TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2950089.2.3
(824 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN819616.1|CN819616 HRO4402_B12_C24ZS5 Lib AA069E1X Aven... 279 5e-076
gb|CN821498.1|CN821498 HRO4426_A05_A10ZS5 Lib AA069E1X Aven... 279 5e-076
gb|CN818433.1|CN818433 HRO4460_F10_K20ZS5 Lib AA070E1X Aven... 271 1e-073
>gb|CN819616.1|CN819616 HRO4402_B12_C24ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4402_B12_C24, mRNA
sequence
Length = 769
Score = 279 bits (603), Expect = 5e-076
Identities = 109/149 (73%), Positives = 125/149 (83%)
Frame = -3 / +3
Query: 573 MGSGSFLKVLANNFDVLAGPLISLAYPLYASVRAIETKNPVDDQQWLTYWVXXSFITLFE 394
MGSGSFLKVL +N DVLAGP++SLAYPLYASVRAIETK+ +DDQQWLTYWV SFITLFE
Sbjct: 48 MGSGSFLKVLVSNLDVLAGPIVSLAYPLYASVRAIETKSSIDDQQWLTYWVLYSFITLFE 227
Query: 393 LTFAPIIEWLPFWSYAKLFFNCXLVLPWFNGXAYVYDHFXRPMFVNRQIVNIWYIXRNEK 214
LTFA +IEWLPFWSY KLFFNC LVLP+FNG A+VY+HF RPM VN+Q+VNIWYI + E
Sbjct: 228 LTFAAVIEWLPFWSYGKLFFNCWLVLPYFNGAAHVYEHFVRPMIVNQQVVNIWYIPKKED 407
Query: 213 LGKSDDVXXAAERYIEQNXXXAFXXLISK 127
K DDV AA++YIEQN AF L++K
Sbjct: 408 SDKPDDVISAAQKYIEQNGSKAFEALVNK 494
Score = 190 bits (410), Expect = 2e-049
Identities = 95/146 (65%), Positives = 102/146 (69%)
Frame = +2 / -1
Query: 122 VDLLMSXSNAXXXFCSIYLSAAXXTSSDLPSFSFLXIYHMXTIWRFTNIGRXKWS*T*AX 301
++LL SNA FCS+Y AA TSS L SFL IY M T FT IGR K S*T A
Sbjct: 499 LNLLTRASNAFDPFCSMYFCAAEITSSGLSESSFLGIYQMFTTCWFTIIGRTKCS*TCAA 320
Query: 302 PLNQGKTXXQLKNSFAYDQNGSHSIIGAKVSSNKVINEXXTQ*VSHC*SSTGFFVSIALT 481
PL GKT QLKNSF DQ GSHSI AKVSSN+VINE TQ VSHC*SS FVSIA T
Sbjct: 319 PLK*GKTNQQLKNSFP*DQKGSHSITAAKVSSNRVINEYNTQYVSHC*SSIEDFVSIART 140
Query: 482 EAYRG*ASDMSGPARTSKLLASTLRK 559
+AYRG*AS+ GPARTS+LL STLRK
Sbjct: 139 DAYRG*ASETMGPARTSRLLTSTLRK 62
Score = 151 bits (325), Expect = 1e-037
Identities = 79/150 (52%), Positives = 96/150 (64%)
Frame = +3 / -2
Query: 123 *TC**AXQMXXVXFVQYISLQLXXHHQIYLASHFXXYTICXLFGGSQTLAXXNGRKHRQX 302
*TC* QM + FV IS+QL H ++YL YT C L GSQ+LA N KH Q
Sbjct: 498 *TC*PEPQMLLIHFVLCISVQLKLHRRVYLNLPSWEYTRCLLLAGSQSLAAQNAHKHAQH 319
Query: 303 H*TKARPXSS*RTALHTTKMEATR*LEQKLAQTK**MSXTPSK*ATADHLQDFLSL*L*Q 482
H*++ARP S+*+TA H T+ EA L+Q+ AQT+** S TPS *A ADHL L L L Q
