TBLASTX 2.2.10 [Oct-19-2004]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2907809.2.1
(614 letters)
Database: Avena_nucl_with_EST.fasta
8143 sequences; 4,702,463 total letters
Score E
Sequences producing significant alignments: (bits) Value
gb|CN815299.1|CN815299 HRO4508_B07_D14ZS5 Lib AA071E1X Aven... 163 3e-044
gb|CN816820.1|CN816820 HRO4525_B10_C19ZS5 Lib AA071E1X Aven... 137 2e-036
gb|CN814648.1|CN814648 HRO4501_A03_A05ZS5 Lib AA071E1X Aven... 143 4e-036
gb|CN820796.1|CN820796 HRO4420_A02_B04ZS5 Lib AA069E1X Aven... 132 5e-035
gb|CN815018.1|CN815018 HRO4505_A01_A01ZS5 Lib AA071E1X Aven... 131 2e-032
gb|CN821631.1|CN821631 HRO4408_E02_J04ZS5 Lib AA069E1X Aven... 107 5e-028
gb|CN820713.1|CN820713 HRO4416_G01_N02ZS5 Lib AA069E1X Aven... 102 8e-027
gb|CN819298.1|CN819298 HRO4452_D02_G04ZS5 Lib AA070E1X Aven... 87 3e-022
>gb|CN815299.1|CN815299 HRO4508_B07_D14ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4508_B07_D14, mRNA sequence
Length = 470
Score = 163 bits (351), Expect(2) = 3e-044
Identities = 66/73 (90%), Positives = 69/73 (94%)
Frame = +1 / +1
Query: 139 AQKSKMARERNAEKNKGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEARHPK 318
AQKSKMARERN EK KGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEA+HPK
Sbjct: 4 AQKSKMARERNLEKLKGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEAKHPK 183
Query: 319 NDLYQCFPHLKN* 357
++L QCFPHLK *
Sbjct: 184 SELVQCFPHLKQ* 222
Score = 87.2 bits (184), Expect(3) = 4e-022
Identities = 32/40 (80%), Positives = 32/40 (80%)
Frame = -1 / -1
Query: 344 WGKHW*RSFLGCLASACSLHLASEVVQMKVCMQILHWMFM 225
WGKHW S GC ASACSLH ASEVVQM VCM ILHWMFM
Sbjct: 209 WGKHWTSSLFGCFASACSLHFASEVVQMNVCMHILHWMFM 90
Score = 30.9 bits (61), Expect(2) = 3e-044
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +3 / +1
Query: 492 MYCNPWLSACCAKNHWFCVD 551
+ CN LSACCA FCVD
Sbjct: 367 LLCNRSLSACCATIS*FCVD 426
Score = 26.3 bits (51), Expect(3) = 4e-022
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -2 / -2
Query: 226 WPSCWPPA 203
WPSCWP A
Sbjct: 91 WPSCWPRA 68
Score = 24.0 bits (46), Expect(3) = 4e-022
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = -2 / -1
Query: 556 HESTQNQWFLAQQADSQGL 500
+ STQN +AQQADS+ L
Sbjct: 431 NRSTQN*DIVAQQADSERL 375
>gb|CN816820.1|CN816820 HRO4525_B10_C19ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4525_B10_C19, mRNA sequence
Length = 416
Score = 137 bits (294), Expect(2) = 2e-036
Identities = 54/60 (90%), Positives = 57/60 (95%)
Frame = +1 / +2
Query: 178 KNKGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEARHPKNDLYQCFPHLKN* 357
K KGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEA+HPK++L QCFPHLK *
Sbjct: 11 KLKGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEAKHPKSELVQCFPHLKQ* 190
Score = 87.2 bits (184), Expect(3) = 5e-022
Identities = 32/40 (80%), Positives = 32/40 (80%)
Frame = -1 / -3
Query: 344 WGKHW*RSFLGCLASACSLHLASEVVQMKVCMQILHWMFM 225
WGKHW S GC ASACSLH ASEVVQM VCM ILHWMFM
Sbjct: 177 WGKHWTSSLFGCFASACSLHFASEVVQMNVCMHILHWMFM 58
Score = 30.9 bits (61), Expect(2) = 2e-036
