TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2922138.2.1
(1095 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN818485.1|CN818485 HRO4460_C01_E02ZS5 Lib AA070E1X Aven... 262 9e-071
gb|CN821538.1|CN821538 HRO4426_D11_G22ZS5 Lib AA069E1X Aven... 117 3e-065
gb|CN816389.1|CN816389 HRO4520_B08_D16ZS5 Lib AA071E1X Aven... 176 5e-045
gb|CN820431.1|CN820431 HRO4419_C08_F15ZS5 Lib AA069E1X Aven... 171 1e-043
>gb|CN818485.1|CN818485 HRO4460_C01_E02ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4460_C01_E02, mRNA sequence
Length = 674
Score = 262 bits (566), Expect = 9e-071
Identities = 104/163 (63%), Positives = 124/163 (76%)
Frame = +3 / +1
Query: 366 GDPYAPENVEKPFPVWIKSHTPEKGFEETKQVIGALKEKGVSAVGAAGYCWGAKVVVELA 545
GDPYAPEN ++P VWI+ H K FEE K +I +LKE+GV +GAAGYCWGAK VV+LA
Sbjct: 13 GDPYAPENADRPIQVWIQKHPQAKLFEEAKPIIASLKEQGVLTIGAAGYCWGAKGVVDLA 192
Query: 546 KAHEIQAAVLLHPSFVTVDDIKEVKCAISILGAEIDKMSPPELIKQFEQVLSLNSGVGHF 725
KA EI AAV+LHPS + VDDIKEVKC I+ILGAEID+ SPPELIK+FEQ+LS NSG+ HF
Sbjct: 193 KAREIHAAVMLHPSRIPVDDIKEVKCPIAILGAEIDQTSPPELIKEFEQILSSNSGINHF 372
Query: 726 VKIFPGVAHGWSVRYNHXXXXXXXXXXXXXXXMLDWFNKNLK* 854
VKIFPGV+HGW++RYN+ M DWF+K LK*
Sbjct: 373 VKIFPGVSHGWTMRYNNDDAAAIKSAEEALTDMTDWFDKYLK* 501
Score = 167 bits (360), Expect = 2e-042
Identities = 83/158 (52%), Positives = 92/158 (58%)
Frame = -3 / -1
Query: 859 RVYFRFLLNQSSMXXXXXXXXXXXXXXSWLYLTLHPCATPGKILTKWPTPEFKERTCSNC 680
+++FR+L NQS M S LYL +HP TPGKILTK PE +ER CSN
Sbjct: 506 QIHFRYLSNQSVMSVRASSALLIAAASSLLYLIVHP*ETPGKILTK*LIPELEERICSNS 327
Query: 679 XXXXXXXXXXXSAPRIEIAHLTSLISSTVTKEGCNSTAAWIS*AFANSTTTFAPQQYPAA 500
SAP I I H TSLISST +EGCN TAA IS AFA STT FAPQQ PAA
Sbjct: 326 LISSGGEVWSISAPSIAIGHFTSLISSTGIREGCNITAACISRAFAKSTTPFAPQQ*PAA 147
Query: 499 PTADTPFSFRAPITCFVSSNPFSGVCDFIHTGKGFSTF 386
P +TP SFR I F SSN F+ C IHT G S F
Sbjct: 146 PIVNTPCSFREAIIGFASSNNFAWGCFCIHTCIGLSAF 33
Score = 129 bits (277), Expect(2) = 2e-034
Identities = 57/115 (49%), Positives = 76/115 (66%)
Frame = -2 / -3
Query: 848 QILVEPVEHVRKGFLSTLHGSCVVVVVSHAPSMRHTREDLDEVANP*VQRKXXXXXXXXL 669
Q+LV+PV HV +G L L C++VVVSH+PSMR++REDLDEV NP*++ K
