbjct: 379 DIERKLMELQQIFLDMAALVDTQGEILDNIESQVQNAVNHVQIGNEALRSAKNMQKKSRK 558
Query: 928 WMCYXXXXXXXXXXXXXXXXXQPWKK 1005
M +PW K
Sbjct: 559 CMMIAIILLLVIAGITVLAVLKPWAK 636
Score = 202 bits (435), Expect = 1e-052
Identities = 97/180 (53%), Positives = 107/180 (59%)
Frame = -3 / -3
Query: 926 FLEFLCSFFAFWSALTPCCMWLVAFETWVSMLLIMSPCASTRTAMSMNICCNSRSFLSRS 747
FL+F C F A A P C W AF TW+SML +SPC STR AMS ICCNS SFLS S
Sbjct: 557 FLDFFCMFLALRRASLPICTWFTAF*TWLSMLSNISPCVSTRAAMSKKICCNSISFLSIS 378
Query: 746 LTASCRSCISATVSSIWPLP*CWIASLNICSLFPXXXXXXXXXXSGRLPVTVKTLLSTTS 567
+T SCRS IS TV+ P P IA ICSL P G +PVTV LLSTTS
Sbjct: 377 MTTSCRS*IS*TVAITCPRPIP*IAFSKICSLLPVSIRLSITSSEGFVPVTVNILLSTTS 198
Query: 566 RYSCLIASLKV*KSSIRSFNFFFTAPVVCSLDRSTAEPFPHPGFCLFDRLSLSSSSTFVF 387
RYSC I L+ KS I S +FF A V+ LDRST PFP PGF L RL LS S+ F
Sbjct: 197 RYSCWIVCLRFWKSFILSLSFFLRAMVIDILDRSTLVPFPQPGFFLLSRLFLSICSSLTF 18
>gb|CN816561.1|CN816561 HRO4522_B07_C14ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4522_B07_C14, mRNA sequence
Length = 883
Score = 166 bits (357), Expect = 7e-042
Identities = 70/92 (76%), Positives = 73/92 (79%)
Frame = +1 / +1
Query: 730 RHDAVRDLERKLLELQQIFMDMAVLVEAQGDMINNIETHVSNATNHIQQGVSALQNAKKL 909
RHDAVRDLERKLLELQQIF+DMAVLV+AQGDMIN+IETHVSNATNHI QGV ALQNAKKL
Sbjct: 484 RHDAVRDLERKLLELQQIFLDMAVLVDAQGDMINHIETHVSNATNHISQGVGALQNAKKL 663
Query: 910 QRNSRKWMCYXXXXXXXXXXXXXXXXXQPWKK 1005
QRNSRKWMCY QPWKK
Sbjct: 664 QRNSRKWMCYAIILLLVVVVIIVVAVIQPWKK 759
Score = 143 bits (306), Expect = 7e-035
Identities = 62/92 (67%), Positives = 64/92 (69%)
Frame = -3 / -3
Query: 1004 FFHGWXXXXXXXXXXXXXXXXXXXXHFLEFLCSFFAFWSALTPCCMWLVAFETWVSMLLI 825
FFHGW HFLEFLCSFFAFW A TPC MWLVAFET VSM LI
Sbjct: 758 FFHGWITATTMITTTTRRRMMA*HIHFLEFLCSFFAFWRAPTPCDMWLVAFETCVSMWLI 579
Query: 824 MSPCASTRTAMSMNICCNSRSFLSRSLTASCR 729
MSP AST TA+S NICC+SRSFLSRSLTASCR
Sbjct: 578 MSP*ASTNTAISRNICCSSRSFLSRSLTASCR 483
Score = 100 bits (214), Expect = 3e-022
Identities = 43/71 (60%), Positives = 50/71 (70%)
Frame = -2 / -2
Query: 942 GVAHPLPRIPLQFLRVLERAHTLLYVVGGVRDMGLDVVDHVSLRLNKNCHVHEYXXXXXX 763
GVAHPL R+PLQ L VLE AH L YVVG + +M LDVVDHVSL +N++CH+ EY
Sbjct: 696 GVAHPLSRVPLQLLCVLEGAHALRYVVGSI*NMCLDVVDHVSLSINQHCHIQEYLLQLQE 517
Query: 762 XXX*IPYSIMS 730
IPYSIMS
Sbjct: 516 LPLQIPYSIMS 484
Score = 75.8 bits (159), Expect(2) = 5e-021
Identities = 31/47 (65%), Positives = 35/47 (74%)
Frame = +3 / +3
Query: 729 AT*CCKGSREEASGVATDIHGHGSSC*GARRHDQQHRDPCLERHQPH 869
AT*CCKGS EEA G A DI G+GS * +RRHDQ HRD C + +QPH
Sbjct: 483 AT*CCKGSGEEAPGAAADIPGYGSVG*CSRRHDQPHRDTCFKCYQPH 623
Score = 40.5 bits (82), Expect(2) = 5e-021
Identities = 17/26 (65%), Positives = 19/26 (73%)
Frame = +3 / +3
Query: 927 VDVLRHHHTAGDSRGHCRRSHPAMEE 1004
VDVLRHH + S H RRS+PAMEE
Sbjct: 681 VDVLRHHPSPRGSGDHRRRSYPAMEE 758
>gb|CN820670.1|CN820670 HRO4416_A10_B20ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4416_A10_B20, mRNA
sequence
Length = 564
Score = 151 bits (325), Expect = 2e-037
Identities = 64/86 (74%), Positives = 67/86 (77%)
Frame = +1 / +1
Query: 748 DLERKLLELQQIFMDMAVLVEAQGDMINNIETHVSNATNHIQQGVSALQNAKKLQRNSRK 927
DLERKLLELQQIF+DMAVLV+AQGDMIN+IETHVSNATNHI QGV ALQNAKKLQRNSRK
Sbjct: 13 DLERKLLELQQIFLDMAVLVDAQGDMINHIETHVSNATNHISQGVGALQNAKKLQRNSRK 192
Query: 928 WMCYXXXXXXXXXXXXXXXXXQPWKK 1005
WMCY QPWKK
Sbjct: 193 WMCYAIILLLVVVVIIVVAVIQPWKK 270
Score = 128 bits (275), Expect = 1e-030
Identities = 56/86 (65%), Positives = 58/86 (67%)
Frame = -3 / -2
Query: 1004 FFHGWXXXXXXXXXXXXXXXXXXXXHFLEFLCSFFAFWSALTPCCMWLVAFETWVSMLLI 825
FFHGW HFLEFLCSFFAFW A TPC MWLVAFET VSM LI
Sbjct: 269 FFHGWITATTMITTTTRRRMMA*HIHFLEFLCSFFAFWRAPTPCDMWLVAFETCVSMWLI 90
Query: 824 MSPCASTRTAMSMNICCNSRSFLSRS 747
MSP AST TA+S NICC+SRSFLSRS
Sbjct: 89 MSP*ASTNTAISRNICCSSRSFLSRS 12
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 12,463,512
Number of Sequences: 8143
Number of extensions: 176746
Number of successful extensions: 1175
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 424427445
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)