Sbjct: 318 H*SRARPTSN*KTAFHKTRREAILLLQQR*AQTE**TSTTPSM*AIADHL*KILFLLLEQ 139
Query: 483 KHIEDKQVI*VARQGHRSYWPAP*GKIRTP 572
HIEDK+V A + HR PAP*G+I+TP
Sbjct: 138 THIEDKRVKLWAPREHRGC*PAP*GRIQTP 49
Score = 144 bits (308), Expect(2) = 2e-038
Identities = 63/116 (54%), Positives = 77/116 (66%)
Frame = +1 / -3
Query: 124 RLADELXKCXXXVLFNISLCSXXNIIRFT*LLISXNIPYVXYLAVHKHWPXKMVVNIGXT 303
+L D+ KC +LF + LCS I+ F *+ + NIP V YL VH HWP KM++N+ T
Sbjct: 497 KLVDQSLKCF*SILFYVFLCS*NYIVGFI*IFLLGNIPDVYYLLVHNHWPHKMLINMRST 318
Query: 304 IEPRQDQXAVKEQLCIRPKWKPLDNWSKS*LKQSDK*VXHPVSEPLLIIYRIFCLY 471
IE QDQ A+++QL IRP+ KP SK LKQSDK V HPV EPLLIIYR FC Y
Sbjct: 317 IEVGQDQPAIEKQLSIRPEGKPFYYCSKGKLKQSDKRVQHPVCEPLLIIYRRFCFY 150
Score = 83.5 bits (176), Expect(2) = 1e-029
Identities = 41/72 (56%), Positives = 49/72 (68%)
Frame = -2 / +2
Query: 574 DGVRIFPQGAGQ*LRCPCRATHITCLSSICFC*SYRDKKSCR*SAVAHLLGVXLIYHFV* 395
+GV I PQGAGQ RC A T LSSIC C S R+K R*SA+AH+LGV L+YH V*
Sbjct: 47 NGVWILPQGAGQQPRCSRGAHSFTRLSSICVCSSNRNKIFYR*SAMAHILGVVLVYHSV* 226
Query: 394 ANFCSNYRVASI 359
A C + R+AS+
Sbjct: 227 AYLCCSNRMASL 262
Score = 61.6 bits (128), Expect(2) = 1e-029
Identities = 36/77 (46%), Positives = 42/77 (54%)
Frame = -1 / +1
Query: 350 MQSCSLTAXWSCLGSMVLPMFTTIXXGQCL*TAK*XTYGIXXEMRS*VNLMMXXXLQRDI 171
M+SC A WSC SMVL MF +I GQ L*T+K* T GI + +N M L R+
Sbjct: 271 MESCFSIAGWSCPTSMVLRMFMSILCGQ*L*TSK**TSGIFPRRKIQINPTM*FQLHRNT 450
Query: 170 LNKTXXXHLXSSSASLR 120
NK HL S SLR
Sbjct: 451 *NKMDQKHLRLWSTSLR 501
Score = 30.4 bits (60), Expect(2) = 2e-038
Identities = 12/19 (63%), Positives = 14/19 (73%)
Frame = +1 / -3
Query: 517 PGKDIEVIGQHLEERSGPH 573
P ++IEV QHLEE S PH
Sbjct: 104 PRENIEVADQHLEEGSRPH 48
>gb|CN821498.1|CN821498 HRO4426_A05_A10ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4426_A05_A10, mRNA
sequence
Length = 839
Score = 279 bits (603), Expect = 5e-076
Identities = 109/149 (73%), Positives = 125/149 (83%)
Frame = -3 / +3
Query: 573 MGSGSFLKVLANNFDVLAGPLISLAYPLYASVRAIETKNPVDDQQWLTYWVXXSFITLFE 394
MGSGSFLKVL +N DVLAGP++SLAYPLYASVRAIETK+ +DDQQWLTYWV SFITLFE
Sbjct: 42 MGSGSFLKVLVSNLDVLAGPIVSLAYPLYASVRAIETKSSIDDQQWLTYWVLYSFITLFE 221
Query: 393 LTFAPIIEWLPFWSYAKLFFNCXLVLPWFNGXAYVYDHFXRPMFVNRQIVNIWYIXRNEK 214
