TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950160.2.1
(846 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Aven... 75 2e-041
gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Aven... 74 1e-039
gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Aven... 72 1e-026
>gb|CN821264.1|CN821264 HRO4423_D02_H03ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4423_D02_H03, mRNA
sequence
Length = 733
Score = 75.3 bits (158), Expect(3) = 2e-041
Identities = 24/48 (50%), Positives = 33/48 (68%)
Frame = -2 / +1
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++D + I AV HYNGNMKPWL++ M KY+ +W Y+ Y HP I+ C
Sbjct: 409 SIDRSEIDNAAVAHYNGNMKPWLELAMTKYRPYWTRYIKYDHPYIRGC 552
Score = 67.0 bits (140), Expect(3) = 2e-041
Identities = 24/35 (68%), Positives = 26/35 (74%)
Frame = -2 / +1
Query: 755 DHTLWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
D LWKLG+LPPGL+ FY L LD WHVLGLGY
Sbjct: 298 DRVLWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGY 402
Score = 60.2 bits (125), Expect(3) = 2e-041
Identities = 18/29 (62%), Positives = 24/29 (82%)
Frame = -3 / +1
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERN 758
WA+GMN+ DL +W+NK++TGIYH WQ N
Sbjct: 208 WAYGMNIFDLKQWKNKDITGIYHKWQNMN 294
>gb|CN815287.1|CN815287 HRO4508_A06_B12ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_A06_B12, mRNA sequence
Length = 570
Score = 73.9 bits (155), Expect(3) = 1e-039
Identities = 24/48 (50%), Positives = 33/48 (68%)
Frame = -2 / +1
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
TV+ + I AV+HYNGNMKPWL+I M KY+ +W Y++Y H + C
Sbjct: 298 TVEHSEIDSAAVIHYNGNMKPWLEIAMTKYRPYWTKYINYEHSYVHGC 441
Score = 64.3 bits (134), Expect(3) = 1e-039
Identities = 23/32 (71%), Positives = 25/32 (78%)
Frame = -2 / +1
Query: 746 LWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
LWKLG+LPPGL+ FY L LD WHVLGLGY
Sbjct: 196 LWKLGTLPPGLMTFYKLTHPLDKSWHVLGLGY 291
Score = 58.3 bits (121), Expect(3) = 1e-039
Identities = 21/38 (55%), Positives = 26/38 (68%)
Frame = -3 / +1
Query: 844 WAFGMNVLDLVEWRNKNVTGIYHYWQERNVIILYGSLG 731
WA+GMN+ DL EW+ K++TGIYH WQ N L LG
Sbjct: 97 WAYGMNMFDLEEWKKKDITGIYHRWQTLNENRLLWKLG 210
>gb|CN815958.1|CN815958 HRO4515_D01_H01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4515_D01_H01, mRNA sequence
Length = 653
Score = 71.6 bits (150), Expect(2) = 1e-026
Identities = 23/48 (47%), Positives = 33/48 (68%)
Frame = -2 / +1
Query: 647 TVDLATIKEGAVLHYNGNMKPWLKIGMEKYKSFWDNYVDYSHPLIQQC 504
++ + I+ AV+H+NGNMKPWL IGM +++ W YVDY I+QC
Sbjct: 457 SISMEEIRNAAVVHFNGNMKPWLDIGMNQFRHLWTKYVDYGDSFIRQC 600
Score = 63.4 bits (132), Expect(2) = 1e-026
Identities = 22/32 (68%), Positives = 25/32 (78%)
Frame = -2 / +1
Query: 746 LWKLGSLPPGLLAFYGLVEALDPKWHVLGLGY 651
LWKLG+LPPGL+ FY + LD WHVLGLGY
Sbjct: 355 LWKLGTLPPGLITFYSTTKPLDKSWHVLGLGY 450
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,395,147
Number of Sequences: 8143
Number of extensions: 178816
Number of successful extensions: 1107
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 289726444
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)