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +3 / +2
Query: 492 MYCNPWLSACCAKNHWFCVD 551
+ CN LSACCA FCVD
Sbjct: 335 LLCNRSLSACCATIS*FCVD 394
Score = 26.3 bits (51), Expect(3) = 5e-022
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -2 / -1
Query: 226 WPSCWPPA 203
WPSCWP A
Sbjct: 59 WPSCWPRA 36
Score = 23.5 bits (45), Expect(3) = 5e-022
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = -2 / -3
Query: 550 STQNQWFLAQQADSQGL 500
STQN +AQQADS+ L
Sbjct: 393 STQN*DIVAQQADSERL 343
>gb|CN814648.1|CN814648 HRO4501_A03_A05ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4501_A03_A05, mRNA sequence
Length = 391
Score = 143 bits (307), Expect(2) = 4e-036
Identities = 57/65 (87%), Positives = 60/65 (92%)
Frame = +1 / +1
Query: 163 ERNAEKNKGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEARHPKNDLYQCFP 342
E N EK KGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEA+HPK++L QCFP
Sbjct: 7 EGNLEKLKGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEAKHPKSELVQCFP 186
Query: 343 HLKN* 357
HLK *
Sbjct: 187 HLKQ* 201
Score = 23.5 bits (45), Expect(2) = 4e-036
Identities = 8/12 (66%), Positives = 9/12 (75%)
Frame = +3 / +1
Query: 492 MYCNPWLSACCA 527
+ CN LSACCA
Sbjct: 346 LLCNRSLSACCA 381
>gb|CN820796.1|CN820796 HRO4420_A02_B04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4420_A02_B04, mRNA
sequence
Length = 417
Score = 132 bits (283), Expect(2) = 5e-035
Identities = 52/58 (89%), Positives = 55/58 (94%)
Frame = +1 / +3
Query: 184 KGGKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEARHPKNDLYQCFPHLKN* 357
KGGKGSQL ANKKAMNIQCKICMQTFICTTSEAKCKEHAEA+HPK++L QCFPHLK *
Sbjct: 3 KGGKGSQL*ANKKAMNIQCKICMQTFICTTSEAKCKEHAEAKHPKSELVQCFPHLKQ* 176
Score = 87.2 bits (184), Expect(3) = 5e-022
Identities = 32/40 (80%), Positives = 32/40 (80%)
Frame = -1 / -3
Query: 344 WGKHW*RSFLGCLASACSLHLASEVVQMKVCMQILHWMFM 225
WGKHW S GC ASACSLH ASEVVQM VCM ILHWMFM
Sbjct: 163 WGKHWTSSLFGCFASACSLHFASEVVQMNVCMHILHWMFM 44
Score = 30.9 bits (61), Expect(2) = 5e-035
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +3 / +3
Query: 492 MYCNPWLSACCAKNHWFCVD 551
+ CN LSACCA FCVD
Sbjct: 321 LLCNRSLSACCATIS*FCVD 380
Score = 26.3 bits (51), Expect(3) = 5e-022
Identities = 7/8 (87%), Positives = 7/8 (87%)
Frame = -2 / -1
Query: 226 WPSCWPPA 203
WPSCWP A
Sbjct: 45 WPSCWPRA 22
Score = 23.5 bits (45), Expect(3) = 5e-022
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = -2 / -3
Query: 550 STQNQWFLAQQADSQGL 500
STQN +AQQADS+ L
Sbjct: 379 STQN*DIVAQQADSERL 329
>gb|CN815018.1|CN815018 HRO4505_A01_A01ZS5 Lib AA071E1X Avena sativa cv. Ogle-C root Avena
sativa cDNA clone HRO4505_A01_A01, mRNA sequence
Length = 338
Score = 131 bits (281), Expect(2) = 2e-032
Identities = 51/56 (91%), Positives = 54/56 (96%)
Frame = +1 / +1
Query: 190 GKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEARHPKNDLYQCFPHLKN* 357
GKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEA+HPK++L QCFPHLK *
Sbjct: 1 GKGSQLEANKKAMNIQCKICMQTFICTTSEAKCKEHAEAKHPKSELVQCFPHLKQ* 168