LTFA +IEWLPFWSY KLFFNC LVLP+FNG A+VY+HF RPM VN+Q+VNIWYI + E
Sbjct: 222 LTFAAVIEWLPFWSYGKLFFNCWLVLPYFNGAAHVYEHFVRPMIVNQQVVNIWYIPKKED 401
Query: 213 LGKSDDVXXAAERYIEQNXXXAFXXLISK 127
K DDV AA++YIEQN AF L++K
Sbjct: 402 SDKPDDVISAAQKYIEQNGSKAFEALVNK 488
Score = 190 bits (410), Expect = 2e-049
Identities = 95/146 (65%), Positives = 102/146 (69%)
Frame = +2 / -2
Query: 122 VDLLMSXSNAXXXFCSIYLSAAXXTSSDLPSFSFLXIYHMXTIWRFTNIGRXKWS*T*AX 301
++LL SNA FCS+Y AA TSS L SFL IY M T FT IGR K S*T A
Sbjct: 493 LNLLTRASNAFDPFCSMYFCAAEITSSGLSESSFLGIYQMFTTCWFTIIGRTKCS*TCAA 314
Query: 302 PLNQGKTXXQLKNSFAYDQNGSHSIIGAKVSSNKVINEXXTQ*VSHC*SSTGFFVSIALT 481
PL GKT QLKNSF DQ GSHSI AKVSSN+VINE TQ VSHC*SS FVSIA T
Sbjct: 313 PLK*GKTNQQLKNSFP*DQKGSHSITAAKVSSNRVINEYNTQYVSHC*SSIEDFVSIART 134
Query: 482 EAYRG*ASDMSGPARTSKLLASTLRK 559
+AYRG*AS+ GPARTS+LL STLRK
Sbjct: 133 DAYRG*ASETMGPARTSRLLTSTLRK 56
Score = 151 bits (325), Expect = 1e-037
Identities = 79/150 (52%), Positives = 96/150 (64%)
Frame = +3 / -3
Query: 123 *TC**AXQMXXVXFVQYISLQLXXHHQIYLASHFXXYTICXLFGGSQTLAXXNGRKHRQX 302
*TC* QM + FV IS+QL H ++YL YT C L GSQ+LA N KH Q
Sbjct: 492 *TC*PEPQMLLIHFVLCISVQLKLHRRVYLNLPSWEYTRCLLLAGSQSLAAQNAHKHAQH 313
Query: 303 H*TKARPXSS*RTALHTTKMEATR*LEQKLAQTK**MSXTPSK*ATADHLQDFLSL*L*Q 482
H*++ARP S+*+TA H T+ EA L+Q+ AQT+** S TPS *A ADHL L L L Q
Sbjct: 312 H*SRARPTSN*KTAFHKTRREAILLLQQR*AQTE**TSTTPSM*AIADHL*KILFLLLEQ 133
Query: 483 KHIEDKQVI*VARQGHRSYWPAP*GKIRTP 572
HIEDK+V A + HR PAP*G+I+TP
Sbjct: 132 THIEDKRVKLWAPREHRGC*PAP*GRIQTP 43
Score = 144 bits (308), Expect(2) = 1e-038
Identities = 63/116 (54%), Positives = 77/116 (66%)
Frame = +1 / -1
Query: 124 RLADELXKCXXXVLFNISLCSXXNIIRFT*LLISXNIPYVXYLAVHKHWPXKMVVNIGXT 303
+L D+ KC +LF + LCS I+ F *+ + NIP V YL VH HWP KM++N+ T
Sbjct: 491 KLVDQSLKCF*SILFYVFLCS*NYIVGFI*IFLLGNIPDVYYLLVHNHWPHKMLINMRST 312
Query: 304 IEPRQDQXAVKEQLCIRPKWKPLDNWSKS*LKQSDK*VXHPVSEPLLIIYRIFCLY 471
IE QDQ A+++QL IRP+ KP SK LKQSDK V HPV EPLLIIYR FC Y
Sbjct: 311 IEVGQDQPAIEKQLSIRPEGKPFYYCSKGKLKQSDKRVQHPVCEPLLIIYRRFCFY 144
Score = 83.5 bits (176), Expect(2) = 1e-029
Identities = 41/72 (56%), Positives = 49/72 (68%)
Frame = -2 / +2
Query: 574 DGVRIFPQGAGQ*LRCPCRATHITCLSSICFC*SYRDKKSCR*SAVAHLLGVXLIYHFV* 395