Sbjct: 495 QVLVKPVGHVCQGLLCALDSRCIIVVVSHSPSMRNSREDLDEVINP*IRGKNLLELLDQF 316
Query: 668 RWGHFVNLSSEDRNCALDLFDIVNGNKRRVQQHSSLDLMSFCQLHNNLCTPAIPC 504
G VN SS+ + LFDI++ N RR+Q +S + L SFCQ++N L TPAI C
Sbjct: 315 WRGSLVNFSSKYSDWTFHLFDIIDWNTRRMQHYSRVYLTSFCQVYNALRTPAITC 151
Score = 31.8 bits (63), Expect(2) = 2e-034
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = -2 / -3
Query: 446 FKPFFRSM*LYPHRKGLLNILRCIRIPM 363
FK F M LYPH ++I RCI IP+
Sbjct: 93 FK*FCLGMLLYPHLYWSISIFRCIWIPL 10
>gb|CN821538.1|CN821538 HRO4426_D11_G22ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4426_D11_G22, mRNA
sequence
Length = 681
Score = 117 bits (251), Expect(4) = 3e-065
Identities = 47/68 (69%), Positives = 55/68 (80%)
Frame = +3 / +3
Query: 387 NVEKPFPVWIKSHTPEKGFEETKQVIGALKEKGVSAVGAAGYCWGAKVVVELAKAHEIQA 566
N KPF WIK H PEKGFE+ K VI ALKE+GVS+VGAA YCWGAKV+ +LAK++EIQA
Sbjct: 78 NTGKPFAEWIKEHAPEKGFEKAKPVISALKEQGVSSVGAACYCWGAKVIADLAKSNEIQA 257
Query: 567 AVLLHPSF 590
AV+LH F
Sbjct: 258 AVMLHHFF 281
Score = 97.7 bits (207), Expect(4) = 1e-049
Identities = 42/64 (65%), Positives = 44/64 (68%)
Frame = -3 / -3
Query: 589 KEGCNSTAAWIS*AFANSTTTFAPQQYPAAPTADTPFSFRAPITCFVSSNPFSGVCDFIH 410
K+ CN TAAWIS FA S T APQQ AAPT DTP SFRA IT F SNPFSG C FIH
Sbjct: 280 KKWCNITAAWISLLFAKSAITLAPQQ*HAAPTLDTPCSFRAEITGFAFSNPFSGACSFIH 101
Query: 409 TGKG 398
+ G
Sbjct: 100 SANG 89
Score = 69.8 bits (146), Expect(4) = 3e-065
Identities = 30/39 (76%), Positives = 33/39 (84%)
Frame = +3 / +1
Query: 585 SFVTVDDIKEVKCAISILGAEIDKMSPPELIKQFEQVLS 701
SFVTVDDIKEVK I+ILGAE D MSPPEL+KQ + VLS
Sbjct: 277 SFVTVDDIKEVKSPIAILGAETDVMSPPELVKQIQHVLS 393
Score = 65.2 bits (136), Expect(4) = 1e-049
Identities = 27/49 (55%), Positives = 38/49 (77%)
Frame = -2 / -1
Query: 848 QILVEPVEHVRKGFLSTLHGSCVVVVVSHAPSMRHTREDLDEVANP*VQ 702
++LV+P HV +GF+S LH S V+ VVSH+PSM + RE+LD+V NP V+
Sbjct: 543 EVLVKPAIHVCQGFMSALHSS*VLAVVSHSPSMGNPRENLDKVNNPCVR 397
Score = 61.6 bits (128), Expect(4) = 3e-065
Identities = 25/53 (47%), Positives = 32/53 (60%)
Frame = +3 / +1
Query: 699 SLNSGVGHFVKIFPGVAHGWSVRYNHXXXXXXXXXXXXXXXMLDWFNKNLK*T 857
S ++ + HFVKIFPGV+HGW+VRY+ M WF+KNLK*T