Score = 21.7 bits (41), Expect(2) = 2e-032
Identities = 6/14 (42%), Positives = 9/14 (64%)
Frame = +3 / +2
Query: 507 WLSACCAKNHWFCV 548
W S CC+ + + CV
Sbjct: 266 WTSVCCSPSCFGCV 307
>gb|CN821631.1|CN821631 HRO4408_E02_J04ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4408_E02_J04, mRNA
sequence
Length = 357
Score = 107 bits (228), Expect(3) = 5e-028
Identities = 41/47 (87%), Positives = 44/47 (93%)
Frame = +1 / +3
Query: 217 KKAMNIQCKICMQTFICTTSEAKCKEHAEARHPKNDLYQCFPHLKN* 357
KKAM IQCKICMQTFICTTSEAKCKEHAEA+HPK++L QCFPHLK *
Sbjct: 12 KKAMYIQCKICMQTFICTTSEAKCKEHAEAKHPKSELVQCFPHLKQ* 152
Score = 30.9 bits (61), Expect(3) = 5e-028
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = +3 / +3
Query: 492 MYCNPWLSACCAKNHWFCVD 551
+ CN LSACCA FCVD
Sbjct: 297 LLCNRSLSACCATIS*FCVD 356
Score = 17.6 bits (32), Expect(3) = 5e-028
Identities = 5/11 (45%), Positives = 5/11 (45%)
Frame = +2 / +1
Query: 437 WVLGCC*CGCF 469
W CC CF
Sbjct: 250 WTSVCCSPSCF 282
>gb|CN820713.1|CN820713 HRO4416_G01_N02ZS5 Lib AA069E1X Avena sativa cv. Ogle-C etiolated
leaf Avena sativa cDNA clone HRO4416_G01_N02, mRNA
sequence
Length = 454
Score = 102 bits (218), Expect(2) = 8e-027
Identities = 38/44 (86%), Positives = 42/44 (95%)
Frame = +1 / +1
Query: 226 MNIQCKICMQTFICTTSEAKCKEHAEARHPKNDLYQCFPHLKN* 357
+NIQCKICMQTFICTTSEAKCKEHAEA+HPK++L QCFPHLK *
Sbjct: 10 VNIQCKICMQTFICTTSEAKCKEHAEAKHPKSELVQCFPHLKQ* 141
Score = 33.1 bits (66), Expect(2) = 8e-027
Identities = 15/33 (45%), Positives = 18/33 (54%)
Frame = +3 / +1
Query: 492 MYCNPWLSACCAKNHWFCVDSCCLPVLCLLNIF 590
+ CN LSACCA FCVD + C+L F
Sbjct: 286 LLCNRSLSACCATIS*FCVDLLLVLGECVLTKF 384
>gb|CN819298.1|CN819298 HRO4452_D02_G04ZS5 Lib AA070E1X Avena sativa cv. Ogle-C green leaf
Avena sativa cDNA clone HRO4452_D02_G04, mRNA sequence
Length = 336
Score = 87.2 bits (184), Expect(3) = 3e-022
Identities = 32/37 (86%), Positives = 35/37 (94%)
Frame = +1 / +1
Query: 247 CMQTFICTTSEAKCKEHAEARHPKNDLYQCFPHLKN* 357
CMQTFICTTSEAKCKEHAEA+HPK++L QCFPHLK *
Sbjct: 13 CMQTFICTTSEAKCKEHAEAKHPKSELVQCFPHLKQ* 123
Score = 25.4 bits (49), Expect(3) = 3e-022
Identities = 7/11 (63%), Positives = 9/11 (81%)
Frame = +3 / +3
Query: 480 PRLIMYCNPWL 512
P +IM+CN WL
Sbjct: 171 PSIIMFCNDWL 203
Score = 23.5 bits (45), Expect(3) = 3e-022
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +2 / +1
Query: 497 L*PLAVSLLC*KPLVLCG 550
+* +SLLC L+LCG
Sbjct: 274 M*SFTISLLCYNILILCG 327
Database: Avena_nucl_with_EST.fasta
Posted date: Feb 7, 2006 2:15 PM
Number of letters in database: 4,702,463
Number of sequences in database: 8143
Lambda K H
0.318 0.134 0.401
Matrix: BLOSUM62
Number of Hits to DB: 5,942,222
Number of Sequences: 8143
Number of extensions: 76859
Number of successful extensions: 1641
Number of sequences better than 1.0e-020: 8
length of database: 1,567,487
effective HSP length: 42
effective length of database: 1,225,481
effective search space used: 198527922
frameshift window, decay const: 40, 0.5
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 0 ( 0.0 bits)
S1: 41 (21.7 bits)