TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950290.2.2
(867 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821309.1|CN821309 HRO4423_H04_P07ZS5 Lib AA069E1X Aven... 74 2e-037
>gb|CN821309.1|CN821309 HRO4423_H04_P07ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4423_H04_P07, mRNA
sequence
Length = 585
Score = 73.9 bits (155), Expect(3) = 2e-037
Identities = 31/37 (83%), Positives = 34/37 (91%)
Frame = -3 / +2
Query: 679 GPGQLFKALAVFALRDINLTKIESRPHKERPLRVSDD 569
G QLFKALAVFALR INLTK+ESRPHK+RPLRV+DD
Sbjct: 2 GTRQLFKALAVFALRKINLTKMESRPHKKRPLRVADD 112
Score = 69.3 bits (145), Expect(3) = 2e-037
Identities = 27/36 (75%), Positives = 31/36 (86%)
Frame = -3 / +2
Query: 574 DDCSSLLKNFDYLFYVDLEASMADPKTQNALGNLKE 467
D+CS+ LK+FDYLFYVD EASMADP QNAL NLK+
Sbjct: 110 DNCSTPLKHFDYLFYVDFEASMADPNAQNALNNLKQ 217
Score = 60.2 bits (125), Expect(3) = 2e-021
Identities = 27/34 (79%), Positives = 28/34 (82%)
Frame = +3 / -1
Query: 579 TRRGLSLCGRLSIFVRLMSLNANTASALKSCPGP 680
TRRGL LCGRLSI VRL+ L ANTASALKSC P
Sbjct: 102 TRRGLFLCGRLSILVRLIFLRANTASALKSCLVP 1
Score = 45.5 bits (93), Expect(3) = 2e-037
Identities = 19/22 (86%), Positives = 21/22 (95%)
Frame = -3 / +2
Query: 472 KEFATFLRVLGSYPTDVNEP*I 407
+EFATFLRVLGSYPTDV+E *I
Sbjct: 215 QEFATFLRVLGSYPTDVSET*I 280
Score = 40.0 bits (81), Expect(3) = 2e-021
Identities = 16/34 (47%), Positives = 23/34 (67%)
Frame = +1 / -2
Query: 472 SDFQEHSESLDQPLMLQGQHKKGNQSSLRARNSH 573
S + EH E LDQPL Q H++G++S+ A NS+
Sbjct: 212 SSYSEHFEHLDQPLTPQNLHRRGSRSASGAWNSY 111
Score = 34.5 bits (69), Expect(3) = 2e-021
Identities = 15/18 (83%), Positives = 16/18 (88%)
Frame = +3 / -1
Query: 417 SFTSVG*LPRTLKNVANS 470
S TSVG*LPRTL+ VANS
Sbjct: 270 SLTSVG*LPRTLRKVANS 217
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 9,549,558
Number of Sequences: 8143
Number of extensions: 128684
Number of successful extensions: 727
Number of sequences better than 1.0e-020: 1
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 298247810
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)