Sbjct: 394 SSDTRIVHFVKIFPGVSHGWTVRYDS*YSAAVKCAHEALAHMNSWFDKNLK*T 552
Score = 51.9 bits (107), Expect(4) = 3e-065
Identities = 19/22 (86%), Positives = 22/22 (100%)
Frame = +3 / +2
Query: 318 KVASSGYFVVVPDFFHGDPYAP 383
+VASSGYFVVVPDFF+GDP+AP
Sbjct: 8 RVASSGYFVVVPDFFNGDPHAP 73
Score = 47.3 bits (97), Expect(4) = 1e-049
Identities = 18/27 (66%), Positives = 22/27 (81%)
Frame = -2 / -3
Query: 401 GLLNILRCIRIPMKEIWYHNKVP*RSN 321
GL + RC+ IP+KEIW+HNKVP RSN
Sbjct: 91 GLPVLPRCVWIPIKEIWHHNKVPRRSN 11
Score = 43.2 bits (88), Expect(4) = 2e-022
Identities = 25/68 (36%), Positives = 31/68 (45%)
Frame = -2 / -2
Query: 590 KRRVQQHSSLDLMSFCQLHNNLCTPAIPCXXXXXXXXXXXXXXXXXXLFKPFFRSM*LYP 411
K VQ +S LDL++F + N L TPAI C LFK FR M LYP
Sbjct: 281 KEVVQHNSCLDLITFR*VCNYLGTPAIACSANTRHSLLLQSRNNRFCLFKSLFRGMLLYP 102
Query: 410 HRKGLLNI 387
K ++
Sbjct: 101 FCKWFASV 78
Score = 39.6 bits (80), Expect(4) = 2e-022
Identities = 20/49 (40%), Positives = 30/49 (61%)
Frame = -1 / -3
Query: 843 SC*TSRACPQRLPQHSSRQLRRRGCISRSIHAPHQGRS*RSGQPLSSKK 697
SC TS +C L + +S+QL IS+SIH QG+S*+S Q + ++
Sbjct: 538 SCQTSYSCVPGLHERTSQQLSISCRISQSIHGKPQGKS*QSEQSVCQRR 392
Score = 39.1 bits (79), Expect(4) = 2e-022
Identities = 16/22 (72%), Positives = 17/22 (77%)
Frame = -1 / -2
Query: 387 SQVHKDPHERNLVPQQSTLTKQ 322
+QV DPH RNL PQQST TKQ
Sbjct: 77 AQVRVDPH*RNLAPQQSTQTKQ 12
Score = 38.2 bits (77), Expect(4) = 1e-049
Identities = 18/40 (45%), Positives = 22/40 (55%)
Frame = -3 / -2
Query: 703 KERTCSNCXXXXXXXXXXXSAPRIEIAHLTSLISSTVTKE 584
++RTC C SAP I + TSL+SSTVTKE
Sbjct: 395 EDRTCWICLTNSGGDITSVSAPSIAMGDFTSLMSSTVTKE 276
Score = 33.6 bits (67), Expect(4) = 2e-022
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -2 / -1
Query: 665 WGHFVNLSSEDRNCALDLFDIVNGNKR 585
W H V+ SS+ + LFD++N NKR
Sbjct: 357 WRHHVSFSSKYSDGGFHLFDVINSNKR 277
>gb|CN816389.1|CN816389 HRO4520_B08_D16ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4520_B08_D16, mRNA sequence
Length = 551
Score = 176 bits (379), Expect = 5e-045
Identities = 72/107 (67%), Positives = 84/107 (78%)
Frame = +3 / +2
Query: 537 ELAKAHEIQAAVLLHPSFVTVDDIKEVKCAISILGAEIDKMSPPELIKQFEQVLSLNSGV 716