+GV I PQGAGQ RC A T LSSIC C S R+K R*SA+AH+LGV L+YH V*
Sbjct: 41 NGVWILPQGAGQQPRCSRGAHSFTRLSSICVCSSNRNKIFYR*SAMAHILGVVLVYHSV* 220
Query: 394 ANFCSNYRVASI 359
A C + R+AS+
Sbjct: 221 AYLCCSNRMASL 256
Score = 61.6 bits (128), Expect(2) = 1e-029
Identities = 36/77 (46%), Positives = 42/77 (54%)
Frame = -1 / +1
Query: 350 MQSCSLTAXWSCLGSMVLPMFTTIXXGQCL*TAK*XTYGIXXEMRS*VNLMMXXXLQRDI 171
M+SC A WSC SMVL MF +I GQ L*T+K* T GI + +N M L R+
Sbjct: 265 MESCFSIAGWSCPTSMVLRMFMSILCGQ*L*TSK**TSGIFPRRKIQINPTM*FQLHRNT 444
Query: 170 LNKTXXXHLXSSSASLR 120
NK HL S SLR
Sbjct: 445 *NKMDQKHLRLWSTSLR 495
Score = 30.9 bits (61), Expect(2) = 1e-038
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +1 / -1
Query: 517 PGKDIEVIGQHLEERSGPHLAPCSSCSR 600
P ++IEV QHLEE S PH + S+
Sbjct: 98 PRENIEVADQHLEEGSRPHCSGTPGVSK 15
>gb|CN818433.1|CN818433 HRO4460_F10_K20ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4460_F10_K20, mRNA sequence
Length = 787
Score = 271 bits (586), Expect = 1e-073
Identities = 109/142 (76%), Positives = 118/142 (83%)
Frame = -3 / +1
Query: 537 NFDVLAGPLISLAYPLYASVRAIETKNPVDDQQWLTYWVXXSFITLFELTFAPIIEWLPF 358
NFD+LAGPL+SLAYPLYASVRAIETK+ VDDQQWLTYWV SFITLFELTFAPI+EWLPF
Sbjct: 16 NFDILAGPLVSLAYPLYASVRAIETKSQVDDQQWLTYWVLYSFITLFELTFAPILEWLPF 195
Query: 357 WSYAKLFFNCXLVLPWFNGXAYVYDHFXRPMFVNRQIVNIWYIXRNEKLGKSDDVXXAAE 178
W Y KLFFNC LVLP FNG AYVY+HF RPMF+NRQIVNIWY+ R +K K DDV AAE
Sbjct: 196 WPYGKLFFNCWLVLPCFNGAAYVYEHFVRPMFLNRQIVNIWYVPRKDKSSKPDDVLSAAE 375
Query: 177 RYIEQNXXXAFXXLISKSTXXS 112
+YIE N AF LISKST S
Sbjct: 376 KYIELNGPEAFEKLISKSTKPS 441
Score = 208 bits (449), Expect = 9e-055
Identities = 101/140 (72%), Positives = 104/140 (74%)
Frame = +2 / -3
Query: 122 VDLLMSXSNAXXXFCSIYLSAAXXTSSDLPSFSFLXIYHMXTIWRFTNIGRXKWS*T*AX 301
VDLL+S SNA F SIY SAA TSS L SFL Y M TIWRF NIGR K S*T*A
Sbjct: 431 VDLLISFSNASGPFSSIYFSAAESTSSGLLDLSFLGTYQMFTIWRFKNIGRTKCS*T*AA 252
Query: 302 PLNQGKTXXQLKNSFAYDQNGSHSIIGAKVSSNKVINEXXTQ*VSHC*SSTGFFVSIALT 481
PL QGKT QLKN+F Q GSHS IGA VSSNKVINE TQ*VSHC*SST FVSIALT
Sbjct: 251 PLKQGKTSQQLKNNFP*GQKGSHSSIGANVSSNKVINEYNTQ*VSHC*SST*DFVSIALT 72
Query: 482 EAYRG*ASDMSGPARTSKLL 541
EAYRG*AS+ SGPAR SK L
Sbjct: 71 EAYRG*ASETSGPARMSKFL 12
Score = 163 bits (350), Expect = 4e-041
