TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2969865.2.1
(675 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818609.1|CN818609 HRO4467_C02_E03ZS5 Lib AA070E1X Aven... 184 1e-047
gb|CN818166.1|CN818166 HRO4471_C12_E23ZS5 Lib AA070E1X Aven... 176 3e-045
>gb|CN818609.1|CN818609 HRO4467_C02_E03ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4467_C02_E03, mRNA sequence
Length = 463
Score = 184 bits (397), Expect = 1e-047
Identities = 77/77 (100%), Positives = 77/77 (100%)
Frame = +3 / +2
Query: 177 TQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKM 356
TQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKM
Sbjct: 2 TQVLINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKM 181
Query: 357 FLRGDSVIIVLRNPK*V 407
FLRGDSVIIVLRNPK*V
Sbjct: 182 FLRGDSVIIVLRNPK*V 232
Score = 164 bits (353), Expect = 1e-041
Identities = 72/77 (93%), Positives = 72/77 (93%)
Frame = -3 / -2
Query: 406 THFGFLRTMMTESPRRNILLMNLSLFTGRAFFLPLPVFGTSVHISLTFSRTMLQCRSKAR 227
THFGFLRTMMTESPRRNILLMNLSLFTGRAFFLPLPVFGTSVHISLTFSRTMLQ RS A
Sbjct: 231 THFGFLRTMMTESPRRNILLMNLSLFTGRAFFLPLPVFGTSVHISLTFSRTMLQ*RSNAL 52
Query: 226 TRPSNFLLFRQLIRTWV 176
TRPSNFLLFRQLI T V
Sbjct: 51 TRPSNFLLFRQLISTCV 1
Score = 135 bits (290), Expect = 6e-033
Identities = 55/77 (71%), Positives = 63/77 (81%)
Frame = +2 / +1
Query: 176 YPGSYQLPEQQEVAWSRASLRSALQHGS*ER*GDVDRGTEDR*RQEEGSPGEQGQVHKQD 355
+ G+YQLPEQQEV W R +RS+LQHGS*ER*GDVD G +D RQEEGS EQGQVH+QD
Sbjct: 1 HTGAYQLPEQQEVTWPREGIRSSLQHGS*ER*GDVD*GPKDWQRQEEGSSSEQGQVHQQD 180
Query: 356 VPPWGFGHHCPQEPEMS 406
VPP G HHCPQEP++S
Sbjct: 181 VPPRGLCHHCPQEPKVS 231
Score = 127 bits (272), Expect = 2e-030
Identities = 57/75 (76%), Positives = 60/75 (80%)
Frame = -2 / -1
Query: 404 SFRVPEDNDDRIPTEEHLAYEPVLVHRESLLLAFTGLRYLGPHLPNVLKNHVAVPIEGSH 225
S VPEDNDDR+P EEHLA EPVLVH +SLLLAF L L PHLPNVLKNHVAV IE H
Sbjct: 229 SLWVPEDNDDRVPAEEHLADEPVLVHWKSLLLAFASLWDLSPHLPNVLKNHVAVTIECPH 50
Query: 224 ATKQLLVVPAVDKNL 180
A K LLVVPAVDK+L
Sbjct: 49 AAK*LLVVPAVDKHL 5
Score = 98.7 bits (209), Expect = 9e-022
Identities = 47/75 (62%), Positives = 49/75 (65%)
Frame = -1 / -3
Query: 405 LISGS*GQ**PNPHGGTSCL*TCPCSPGEPSSCLYXXXXXXXXXXXXSQEPCCSADRRLA 226
L GS*GQ** +P GGTSC *TCPCS EPSSCL SQEPCCS DR +
Sbjct: 230 LTLGS*GQ**QSPRGGTSC**TCPCSLEEPSSCLCQSLGPQSTSP*RSQEPCCSDDRMPS 51
Query: 225 RDQATSCCSGS**EP 181
R Q TSCCSGS** P
Sbjct: 50 RGQVTSCCSGS**AP 6
>gb|CN818166.1|CN818166 HRO4471_C12_E23ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4471_C12_E23, mRNA sequence
Length = 475
Score = 176 bits (379), Expect = 3e-045
Identities = 73/73 (100%), Positives = 73/73 (100%)
Frame = +3 / +2
Query: 186 LINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKMFLR 365
LINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKMFLR
Sbjct: 2 LINCRNNKKLLGRVRAFDRHCNMVLENVREMWTEVPKTGKGKKKALPVNKDRFISKMFLR 181
Query: 366 GDSVIIVLRNPK* 404
GDSVIIVLRNPK*
Sbjct: 182 GDSVIIVLRNPK* 220
Score = 158 bits (340), Expect = 8e-040
Identities = 68/72 (94%), Positives = 68/72 (94%)
Frame = -3 / -2
Query: 403 HFGFLRTMMTESPRRNILLMNLSLFTGRAFFLPLPVFGTSVHISLTFSRTMLQCRSKART 224
HFGFLRTMMTESPRRNILLMNLSLFTGRAFFLP PVFGTSVHISLTFSRTMLQ RS A T
Sbjct: 219 HFGFLRTMMTESPRRNILLMNLSLFTGRAFFLPFPVFGTSVHISLTFSRTMLQWRSNALT 40
Query: 223 RPSNFLLFRQLI 188
RPSNFLLFRQLI
Sbjct: 39 RPSNFLLFRQLI 4
Score = 128 bits (274), Expect = 1e-030
Identities = 53/74 (71%), Positives = 60/74 (81%)
Frame = +2 / +1
Query: 185 SYQLPEQQEVAWSRASLRSALQHGS*ER*GDVDRGTEDR*RQEEGSPGEQGQVHKQDVPP 364
+YQLPEQQEV W R + S LQHGS*ER*GDVDRG++D RQEEGS EQGQVH+QDVPP
Sbjct: 1 AYQLPEQQEVTWPREGI*SPLQHGS*ER*GDVDRGSKDWERQEEGSSCEQGQVHQQDVPP 180
Query: 365 WGFGHHCPQEPEMS 406
GHHC +EPE+S
Sbjct: 181 RRLGHHCSEEPEVS 222
Score = 126 bits (269), Expect = 5e-030
Identities = 55/73 (75%), Positives = 60/73 (82%)
Frame = -2 / -1
Query: 404 SFRVPEDNDDRIPTEEHLAYEPVLVHRESLLLAFTGLRYLGPHLPNVLKNHVAVPIEGSH 225
S RVP++NDDR+ EEHLA EPVLVHR+SLLLAF L LGPHLPNVLKNHVAV I+ H
Sbjct: 220 SLRVPQNNDDRVSAEEHLADEPVLVHRKSLLLAFPSLWNLGPHLPNVLKNHVAVAIKCPH 41
Query: 224 ATKQLLVVPAVDK 186
A K LLVVPAVDK
Sbjct: 40 AAK*LLVVPAVDK 2
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,548,409
Number of Sequences: 8143
Number of extensions: 108158
Number of successful extensions: 1195
Number of sequences better than 1.0e-020: 2
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 223037542
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)