ELAKA+EIQAAV+ HPSFVTVDDIKEVKC I++LGAE D+ SPPEL+KQFEQVLS NS +
Sbjct: 14 ELAKANEIQAAVMSHPSFVTVDDIKEVKCPIAVLGAETDRTSPPELVKQFEQVLSSNSEI 193
Query: 717 GHFVKIFPGVAHGWSVRYNHXXXXXXXXXXXXXXXMLDWFNKNLK*T 857
G+FVKIFPGV+HGW+VRY+ M +WFNKNLK*T
Sbjct: 194 GYFVKIFPGVSHGWTVRYDSDDAAAVKSAEEALTDMTNWFNKNLK*T 334
Score = 124 bits (266), Expect = 2e-029
Identities = 58/107 (54%), Positives = 64/107 (59%)
Frame = -3 / -3
Query: 856 VYFRFLLNQSSMXXXXXXXXXXXXXXSWLYLTLHPCATPGKILTKWPTPEFKERTCSNCX 677
V+ RFLLNQ M S YLT+HP TPGKILTK P E ++RTCSNC
Sbjct: 333 VHLRFLLNQLVMSVKASSALFTAAASSLSYLTVHP*DTPGKILTK*PISELEDRTCSNCL 154
Query: 676 XXXXXXXXXXSAPRIEIAHLTSLISSTVTKEGCNSTAAWIS*AFANS 536
SAP + HLTSLISSTVT EGC+ TAAWIS AFANS
Sbjct: 153 TNSGGDVLSVSAPSTAMGHLTSLISSTVTNEGCDITAAWISLAFANS 13
Score = 113 bits (241), Expect = 6e-026
Identities = 59/97 (60%), Positives = 67/97 (69%)
Frame = -1 / -1
Query: 843 SC*TSRACPQRLPQHSSRQLRRRGCISRSIHAPHQGRS*RSGQPLSSKKGPARTA*LAPV 664
SC*TS +CP RLPQ SS+Q RRR I +SIH QG+S*RS Q LS + P RTA*
Sbjct: 320 SC*TS*SCPSRLPQRSSQQPRRRCRI*QSIHETPQGKS*RSNQSLS*RIEPVRTA*PILA 141
Query: 663 GTFCQSQLRG*KLRT*PL*YRQR*QKKGATAQQPGSH 553
GTFCQ QL+ + *PL*Y Q+*Q KGAT Q PGSH
Sbjct: 140 GTFCQFQLQVQRWDI*PL*YHQQ*QMKGAT*QLPGSH 30
Score = 77.1 bits (162), Expect(2) = 3e-022
Identities = 33/49 (67%), Positives = 40/49 (81%)
Frame = -2 / -2
Query: 848 QILVEPVEHVRKGFLSTLHGSCVVVVVSHAPSMRHTREDLDEVANP*VQ 702
++LV+PV HVR+GFLS LH S VV VVS +PSMRH RE+LDEV N *V+
Sbjct: 325 KVLVKPVSHVRQGFLSALHSSRVVAVVSDSPSMRHPRENLDEVTNL*VR 179
Score = 43.2 bits (88), Expect(2) = 3e-022
Identities = 19/39 (48%), Positives = 27/39 (69%)
Frame = -2 / -2
Query: 662 GHFVNLSSEDRNCALDLFDIVNGNKRRVQQHSSLDLMSF 546
G V+ SS+ + +LFDI+N NK RV+ +S LDL+SF
Sbjct: 139 GRSVSFSSKYSDGTFNLFDIINSNK*RVRHNSCLDLISF 23
>gb|CN820431.1|CN820431 HRO4419_C08_F15ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4419_C08_F15, mRNA
sequence
Length = 560
Score = 171 bits (369), Expect = 1e-043
Identities = 69/106 (65%), Positives = 84/106 (79%)
Frame = +3 / +1
Query: 540 LAKAHEIQAAVLLHPSFVTVDDIKEVKCAISILGAEIDKMSPPELIKQFEQVLSLNSGVG 719
++K++EIQAAV+ HPSFVTVDDIKEVKC I+ILGAE D MSPPEL+KQF+QVLS N+G+G
