TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 3012873.2.2
(1520 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819392.1|CN819392 HRO4405_E01_I01ZS5 Lib AA069E1X Aven... 87 5e-043
gb|CN820523.1|CN820523 HRO4413_D02_G03ZS5 Lib AA069E1X Aven... 80 7e-027
gb|CN815837.1|CN815837 HRO4511_A06_B11ZS5 Lib AA071E1X Aven... 112 2e-025
>gb|CN819392.1|CN819392 HRO4405_E01_I01ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4405_E01_I01, mRNA
sequence
Length = 717
Score = 86.8 bits (183), Expect(3) = 5e-043
Identities = 33/84 (39%), Positives = 55/84 (65%)
Frame = +3 / +1
Query: 858 QATVNASIAHILKYLDGSARTFANAVQAYVDVRDVADAHLRVFESPRASGRHLCAERVLH 1037
Q T+N S ILK ++GS+ T+ N +++V+DVA AH+ +E P A+GR+ ERV H
Sbjct: 445 QPTLNTSAEAILKLINGSSSTYPNFSFGWINVKDVALAHILAYEVPSANGRYCMVERVAH 624
Query: 1038 REDVVRILAKLFPEYPVPARCSDE 1109
++V + K++P+ PV +C+D+
Sbjct: 625 HSELVNFIHKMYPDIPVAHKCADD 696
Score = 71.6 bits (150), Expect(3) = 5e-043
Identities = 31/87 (35%), Positives = 49/87 (56%)
Frame = +3 / +1
Query: 540 VTDDPEQMVEPAVRGTEYVINAAAEAGTVRRVVFTSSIGAVTMDPKRGPDVVVDESCWSD 719
V D ++++PAV GT V+ + +A R +V +S + P+VVVDE+ +S
Sbjct: 127 VKDPKAELLDPAVNGTLNVLRSCKKASIKRVIVTSSMAAVGYNGKPKTPNVVVDETWFSH 306
Query: 720 LEFCEKTRNWYCYGKAVAEHAAWETAR 800
E CEK + WY K +AE AAW+ ++
Sbjct: 307 AELCEKGQQWYVLSKTLAEEAAWKFSK 387
Score = 50.6 bits (104), Expect(3) = 5e-043
Identities = 22/38 (57%), Positives = 27/38 (71%)
Frame = +3 / +1
Query: 426 ALDGAAERLILCKADLLDYDAICRAVQGCQGVFHTASP 539
ALDGA +RL L KA LL+ + A+ GC+ VFHTASP
Sbjct: 1 ALDGAKDRLHLFKASLLEEGSFDDAIDGCECVFHTASP 114
>gb|CN820523.1|CN820523 HRO4413_D02_G03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4413_D02_G03, mRNA
sequence
Length = 704
Score = 79.9 bits (168), Expect(2) = 7e-027
Identities = 34/78 (43%), Positives = 46/78 (58%)
Frame = +3 / +3
Query: 858 QATVNASIAHILKYLDGSARTFANAVQAYVDVRDVADAHLRVFESPRASGRHLCAERVLH 1037
Q+TVN S +L Y G T N + VDVRDVADA L +E+ ASGR++C+ +
Sbjct: 330 QSTVNTSSKVLLSYFKGEHETVENKYRNLVDVRDVADAILLAYENAEASGRYICSSSPIK 509
Query: 1038 REDVVRILAKLFPEYPVP 1091
D++ IL L+P YP P
Sbjct: 510 ASDLISILKTLYPTYPYP 563
Score = 56.5 bits (117), Expect(2) = 7e-027
Identities = 24/60 (40%), Positives = 34/60 (56%)
Frame = +3 / +3
Query: 624 VRRVVFTSSIGAVTMDPKRGPDVVVDESCWSDLEFCEKTRNWYCYGKAVAEHAAWETARR 803
V+RVV SS+ AV +P V+DE WSD + C K ++WY K +AE A+ A +
Sbjct: 96 VKRVVVVSSVSAVYNNPNWPKGKVLDEDNWSDEDLCRKNKDWYDLSKTLAEREAFAYAAK 275
>gb|CN815837.1|CN815837 HRO4511_A06_B11ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4511_A06_B11, mRNA sequence
Length = 741
Score = 112 bits (239), Expect = 2e-025
Identities = 44/51 (86%), Positives = 47/51 (92%)
Frame = +3 / +2
Query: 1029 VLHREDVVRILAKLFPEYPVPARCSDEVNPRKQPYKFSNQKLRDLGLQFRP 1181
VLHREDVVRIL+KLFPEYPVP RCSDE N RKQPYK SNQKL+DLGL+FRP
Sbjct: 587 VLHREDVVRILSKLFPEYPVPTRCSDEANSRKQPYKMSNQKLQDLGLEFRP 739
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,986,708
Number of Sequences: 8143
Number of extensions: 155465
Number of successful extensions: 1101
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 45
effective length of database: 1,201,052
effective search space used: 553684972
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)