Identities = 82/138 (59%), Positives = 93/138 (67%)
Frame = +3 / -1
Query: 123 *TC**AXQMXXVXFVQYISLQLXXHHQIYLASHFXXYTICXLFGGSQTLAXXNGRKHRQX 302
*TC* A QM V VQY SLQL HHQ+YL F T C LFG S+TLA N RKH+Q
Sbjct: 430 *TC*LASQMLLVHSVQYTSLQLRVHHQVYLTYPFLGRTRCLLFGDSKTLAAQNARKHKQH 251
Query: 303 H*TKARPXSS*RTALHTTKMEATR*LEQKLAQTK**MSXTPSK*ATADHLQDFLSL*L*Q 482
H*+K + S+*RT H + EA + LEQ AQTK**MS TPSK*ATADHL + L L Q
Sbjct: 250 H*SKVKLASN*RTTFHRARKEAIQVLEQM*AQTK**MSTTPSK*ATADHLLEILFQLLSQ 71
Query: 483 KHIEDKQVI*VARQGHRS 536
+HIEDKQ VAR+G RS
Sbjct: 70 RHIEDKQAKLVARRGCRS 17
Score = 155 bits (332), Expect = 1e-038
Identities = 79/143 (55%), Positives = 88/143 (61%)
Frame = -2 / +3
Query: 550 GAGQ*LRCPCRATHITCLSSICFC*SYRDKKSCR*SAVAHLLGVXLIYHFV*ANFCSNYR 371
G Q LR P RAT CLSSIC C S +K S R*SAVAHLLGV LIYHFV*A+ CSN
Sbjct: 3 GTRQELRHPRRATSFACLSSICLCESN*NKISSR*SAVAHLLGVVLIYHFV*AHICSNT* 182
Query: 370 VASIXXXXXXXXXXXXXXXXVQWXCLCLRPFXXANVCEPPNSXHMVYXKK*EAR*I**CX 191
+AS +QW CLCLR F ANV E PNS H+V KK +* **C
Sbjct: 183 MASFLALWKVVLQLLASFTLLQWCCLCLRAFCAANVFESPNSKHLVRPKKG*VK*T**CT 362
Query: 190 XSCREIY*TKXTXXI*XAHQQVY 122
SCRE+Y*T+ T I* A+ QVY
Sbjct: 363 LSCREVY*TEWTRSI*EAN*QVY 431
Score = 154 bits (330), Expect(2) = 2e-039
Identities = 70/114 (61%), Positives = 82/114 (71%)
Frame = +1 / -2
Query: 124 RLADELXKCXXXVLFNISLCSXXNIIRFT*LLISXNIPYVXYLAVHKHWPXKMVVNIGXT 303
RLA+ L KC + FNI LCS IIRFT*L++S ++P V YLA+ KHWP KM+VNI T
Sbjct: 429 RLAN*LLKCFWSIQFNILLCS*EYIIRFT*LILSWDVPDVYYLAIQKHWPHKMLVNISST 250
Query: 304 IEPRQDQXAVKEQLCIRPKWKPLDNWSKS*LKQSDK*VXHPVSEPLLIIYRIFC 465
IE R + A++EQL I P+ KP WSK LKQSDK*V HPVSEPLLIIY FC
Sbjct: 249 IEAR*N*PAIEEQLSIGPERKPFKYWSKCELKQSDK*VQHPVSEPLLIIYLRFC 88
Score = 23.5 bits (45), Expect(2) = 2e-039
Identities = 6/9 (66%), Positives = 9/9 (100%)
Frame = +1 / -2
Query: 514 WPGKDIEVI 540
WPG+D+EV+
Sbjct: 39 WPGEDVEVL 13
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 7,359,964
Number of Sequences: 8143
Number of extensions: 101747
Number of successful extensions: 1114
Number of sequences better than 1.0e-020: 3
length of database: 1,567,487
effective HSP length: 43
effective length of database: 1,217,338
effective search space used: 281205078
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)