Sbjct: 16 VSKSNEIQAAVMSHPSFVTVDDIKEVKCPIAILGAETDVMSPPELVKQFKQVLSSNAGIG 195
Query: 720 HFVKIFPGVAHGWSVRYNHXXXXXXXXXXXXXXXMLDWFNKNLK*T 857
FVKIFPGV+HGW+VRY+ M++WFNKNLK*T
Sbjct: 196 SFVKIFPGVSHGWTVRYDSNDAAAVKNAEEALADMIEWFNKNLK*T 333
Score = 117 bits (251), Expect = 2e-027
Identities = 53/108 (49%), Positives = 60/108 (55%)
Frame = -3 / -1
Query: 877 KAGCNCRVYFRFLLNQSSMXXXXXXXXXXXXXXSWLYLTLHPCATPGKILTKWPTPEFKE 698
K G +V+ RFLLN S M YLT+ P TPGKILTK P P ++
Sbjct: 353 KGGLLLQVHLRFLLNHSIMSARASSAFFTAAASLLSYLTVQP*ETPGKILTKEPIPALED 174
Query: 697 RTCSNCXXXXXXXXXXXSAPRIEIAHLTSLISSTVTKEGCNSTAAWIS 554
RTC NC SAP I + H TSLISSTVTKEGC+ TAAWIS
Sbjct: 173 RTCLNCLTNSGGDITSVSAPSIAMGHFTSLISSTVTKEGCDITAAWIS 30
Score = 97.7 bits (207), Expect = 3e-021
Identities = 56/111 (50%), Positives = 68/111 (61%)
Frame = -1 / -2
Query: 885 IITRQDAIAEFISDSC*TSRACPQRLPQHSSRQLRRRGCISRSIHAPHQGRS*RSGQPLS 706
++ ++ FI SC*T R+CP LPQ SSRQLRR IS+S H G+S*+ Q L
Sbjct: 361 LLEKEGCYFRFI*GSC*TIRSCPPGLPQRSSRQLRRCCRISQSNHEKPPGKS*QRNQSLR 182
Query: 705 SKKGPARTA*LAPVGTFCQSQLRG*KLRT*PL*YRQR*QKKGATAQQPGSH 553
+ PA TA* V T Q QL+ *+ PL*Y Q+*QKKGAT Q PGSH
Sbjct: 181 *RIEPA*TA*PILVATSRQFQLQV*RWGISPL*YHQQ*QKKGAT*QLPGSH 29
Score = 84.0 bits (177), Expect(2) = 1e-026
Identities = 32/50 (64%), Positives = 43/50 (86%)
Frame = -2 / -3
Query: 851 FQILVEPVEHVRKGFLSTLHGSCVVVVVSHAPSMRHTREDLDEVANP*VQ 702
F++LV+P +HVR+GFLS LHGSCVV VVSH+P+MR+ RE+LD+ NP V+
Sbjct: 327 FEVLVKPFDHVRQGFLSVLHGSCVVAVVSHSPTMRNPRENLDKGTNPCVR 178
Score = 51.5 bits (106), Expect(2) = 1e-026
Identities = 21/40 (52%), Positives = 28/40 (70%)
Frame = -2 / -3
Query: 665 WGHFVNLSSEDRNCALDLFDIVNGNKRRVQQHSSLDLMSF 546
W H V+ SS+ + A LFDI+N NKRRV+ +S LDL+ F
Sbjct: 141 WRHHVSFSSKYSDGAFHLFDIINSNKRRVRHNSCLDLIRF 22
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 11,200,749
Number of Sequences: 8143
Number of extensions: 146327
Number of successful extensions: 995
Number of sequences better than 1.0e-020: 5
length of database: 1,567,487
effective HSP length: 44
effective length of database: 1,209,195
effective search space used: 386942400
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)